BacMap

Definition	Bradyrhizobium sp. ORS278 chromosome, complete genome.
Accession	NC_009445
Length	7,456,587

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The map label for this gene is ppiA [H]

Identifier: 146340925

GI number: 146340925

Start: 4186763

End: 4187326

Strand: Reverse

Name: ppiA [H]

Synonym: BRADO3996

Alternate gene names: 146340925

Gene position: 4187326-4186763 (Counterclockwise)

Preceding gene: 146340926

Following gene: 146340924

Centisome position: 56.16

GC content: 65.07

Gene sequence:

>564_bases
ATGATCCGCGCTCTCGCCTTCGTCTTCGCTCTCGTGTTCGCGGTCCCGGCCCTGGCGCAGTCGCTGCCCGCCGGCCTCGA
CAAGCAGAACGCGCTGGTGATCGACACCACCAAGGGCCGCATCGTCATCAAGCTGCGCACCGATCTCGCGCCCCAGCATG
CCGAGCGGCTGAAGACCTTGGCGCGCGAGGGCTTCTACAACAACGTGCCGTTCCACCGCGTCATGGACGGCTTCATGGCG
CAGACCGGCGACGGCCAGAACTTCAACGGCACCGGCGGCTCGAAATATCCGAACCTGAAGCAGGAGTTTTCGCAGACGCC
GTTTAAGCGCGGCACCGTCGGCATGGCCCGCCGCGGCGACAGCGTCGATTCCGCCAACTCGCAGTTCTTCATCTGCTTCG
GCGATGCGTCGTTCCTCAACGGTCAGTACACGGTGATCGGCGAGGTCGTGCAGGGCATGGACGTGGTCGACAAGCTGAAG
AAGGCGCCTCCGGGCTCGGCAGGCGGCGCCGTGACCGATCCCGACAAGATGGTCAAGGTCCAGGTCGCCTCGGACACCAA
GTAG

Upstream 100 bases:

>100_bases
GTCCCGCCGTCGGTGGTGGCCCCGCTGAAAGCCAAGTTCGCCTGACGGTCCGGCGTCTTTCTAGCTCATTCCTCTCACCT
CTCATCTCATCGGAGTTGCC

Downstream 100 bases:

>100_bases
CCGGAGAGAGCAGGGCATCCGCCCGGGTTCCCGCGCCTTAAGGAAACCTTTGCCACGCGCCGCCGGTTGCATCGACAGGA
CGGGCGGCTTCAGCTTTAAG

Product: peptidyl-prolyl cis-trans isomerase A

Products: NA

Alternate protein names: PPIase; Rotamase [H]

Number of amino acids: Translated: 187; Mature: 187

Protein sequence:

>187_residues
MIRALAFVFALVFAVPALAQSLPAGLDKQNALVIDTTKGRIVIKLRTDLAPQHAERLKTLAREGFYNNVPFHRVMDGFMA
QTGDGQNFNGTGGSKYPNLKQEFSQTPFKRGTVGMARRGDSVDSANSQFFICFGDASFLNGQYTVIGEVVQGMDVVDKLK
KAPPGSAGGAVTDPDKMVKVQVASDTK

Sequences:

>Translated_187_residues
MIRALAFVFALVFAVPALAQSLPAGLDKQNALVIDTTKGRIVIKLRTDLAPQHAERLKTLAREGFYNNVPFHRVMDGFMA
QTGDGQNFNGTGGSKYPNLKQEFSQTPFKRGTVGMARRGDSVDSANSQFFICFGDASFLNGQYTVIGEVVQGMDVVDKLK
KAPPGSAGGAVTDPDKMVKVQVASDTK
>Mature_187_residues
MIRALAFVFALVFAVPALAQSLPAGLDKQNALVIDTTKGRIVIKLRTDLAPQHAERLKTLAREGFYNNVPFHRVMDGFMA
QTGDGQNFNGTGGSKYPNLKQEFSQTPFKRGTVGMARRGDSVDSANSQFFICFGDASFLNGQYTVIGEVVQGMDVVDKLK
KAPPGSAGGAVTDPDKMVKVQVASDTK

Specific function: PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides [H]

COG id: COG0652

COG function: function code O; Peptidyl-prolyl cis-trans isomerase (rotamase) - cyclophilin family

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase cyclophilin-type domain [H]

Homologues:

Organism=Homo sapiens, GI5031987, Length=130, Percent_Identity=39.2307692307692, Blast_Score=89, Evalue=3e-18,
Organism=Homo sapiens, GI19557636, Length=137, Percent_Identity=34.3065693430657, Blast_Score=82, Evalue=2e-16,
Organism=Homo sapiens, GI303305009, Length=136, Percent_Identity=35.2941176470588, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI10863927, Length=129, Percent_Identity=35.6589147286822, Blast_Score=76, Evalue=2e-14,
Organism=Homo sapiens, GI45439316, Length=127, Percent_Identity=35.4330708661417, Blast_Score=75, Evalue=4e-14,
Organism=Homo sapiens, GI7706339, Length=131, Percent_Identity=32.0610687022901, Blast_Score=74, Evalue=6e-14,
Organism=Homo sapiens, GI5174637, Length=126, Percent_Identity=34.9206349206349, Blast_Score=73, Evalue=1e-13,
Organism=Homo sapiens, GI24308049, Length=137, Percent_Identity=34.3065693430657, Blast_Score=71, Evalue=7e-13,
Organism=Homo sapiens, GI4758950, Length=134, Percent_Identity=35.0746268656716, Blast_Score=70, Evalue=9e-13,
Organism=Homo sapiens, GI310129267, Length=130, Percent_Identity=33.8461538461538, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI310120620, Length=130, Percent_Identity=33.8461538461538, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI4505991, Length=134, Percent_Identity=35.8208955223881, Blast_Score=69, Evalue=2e-12,
Organism=Homo sapiens, GI310110104, Length=128, Percent_Identity=33.59375, Blast_Score=69, Evalue=2e-12,
Organism=Homo sapiens, GI150418007, Length=142, Percent_Identity=34.5070422535211, Blast_Score=68, Evalue=5e-12,
Organism=Homo sapiens, GI4826932, Length=131, Percent_Identity=32.824427480916, Blast_Score=68, Evalue=5e-12,
Organism=Homo sapiens, GI20911035, Length=150, Percent_Identity=32, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI64276486, Length=122, Percent_Identity=39.344262295082, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI256017253, Length=129, Percent_Identity=35.6589147286822, Blast_Score=66, Evalue=1e-11,
Organism=Homo sapiens, GI221139811, Length=129, Percent_Identity=35.6589147286822, Blast_Score=66, Evalue=1e-11,
Organism=Homo sapiens, GI256017251, Length=129, Percent_Identity=35.6589147286822, Blast_Score=66, Evalue=1e-11,
Organism=Homo sapiens, GI5454154, Length=138, Percent_Identity=32.6086956521739, Blast_Score=66, Evalue=2e-11,
Organism=Homo sapiens, GI169163387, Length=140, Percent_Identity=31.4285714285714, Blast_Score=65, Evalue=3e-11,
Organism=Homo sapiens, GI169164185, Length=140, Percent_Identity=31.4285714285714, Blast_Score=65, Evalue=3e-11,
Organism=Homo sapiens, GI169163623, Length=140, Percent_Identity=31.4285714285714, Blast_Score=65, Evalue=3e-11,
Organism=Homo sapiens, GI178057341, Length=132, Percent_Identity=34.0909090909091, Blast_Score=65, Evalue=4e-11,
Organism=Homo sapiens, GI209417936, Length=132, Percent_Identity=34.0909090909091, Blast_Score=64, Evalue=6e-11,
Organism=Homo sapiens, GI30102944, Length=132, Percent_Identity=34.0909090909091, Blast_Score=64, Evalue=6e-11,
Organism=Homo sapiens, GI219842199, Length=132, Percent_Identity=34.0909090909091, Blast_Score=64, Evalue=6e-11,
Organism=Homo sapiens, GI310127646, Length=127, Percent_Identity=32.2834645669291, Blast_Score=64, Evalue=7e-11,
Organism=Homo sapiens, GI310114371, Length=127, Percent_Identity=32.2834645669291, Blast_Score=64, Evalue=7e-11,
Organism=Homo sapiens, GI310119221, Length=127, Percent_Identity=32.2834645669291, Blast_Score=64, Evalue=7e-11,
Organism=Homo sapiens, GI22547212, Length=179, Percent_Identity=29.608938547486, Blast_Score=64, Evalue=8e-11,
Organism=Homo sapiens, GI7657473, Length=179, Percent_Identity=29.608938547486, Blast_Score=64, Evalue=8e-11,
Organism=Homo sapiens, GI22547215, Length=179, Percent_Identity=29.608938547486, Blast_Score=64, Evalue=8e-11,
Organism=Escherichia coli, GI1786736, Length=155, Percent_Identity=40.6451612903226, Blast_Score=94, Evalue=7e-21,
Organism=Escherichia coli, GI1789763, Length=188, Percent_Identity=37.7659574468085, Blast_Score=93, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17539498, Length=129, Percent_Identity=39.5348837209302, Blast_Score=84, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17506311, Length=139, Percent_Identity=38.1294964028777, Blast_Score=84, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17552780, Length=182, Percent_Identity=32.4175824175824, Blast_Score=77, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI71980594, Length=134, Percent_Identity=35.0746268656716, Blast_Score=76, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI71980590, Length=134, Percent_Identity=35.0746268656716, Blast_Score=76, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17552782, Length=149, Percent_Identity=32.8859060402685, Blast_Score=75, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17539496, Length=147, Percent_Identity=32.6530612244898, Blast_Score=74, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17566266, Length=137, Percent_Identity=32.8467153284672, Blast_Score=74, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17565860, Length=162, Percent_Identity=35.8024691358025, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17532643, Length=141, Percent_Identity=34.0425531914894, Blast_Score=70, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17559074, Length=133, Percent_Identity=33.8345864661654, Blast_Score=68, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17506307, Length=130, Percent_Identity=36.9230769230769, Blast_Score=67, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17532641, Length=141, Percent_Identity=31.9148936170213, Blast_Score=65, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6320359, Length=140, Percent_Identity=39.2857142857143, Blast_Score=80, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6323562, Length=156, Percent_Identity=33.3333333333333, Blast_Score=73, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6321848, Length=133, Percent_Identity=36.8421052631579, Blast_Score=70, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6320510, Length=140, Percent_Identity=36.4285714285714, Blast_Score=66, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6323246, Length=135, Percent_Identity=35.5555555555556, Blast_Score=64, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24664125, Length=130, Percent_Identity=41.5384615384615, Blast_Score=94, Evalue=4e-20,
Organism=Drosophila melanogaster, GI21357783, Length=130, Percent_Identity=41.5384615384615, Blast_Score=94, Evalue=4e-20,
Organism=Drosophila melanogaster, GI45549139, Length=130, Percent_Identity=41.5384615384615, Blast_Score=92, Evalue=2e-19,
Organism=Drosophila melanogaster, GI17647301, Length=137, Percent_Identity=37.956204379562, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI17986117, Length=177, Percent_Identity=30.5084745762712, Blast_Score=80, Evalue=8e-16,
Organism=Drosophila melanogaster, GI24652460, Length=136, Percent_Identity=33.0882352941176, Blast_Score=73, Evalue=1e-13,
Organism=Drosophila melanogaster, GI17136360, Length=127, Percent_Identity=33.8582677165354, Blast_Score=68, Evalue=3e-12,
Organism=Drosophila melanogaster, GI19922376, Length=144, Percent_Identity=29.8611111111111, Blast_Score=67, Evalue=9e-12,
Organism=Drosophila melanogaster, GI24650807, Length=139, Percent_Identity=35.9712230215827, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI19922912, Length=156, Percent_Identity=32.6923076923077, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI20130249, Length=131, Percent_Identity=34.3511450381679, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24661775, Length=136, Percent_Identity=31.6176470588235, Blast_Score=64, Evalue=5e-11,
Organism=Drosophila melanogaster, GI21355237, Length=136, Percent_Identity=31.6176470588235, Blast_Score=64, Evalue=5e-11,
Organism=Drosophila melanogaster, GI24661771, Length=136, Percent_Identity=31.6176470588235, Blast_Score=64, Evalue=5e-11,
Organism=Drosophila melanogaster, GI21357249, Length=123, Percent_Identity=36.5853658536585, Blast_Score=64, Evalue=6e-11,

Paralogues:

None

Copy number: 780 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015891
- InterPro:   IPR020892
- InterPro:   IPR002130 [H]

Pfam domain/function: PF00160 Pro_isomerase [H]

EC number: =5.2.1.8 [H]

Molecular weight: Translated: 20131; Mature: 20131

Theoretical pI: Translated: 9.99; Mature: 9.99

Prosite motif: PS00170 CSA_PPIASE_1 ; PS50072 CSA_PPIASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRALAFVFALVFAVPALAQSLPAGLDKQNALVIDTTKGRIVIKLRTDLAPQHAERLKTL
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCEEEEEEECCCCHHHHHHHHHH
AREGFYNNVPFHRVMDGFMAQTGDGQNFNGTGGSKYPNLKQEFSQTPFKRGTVGMARRGD
HHHCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCHHCCCC
SVDSANSQFFICFGDASFLNGQYTVIGEVVQGMDVVDKLKKAPPGSAGGAVTDPDKMVKV
CCCCCCCEEEEEECCCHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEE
QVASDTK
EECCCCC
>Mature Secondary Structure
MIRALAFVFALVFAVPALAQSLPAGLDKQNALVIDTTKGRIVIKLRTDLAPQHAERLKTL
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCEEEEEEECCCCHHHHHHHHHH
AREGFYNNVPFHRVMDGFMAQTGDGQNFNGTGGSKYPNLKQEFSQTPFKRGTVGMARRGD
HHHCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCHHCCCC
SVDSANSQFFICFGDASFLNGQYTVIGEVVQGMDVVDKLKKAPPGSAGGAVTDPDKMVKV
CCCCCCCEEEEEECCCHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEE
QVASDTK
EECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA