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The map label for this gene is ybjS [C]

Identifier: 146341719

GI number: 146341719

Start: 5027483

End: 5028511

Strand: Reverse

Name: ybjS [C]

Synonym: BRADO4824

Alternate gene names: 146341719

Gene position: 5028511-5027483 (Counterclockwise)

Preceding gene: 146341720

Following gene: 146341718

Centisome position: 67.44

GC content: 66.76

Gene sequence:

>1029_bases
ATGAAGGCTCTCGTCACTGGCGCCAATGGCTTTCTCGGCCGGCACGTGGTGAATGCGCTGCTGGCGCGCGGCATCGAGGT
TCGCGCGATGGTGCGTCCGGCCACGCGGGTCGAGCCATTGGGCTGGCCTGCATCCGTGGATGTGGTCAGGGCCGATCTGC
GGACCTCGCAAGATCTCGCAGGCGCCTTCGCCGATGTCGACGTGCTGATCCATCTGGCCGCCGTCGTTGCCGGCGGCGAG
GACGCGCAATTCGCCGGCACCGTCGGTGGCACCGAGCGGCTGCTCGAGGCGATGACGGCGAGCGCCTGCCGCCGCCTGGT
GCTGTGCAGCAGCTTCTCGGTCTACGACTATACGGCGACATCGGAAGTTCTCGATGAGACATCGCCGCTGCAGCAGACGC
CCGATGTCTACACCCGCGGCGGCTACACCGTCGCCAAATGGTGGCAGGAGCGGGTGACGCGACGCTATGTCGAACAGCAC
GGCTGGGATCTCACCGTCCTTCGCCCGGGCTTCATCTGGGGGCGGGGACATGGCTATCTCGCCGCGCTCGGTCAGCAGAT
CGGGCGGCATCATGTGGTGATCGGGCCGTTGACGCGGATTCCGATGACCCATGTCGAGAACTGTGCCGATGTCTTTGCGC
TCGCGGCGGCCGATCCACGGGCGCGAGGCCAGACGTTGAACATCGTCGATGGTCCTGGCGAACGGGTCTGGACCTATTTG
TCCGACTACATGCGCGGCACCGGTGAGCGCGGCTTGCGGCTGCCCGTTCCACATTGGCTGGCCAGCGCCGCGGTGCGGCT
GGCCTTTGTCACGGTCTTCCGGCGAGCGACCAAGGTGCCGGCAATCCTGACGCCGAAGAAGTTCGATGCGATGCTCAAGC
CGCTGCACTTCGACAATCGCAGGCTCAGGGAGACGCTCGGCTGGACGCCGCCGCTCGACTACAAGCAGTGCCTAGCGCGC
ACCTACGGCCCGGAGCCGGCTGCGATCGAGGCGGCTGCCTGGCCGTCGGGAAAGGCGGCGTCGATTTGA

Upstream 100 bases:

>100_bases
ATCAGGCGCTGGCGGCGAGGGCGACCTTGCCGATCACGGTCGAGGACGTGCTGTCGGTCAACCGGATGGTCGAAGCGATG
AAGCCGAGGGAACAGGTCGC

Downstream 100 bases:

>100_bases
GGTCGGCGATGACCCTGCGGCGGCCACGACGATGGAGCGCGGCGGTGGTCGCGATGATCCTGGGAGCCGTGCTCGTCGCC
GTGGTGCCCGCGACGGCGAA

Product: putative UDP-glucose 4-epimerase

Products: NA

Alternate protein names: Cholesterol dehydrogenase; 3-beta-hydroxy-Delta(5)-steroid dehydrogenase; 3-beta-HSD; 3BHSD; 3-beta hydroxysterol dehydrogenase; 3-beta-hydroxy-5-ene steroid dehydrogenase; Progesterone reductase; Steroid Delta-isomerase; Delta-5-3-ketosteroid isomerase [H]

Number of amino acids: Translated: 342; Mature: 342

Protein sequence:

>342_residues
MKALVTGANGFLGRHVVNALLARGIEVRAMVRPATRVEPLGWPASVDVVRADLRTSQDLAGAFADVDVLIHLAAVVAGGE
DAQFAGTVGGTERLLEAMTASACRRLVLCSSFSVYDYTATSEVLDETSPLQQTPDVYTRGGYTVAKWWQERVTRRYVEQH
GWDLTVLRPGFIWGRGHGYLAALGQQIGRHHVVIGPLTRIPMTHVENCADVFALAAADPRARGQTLNIVDGPGERVWTYL
SDYMRGTGERGLRLPVPHWLASAAVRLAFVTVFRRATKVPAILTPKKFDAMLKPLHFDNRRLRETLGWTPPLDYKQCLAR
TYGPEPAAIEAAAWPSGKAASI

Sequences:

>Translated_342_residues
MKALVTGANGFLGRHVVNALLARGIEVRAMVRPATRVEPLGWPASVDVVRADLRTSQDLAGAFADVDVLIHLAAVVAGGE
DAQFAGTVGGTERLLEAMTASACRRLVLCSSFSVYDYTATSEVLDETSPLQQTPDVYTRGGYTVAKWWQERVTRRYVEQH
GWDLTVLRPGFIWGRGHGYLAALGQQIGRHHVVIGPLTRIPMTHVENCADVFALAAADPRARGQTLNIVDGPGERVWTYL
SDYMRGTGERGLRLPVPHWLASAAVRLAFVTVFRRATKVPAILTPKKFDAMLKPLHFDNRRLRETLGWTPPLDYKQCLAR
TYGPEPAAIEAAAWPSGKAASI
>Mature_342_residues
MKALVTGANGFLGRHVVNALLARGIEVRAMVRPATRVEPLGWPASVDVVRADLRTSQDLAGAFADVDVLIHLAAVVAGGE
DAQFAGTVGGTERLLEAMTASACRRLVLCSSFSVYDYTATSEVLDETSPLQQTPDVYTRGGYTVAKWWQERVTRRYVEQH
GWDLTVLRPGFIWGRGHGYLAALGQQIGRHHVVIGPLTRIPMTHVENCADVFALAAADPRARGQTLNIVDGPGERVWTYL
SDYMRGTGERGLRLPVPHWLASAAVRLAFVTVFRRATKVPAILTPKKFDAMLKPLHFDNRRLRETLGWTPPLDYKQCLAR
TYGPEPAAIEAAAWPSGKAASI

Specific function: 3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidation and isomerization of cholesterol, pregnenolone, and dehydroepiandrosterone (DHEA) into cholest-4-en-3-one, progesterone, and androsterone, respectively [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-beta-HSD family [H]

Homologues:

Organism=Homo sapiens, GI193211614, Length=334, Percent_Identity=25.4491017964072, Blast_Score=73, Evalue=4e-13,
Organism=Homo sapiens, GI8393516, Length=334, Percent_Identity=25.4491017964072, Blast_Score=73, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =1.1.1.145; =5.3.3.1 [H]

Molecular weight: Translated: 37448; Mature: 37448

Theoretical pI: Translated: 9.51; Mature: 9.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKALVTGANGFLGRHVVNALLARGIEVRAMVRPATRVEPLGWPASVDVVRADLRTSQDLA
CCEEEECCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHCCHHHH
GAFADVDVLIHLAAVVAGGEDAQFAGTVGGTERLLEAMTASACRRLVLCSSFSVYDYTAT
HHHHHHHHHHHHHHHHCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECHH
SEVLDETSPLQQTPDVYTRGGYTVAKWWQERVTRRYVEQHGWDLTVLRPGFIWGRGHGYL
HHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCEEEECCCHHH
AALGQQIGRHHVVIGPLTRIPMTHVENCADVFALAAADPRARGQTLNIVDGPGERVWTYL
HHHHHHHCCCEEEECCHHHCCHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHH
SDYMRGTGERGLRLPVPHWLASAAVRLAFVTVFRRATKVPAILTPKKFDAMLKPLHFDNR
HHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEECCHHHHHHHCCCCCCCH
RLRETLGWTPPLDYKQCLARTYGPEPAAIEAAAWPSGKAASI
HHHHHCCCCCCCCHHHHHHHHCCCCCCEEEECCCCCCCCCCC
>Mature Secondary Structure
MKALVTGANGFLGRHVVNALLARGIEVRAMVRPATRVEPLGWPASVDVVRADLRTSQDLA
CCEEEECCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHCCHHHH
GAFADVDVLIHLAAVVAGGEDAQFAGTVGGTERLLEAMTASACRRLVLCSSFSVYDYTAT
HHHHHHHHHHHHHHHHCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECHH
SEVLDETSPLQQTPDVYTRGGYTVAKWWQERVTRRYVEQHGWDLTVLRPGFIWGRGHGYL
HHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCEEEECCCHHH
AALGQQIGRHHVVIGPLTRIPMTHVENCADVFALAAADPRARGQTLNIVDGPGERVWTYL
HHHHHHHCCCEEEECCHHHCCHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHH
SDYMRGTGERGLRLPVPHWLASAAVRLAFVTVFRRATKVPAILTPKKFDAMLKPLHFDNR
HHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEECCHHHHHHHCCCCCCCH
RLRETLGWTPPLDYKQCLARTYGPEPAAIEAAAWPSGKAASI
HHHHHCCCCCCCCHHHHHHHHCCCCCCEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]