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The map label for this gene is ispDF [H]

Identifier: 146340810

GI number: 146340810

Start: 4044983

End: 4046164

Strand: Direct

Name: ispDF [H]

Synonym: BRADO3869

Alternate gene names: 146340810

Gene position: 4044983-4046164 (Clockwise)

Preceding gene: 146340804

Following gene: 146340811

Centisome position: 54.25

GC content: 68.27

Gene sequence:

>1182_bases
ATGACGACTTCCCAGCGGACTGCAGCCATTCTCGTCGCGGCCGGACGTGGCCTGCGCGCCGGCGCCGGCGGTCCGAAGCA
ATACCGGACCATCGGCGGCCGCACCGTGATCCATCGCGCGCTCGCTGCCTTCGCGGAGCATCCCGAAGTGGCCGTGGTGC
AGCCGGTGGTGAACCCCGATGACATCGACGTCTTCAATGCCGCCGTCGCCGGCCTACGTCACGAAGCGCCCGCGCGTGGT
GGCGCGACACGCCAGGCCTCGGTGCTCGCTGGCCTTGAGGCGCTGGTGCCGCACAAGCCTGAGATCGTGCTGATCCACGA
CGCGGCCCGTCCGTTCGTGACGCCGGCCGTGATCTCGCGCGCGATCATCGCTGCGAACAAGACCGGCGCCGCGATCCCCG
TCGCGCCCGTCACCGATACGATCAAAGAGGTCGGCGCGAGCGGTGACATCACCGCGACGCCGGAACGTGCAAAACTGCGC
ATCGCGCAGACGCCGCAGACTTTCAGGTTCGACACGATCCTGGAGGCGCATCGCCGCGCGGCGCGCGAGGGCCTCACCGA
GTTCACGGATGATGCGGCGATCGCCGAATGGGCGGGATTGACCGTCGCGACCTTTGAGGGCGATGTTGCCAACATGAAGC
TCACCACACCGGAAGATTTCGTGCGCGAGGAAGCGCGGCTGGCCGCATCGCTCGGCGACATCCGAACCGGCACCGGCTAC
GACGTGCACGCCTTCGGCGAAGGCGACCATGTCTGGCTGTGCGGCCTGCGCGTACCGCATACGAAAGGCTTCCTCGCCCA
TTCCGACGGCGATGTCGGCCTGCACGCGCTGGTCGACGCCATTCTGGGCGCACTGGCCGACGGCGACATCGGCTCCCACT
TCCCGCCCTCCGACATGAAGTGGAAGGGCGCCTCCTCCGACCAGTTCCTCAAATACGCCATCGAGCGCGTCGCCGCCCGT
GGCGGCCGCGTCGCCAATCTCGAGGTCACGATGATCTGCGAGCGTCCGAAGATCGGCCCCTTGCGCGACCAGATGCGCGC
GCGCATCGCCGAGATTTCCGGCGTCGACATCTCGCGCATCGCCGTCAAGGCCACCACCAGCGAACGCCTCGGCTTCACCG
GCCGCGAGGAAGGCATCGCGGCGACGGCCAGCGCGACGATCAGGTTGCCATGGGGCGCATGA

Upstream 100 bases:

>100_bases
CACGCAATGTGTGATCCTGCTCGCTTAACATCTTCTCCGGATGTAAGAAGCCGCGTCCATTCACCGCTCCCCATCTCATC
AGCATCGATACCCTGTTCCC

Downstream 100 bases:

>100_bases
ATCCGGGCGGCCCTCAGAGCTAAGATCGAAGCGAACACGCGCAAGGGGACGTTGACTGCTTCGATGAGAAACGAGATCGA
GCTGATCTCTGGACGTCTGA

Product: IspD/ispF bifunctional enzyme

Products: NA

Alternate protein names: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase; 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT; 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase; MECDP-synthase; MECPS [H]

Number of amino acids: Translated: 393; Mature: 392

Protein sequence:

>393_residues
MTTSQRTAAILVAAGRGLRAGAGGPKQYRTIGGRTVIHRALAAFAEHPEVAVVQPVVNPDDIDVFNAAVAGLRHEAPARG
GATRQASVLAGLEALVPHKPEIVLIHDAARPFVTPAVISRAIIAANKTGAAIPVAPVTDTIKEVGASGDITATPERAKLR
IAQTPQTFRFDTILEAHRRAAREGLTEFTDDAAIAEWAGLTVATFEGDVANMKLTTPEDFVREEARLAASLGDIRTGTGY
DVHAFGEGDHVWLCGLRVPHTKGFLAHSDGDVGLHALVDAILGALADGDIGSHFPPSDMKWKGASSDQFLKYAIERVAAR
GGRVANLEVTMICERPKIGPLRDQMRARIAEISGVDISRIAVKATTSERLGFTGREEGIAATASATIRLPWGA

Sequences:

>Translated_393_residues
MTTSQRTAAILVAAGRGLRAGAGGPKQYRTIGGRTVIHRALAAFAEHPEVAVVQPVVNPDDIDVFNAAVAGLRHEAPARG
GATRQASVLAGLEALVPHKPEIVLIHDAARPFVTPAVISRAIIAANKTGAAIPVAPVTDTIKEVGASGDITATPERAKLR
IAQTPQTFRFDTILEAHRRAAREGLTEFTDDAAIAEWAGLTVATFEGDVANMKLTTPEDFVREEARLAASLGDIRTGTGY
DVHAFGEGDHVWLCGLRVPHTKGFLAHSDGDVGLHALVDAILGALADGDIGSHFPPSDMKWKGASSDQFLKYAIERVAAR
GGRVANLEVTMICERPKIGPLRDQMRARIAEISGVDISRIAVKATTSERLGFTGREEGIAATASATIRLPWGA
>Mature_392_residues
TTSQRTAAILVAAGRGLRAGAGGPKQYRTIGGRTVIHRALAAFAEHPEVAVVQPVVNPDDIDVFNAAVAGLRHEAPARGG
ATRQASVLAGLEALVPHKPEIVLIHDAARPFVTPAVISRAIIAANKTGAAIPVAPVTDTIKEVGASGDITATPERAKLRI
AQTPQTFRFDTILEAHRRAAREGLTEFTDDAAIAEWAGLTVATFEGDVANMKLTTPEDFVREEARLAASLGDIRTGTGYD
VHAFGEGDHVWLCGLRVPHTKGFLAHSDGDVGLHALVDAILGALADGDIGSHFPPSDMKWKGASSDQFLKYAIERVAARG
GRVANLEVTMICERPKIGPLRDQMRARIAEISGVDISRIAVKATTSERLGFTGREEGIAATASATIRLPWGA

Specific function: Bifunctional enzyme that catalyzes the formation of 4- diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl- D-erythritol 4-phosphate (MEP) (ispD), and converts 4- diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate into 2-C- methyl-D-erythri

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the ispF family [H]

Homologues:

Organism=Homo sapiens, GI157412259, Length=231, Percent_Identity=30.7359307359307, Blast_Score=102, Evalue=7e-22,
Organism=Homo sapiens, GI157671913, Length=136, Percent_Identity=32.3529411764706, Blast_Score=69, Evalue=8e-12,
Organism=Escherichia coli, GI1789103, Length=154, Percent_Identity=51.9480519480519, Blast_Score=147, Evalue=2e-36,
Organism=Escherichia coli, GI1789104, Length=234, Percent_Identity=35.4700854700855, Blast_Score=100, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001228
- InterPro:   IPR018294
- InterPro:   IPR003526
- InterPro:   IPR020555 [H]

Pfam domain/function: PF01128 IspD; PF02542 YgbB [H]

EC number: =2.7.7.60; =4.6.1.12 [H]

Molecular weight: Translated: 41490; Mature: 41358

Theoretical pI: Translated: 7.17; Mature: 7.17

Prosite motif: PS01295 ISPD ; PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTSQRTAAILVAAGRGLRAGAGGPKQYRTIGGRTVIHRALAAFAEHPEVAVVQPVVNPD
CCCCCCEEEEEEECCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHCCCCEEEEECCCCCC
DIDVFNAAVAGLRHEAPARGGATRQASVLAGLEALVPHKPEIVLIHDAARPFVTPAVISR
CHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHH
AIIAANKTGAAIPVAPVTDTIKEVGASGDITATPERAKLRIAQTPQTFRFDTILEAHRRA
HHHEECCCCCEEECCCHHHHHHHHCCCCCEEECCCHHEEEEECCCCCCHHHHHHHHHHHH
AREGLTEFTDDAAIAEWAGLTVATFEGDVANMKLTTPEDFVREEARLAASLGDIRTGTGY
HHHHHHHHCCCHHHHHHCCCEEEEECCCCCEEEECCHHHHHHHHHHHHHHHCCCCCCCCC
DVHAFGEGDHVWLCGLRVPHTKGFLAHSDGDVGLHALVDAILGALADGDIGSHFPPSDMK
EEEEECCCCEEEEEEEECCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCE
WKGASSDQFLKYAIERVAARGGRVANLEVTMICERPKIGPLRDQMRARIAEISGVDISRI
ECCCCHHHHHHHHHHHHHHCCCEEEEEEEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEE
AVKATTSERLGFTGREEGIAATASATIRLPWGA
EEEECCHHHCCCCCCCCCEEEECEEEEEECCCC
>Mature Secondary Structure 
TTSQRTAAILVAAGRGLRAGAGGPKQYRTIGGRTVIHRALAAFAEHPEVAVVQPVVNPD
CCCCCEEEEEEECCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHCCCCEEEEECCCCCC
DIDVFNAAVAGLRHEAPARGGATRQASVLAGLEALVPHKPEIVLIHDAARPFVTPAVISR
CHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHH
AIIAANKTGAAIPVAPVTDTIKEVGASGDITATPERAKLRIAQTPQTFRFDTILEAHRRA
HHHEECCCCCEEECCCHHHHHHHHCCCCCEEECCCHHEEEEECCCCCCHHHHHHHHHHHH
AREGLTEFTDDAAIAEWAGLTVATFEGDVANMKLTTPEDFVREEARLAASLGDIRTGTGY
HHHHHHHHCCCHHHHHHCCCEEEEECCCCCEEEECCHHHHHHHHHHHHHHHCCCCCCCCC
DVHAFGEGDHVWLCGLRVPHTKGFLAHSDGDVGLHALVDAILGALADGDIGSHFPPSDMK
EEEEECCCCEEEEEEEECCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCE
WKGASSDQFLKYAIERVAARGGRVANLEVTMICERPKIGPLRDQMRARIAEISGVDISRI
ECCCCHHHHHHHHHHHHHHCCCEEEEEEEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEE
AVKATTSERLGFTGREEGIAATASATIRLPWGA
EEEECCHHHCCCCCCCCCEEEECEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA