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The map label for this gene is pcm [H]

Identifier: 146340823

GI number: 146340823

Start: 4060130

End: 4060795

Strand: Reverse

Name: pcm [H]

Synonym: BRADO3885

Alternate gene names: 146340823

Gene position: 4060795-4060130 (Counterclockwise)

Preceding gene: 146340824

Following gene: 146340822

Centisome position: 54.46

GC content: 64.56

Gene sequence:

>666_bases
ATGTCTTCATTCGCCACGGCGCGTCAGAAAATGGTCGATGGTCAGGTTCGGACCAACGACGTCACCGACCACCGCGTGCT
GGATGCGTTGTTGACCGTGCCGCGGGAAGCCTATGTGCCCGCGGATCGGCAGGCGCTCGCCTATCTCGATATGGATCTCG
ACGTCGCCGAAGCTGGCGCTGCAAAGCGCTTTCTGATCAAGCCGATGCTGACCGGGAAGCTGTTGCAGGCGGCAGAAATC
AAGCCGACGGATGATGTGCTCGTCGTCGGCTGTGCGACCGGCTATCTCGCGGCGCTCGCCGCCAGGCTGGCCGGCAGCGT
GACTGGGACAGAAGTTGAGCCCGCTCTCGAAGGACGTGCCAAGCAATTGCTAGCCGGCGCTGCTGGCTGCCCGATCACGA
TTCGCGTCGCACCGGCCGTGCAGGGTGATCCGGCGGACGGCCGCTTCGACGTGATTCTCCTGAACGGGGCCACCGAGATC
GCCGTCGACGGGCTTTGCCACCAGCTCAAGGAGGGTGGCCGTCTGGTCGGCGTGTTTGCCAAGAACCGGCCCGCCCGTGC
CATGATCTTTACGCGATCGCATGGTGACGTCGGCAGCAGGGTCCTGTTCGATGCCGCGGCGCCGGTGGTTCCCGGCCTCG
AGCGCGTTTCCGAGTTCGTGTTCTAA

Upstream 100 bases:

>100_bases
GTGTCTCCGATAGAGCCCCGTGTTGCCTTTTCGAAAGCCTTGTACCAAAAGACTGCGCAGATCGTTCGCGGTTCCCACCG
CCTTGCTTGCAGGCCTGCTC

Downstream 100 bases:

>100_bases
GCCAAGGCTTTCCTGGGTCCCCTGCGGTTTGGGGCGCACGCGATGATCGCGTTGCGCAATCGGACGGTGCTGAGTCGTCT
TCGCGGCTTTGGGTCGTCGA

Product: putative protein-L-isoaspartate O-methyltransferase

Products: NA

Alternate protein names: L-isoaspartyl protein carboxyl methyltransferase; Protein L-isoaspartyl methyltransferase; Protein-beta-aspartate methyltransferase; PIMT [H]

Number of amino acids: Translated: 221; Mature: 220

Protein sequence:

>221_residues
MSSFATARQKMVDGQVRTNDVTDHRVLDALLTVPREAYVPADRQALAYLDMDLDVAEAGAAKRFLIKPMLTGKLLQAAEI
KPTDDVLVVGCATGYLAALAARLAGSVTGTEVEPALEGRAKQLLAGAAGCPITIRVAPAVQGDPADGRFDVILLNGATEI
AVDGLCHQLKEGGRLVGVFAKNRPARAMIFTRSHGDVGSRVLFDAAAPVVPGLERVSEFVF

Sequences:

>Translated_221_residues
MSSFATARQKMVDGQVRTNDVTDHRVLDALLTVPREAYVPADRQALAYLDMDLDVAEAGAAKRFLIKPMLTGKLLQAAEI
KPTDDVLVVGCATGYLAALAARLAGSVTGTEVEPALEGRAKQLLAGAAGCPITIRVAPAVQGDPADGRFDVILLNGATEI
AVDGLCHQLKEGGRLVGVFAKNRPARAMIFTRSHGDVGSRVLFDAAAPVVPGLERVSEFVF
>Mature_220_residues
SSFATARQKMVDGQVRTNDVTDHRVLDALLTVPREAYVPADRQALAYLDMDLDVAEAGAAKRFLIKPMLTGKLLQAAEIK
PTDDVLVVGCATGYLAALAARLAGSVTGTEVEPALEGRAKQLLAGAAGCPITIRVAPAVQGDPADGRFDVILLNGATEIA
VDGLCHQLKEGGRLVGVFAKNRPARAMIFTRSHGDVGSRVLFDAAAPVVPGLERVSEFVF

Specific function: Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins [H]

COG id: COG2518

COG function: function code O; Protein-L-isoaspartate carboxylmethyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. L- isoaspartyl/D-aspartyl protein methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789100, Length=168, Percent_Identity=28.5714285714286, Blast_Score=67, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000682 [H]

Pfam domain/function: PF01135 PCMT [H]

EC number: =2.1.1.77 [H]

Molecular weight: Translated: 23278; Mature: 23147

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSFATARQKMVDGQVRTNDVTDHRVLDALLTVPREAYVPADRQALAYLDMDLDVAEAGA
CCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCHHHCCCCCCCEEEEEECCCCHHHCCC
AKRFLIKPMLTGKLLQAAEIKPTDDVLVVGCATGYLAALAARLAGSVTGTEVEPALEGRA
CHHHHHHHHHHHHHHHHCCCCCCCCEEEEECHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
KQLLAGAAGCPITIRVAPAVQGDPADGRFDVILLNGATEIAVDGLCHQLKEGGRLVGVFA
HHHHHCCCCCCEEEEEECCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHCCCEEEEEEE
KNRPARAMIFTRSHGDVGSRVLFDAAAPVVPGLERVSEFVF
CCCCCEEEEEEECCCCCCCEEHHHHCCCCCCCHHHHHHHCC
>Mature Secondary Structure 
SSFATARQKMVDGQVRTNDVTDHRVLDALLTVPREAYVPADRQALAYLDMDLDVAEAGA
CCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCHHHCCCCCCCEEEEEECCCCHHHCCC
AKRFLIKPMLTGKLLQAAEIKPTDDVLVVGCATGYLAALAARLAGSVTGTEVEPALEGRA
CHHHHHHHHHHHHHHHHCCCCCCCCEEEEECHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
KQLLAGAAGCPITIRVAPAVQGDPADGRFDVILLNGATEIAVDGLCHQLKEGGRLVGVFA
HHHHHCCCCCCEEEEEECCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHCCCEEEEEEE
KNRPARAMIFTRSHGDVGSRVLFDAAAPVVPGLERVSEFVF
CCCCCEEEEEEECCCCCCCEEHHHHCCCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA