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The map label for this gene is ntrX [H]

Identifier: 146340797

GI number: 146340797

Start: 4028520

End: 4029890

Strand: Reverse

Name: ntrX [H]

Synonym: BRADO3856

Alternate gene names: 146340797

Gene position: 4029890-4028520 (Counterclockwise)

Preceding gene: 146340798

Following gene: 146340796

Centisome position: 54.04

GC content: 65.65

Gene sequence:

>1371_bases
ATGGCGAGTGATATTCTCATTGTCGACGACGAAGCCGATATCCGGGAGCTGGTTGCCGGCATCCTGGACGATGAAGGCTT
CACGACTCGAACGGCTCGCGACAGCGATTCGGCGCTCGCCGAGATCGCGGGCCGCCGGCCGAACCTCGTGTTCCTCGACA
TCTGGCTGCAAGGCTCCAAGCTCGACGGCCTGCAGCTGCTCGAGCAGATCAAGAAGGACCACCCCGATCTGCCGGTCGTG
ATGATCTCGGGCCACGGCAACATCGAGACCGCGGTCGCTGCGATCAAGCGCGGCGCCTATGACTTCATCGAGAAGCCGTT
CAAGTCTGACCGCCTGATTCTCGTCGCGACGCGCGCGCTGGAGAATTCGCGGCTGAAGCGCGAGGTGCGCGAGCTGAAGC
AGCTGGCGCCGACCGCGAGCACGATGACCGGACGCTCGCCCTCGATGAACCAGCTGCGCCAGACCATCGAACGCGCTGCC
AAGGCCAACAGCCGCATCCTGATCGTCGGACCTCCGGGCTCCGGCAAGGAGCTCGCTGCGCGCACGCTGCACGCGCAGTC
GCCGCGCGCGGAAGGACCGTTCGTCGTCATCAACGCCGCCGCGATCACGCCGGAGCGGATGGAAGAGGAGCTGTTCGGCA
TCGAGCAGTCCAACGGCGAGCAGTCGCGCAAGACTGGCGCGCTCGAGGAGGCGCATGGCGGCACGCTGTTCGTCGACGAG
ATCGCGGACATGCCGCGCGAGACCCAGAACAAGATCCTGCGCGTGCTGGTCGACCAGACCTTCCAGCGGGCGGGCGGCAC
GACGAAAGTGCACGTTGATGTGCGTATCATTTCCTCGACCGCGCGGAACCTGGAGGAGGAGATTGCCAGCGGCCGCTTCC
GCGAGGATCTCTATCACCGCCTCTCGGTGGTTCCGATCCGCGTGCCGCCGCTATCCGAGCGGCGCGAGGACATTCCCGAG
CTGATCGACTACTTCATGGACCAGATCTCGGTCACGACCGGCTTGCCGAAGCGGCAGATCGGCCAGGATGCGATGGCTGT
GCTGCAGTCGCATGTCTGGCCCGGCAACGTCCGCCAGCTTCGCAATAACGTCGAGCGCGTGATGATCCTGGCCGCCGGCG
GTCCGGAGGTGATCATCACCGCGGACATGCTGCCGCCGGACGTCGGCTCGATGGTGCCGGCGATGCCGACCAGCAACAAT
GGCGAGCACATCATGGGCCTGCCGCTTCGCGAGGCGCGCGAGGTGTTCGAGCGCGATTACCTGATCGCGCAAATCAGCCG
ATTCTCCGGCAACATCTCGCGCACCGCCGAATTCGTCGGGATGGAGCGCTCGGCCCTGCATCGCAAGCTGAAGGCGCTCG
GCGTCGGCTGA

Upstream 100 bases:

>100_bases
AGGCGGAGACCGGCGGGGAAACCGCCGGCACAGCTCAAGCGACCAACGATCAACCAAAGATTCAAGCCGCAACCAGCGGC
TGACGAGACAGGCGTGACCC

Downstream 100 bases:

>100_bases
GGTCATCAGTCGGCAAAGTTTGCCGGGTTGGACGAATTTACATTGGAAAATTCGGCTGGCACCGGATATTTACGGGCGAA
TCCCGGTGTTTTGGAACCGG

Product: nitrogen assimilation regulatory protein

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 456; Mature: 455

Protein sequence:

>456_residues
MASDILIVDDEADIRELVAGILDDEGFTTRTARDSDSALAEIAGRRPNLVFLDIWLQGSKLDGLQLLEQIKKDHPDLPVV
MISGHGNIETAVAAIKRGAYDFIEKPFKSDRLILVATRALENSRLKREVRELKQLAPTASTMTGRSPSMNQLRQTIERAA
KANSRILIVGPPGSGKELAARTLHAQSPRAEGPFVVINAAAITPERMEEELFGIEQSNGEQSRKTGALEEAHGGTLFVDE
IADMPRETQNKILRVLVDQTFQRAGGTTKVHVDVRIISSTARNLEEEIASGRFREDLYHRLSVVPIRVPPLSERREDIPE
LIDYFMDQISVTTGLPKRQIGQDAMAVLQSHVWPGNVRQLRNNVERVMILAAGGPEVIITADMLPPDVGSMVPAMPTSNN
GEHIMGLPLREAREVFERDYLIAQISRFSGNISRTAEFVGMERSALHRKLKALGVG

Sequences:

>Translated_456_residues
MASDILIVDDEADIRELVAGILDDEGFTTRTARDSDSALAEIAGRRPNLVFLDIWLQGSKLDGLQLLEQIKKDHPDLPVV
MISGHGNIETAVAAIKRGAYDFIEKPFKSDRLILVATRALENSRLKREVRELKQLAPTASTMTGRSPSMNQLRQTIERAA
KANSRILIVGPPGSGKELAARTLHAQSPRAEGPFVVINAAAITPERMEEELFGIEQSNGEQSRKTGALEEAHGGTLFVDE
IADMPRETQNKILRVLVDQTFQRAGGTTKVHVDVRIISSTARNLEEEIASGRFREDLYHRLSVVPIRVPPLSERREDIPE
LIDYFMDQISVTTGLPKRQIGQDAMAVLQSHVWPGNVRQLRNNVERVMILAAGGPEVIITADMLPPDVGSMVPAMPTSNN
GEHIMGLPLREAREVFERDYLIAQISRFSGNISRTAEFVGMERSALHRKLKALGVG
>Mature_455_residues
ASDILIVDDEADIRELVAGILDDEGFTTRTARDSDSALAEIAGRRPNLVFLDIWLQGSKLDGLQLLEQIKKDHPDLPVVM
ISGHGNIETAVAAIKRGAYDFIEKPFKSDRLILVATRALENSRLKREVRELKQLAPTASTMTGRSPSMNQLRQTIERAAK
ANSRILIVGPPGSGKELAARTLHAQSPRAEGPFVVINAAAITPERMEEELFGIEQSNGEQSRKTGALEEAHGGTLFVDEI
ADMPRETQNKILRVLVDQTFQRAGGTTKVHVDVRIISSTARNLEEEIASGRFREDLYHRLSVVPIRVPPLSERREDIPEL
IDYFMDQISVTTGLPKRQIGQDAMAVLQSHVWPGNVRQLRNNVERVMILAAGGPEVIITADMLPPDVGSMVPAMPTSNNG
EHIMGLPLREAREVFERDYLIAQISRFSGNISRTAEFVGMERSALHRKLKALGVG

Specific function: Member of the two-component regulatory system ntrY/ntrX involved in the activation of nitrogen assimilatory genes such as glnA. NtrX is probably phosphorylated by ntrY and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=470, Percent_Identity=36.8085106382979, Blast_Score=308, Evalue=4e-85,
Organism=Escherichia coli, GI1788550, Length=461, Percent_Identity=36.0086767895879, Blast_Score=294, Evalue=8e-81,
Organism=Escherichia coli, GI1790437, Length=451, Percent_Identity=35.4767184035477, Blast_Score=258, Evalue=6e-70,
Organism=Escherichia coli, GI1788905, Length=450, Percent_Identity=34.4444444444444, Blast_Score=246, Evalue=2e-66,
Organism=Escherichia coli, GI87082152, Length=325, Percent_Identity=38.7692307692308, Blast_Score=210, Evalue=2e-55,
Organism=Escherichia coli, GI1786524, Length=328, Percent_Identity=37.1951219512195, Blast_Score=200, Evalue=2e-52,
Organism=Escherichia coli, GI1789233, Length=255, Percent_Identity=40, Blast_Score=195, Evalue=5e-51,
Organism=Escherichia coli, GI1787583, Length=316, Percent_Identity=35.7594936708861, Blast_Score=191, Evalue=9e-50,
Organism=Escherichia coli, GI87082117, Length=232, Percent_Identity=41.8103448275862, Blast_Score=183, Evalue=2e-47,
Organism=Escherichia coli, GI1789087, Length=316, Percent_Identity=35.126582278481, Blast_Score=182, Evalue=3e-47,
Organism=Escherichia coli, GI87081872, Length=328, Percent_Identity=32.9268292682927, Blast_Score=158, Evalue=6e-40,
Organism=Escherichia coli, GI1789828, Length=263, Percent_Identity=31.9391634980989, Blast_Score=125, Evalue=7e-30,
Organism=Escherichia coli, GI87081858, Length=332, Percent_Identity=27.710843373494, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1786599, Length=115, Percent_Identity=39.1304347826087, Blast_Score=82, Evalue=9e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 50360; Mature: 50229

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASDILIVDDEADIRELVAGILDDEGFTTRTARDSDSALAEIAGRRPNLVFLDIWLQGSK
CCCCEEEECCCHHHHHHHHHHHCCCCCEECCCCCCHHHHHHHHCCCCCEEEEEEEEECCC
LDGLQLLEQIKKDHPDLPVVMISGHGNIETAVAAIKRGAYDFIEKPFKSDRLILVATRAL
CCHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHCCHHHHHCCCCCCCEEEEEEHHH
ENSRLKREVRELKQLAPTASTMTGRSPSMNQLRQTIERAAKANSRILIVGPPGSGKELAA
HHHHHHHHHHHHHHHCCCHHHCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHH
RTLHAQSPRAEGPFVVINAAAITPERMEEELFGIEQSNGEQSRKTGALEEAHGGTLFVDE
HHHHCCCCCCCCCEEEEEEEECCHHHHHHHHHCCCCCCCCHHHHCCCHHHCCCCEEEHHH
IADMPRETQNKILRVLVDQTFQRAGGTTKVHVDVRIISSTARNLEEEIASGRFREDLYHR
HHCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECHHHHHHHHHHHCCHHHHHHHHH
LSVVPIRVPPLSERREDIPELIDYFMDQISVTTGLPKRQIGQDAMAVLQSHVWPGNVRQL
HEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCHHHHHHHHHCCCCCHHHHH
RNNVERVMILAAGGPEVIITADMLPPDVGSMVPAMPTSNNGEHIMGLPLREAREVFERDY
HCCCCEEEEEECCCCCEEEECCCCCCCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHH
LIAQISRFSGNISRTAEFVGMERSALHRKLKALGVG
HHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ASDILIVDDEADIRELVAGILDDEGFTTRTARDSDSALAEIAGRRPNLVFLDIWLQGSK
CCCEEEECCCHHHHHHHHHHHCCCCCEECCCCCCHHHHHHHHCCCCCEEEEEEEEECCC
LDGLQLLEQIKKDHPDLPVVMISGHGNIETAVAAIKRGAYDFIEKPFKSDRLILVATRAL
CCHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHCCHHHHHCCCCCCCEEEEEEHHH
ENSRLKREVRELKQLAPTASTMTGRSPSMNQLRQTIERAAKANSRILIVGPPGSGKELAA
HHHHHHHHHHHHHHHCCCHHHCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHH
RTLHAQSPRAEGPFVVINAAAITPERMEEELFGIEQSNGEQSRKTGALEEAHGGTLFVDE
HHHHCCCCCCCCCEEEEEEEECCHHHHHHHHHCCCCCCCCHHHHCCCHHHCCCCEEEHHH
IADMPRETQNKILRVLVDQTFQRAGGTTKVHVDVRIISSTARNLEEEIASGRFREDLYHR
HHCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECHHHHHHHHHHHCCHHHHHHHHH
LSVVPIRVPPLSERREDIPELIDYFMDQISVTTGLPKRQIGQDAMAVLQSHVWPGNVRQL
HEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCHHHHHHHHHCCCCCHHHHH
RNNVERVMILAAGGPEVIITADMLPPDVGSMVPAMPTSNNGEHIMGLPLREAREVFERDY
HCCCCEEEEEECCCCCEEEECCCCCCCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHH
LIAQISRFSGNISRTAEFVGMERSALHRKLKALGVG
HHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1661370 [H]