Click here to switch to the map view.

The map label for this gene is lptD [H]

Identifier: 146340302

GI number: 146340302

Start: 3520708

End: 3523212

Strand: Reverse

Name: lptD [H]

Synonym: BRADO3325

Alternate gene names: 146340302

Gene position: 3523212-3520708 (Counterclockwise)

Preceding gene: 146340303

Following gene: 146340301

Centisome position: 47.25

GC content: 63.87

Gene sequence:

>2505_bases
GTGTCGTTCGTTGCCGTCCGTCAGACCGCGCTCGCCGCGCTCCGGCTGCATGCCGTTGCGCGTGTTGCGTGCGCGCGCGC
CCGTTGCGCCGGGTCTGCCGTACTTGCAGGCGCGTTCGCGCTGACGCTCGGCCTCGTGCTCGCCACCACCGTGGACGCGT
TGATCTCGCCTGCCGCCGCCCAGAGCTTCACCTACAATCCGCGGCCGCCGAAGGCACCGCCGCCCCGCGTCGCCAACGAC
AACCAGATGCTGGTTCAGGCGACAGAGGTCGATTACGACTACAACAACTCGCGCGTGTCGGCGGTCGGCAACGTGCAACT
GTTCTACAACGGCACCTCGGTCGAAGCCGATCGGGTGATCTACGACCAGAAGACCAAGCGCCTGCATGCGGAAGGCAACA
TCCGCATGACCGACGCCGACGGCAAGATCACCTACGCCAACAGCCTCGACCTCTCCGACGATTATCGCGACGGCTTCGTC
GATTCGCTGCGCGTCGAGACCGAGGACCAGACGCGGATGGCCGCGACCCGCGCCGACCGCTCCAAGGGCAATTACACCGT
CTTCGAGAACGGTGTTTATACGGCCTGCGCGCCGTGCAAGGACGATCCGAAGAAGCCGCCGCTGTGGCAGGTCAAGGGTG
CGCGCATCATCCACGACCAGACCGAGAAGATGCTGTATTTCGAGAACGCGCAGCTCGAATTCTTCGGCGTTCCGCTCGCC
TATATCCCCTACTTCTCGACGCCCGATCCGACGGTCAAGCGCAAGAGCGGCTTCCTGATGCCGGGCGTCACCCAGACCGC
CGGCTACGGCTTCGGCGTCGACATCCCCTATTACTGGGCGATCGCGCCGGATTACGACGCGACCTTCACGCCGCGCATCA
CCTCCAAGCAGGGCGTGCTGGTGCAGACCGAATTCCGCCAGCGCCTCGAGAATGGCGGCTATCAGGTCCGCCTCTACGGC
ATCGACCAGTTGAACCGCAACGCGTTCGCCGGCCAGCCCGGCGACCGCCAGTTCCGCGGCGGCATCGACACCAAGGGCCA
GTTCGCGCTCAACGACAAATGGGTGTTCGGCTGGGACGGCATCCTGCTGTCCGACTATACGTTCCTGTCGGACTACCGGC
TGGCGCAATATCGTGACCCGTTCAACTCGTTCATGAGCCTGCCGACGGAGGCGATCTCGCAGCTGTTTCTGACCGGTGTC
GGCAGCCGCAGTTTCTTCGATGTCCGGGCGATGCACTATCTGAGCTTTTCGGGCAATCAGCAGACGGTGCCGGTGGTCTA
TCCGGTGCTGGATTACTCGAACGTGCTGAACTACCCGGTGTTCGGCGGCGAGTTCAGCTACAAGACGAACTTTGTCAATC
TCACCCGCGAACAGGCGGTGTTCGATCCGATCACGACGACGGCCAACACCAACGGCCTCTGCACGACCACATCGGCTGAT
CCGCTCGCGCGGTTGCCGACGCAGTGCCTCTTGCGCGCGATGCCCGGCACCTACACCCGCCTGACCGCACAGGCGGAGTG
GCGGAAGTCGTTCACAGATCCCTTTGGCCAGATCTGGACGCCATTCGCGATCCTGCGCGCCGACGCGATCAACGCCAACG
TGACGGCGCAACCAGGCGTCGGCAACTTCCTGCCGACCGGCGATACCCAGGCGCTGCGGTTGATGCCGACCGTCGGCTTG
GAATACCGCTATCCGTTCATCAACGTTCAGCCGTGGGGAACGACGACGATCGAGCCGATCGCCCAGGTCATCATCCGCCC
GAACGAGACCTATGCCGGCCGGCTGCCGAACGAGGACGCGCAGAGCATGGTGTTCGACGCCAGCAATCTGTTCAGCGTCG
ACAAGTTCTCCGGCTACGACCGCATCGAGGGCGGCGGCCGCGCCAATGTCGGCGTTCAGGCCACCACGCAGTTCGATCGG
GGCGGCACCATCAATGTGCTGTTCGGTCAGTCCTATCACCTGTTCGGCCTGAACTCCTTCGCCGTCCAGGACATGAACAA
CACCGGCATCGGCTCCGGCCTGGACAAGACCGCGTCCGATTACGTCACCCGGGTCGACTACTCGCCGAACCGCACCTACA
CGTTCAGCATGCGCAGCCGGTTCGATCAGCAGACGCTGAACGTCCAGCGCTTCGAGGCCGAGGCCAAGGCGAATTTCGAC
CGCTGGTCGGTCGCCCTTACCTACGGCAACTATGCCGCCGACCCGGCCATCGGCTACCTGAACCGCCGTGAGGGCATCCT
CGGCAGCGGCTCGGTGAAGGTTGCGCCGAACTGGGTGGTGACGGGTGCGGCGCGCTGGGATCTCGCGGCCAACAAGATCA
ACCAGTATGTGATCGGCGCGGGCTACGTGGACGATTGTTTCGTGCTGGCCGCCAACTACGTAACGTCCTATAATTACACG
ACAGGCGTCACCACCCCCGTTCTGAGCCATGCCTACATGCTGCAGATCGGACTTCGGACGCTGGCCAATTCGTCGTCGAC
GGGAGCCGGAGGAGGCATCCAGTAG

Upstream 100 bases:

>100_bases
GCAAGGCTGCGTTGATGGATCCGATCGCTGCGGCGTGGCTGCCGGTGTTCGTCGGCGGCCTCACCGGTTTTATGGCTTTG
CTCTACCAGGAGGACGGCTA

Downstream 100 bases:

>100_bases
CGCGTGCCGGTCTTGCCGGCGTTGCCCTGGCTCCGGCGGCCGGACTCGAGAGGCCTCGCGTTAACCTGTTGCGGTTCCGC
CCAAAGCGGTTTAGCCCGAT

Product: putative organic solvent tolerance protein OstA (ImpA)

Products: NA

Alternate protein names: Organic solvent tolerance protein [H]

Number of amino acids: Translated: 834; Mature: 833

Protein sequence:

>834_residues
MSFVAVRQTALAALRLHAVARVACARARCAGSAVLAGAFALTLGLVLATTVDALISPAAAQSFTYNPRPPKAPPPRVAND
NQMLVQATEVDYDYNNSRVSAVGNVQLFYNGTSVEADRVIYDQKTKRLHAEGNIRMTDADGKITYANSLDLSDDYRDGFV
DSLRVETEDQTRMAATRADRSKGNYTVFENGVYTACAPCKDDPKKPPLWQVKGARIIHDQTEKMLYFENAQLEFFGVPLA
YIPYFSTPDPTVKRKSGFLMPGVTQTAGYGFGVDIPYYWAIAPDYDATFTPRITSKQGVLVQTEFRQRLENGGYQVRLYG
IDQLNRNAFAGQPGDRQFRGGIDTKGQFALNDKWVFGWDGILLSDYTFLSDYRLAQYRDPFNSFMSLPTEAISQLFLTGV
GSRSFFDVRAMHYLSFSGNQQTVPVVYPVLDYSNVLNYPVFGGEFSYKTNFVNLTREQAVFDPITTTANTNGLCTTTSAD
PLARLPTQCLLRAMPGTYTRLTAQAEWRKSFTDPFGQIWTPFAILRADAINANVTAQPGVGNFLPTGDTQALRLMPTVGL
EYRYPFINVQPWGTTTIEPIAQVIIRPNETYAGRLPNEDAQSMVFDASNLFSVDKFSGYDRIEGGGRANVGVQATTQFDR
GGTINVLFGQSYHLFGLNSFAVQDMNNTGIGSGLDKTASDYVTRVDYSPNRTYTFSMRSRFDQQTLNVQRFEAEAKANFD
RWSVALTYGNYAADPAIGYLNRREGILGSGSVKVAPNWVVTGAARWDLAANKINQYVIGAGYVDDCFVLAANYVTSYNYT
TGVTTPVLSHAYMLQIGLRTLANSSSTGAGGGIQ

Sequences:

>Translated_834_residues
MSFVAVRQTALAALRLHAVARVACARARCAGSAVLAGAFALTLGLVLATTVDALISPAAAQSFTYNPRPPKAPPPRVAND
NQMLVQATEVDYDYNNSRVSAVGNVQLFYNGTSVEADRVIYDQKTKRLHAEGNIRMTDADGKITYANSLDLSDDYRDGFV
DSLRVETEDQTRMAATRADRSKGNYTVFENGVYTACAPCKDDPKKPPLWQVKGARIIHDQTEKMLYFENAQLEFFGVPLA
YIPYFSTPDPTVKRKSGFLMPGVTQTAGYGFGVDIPYYWAIAPDYDATFTPRITSKQGVLVQTEFRQRLENGGYQVRLYG
IDQLNRNAFAGQPGDRQFRGGIDTKGQFALNDKWVFGWDGILLSDYTFLSDYRLAQYRDPFNSFMSLPTEAISQLFLTGV
GSRSFFDVRAMHYLSFSGNQQTVPVVYPVLDYSNVLNYPVFGGEFSYKTNFVNLTREQAVFDPITTTANTNGLCTTTSAD
PLARLPTQCLLRAMPGTYTRLTAQAEWRKSFTDPFGQIWTPFAILRADAINANVTAQPGVGNFLPTGDTQALRLMPTVGL
EYRYPFINVQPWGTTTIEPIAQVIIRPNETYAGRLPNEDAQSMVFDASNLFSVDKFSGYDRIEGGGRANVGVQATTQFDR
GGTINVLFGQSYHLFGLNSFAVQDMNNTGIGSGLDKTASDYVTRVDYSPNRTYTFSMRSRFDQQTLNVQRFEAEAKANFD
RWSVALTYGNYAADPAIGYLNRREGILGSGSVKVAPNWVVTGAARWDLAANKINQYVIGAGYVDDCFVLAANYVTSYNYT
TGVTTPVLSHAYMLQIGLRTLANSSSTGAGGGIQ
>Mature_833_residues
SFVAVRQTALAALRLHAVARVACARARCAGSAVLAGAFALTLGLVLATTVDALISPAAAQSFTYNPRPPKAPPPRVANDN
QMLVQATEVDYDYNNSRVSAVGNVQLFYNGTSVEADRVIYDQKTKRLHAEGNIRMTDADGKITYANSLDLSDDYRDGFVD
SLRVETEDQTRMAATRADRSKGNYTVFENGVYTACAPCKDDPKKPPLWQVKGARIIHDQTEKMLYFENAQLEFFGVPLAY
IPYFSTPDPTVKRKSGFLMPGVTQTAGYGFGVDIPYYWAIAPDYDATFTPRITSKQGVLVQTEFRQRLENGGYQVRLYGI
DQLNRNAFAGQPGDRQFRGGIDTKGQFALNDKWVFGWDGILLSDYTFLSDYRLAQYRDPFNSFMSLPTEAISQLFLTGVG
SRSFFDVRAMHYLSFSGNQQTVPVVYPVLDYSNVLNYPVFGGEFSYKTNFVNLTREQAVFDPITTTANTNGLCTTTSADP
LARLPTQCLLRAMPGTYTRLTAQAEWRKSFTDPFGQIWTPFAILRADAINANVTAQPGVGNFLPTGDTQALRLMPTVGLE
YRYPFINVQPWGTTTIEPIAQVIIRPNETYAGRLPNEDAQSMVFDASNLFSVDKFSGYDRIEGGGRANVGVQATTQFDRG
GTINVLFGQSYHLFGLNSFAVQDMNNTGIGSGLDKTASDYVTRVDYSPNRTYTFSMRSRFDQQTLNVQRFEAEAKANFDR
WSVALTYGNYAADPAIGYLNRREGILGSGSVKVAPNWVVTGAARWDLAANKINQYVIGAGYVDDCFVLAANYVTSYNYTT
GVTTPVLSHAYMLQIGLRTLANSSSTGAGGGIQ

Specific function: Together with lptE, is involved in the assembly of LPS in the outer leaflet of the outer membrane. Determines N-hexane tolerance and is involved in outer membrane permeability. Essential for envelope biogenesis [H]

COG id: COG1452

COG function: function code M; Organic solvent tolerance protein OstA

Gene ontology:

Cell location: Cell outer membrane [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lptD family [H]

Homologues:

Organism=Escherichia coli, GI1786239, Length=799, Percent_Identity=24.0300375469337, Blast_Score=114, Evalue=3e-26,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020889
- InterPro:   IPR005653
- InterPro:   IPR007543 [H]

Pfam domain/function: PF03968 OstA; PF04453 OstA_C [H]

EC number: NA

Molecular weight: Translated: 91957; Mature: 91826

Theoretical pI: Translated: 7.07; Mature: 7.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFVAVRQTALAALRLHAVARVACARARCAGSAVLAGAFALTLGLVLATTVDALISPAAA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHH
QSFTYNPRPPKAPPPRVANDNQMLVQATEVDYDYNNSRVSAVGNVQLFYNGTSVEADRVI
CCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCEEEEEEEEEEEECCCCCCCCCEE
YDQKTKRLHAEGNIRMTDADGKITYANSLDLSDDYRDGFVDSLRVETEDQTRMAATRADR
ECCHHHEEECCCCEEEECCCCEEEEECCCCCCCCHHCCCCHHEEECCCCHHHHHHHHHHC
SKGNYTVFENGVYTACAPCKDDPKKPPLWQVKGARIIHDQTEKMLYFENAQLEFFGVPLA
CCCCEEEEECCEEEEECCCCCCCCCCCEEEECCCEEEECCCCEEEEEECCEEEEEECCHH
YIPYFSTPDPTVKRKSGFLMPGVTQTAGYGFGVDIPYYWAIAPDYDATFTPRITSKQGVL
HCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCEEEEECCCCCCEECCCCCCCCCEE
VQTEFRQRLENGGYQVRLYGIDQLNRNAFAGQPGDRQFRGGIDTKGQFALNDKWVFGWDG
EEHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCHHCCCCCCCCEEEECCCEEEEECC
ILLSDYTFLSDYRLAQYRDPFNSFMSLPTEAISQLFLTGVGSRSFFDVRAMHYLSFSGNQ
EEEECEEEHHCCHHHHHCCHHHHHHCCCHHHHHHHHHHCCCCCCEEEEEEEEEEEECCCC
QTVPVVYPVLDYSNVLNYPVFGGEFSYKTNFVNLTREQAVFDPITTTANTNGLCTTTSAD
EEEEEEEEECCCHHHHCCCEECCCCCEEECEEEECHHHHHCCCCCCCCCCCCEEEECCCC
PLARLPTQCLLRAMPGTYTRLTAQAEWRKSFTDPFGQIWTPFAILRADAINANVTAQPGV
HHHHCCHHHHHHCCCCCCEEEEECHHHHHHHCCHHHHHHCCHHHEEECCCCCCEEECCCC
GNFLPTGDTQALRLMPTVGLEYRYPFINVQPWGTTTIEPIAQVIIRPNETYAGRLPNEDA
CCCCCCCCCCEEEEEECCCCEEECCEEEEECCCCCHHHHHHHHEECCCCCEECCCCCCCH
QSMVFDASNLFSVDKFSGYDRIEGGGRANVGVQATTQFDRGGTINVLFGQSYHLFGLNSF
HHEEEECCCCEEEHHCCCCCCCCCCCCCCCCEEEEEEECCCCEEEEEECCEEEEEECCCE
AVQDMNNTGIGSGLDKTASDYVTRVDYSPNRTYTFSMRSRFDQQTLNVQRFEAEAKANFD
EEECCCCCCCCCCCCCCHHHHEEEEECCCCCEEEEHHHHCCCHHHCCHHEEEHHHHCCCC
RWSVALTYGNYAADPAIGYLNRREGILGSGSVKVAPNWVVTGAARWDLAANKINQYVIGA
EEEEEEEECCCCCCCHHHHHHCCCCCCCCCCEEECCCEEEECCCHHHHHHHCCCEEEEEC
GYVDDCFVLAANYVTSYNYTTGVTTPVLSHAYMLQIGLRTLANSSSTGAGGGIQ
CCHHHHHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
SFVAVRQTALAALRLHAVARVACARARCAGSAVLAGAFALTLGLVLATTVDALISPAAA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHH
QSFTYNPRPPKAPPPRVANDNQMLVQATEVDYDYNNSRVSAVGNVQLFYNGTSVEADRVI
CCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCEEEEEEEEEEEECCCCCCCCCEE
YDQKTKRLHAEGNIRMTDADGKITYANSLDLSDDYRDGFVDSLRVETEDQTRMAATRADR
ECCHHHEEECCCCEEEECCCCEEEEECCCCCCCCHHCCCCHHEEECCCCHHHHHHHHHHC
SKGNYTVFENGVYTACAPCKDDPKKPPLWQVKGARIIHDQTEKMLYFENAQLEFFGVPLA
CCCCEEEEECCEEEEECCCCCCCCCCCEEEECCCEEEECCCCEEEEEECCEEEEEECCHH
YIPYFSTPDPTVKRKSGFLMPGVTQTAGYGFGVDIPYYWAIAPDYDATFTPRITSKQGVL
HCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCEEEEECCCCCCEECCCCCCCCCEE
VQTEFRQRLENGGYQVRLYGIDQLNRNAFAGQPGDRQFRGGIDTKGQFALNDKWVFGWDG
EEHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCHHCCCCCCCCEEEECCCEEEEECC
ILLSDYTFLSDYRLAQYRDPFNSFMSLPTEAISQLFLTGVGSRSFFDVRAMHYLSFSGNQ
EEEECEEEHHCCHHHHHCCHHHHHHCCCHHHHHHHHHHCCCCCCEEEEEEEEEEEECCCC
QTVPVVYPVLDYSNVLNYPVFGGEFSYKTNFVNLTREQAVFDPITTTANTNGLCTTTSAD
EEEEEEEEECCCHHHHCCCEECCCCCEEECEEEECHHHHHCCCCCCCCCCCCEEEECCCC
PLARLPTQCLLRAMPGTYTRLTAQAEWRKSFTDPFGQIWTPFAILRADAINANVTAQPGV
HHHHCCHHHHHHCCCCCCEEEEECHHHHHHHCCHHHHHHCCHHHEEECCCCCCEEECCCC
GNFLPTGDTQALRLMPTVGLEYRYPFINVQPWGTTTIEPIAQVIIRPNETYAGRLPNEDA
CCCCCCCCCCEEEEEECCCCEEECCEEEEECCCCCHHHHHHHHEECCCCCEECCCCCCCH
QSMVFDASNLFSVDKFSGYDRIEGGGRANVGVQATTQFDRGGTINVLFGQSYHLFGLNSF
HHEEEECCCCEEEHHCCCCCCCCCCCCCCCCEEEEEEECCCCEEEEEECCEEEEEECCCE
AVQDMNNTGIGSGLDKTASDYVTRVDYSPNRTYTFSMRSRFDQQTLNVQRFEAEAKANFD
EEECCCCCCCCCCCCCCHHHHEEEEECCCCCEEEEHHHHCCCHHHCCHHEEEHHHHCCCC
RWSVALTYGNYAADPAIGYLNRREGILGSGSVKVAPNWVVTGAARWDLAANKINQYVIGA
EEEEEEEECCCCCCCHHHHHHCCCCCCCCCCEEECCCEEEECCCHHHHHHHCCCEEEEEC
GYVDDCFVLAANYVTSYNYTTGVTTPVLSHAYMLQIGLRTLANSSSTGAGGGIQ
CCHHHHHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA