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The map label for this gene is rimO

Identifier: 146340736

GI number: 146340736

Start: 3960751

End: 3962088

Strand: Reverse

Name: rimO

Synonym: BRADO3790

Alternate gene names: 146340736

Gene position: 3962088-3960751 (Counterclockwise)

Preceding gene: 146340741

Following gene: 146340734

Centisome position: 53.14

GC content: 66.37

Gene sequence:

>1338_bases
ATGCAGCAGGGTTCCGCGCCCAATTCCGCGCCCAAGATCAGCTTTGTTTCGCTCGGTTGTCCGAAGGCGTTGGTCGATTC
CGAGCGCATCATCACGCGGCTTCGCGCCGAGGGCTACGAGCTCGCCCGCAAGCATGACGGCGCCGACGTCGTCATCGTCA
ACACCTGCGGCTTCCTCGACAGCGCCAAGCAGGAATCGCTGGCGGCGATCGGCTCGGCCATGGCCGAGAACGGCAAGGTG
ATCGTCACCGGCTGCATGGGCGCCGAGCCCGAGCAGATCGAGCAGGCCTATCCCGGCGTGCTGTCGATCACCGGTCCCCA
GCAGTACGAAAGCGTCCTGGACGCCGTTCACCGCGCGTCGCCGCCCGTGCACAACCCTCATCTGGATCTGGTTCCTCCCC
AGGGCATCAAGCTGACGCCCCGGCACTACGCCTATCTGAAGATTTCCGAAGGCTGCAACAACAGGTGCAGCTTCTGCATC
ATCCCGAAGCTGCGCGGTGATCTGGTCTCGCGGCCGGCTGCGGATGTGCTGCGCGAGGCTGAGCGGCTGGTCGCAGCCGG
CGTCAAGGAGCTGCTGGTCATCTCGCAGGACACGTCCGCCTATGGCCTCGACATCAAATACGCCGAGAGCCCGTGGAAGG
ACCGCAGCGTCCGGGCCCGTTTCCTCGACTTGGCCCGTGAGCTCGGCGAGCTCGGCGCCTGGGTGCGGCTGCACTATGTC
TACCCCTACCCGCATGTCGACGAGGTCATCGGCCTCATGACCGACGGCAAGGTGCTGCCTTATCTCGACATCCCGTTCCA
GCATGCCAGCCCCAACGTGCTGAAGGCCATGCGGCGGCCGGCGGCGCAGGACAAGACGCTGGACCGCATCAAGGCCTGGC
GCGGGGCCTGCCCGGATCTGGCGCTGCGCTCGACCTTCATCGTCGGCTTCCCCGGCGAGACCGACGAGGATTTCGCCTAT
CTGCTGGACTGGCTGGACGAGGCGGAGATCGACCGGCTCGGCTGCTTCAAATATGAGCCGGTGGCCGGCGCGACGTCGAA
CGCGCTGCCCGGCCAGGTGCCCGACGAGGTCAAGCAGGAGCGCTGGAACGCCTTGATGGCGCGCCAGCAGAAGATCTCCG
CGCGGCGGCTGAAGCGGAAAGTCGGAACCCGGCAGCAGATCATCATCGACGAGGTCGGTCCGACCGTCGCCAAGGGGCGT
TCAAAGGCTGATGCACCGGAGATCGACGGGGCCGTCTATCTCTCCAGCCGGAGGCCGCTGCGGGCCGGCGAGATCGTCAC
GGCCAAGATCGAGCGCGCCGACGAATACGACCTGCACGGCACTGTGGCGGGGTTCTGA

Upstream 100 bases:

>100_bases
TGTCCGGACCGAATCCATAGCATGGACGCCACCGGCTTAAAGCCTTGTCTTTCTGAGCAATTCGGACTAGCTGACGCGTA
TCCCATCAGGCAGTTCCAAG

Downstream 100 bases:

>100_bases
TCCCGGCTCATGCACCGCTCGCCGACGAGCCCGCTCTGACGTTCCACAGCTTGATGAGCCGTGTCGCCTCGTCGAGCGTG
AGCTCGAGATATTTGGCCTT

Product: ribosomal protein S12 methylthiotransferase

Products: NA

Alternate protein names: S12 MTTase; S12 methylthiotransferase; Ribosome maturation factor RimO

Number of amino acids: Translated: 445; Mature: 445

Protein sequence:

>445_residues
MQQGSAPNSAPKISFVSLGCPKALVDSERIITRLRAEGYELARKHDGADVVIVNTCGFLDSAKQESLAAIGSAMAENGKV
IVTGCMGAEPEQIEQAYPGVLSITGPQQYESVLDAVHRASPPVHNPHLDLVPPQGIKLTPRHYAYLKISEGCNNRCSFCI
IPKLRGDLVSRPAADVLREAERLVAAGVKELLVISQDTSAYGLDIKYAESPWKDRSVRARFLDLARELGELGAWVRLHYV
YPYPHVDEVIGLMTDGKVLPYLDIPFQHASPNVLKAMRRPAAQDKTLDRIKAWRGACPDLALRSTFIVGFPGETDEDFAY
LLDWLDEAEIDRLGCFKYEPVAGATSNALPGQVPDEVKQERWNALMARQQKISARRLKRKVGTRQQIIIDEVGPTVAKGR
SKADAPEIDGAVYLSSRRPLRAGEIVTAKIERADEYDLHGTVAGF

Sequences:

>Translated_445_residues
MQQGSAPNSAPKISFVSLGCPKALVDSERIITRLRAEGYELARKHDGADVVIVNTCGFLDSAKQESLAAIGSAMAENGKV
IVTGCMGAEPEQIEQAYPGVLSITGPQQYESVLDAVHRASPPVHNPHLDLVPPQGIKLTPRHYAYLKISEGCNNRCSFCI
IPKLRGDLVSRPAADVLREAERLVAAGVKELLVISQDTSAYGLDIKYAESPWKDRSVRARFLDLARELGELGAWVRLHYV
YPYPHVDEVIGLMTDGKVLPYLDIPFQHASPNVLKAMRRPAAQDKTLDRIKAWRGACPDLALRSTFIVGFPGETDEDFAY
LLDWLDEAEIDRLGCFKYEPVAGATSNALPGQVPDEVKQERWNALMARQQKISARRLKRKVGTRQQIIIDEVGPTVAKGR
SKADAPEIDGAVYLSSRRPLRAGEIVTAKIERADEYDLHGTVAGF
>Mature_445_residues
MQQGSAPNSAPKISFVSLGCPKALVDSERIITRLRAEGYELARKHDGADVVIVNTCGFLDSAKQESLAAIGSAMAENGKV
IVTGCMGAEPEQIEQAYPGVLSITGPQQYESVLDAVHRASPPVHNPHLDLVPPQGIKLTPRHYAYLKISEGCNNRCSFCI
IPKLRGDLVSRPAADVLREAERLVAAGVKELLVISQDTSAYGLDIKYAESPWKDRSVRARFLDLARELGELGAWVRLHYV
YPYPHVDEVIGLMTDGKVLPYLDIPFQHASPNVLKAMRRPAAQDKTLDRIKAWRGACPDLALRSTFIVGFPGETDEDFAY
LLDWLDEAEIDRLGCFKYEPVAGATSNALPGQVPDEVKQERWNALMARQQKISARRLKRKVGTRQQIIIDEVGPTVAKGR
SKADAPEIDGAVYLSSRRPLRAGEIVTAKIERADEYDLHGTVAGF

Specific function: Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI93277076, Length=399, Percent_Identity=28.5714285714286, Blast_Score=135, Evalue=8e-32,
Organism=Homo sapiens, GI28872784, Length=435, Percent_Identity=25.2873563218391, Blast_Score=127, Evalue=3e-29,
Organism=Homo sapiens, GI28872782, Length=435, Percent_Identity=25.2873563218391, Blast_Score=126, Evalue=4e-29,
Organism=Escherichia coli, GI1787057, Length=432, Percent_Identity=65.2777777777778, Blast_Score=588, Evalue=1e-169,
Organism=Escherichia coli, GI1786882, Length=399, Percent_Identity=28.3208020050125, Blast_Score=152, Evalue=3e-38,
Organism=Caenorhabditis elegans, GI17553146, Length=474, Percent_Identity=24.4725738396624, Blast_Score=116, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI71996771, Length=256, Percent_Identity=29.6875, Blast_Score=99, Evalue=5e-21,
Organism=Drosophila melanogaster, GI21356207, Length=477, Percent_Identity=25.3668763102725, Blast_Score=140, Evalue=2e-33,
Organism=Drosophila melanogaster, GI19922432, Length=323, Percent_Identity=27.2445820433437, Blast_Score=107, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RIMO_BRASO (A4YUI3)

Other databases:

- EMBL:   CU234118
- RefSeq:   YP_001205784.1
- ProteinModelPortal:   A4YUI3
- STRING:   A4YUI3
- GeneID:   5117622
- GenomeReviews:   CU234118_GR
- KEGG:   bra:BRADO3790
- eggNOG:   COG0621
- HOGENOM:   HBG457663
- OMA:   KADAPEI
- ProtClustDB:   PRK14862
- BioCyc:   BSP376:BRADO3790-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01865
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR012340
- InterPro:   IPR007197
- InterPro:   IPR005840
- InterPro:   IPR002792
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01125
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 48932; Mature: 48932

Theoretical pI: Translated: 6.99; Mature: 6.99

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQGSAPNSAPKISFVSLGCPKALVDSERIITRLRAEGYELARKHDGADVVIVNTCGFLD
CCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCC
SAKQESLAAIGSAMAENGKVIVTGCMGAEPEQIEQAYPGVLSITGPQQYESVLDAVHRAS
HHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHCCCEEEECCHHHHHHHHHHHHHCC
PPVHNPHLDLVPPQGIKLTPRHYAYLKISEGCNNRCSFCIIPKLRGDLVSRPAADVLREA
CCCCCCCCCCCCCCCCEECCCEEEEEEECCCCCCCCCEEEECCHHHHHHCCCHHHHHHHH
ERLVAAGVKELLVISQDTSAYGLDIKYAESPWKDRSVRARFLDLARELGELGAWVRLHYV
HHHHHHCHHHEEEEECCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCEEEEEEE
YPYPHVDEVIGLMTDGKVLPYLDIPFQHASPNVLKAMRRPAAQDKTLDRIKAWRGACPDL
CCCCCHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHCCCCCH
ALRSTFIVGFPGETDEDFAYLLDWLDEAEIDRLGCFKYEPVAGATSNALPGQVPDEVKQE
HCCCEEEEECCCCCCHHHHHHHHHHCHHHHHHHCCEEECCCCCCCCCCCCCCCCHHHHHH
RWNALMARQQKISARRLKRKVGTRQQIIIDEVGPTVAKGRSKADAPEIDGAVYLSSRRPL
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHCCCCCCCCCCCCCEEEECCCCCC
RAGEIVTAKIERADEYDLHGTVAGF
CCCCEEEEEECCCCCCCCCEECCCC
>Mature Secondary Structure
MQQGSAPNSAPKISFVSLGCPKALVDSERIITRLRAEGYELARKHDGADVVIVNTCGFLD
CCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCC
SAKQESLAAIGSAMAENGKVIVTGCMGAEPEQIEQAYPGVLSITGPQQYESVLDAVHRAS
HHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHCCCEEEECCHHHHHHHHHHHHHCC
PPVHNPHLDLVPPQGIKLTPRHYAYLKISEGCNNRCSFCIIPKLRGDLVSRPAADVLREA
CCCCCCCCCCCCCCCCEECCCEEEEEEECCCCCCCCCEEEECCHHHHHHCCCHHHHHHHH
ERLVAAGVKELLVISQDTSAYGLDIKYAESPWKDRSVRARFLDLARELGELGAWVRLHYV
HHHHHHCHHHEEEEECCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCEEEEEEE
YPYPHVDEVIGLMTDGKVLPYLDIPFQHASPNVLKAMRRPAAQDKTLDRIKAWRGACPDL
CCCCCHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHCCCCCH
ALRSTFIVGFPGETDEDFAYLLDWLDEAEIDRLGCFKYEPVAGATSNALPGQVPDEVKQE
HCCCEEEEECCCCCCHHHHHHHHHHCHHHHHHHCCEEECCCCCCCCCCCCCCCCHHHHHH
RWNALMARQQKISARRLKRKVGTRQQIIIDEVGPTVAKGRSKADAPEIDGAVYLSSRRPL
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHCCCCCCCCCCCCCEEEECCCCCC
RAGEIVTAKIERADEYDLHGTVAGF
CCCCEEEEEECCCCCCCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA