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The map label for this gene is tfdA [H]

Identifier: 146340959

GI number: 146340959

Start: 4220014

End: 4220940

Strand: Direct

Name: tfdA [H]

Synonym: BRADO4030

Alternate gene names: 146340959

Gene position: 4220014-4220940 (Clockwise)

Preceding gene: 146340958

Following gene: 146340960

Centisome position: 56.59

GC content: 63.97

Gene sequence:

>927_bases
GTGCGCGGGCCCCTCAATCGGTGGAAGGATTTGGCCATGAACGTCGATGCCCGCGTGCAACCAGCCTCCCAGCGATCATC
GATCGAGATCGTTCCGACAGGTGCGGCGCTCGGCGCCGAGGTCCGCAACGTCGATCTCCGGCACCTCGACGATCAGGCGT
TCGCGTCCTTGCTCCAAGCTTTCCACGACCATTCCGTGCTGCTCGTTCGCGGGCAGAACCTGACCGACCAGGACCTGATC
GCCTTCAGCCGCCGCTTCGGCGATCTGGACTTTGCTCCGATCCAGGAGAATGGCCGGCGCTTCGTTGACGGCCTGCCGGA
GATCTACATCGTCTCGAACGTGAAGGTGAATGGCGAGGCCATCGGCAGTCTCGGCGCCGGCGAAGCCGTGTGGCACACGG
ACATGTCGTATCTCGACACGCCGCCGATCGCCAGCGCGCTCTATGCGCTGGAGATTCCGCCGGTGGGCGGCAATACCTCG
TTCTGCAGCATGTACGCCGTCTACGACGCGCTTCCTGCTGATCTCAAAGCCCGCATCGCCGATCTCAAGATCAAGCATGA
CGGCACCTACAACAGCGGCGGATTCGTCCGGCAGGGCGTGACGCCCACCGATGATCCGCGCACGTCGCCTGGCGCGGTCC
ACCCGCTGGTCTGCACGCATCCCGACTCTGGCCGGCAGATGCTCTACCTCGGCCGCCGTCGCAATGCGTATCTGACGGGC
CTGGAACTCGCCGAATCGGAGGCGCTGCTCGACGAAATCTGGACCCATGTCGCGCGGCCCGCATTCGCCTGGGAGCATGC
GTGGAAGGTCGGCGATCTCGTCATCTGGGACAATCGCTGCACCATGCATCGGCGCGATCCGTTCGATGATCATTCCAGGC
GAATCATGCATCGGACCCAGATCAAGGGAACACAGCGCCCGCACTGA

Upstream 100 bases:

>100_bases
CGACTCGCGCAAGAACCTGCGCGACCGCTTCAATTTCGAGGCGGACCTGAAGCTGCCGTCGGCGTGAGCGCACGAGACTT
CGAAGACCGCCCGCGCTATT

Downstream 100 bases:

>100_bases
TCGTTCGCGCGGCGGATGCGCCCGCATGAGCGGCACAGTGCCTGCGATCGAGTCGCAAGTTGCTCGATATTTCTCCGCGT
CATGCCTTGAAGAGTCCAAG

Product: TauD/TfdA family dioxygenase

Products: Aminoacetaldehyde; CO2; H+; Sulfite; Succinate [C]

Alternate protein names: 2,4-D dioxygenase [H]

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MRGPLNRWKDLAMNVDARVQPASQRSSIEIVPTGAALGAEVRNVDLRHLDDQAFASLLQAFHDHSVLLVRGQNLTDQDLI
AFSRRFGDLDFAPIQENGRRFVDGLPEIYIVSNVKVNGEAIGSLGAGEAVWHTDMSYLDTPPIASALYALEIPPVGGNTS
FCSMYAVYDALPADLKARIADLKIKHDGTYNSGGFVRQGVTPTDDPRTSPGAVHPLVCTHPDSGRQMLYLGRRRNAYLTG
LELAESEALLDEIWTHVARPAFAWEHAWKVGDLVIWDNRCTMHRRDPFDDHSRRIMHRTQIKGTQRPH

Sequences:

>Translated_308_residues
MRGPLNRWKDLAMNVDARVQPASQRSSIEIVPTGAALGAEVRNVDLRHLDDQAFASLLQAFHDHSVLLVRGQNLTDQDLI
AFSRRFGDLDFAPIQENGRRFVDGLPEIYIVSNVKVNGEAIGSLGAGEAVWHTDMSYLDTPPIASALYALEIPPVGGNTS
FCSMYAVYDALPADLKARIADLKIKHDGTYNSGGFVRQGVTPTDDPRTSPGAVHPLVCTHPDSGRQMLYLGRRRNAYLTG
LELAESEALLDEIWTHVARPAFAWEHAWKVGDLVIWDNRCTMHRRDPFDDHSRRIMHRTQIKGTQRPH
>Mature_308_residues
MRGPLNRWKDLAMNVDARVQPASQRSSIEIVPTGAALGAEVRNVDLRHLDDQAFASLLQAFHDHSVLLVRGQNLTDQDLI
AFSRRFGDLDFAPIQENGRRFVDGLPEIYIVSNVKVNGEAIGSLGAGEAVWHTDMSYLDTPPIASALYALEIPPVGGNTS
FCSMYAVYDALPADLKARIADLKIKHDGTYNSGGFVRQGVTPTDDPRTSPGAVHPLVCTHPDSGRQMLYLGRRRNAYLTG
LELAESEALLDEIWTHVARPAFAWEHAWKVGDLVIWDNRCTMHRRDPFDDHSRRIMHRTQIKGTQRPH

Specific function: Involved in degradation of the herbicide 2,4- dichlorophenoxyacetic acid (2,4-D). Is also able to degrade 2- methyl-4-chlorophenoxyacetic acid and 3-chlorobenzoic acid [H]

COG id: COG2175

COG function: function code Q; Probable taurine catabolism dioxygenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tfdA dioxygenase family [H]

Homologues:

Organism=Escherichia coli, GI1786565, Length=285, Percent_Identity=31.5789473684211, Blast_Score=144, Evalue=6e-36,
Organism=Saccharomyces cerevisiae, GI6322971, Length=310, Percent_Identity=26.1290322580645, Blast_Score=89, Evalue=6e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003819 [H]

Pfam domain/function: PF02668 TauD [H]

EC number: 1.14.11.17 [C]

Molecular weight: Translated: 34338; Mature: 34338

Theoretical pI: Translated: 6.70; Mature: 6.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRGPLNRWKDLAMNVDARVQPASQRSSIEIVPTGAALGAEVRNVDLRHLDDQAFASLLQA
CCCCHHHHHHHHCCCCCEECCCCCCCCEEEEECCHHHCCHHHCCHHHHCCHHHHHHHHHH
FHDHSVLLVRGQNLTDQDLIAFSRRFGDLDFAPIQENGRRFVDGLPEIYIVSNVKVNGEA
HCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHCCCCCEEEEEEEEECCCC
IGSLGAGEAVWHTDMSYLDTPPIASALYALEIPPVGGNTSFCSMYAVYDALPADLKARIA
CCCCCCCCEEEECCCHHCCCCCHHHEEEEEECCCCCCCCHHHHHHHHHHCCCHHHHHEEE
DLKIKHDGTYNSGGFVRQGVTPTDDPRTSPGAVHPLVCTHPDSGRQMLYLGRRRNAYLTG
EEEEECCCCCCCCCEEECCCCCCCCCCCCCCCCCCEEEECCCCCCEEEEEECCCCEEEEC
LELAESEALLDEIWTHVARPAFAWEHAWKVGDLVIWDNRCTMHRRDPFDDHSRRIMHRTQ
HHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHH
IKGTQRPH
CCCCCCCC
>Mature Secondary Structure
MRGPLNRWKDLAMNVDARVQPASQRSSIEIVPTGAALGAEVRNVDLRHLDDQAFASLLQA
CCCCHHHHHHHHCCCCCEECCCCCCCCEEEEECCHHHCCHHHCCHHHHCCHHHHHHHHHH
FHDHSVLLVRGQNLTDQDLIAFSRRFGDLDFAPIQENGRRFVDGLPEIYIVSNVKVNGEA
HCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHCCCCCEEEEEEEEECCCC
IGSLGAGEAVWHTDMSYLDTPPIASALYALEIPPVGGNTSFCSMYAVYDALPADLKARIA
CCCCCCCCEEEECCCHHCCCCCHHHEEEEEECCCCCCCCHHHHHHHHHHCCCHHHHHEEE
DLKIKHDGTYNSGGFVRQGVTPTDDPRTSPGAVHPLVCTHPDSGRQMLYLGRRRNAYLTG
EEEEECCCCCCCCCEEECCCCCCCCCCCCCCCCCCEEEECCCCCCEEEEEECCCCEEEEC
LELAESEALLDEIWTHVARPAFAWEHAWKVGDLVIWDNRCTMHRRDPFDDHSRRIMHRTQ
HHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHH
IKGTQRPH
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Ascorbate. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-Oxoglutarate; O2; Taurine [C]

Specific reaction: 2-Oxoglutarate + O2 + Taurine --> Aminoacetaldehyde + CO2 + H+ + Sulfite + Succinate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8779585 [H]