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The map label for this gene is proS [H]

Identifier: 146341083

GI number: 146341083

Start: 4353442

End: 4354776

Strand: Reverse

Name: proS [H]

Synonym: BRADO4154

Alternate gene names: 146341083

Gene position: 4354776-4353442 (Counterclockwise)

Preceding gene: 146341084

Following gene: 146341082

Centisome position: 58.4

GC content: 64.12

Gene sequence:

>1335_bases
ATGCGTTTGTCGCGGTTTTTCCTGCCCATTCTGAAAGAAAATCCGAAGGAAGCCGAGATCGTCTCGCACCGGTTGATGCT
GCGCGCCGGCATGCTGCGGCAGGAGGCGGCCGGCATCTACGCCTGGCTGCCCCTCGGCTTCAAGGTCCTGAAGAAGATCG
AGCAGATCGTGCGCGAGGAGCAGAACCGCGCCGGCGCGCTGGAATTGCTGATGCCGACCCTGCAACTCGCCGACCTCTGG
CGCGAGAGCGGCCGCTACGACGCCTATGGTCCTGAGATGCTGCGCATCAGCGACCGTCACAAGCGTGAGTTGCTGTACGG
GCCGACCAACGAGGAGATGATCACGGAGATCTTCCGGGCCTACGTGAAGTCTTACCGCAACCTGCCGCTCAATCTCTATC
ATATCCAATGGAAATTTCGCGACGAGCAGCGTCCGCGCTTCGGCGTGATGCGCGGCCGGGAATTCCTGATGAAGGACGCC
TATTCGTTCGATCTCGACGAGGCGGGCGCGCGGCGCGCCTACAACAAGATGTTCGTCGCTTACCTGCGCACGTTTGCGCG
GATGGGCCTGAAGGGCATCCCCATGCGTGCCGAGACCGGCCCGATCGGCGGTGATCTCAGTCATGAGTTCATCGTGCTCG
CCGAAACCGGCGAGTCCGGTGTGTATTGCGACCGTAACGTGCTCGATCTGCCGATCCCGTCGGCCTCCGTCGATTATGAC
GGCGACCTCACGCCGATCATCAAGGAATGGACCTCCCTCTACGCGGCGACCGAGGACGTGCACGACGGCGCCCGCTTCGA
GGCCGAGGTGCCGGCGGAGCGGCGCGTCCACACCCGCGGCATCGAGGTCGGGCAGATCTTCTACTTCGGCACCAAATATT
CCGAGCCCATGAAGGCGCTGGTGGCCGGTCCTGATGGAGCCGAGGTCACTATCCACGGCGGCTCCTACGGCGTGGGCGTG
TCGCGTCTTGCCGGCGCCATCATCGAGGCCTGCCATGACGATGCCGGCATCAAATGGCCCGAGGAGGTCGCGCCGTTCCG
CGCCGTGATCCTCAATTTGAAACAGGGCGGCTCGGACACCGATGCCGCCTGTGAGCAGCTCTATCGCGATCTGCTCGCCA
AGGGCGTCGACGTGCTCTACGACGACACCGAGCAGCGCGCGGGGGGCAAGTTCGCCACCGCCGATCTGATCGGCATTCCC
TGGCAAATCATGGTCGGTCCGAAGAGCCTTGCCGAAGGCAAGGTCGAGGTGAAGACCCGCAGCGACGGGGCACGCGAGAT
GATGTCGCCGGCCGACATCGTGGCGCGGCTTGGCGCTGGAACCGCGGCGAGCTGA

Upstream 100 bases:

>100_bases
TACGGTGGCTTAGCCGCTTTTTCTCAATCCCAGTCTTGTGTTTTGCCGCCTGATCCGGTTTGACTGCGCCAGCCCGAACC
GAATCTCGAAGGCCCAACCC

Downstream 100 bases:

>100_bases
GCCGTTCGTTCGATCATCGTATTATCCACCGGGGCAGCCAGCCGGCCGACGGCCAATCCGGCCACAATCGGCCCGAATCA
TGGGATGATCGCGCAATGGA

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS [H]

Number of amino acids: Translated: 444; Mature: 444

Protein sequence:

>444_residues
MRLSRFFLPILKENPKEAEIVSHRLMLRAGMLRQEAAGIYAWLPLGFKVLKKIEQIVREEQNRAGALELLMPTLQLADLW
RESGRYDAYGPEMLRISDRHKRELLYGPTNEEMITEIFRAYVKSYRNLPLNLYHIQWKFRDEQRPRFGVMRGREFLMKDA
YSFDLDEAGARRAYNKMFVAYLRTFARMGLKGIPMRAETGPIGGDLSHEFIVLAETGESGVYCDRNVLDLPIPSASVDYD
GDLTPIIKEWTSLYAATEDVHDGARFEAEVPAERRVHTRGIEVGQIFYFGTKYSEPMKALVAGPDGAEVTIHGGSYGVGV
SRLAGAIIEACHDDAGIKWPEEVAPFRAVILNLKQGGSDTDAACEQLYRDLLAKGVDVLYDDTEQRAGGKFATADLIGIP
WQIMVGPKSLAEGKVEVKTRSDGAREMMSPADIVARLGAGTAAS

Sequences:

>Translated_444_residues
MRLSRFFLPILKENPKEAEIVSHRLMLRAGMLRQEAAGIYAWLPLGFKVLKKIEQIVREEQNRAGALELLMPTLQLADLW
RESGRYDAYGPEMLRISDRHKRELLYGPTNEEMITEIFRAYVKSYRNLPLNLYHIQWKFRDEQRPRFGVMRGREFLMKDA
YSFDLDEAGARRAYNKMFVAYLRTFARMGLKGIPMRAETGPIGGDLSHEFIVLAETGESGVYCDRNVLDLPIPSASVDYD
GDLTPIIKEWTSLYAATEDVHDGARFEAEVPAERRVHTRGIEVGQIFYFGTKYSEPMKALVAGPDGAEVTIHGGSYGVGV
SRLAGAIIEACHDDAGIKWPEEVAPFRAVILNLKQGGSDTDAACEQLYRDLLAKGVDVLYDDTEQRAGGKFATADLIGIP
WQIMVGPKSLAEGKVEVKTRSDGAREMMSPADIVARLGAGTAAS
>Mature_444_residues
MRLSRFFLPILKENPKEAEIVSHRLMLRAGMLRQEAAGIYAWLPLGFKVLKKIEQIVREEQNRAGALELLMPTLQLADLW
RESGRYDAYGPEMLRISDRHKRELLYGPTNEEMITEIFRAYVKSYRNLPLNLYHIQWKFRDEQRPRFGVMRGREFLMKDA
YSFDLDEAGARRAYNKMFVAYLRTFARMGLKGIPMRAETGPIGGDLSHEFIVLAETGESGVYCDRNVLDLPIPSASVDYD
GDLTPIIKEWTSLYAATEDVHDGARFEAEVPAERRVHTRGIEVGQIFYFGTKYSEPMKALVAGPDGAEVTIHGGSYGVGV
SRLAGAIIEACHDDAGIKWPEEVAPFRAVILNLKQGGSDTDAACEQLYRDLLAKGVDVLYDDTEQRAGGKFATADLIGIP
WQIMVGPKSLAEGKVEVKTRSDGAREMMSPADIVARLGAGTAAS

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro) [H]

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI34303926, Length=445, Percent_Identity=33.2584269662921, Blast_Score=238, Evalue=8e-63,
Organism=Escherichia coli, GI1786392, Length=221, Percent_Identity=57.9185520361991, Blast_Score=277, Evalue=8e-76,
Organism=Caenorhabditis elegans, GI115532348, Length=429, Percent_Identity=30.3030303030303, Blast_Score=189, Evalue=3e-48,
Organism=Caenorhabditis elegans, GI193203271, Length=89, Percent_Identity=33.7078651685393, Blast_Score=72, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6320931, Length=266, Percent_Identity=31.5789473684211, Blast_Score=147, Evalue=2e-36,
Organism=Drosophila melanogaster, GI24656200, Length=435, Percent_Identity=30.1149425287356, Blast_Score=204, Evalue=1e-52,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b [H]

EC number: =6.1.1.15 [H]

Molecular weight: Translated: 49617; Mature: 49617

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLSRFFLPILKENPKEAEIVSHRLMLRAGMLRQEAAGIYAWLPLGFKVLKKIEQIVREE
CCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHHHHH
QNRAGALELLMPTLQLADLWRESGRYDAYGPEMLRISDRHKRELLYGPTNEEMITEIFRA
HCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCHHEEECCCCHHHHHHHHHH
YVKSYRNLPLNLYHIQWKFRDEQRPRFGVMRGREFLMKDAYSFDLDEAGARRAYNKMFVA
HHHHHCCCCEEEEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
YLRTFARMGLKGIPMRAETGPIGGDLSHEFIVLAETGESGVYCDRNVLDLPIPSASVDYD
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCEECCCEEECCCCCCCCCCC
GDLTPIIKEWTSLYAATEDVHDGARFEAEVPAERRVHTRGIEVGQIFYFGTKYSEPMKAL
CCCCHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHCCCEEEEEEEECCCCCCHHHHH
VAGPDGAEVTIHGGSYGVGVSRLAGAIIEACHDDAGIKWPEEVAPFRAVILNLKQGGSDT
EECCCCCEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCH
DAACEQLYRDLLAKGVDVLYDDTEQRAGGKFATADLIGIPWQIMVGPKSLAEGKVEVKTR
HHHHHHHHHHHHHCCCCEEECCCHHHCCCCEEEEHEECCCEEEEECCHHHCCCEEEEEEC
SDGAREMMSPADIVARLGAGTAAS
CCCHHHHCCHHHHHHHHCCCCCCC
>Mature Secondary Structure
MRLSRFFLPILKENPKEAEIVSHRLMLRAGMLRQEAAGIYAWLPLGFKVLKKIEQIVREE
CCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHHHHH
QNRAGALELLMPTLQLADLWRESGRYDAYGPEMLRISDRHKRELLYGPTNEEMITEIFRA
HCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCHHEEECCCCHHHHHHHHHH
YVKSYRNLPLNLYHIQWKFRDEQRPRFGVMRGREFLMKDAYSFDLDEAGARRAYNKMFVA
HHHHHCCCCEEEEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
YLRTFARMGLKGIPMRAETGPIGGDLSHEFIVLAETGESGVYCDRNVLDLPIPSASVDYD
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCEECCCEEECCCCCCCCCCC
GDLTPIIKEWTSLYAATEDVHDGARFEAEVPAERRVHTRGIEVGQIFYFGTKYSEPMKAL
CCCCHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHCCCEEEEEEEECCCCCCHHHHH
VAGPDGAEVTIHGGSYGVGVSRLAGAIIEACHDDAGIKWPEEVAPFRAVILNLKQGGSDT
EECCCCCEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCH
DAACEQLYRDLLAKGVDVLYDDTEQRAGGKFATADLIGIPWQIMVGPKSLAEGKVEVKTR
HHHHHHHHHHHHHCCCCEEECCCHHHCCCCEEEEHEECCCEEEEECCHHHCCCEEEEEEC
SDGAREMMSPADIVARLGAGTAAS
CCCHHHHCCHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA