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The map label for this gene is rutB [H]

Identifier: 146341174

GI number: 146341174

Start: 4449754

End: 4450437

Strand: Direct

Name: rutB [H]

Synonym: BRADO4247

Alternate gene names: 146341174

Gene position: 4449754-4450437 (Clockwise)

Preceding gene: 146341173

Following gene: 146341175

Centisome position: 59.68

GC content: 65.35

Gene sequence:

>684_bases
ATGACCCAGCGCACAATCCGCTCCGAACCCTATGCCTGGCCCTACAACGGCGATCTGCGCCCCGAGAACACCGCGCTGAT
CGTGATCGACATGCAGACCGATTTCTGCGGTGTCGGCGGCTATGTCGACAAAATGGGCTACGACCTCTCGCTGACCCGCG
CCCCGATCGAGCCGATCAAGAAGCTGCTCAGCGTAATGCGTGCGCAAGACTTCCTCATCATCCACACCCGCGAGGGCCAT
CGCACCGATCTGTCCGACCTGCCCGCCAACAAGCGCTGGCGCTCGCAGCAGATCGGCGCCGGCATTGGTGATCCCGGGCC
GTGTGGCCGCATCCTGGTGCGCGGCGAGTCGGGCTGGGACATCATCCCGGATCTGGCGCCGCTGCCCGGCGAGACCGTCA
TCGACAAGCCCGGCAAGGGCTCGTTCTGCGCCACCGATCTCGAGCTGATCCTGCGCCTGAAAGGCATCCAGAACATCGTG
CTCACGGGCATCACCACCGACGTCTGCGTCCACACCACGATGCGCGAGGGCAACGACCGCGGCTTCGAATGCGTGCTGCT
GTCAGACTGCTGCGGCGCCACCGACAAGGGCAACCACGACCACGCGCTGAAGATGATCAAGATGCAGGGCGGCGTGTTCG
GCGCCGTCGCGACCTCCTCCGCGCTGATCGAGGCGCTGTCGTGA

Upstream 100 bases:

>100_bases
GTGAGGAGCGTCGCCCTTGCGACGCGTCTCGAACCATGAGGCCCGAGACGGCGCCGCAACGCTAAATACCGATGGCCTCG
AACTCTATTGGAGCCCACCC

Downstream 100 bases:

>100_bases
TCGGCGACGATCCGACGCCGACGGGCGCGTTCGGCGTCGAGGCCATCGCGATGACGATGCGCTTCGGCGCGTTCACCGCG
CTCGACAATGTCGAGCTGAA

Product: putative isochorismatase family protein

Products: NA

Alternate protein names: Ureidoacrylate amidohydrolase [H]

Number of amino acids: Translated: 227; Mature: 226

Protein sequence:

>227_residues
MTQRTIRSEPYAWPYNGDLRPENTALIVIDMQTDFCGVGGYVDKMGYDLSLTRAPIEPIKKLLSVMRAQDFLIIHTREGH
RTDLSDLPANKRWRSQQIGAGIGDPGPCGRILVRGESGWDIIPDLAPLPGETVIDKPGKGSFCATDLELILRLKGIQNIV
LTGITTDVCVHTTMREGNDRGFECVLLSDCCGATDKGNHDHALKMIKMQGGVFGAVATSSALIEALS

Sequences:

>Translated_227_residues
MTQRTIRSEPYAWPYNGDLRPENTALIVIDMQTDFCGVGGYVDKMGYDLSLTRAPIEPIKKLLSVMRAQDFLIIHTREGH
RTDLSDLPANKRWRSQQIGAGIGDPGPCGRILVRGESGWDIIPDLAPLPGETVIDKPGKGSFCATDLELILRLKGIQNIV
LTGITTDVCVHTTMREGNDRGFECVLLSDCCGATDKGNHDHALKMIKMQGGVFGAVATSSALIEALS
>Mature_226_residues
TQRTIRSEPYAWPYNGDLRPENTALIVIDMQTDFCGVGGYVDKMGYDLSLTRAPIEPIKKLLSVMRAQDFLIIHTREGHR
TDLSDLPANKRWRSQQIGAGIGDPGPCGRILVRGESGWDIIPDLAPLPGETVIDKPGKGSFCATDLELILRLKGIQNIVL
TGITTDVCVHTTMREGNDRGFECVLLSDCCGATDKGNHDHALKMIKMQGGVFGAVATSSALIEALS

Specific function: In vivo, quickly hydrolyzes the ureidoacrylate peracid to avoid toxicity, but can also hydrolyzes ureidoacrylate that is formed spontaneously from ureidoacrylate peracid. One of the products of hydrolysis, carbamate, hydrolyzes spontaneously, thereby rele

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismatase family. RutB subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081820, Length=231, Percent_Identity=32.9004329004329, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI87081992, Length=189, Percent_Identity=29.1005291005291, Blast_Score=68, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019916
- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: NA

Molecular weight: Translated: 24696; Mature: 24565

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQRTIRSEPYAWPYNGDLRPENTALIVIDMQTDFCGVGGYVDKMGYDLSLTRAPIEPIK
CCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHCCCCEEEECCCHHHHH
KLLSVMRAQDFLIIHTREGHRTDLSDLPANKRWRSQQIGAGIGDPGPCGRILVRGESGWD
HHHHHHCCCCEEEEEECCCCCCCHHHCCCCCCCCHHHCCCCCCCCCCCCEEEEECCCCCC
IIPDLAPLPGETVIDKPGKGSFCATDLELILRLKGIQNIVLTGITTDVCVHTTMREGNDR
CCCCCCCCCCCCEECCCCCCCEEHHHHHHHHHHHCCCEEEEECCCHHHEEEEHHHCCCCC
GFECVLLSDCCGATDKGNHDHALKMIKMQGGVFGAVATSSALIEALS
CEEEEEEECCCCCCCCCCCHHHEEEEEECCCEEEEHHHHHHHHHHCC
>Mature Secondary Structure 
TQRTIRSEPYAWPYNGDLRPENTALIVIDMQTDFCGVGGYVDKMGYDLSLTRAPIEPIK
CCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHCCCCEEEECCCHHHHH
KLLSVMRAQDFLIIHTREGHRTDLSDLPANKRWRSQQIGAGIGDPGPCGRILVRGESGWD
HHHHHHCCCCEEEEEECCCCCCCHHHCCCCCCCCHHHCCCCCCCCCCCCEEEEECCCCCC
IIPDLAPLPGETVIDKPGKGSFCATDLELILRLKGIQNIVLTGITTDVCVHTTMREGNDR
CCCCCCCCCCCCEECCCCCCCEEHHHHHHHHHHHCCCEEEEECCCHHHEEEEHHHCCCCC
GFECVLLSDCCGATDKGNHDHALKMIKMQGGVFGAVATSSALIEALS
CEEEEEEECCCCCCCCCCCHHHEEEEEECCCEEEEHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA