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The map label for this gene is cysB [H]

Identifier: 146340846

GI number: 146340846

Start: 4086608

End: 4087642

Strand: Direct

Name: cysB [H]

Synonym: BRADO3909

Alternate gene names: 146340846

Gene position: 4086608-4087642 (Clockwise)

Preceding gene: 146340845

Following gene: 146340851

Centisome position: 54.81

GC content: 64.64

Gene sequence:

>1035_bases
ATGACCATTAGAAACGACGTCGTCGCGGCGATCGGCAACACGCCCCTGATCAAGCTCACGCGCGCCTCGGAGGCGACCGG
CTGCACCATCCTCGGCAAGGCCGAGTTCATGAACCCCGGCCAGTCGGTCAAGGATCGCGCCGGCAAATGGATGATCCTGG
AGGCCGAGAAGCGCGGCGATCTCAAGCCGGGCGGCCTCGTCGTGGAGTCAACGGCCGGCAACACCGGCATCGGTCTTGCC
GTGGTCGCCAATGCCCGTGGCTATCGCACGCTGATCGTGATTCCGGAGACGCAGAGCCAGGAAAAGAAGGACATGCTGCG
GATGTGCGGTGCCGATCTCGTCGAGGTCCCGGCTCTGCCGTTCAACAACCCGAACAATTACGCCCATGTCGGCCGCCGGC
TTGCCGACGAGTTGCGCAAGAGCGAGCCGAACGGCGTGCTGTTCGCCGACCAGTGGAACAACCTCGACAACGCCAAGGCG
CATTACGAGTCGACCGGTCCGGAGATCTGGGAGCAGACCGGCGGCAAGGTCGACGGCTTCGTCTGCGCGGTCGGCACCGG
CGGCACGCTGGCCGGCACCAGCCGCTATCTGAAGGAGAAAAACGCCGGCATCGTCACAGCCTGCGCCGATCCGCACGGCT
TTGCGATGTACAACTGGTTCAAGAACCGCGAGGTCAAGTCCACGCCGGGCGATTCCATCACCGAGGGCATCGGCATCGGC
CGGGTCACGCCGGTGATCGAGACCGCCAAGGTCGATACCGCGTTCCTGATCTCCGATGAGGACGCGGTCAACACCATCTA
CGATCTGCTGCAGCACGAAGGCCTGTGCCTGGGCGGCTCGAGCGGCGTCAATATCGCCGGCGCGGTGCAGCTCGCCAAGC
AGCTTGGGCCCGGCCACACCATCGTGACCATCCTGTGCGACCCGGGCAACCGCTATCAGTCGAAGCTGTACAATCCGGAG
TTCATGCGGTCGAAGAACCTGCCCGTGCCGGAATGGCTGGAGCGGCGCAGCGGCATCAAGCCGCCATTCGTGTGA

Upstream 100 bases:

>100_bases
CAAATCCGGGGCATAGTGCCGCTGGCTTACGCAGCACTGGAGACTAGGCTAGGGTCTTCCTAGATCTGCTCTTAAGGAAG
CTGCCCAAGGTATTTGCAAT

Downstream 100 bases:

>100_bases
GAGCGAATAGCGAATAGCGAGTAGCGAGTAGCGAGTAGCGAGTAGCGAGTAGCGAGTAGCGAGCAGATACTGCTCGCTGA
CGGGGCATCAACCCCTATTC

Product: cysteine synthase A

Products: NA

Alternate protein names: CSase B; O-acetylserine (thiol)-lyase B; OAS-TL B; O-acetylserine sulfhydrylase B [H]

Number of amino acids: Translated: 344; Mature: 343

Protein sequence:

>344_residues
MTIRNDVVAAIGNTPLIKLTRASEATGCTILGKAEFMNPGQSVKDRAGKWMILEAEKRGDLKPGGLVVESTAGNTGIGLA
VVANARGYRTLIVIPETQSQEKKDMLRMCGADLVEVPALPFNNPNNYAHVGRRLADELRKSEPNGVLFADQWNNLDNAKA
HYESTGPEIWEQTGGKVDGFVCAVGTGGTLAGTSRYLKEKNAGIVTACADPHGFAMYNWFKNREVKSTPGDSITEGIGIG
RVTPVIETAKVDTAFLISDEDAVNTIYDLLQHEGLCLGGSSGVNIAGAVQLAKQLGPGHTIVTILCDPGNRYQSKLYNPE
FMRSKNLPVPEWLERRSGIKPPFV

Sequences:

>Translated_344_residues
MTIRNDVVAAIGNTPLIKLTRASEATGCTILGKAEFMNPGQSVKDRAGKWMILEAEKRGDLKPGGLVVESTAGNTGIGLA
VVANARGYRTLIVIPETQSQEKKDMLRMCGADLVEVPALPFNNPNNYAHVGRRLADELRKSEPNGVLFADQWNNLDNAKA
HYESTGPEIWEQTGGKVDGFVCAVGTGGTLAGTSRYLKEKNAGIVTACADPHGFAMYNWFKNREVKSTPGDSITEGIGIG
RVTPVIETAKVDTAFLISDEDAVNTIYDLLQHEGLCLGGSSGVNIAGAVQLAKQLGPGHTIVTILCDPGNRYQSKLYNPE
FMRSKNLPVPEWLERRSGIKPPFV
>Mature_343_residues
TIRNDVVAAIGNTPLIKLTRASEATGCTILGKAEFMNPGQSVKDRAGKWMILEAEKRGDLKPGGLVVESTAGNTGIGLAV
VANARGYRTLIVIPETQSQEKKDMLRMCGADLVEVPALPFNNPNNYAHVGRRLADELRKSEPNGVLFADQWNNLDNAKAH
YESTGPEIWEQTGGKVDGFVCAVGTGGTLAGTSRYLKEKNAGIVTACADPHGFAMYNWFKNREVKSTPGDSITEGIGIGR
VTPVIETAKVDTAFLISDEDAVNTIYDLLQHEGLCLGGSSGVNIAGAVQLAKQLGPGHTIVTILCDPGNRYQSKLYNPEF
MRSKNLPVPEWLERRSGIKPPFV

Specific function: Two cysteine synthase enzymes are found. Both catalyze the same reaction. Cysteine synthase B can also use thiosulfate in place of sulfide to give cysteine thiosulfonate as a product [H]

COG id: COG0031

COG function: function code E; Cysteine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cysteine synthase/cystathionine beta- synthase family [H]

Homologues:

Organism=Homo sapiens, GI295821202, Length=328, Percent_Identity=39.6341463414634, Blast_Score=220, Evalue=1e-57,
Organism=Homo sapiens, GI295821200, Length=328, Percent_Identity=39.6341463414634, Blast_Score=220, Evalue=1e-57,
Organism=Homo sapiens, GI4557415, Length=328, Percent_Identity=39.6341463414634, Blast_Score=220, Evalue=1e-57,
Organism=Escherichia coli, GI2367138, Length=304, Percent_Identity=38.8157894736842, Blast_Score=202, Evalue=3e-53,
Organism=Escherichia coli, GI1788754, Length=329, Percent_Identity=37.9939209726444, Blast_Score=172, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI17535051, Length=313, Percent_Identity=36.4217252396166, Blast_Score=182, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI115535073, Length=319, Percent_Identity=36.9905956112853, Blast_Score=172, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI17562970, Length=316, Percent_Identity=37.0253164556962, Blast_Score=169, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI32566674, Length=309, Percent_Identity=37.2168284789644, Blast_Score=168, Evalue=4e-42,
Organism=Caenorhabditis elegans, GI17534315, Length=337, Percent_Identity=34.7181008902077, Blast_Score=158, Evalue=3e-39,
Organism=Caenorhabditis elegans, GI17561720, Length=322, Percent_Identity=34.7826086956522, Blast_Score=152, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI25147552, Length=339, Percent_Identity=33.0383480825959, Blast_Score=144, Evalue=9e-35,
Organism=Caenorhabditis elegans, GI32566672, Length=232, Percent_Identity=32.7586206896552, Blast_Score=99, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI71996324, Length=334, Percent_Identity=26.9461077844311, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17539800, Length=350, Percent_Identity=24.2857142857143, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6321449, Length=324, Percent_Identity=38.8888888888889, Blast_Score=195, Evalue=1e-50,
Organism=Saccharomyces cerevisiae, GI6321594, Length=344, Percent_Identity=34.8837209302326, Blast_Score=187, Evalue=3e-48,
Organism=Drosophila melanogaster, GI24643623, Length=332, Percent_Identity=36.4457831325301, Blast_Score=174, Evalue=6e-44,
Organism=Drosophila melanogaster, GI20129101, Length=332, Percent_Identity=36.4457831325301, Blast_Score=174, Evalue=6e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001216
- InterPro:   IPR005856
- InterPro:   IPR005858
- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =2.5.1.47 [H]

Molecular weight: Translated: 37039; Mature: 36907

Theoretical pI: Translated: 7.01; Mature: 7.01

Prosite motif: PS00901 CYS_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIRNDVVAAIGNTPLIKLTRASEATGCTILGKAEFMNPGQSVKDRAGKWMILEAEKRGD
CCCCCCCCEECCCCCEEEEEECCCCCCEEEEECHHHCCCCCHHHHHCCCEEEEEECCCCC
LKPGGLVVESTAGNTGIGLAVVANARGYRTLIVIPETQSQEKKDMLRMCGADLVEVPALP
CCCCCEEEECCCCCCCCEEEEEECCCCCEEEEEEECCCCHHHHHHHHHCCCCHHHCCCCC
FNNPNNYAHVGRRLADELRKSEPNGVLFADQWNNLDNAKAHYESTGPEIWEQTGGKVDGF
CCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCHHHCCCCCHHHHHHCCCCCCEE
VCAVGTGGTLAGTSRYLKEKNAGIVTACADPHGFAMYNWFKNREVKSTPGDSITEGIGIG
EEEECCCCCCHHHHHHHHHCCCCEEEEECCCCCCEEEHHHCCCCCCCCCCCHHHHCCCCC
RVTPVIETAKVDTAFLISDEDAVNTIYDLLQHEGLCLGGSSGVNIAGAVQLAKQLGPGHT
CCCCCEEECCCCEEEEECCCHHHHHHHHHHHHCCEEEECCCCCCHHHHHHHHHHCCCCCE
IVTILCDPGNRYQSKLYNPEFMRSKNLPVPEWLERRSGIKPPFV
EEEEEECCCCHHHHHCCCHHHHHCCCCCCHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TIRNDVVAAIGNTPLIKLTRASEATGCTILGKAEFMNPGQSVKDRAGKWMILEAEKRGD
CCCCCCCEECCCCCEEEEEECCCCCCEEEEECHHHCCCCCHHHHHCCCEEEEEECCCCC
LKPGGLVVESTAGNTGIGLAVVANARGYRTLIVIPETQSQEKKDMLRMCGADLVEVPALP
CCCCCEEEECCCCCCCCEEEEEECCCCCEEEEEEECCCCHHHHHHHHHCCCCHHHCCCCC
FNNPNNYAHVGRRLADELRKSEPNGVLFADQWNNLDNAKAHYESTGPEIWEQTGGKVDGF
CCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCHHHCCCCCHHHHHHCCCCCCEE
VCAVGTGGTLAGTSRYLKEKNAGIVTACADPHGFAMYNWFKNREVKSTPGDSITEGIGIG
EEEECCCCCCHHHHHHHHHCCCCEEEEECCCCCCEEEHHHCCCCCCCCCCCHHHHCCCCC
RVTPVIETAKVDTAFLISDEDAVNTIYDLLQHEGLCLGGSSGVNIAGAVQLAKQLGPGHT
CCCCCEEECCCCEEEEECCCHHHHHHHHHHHHCCEEEECCCCCCHHHHHHHHHHCCCCCE
IVTILCDPGNRYQSKLYNPEFMRSKNLPVPEWLERRSGIKPPFV
EEEEEECCCCHHHHHCCCHHHHHCCCCCCHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2188958; 9205837; 9278503 [H]