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The map label for this gene is ksgA [H]

Identifier: 146340299

GI number: 146340299

Start: 3517696

End: 3518556

Strand: Reverse

Name: ksgA [H]

Synonym: BRADO3322

Alternate gene names: 146340299

Gene position: 3518556-3517696 (Counterclockwise)

Preceding gene: 146340300

Following gene: 146340298

Centisome position: 47.19

GC content: 67.83

Gene sequence:

>861_bases
ATGAGCGCGATCGACGACCTCCCGCCGCTGCGCGAGGTCATCCGCGAGCACCAGCTGTCGGCGCGCAAGGCGCTCGGCCA
GAACTTCCTGCTCGATCTCAACCTCACCGCCCGGATCGCGCGCGCCGCGGGTCCGCTCGAAGACGCCACCGTGGTCGAGA
TCGGCCCGGGACCCGGCGGCCTGACACGGGCCCTGCTGGCGCTCGGCGCCCGGCACGTCATCGCCGTCGAGCACGACGAG
CGCGCCATTCCCGCGCTGCGGACAATCGCCGATCGCTATCCCGGGCGGCTCGAGATCGTCTACACGGATGCCCGCACTTT
CGACGTCAGGCCCTATCTCGGCAGCACCAAAGCCAAGATCGTCGCCAACCTGCCCTACAACATCGCGACGCATCTGCTGA
TCGGCTGGCTGTCCGCCGAGCCGTGGCCGCCTTGGTACGAGATGATGGTGCTGATGTTCCAGCGCGAGGTCGCCGAGCGC
ATCGTGGCGACCGAGAACGAAGAGGCCTATGGGAGGCTCGGTGTGCTCGCCAATTGGCGTTGCGAGACCAAAATCCTGTT
CGACATCTCCCCATCGGCCTTCGTGCCGCCGCCGAAGGTCACATCGTCGGTGGTACGGCTGGTGCCGCGCCCTGCTCCGG
AGCCGTGCGACCGCCGTGCTCTCGAGCAGGTCGCCGCGGCGGCCTTCGGCCAGCGCCGCAAGATGCTGCGGCAAAGTCTC
AAATCGCTGCCAGCAGATCCCGCGCGGCTGGCGGCTGCGGCGGGCATCGATCCGACCCGCCGCGCCGAGACCATTCCGGT
GTCCGGCTTTGTTGCCATGGCGCGCGAATTGACCAATAGTCGCAACGACAACACGACCTAA

Upstream 100 bases:

>100_bases
ACCACGGCACGGCGTTCGGGATCGCCGGCAAGGGCAGCGCCAATCCGGCGAGTCTGATCGCGGCCCTGCAGCTCGCCGCG
CGCATGGCCCGCCAATCGTC

Downstream 100 bases:

>100_bases
CAAGAGCATTTCAGGGAGATTTCATCATGGCGTTGATGCGTCGCCAGTCGCTCGTGAAGTTCGATGCACCGCTGTGCGAG
ACCATCGTCGAGACACCGAA

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase [H]

Number of amino acids: Translated: 286; Mature: 285

Protein sequence:

>286_residues
MSAIDDLPPLREVIREHQLSARKALGQNFLLDLNLTARIARAAGPLEDATVVEIGPGPGGLTRALLALGARHVIAVEHDE
RAIPALRTIADRYPGRLEIVYTDARTFDVRPYLGSTKAKIVANLPYNIATHLLIGWLSAEPWPPWYEMMVLMFQREVAER
IVATENEEAYGRLGVLANWRCETKILFDISPSAFVPPPKVTSSVVRLVPRPAPEPCDRRALEQVAAAAFGQRRKMLRQSL
KSLPADPARLAAAAGIDPTRRAETIPVSGFVAMARELTNSRNDNTT

Sequences:

>Translated_286_residues
MSAIDDLPPLREVIREHQLSARKALGQNFLLDLNLTARIARAAGPLEDATVVEIGPGPGGLTRALLALGARHVIAVEHDE
RAIPALRTIADRYPGRLEIVYTDARTFDVRPYLGSTKAKIVANLPYNIATHLLIGWLSAEPWPPWYEMMVLMFQREVAER
IVATENEEAYGRLGVLANWRCETKILFDISPSAFVPPPKVTSSVVRLVPRPAPEPCDRRALEQVAAAAFGQRRKMLRQSL
KSLPADPARLAAAAGIDPTRRAETIPVSGFVAMARELTNSRNDNTT
>Mature_285_residues
SAIDDLPPLREVIREHQLSARKALGQNFLLDLNLTARIARAAGPLEDATVVEIGPGPGGLTRALLALGARHVIAVEHDER
AIPALRTIADRYPGRLEIVYTDARTFDVRPYLGSTKAKIVANLPYNIATHLLIGWLSAEPWPPWYEMMVLMFQREVAERI
VATENEEAYGRLGVLANWRCETKILFDISPSAFVPPPKVTSSVVRLVPRPAPEPCDRRALEQVAAAAFGQRRKMLRQSLK
SLPADPARLAAAAGIDPTRRAETIPVSGFVAMARELTNSRNDNTT

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits [H]

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily [H]

Homologues:

Organism=Homo sapiens, GI156415992, Length=292, Percent_Identity=37.6712328767123, Blast_Score=181, Evalue=9e-46,
Organism=Homo sapiens, GI7657198, Length=226, Percent_Identity=30.5309734513274, Blast_Score=97, Evalue=2e-20,
Organism=Escherichia coli, GI1786236, Length=274, Percent_Identity=39.051094890511, Blast_Score=149, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI25141369, Length=295, Percent_Identity=33.5593220338983, Blast_Score=150, Evalue=7e-37,
Organism=Caenorhabditis elegans, GI25146882, Length=213, Percent_Identity=31.924882629108, Blast_Score=94, Evalue=6e-20,
Organism=Saccharomyces cerevisiae, GI6324989, Length=239, Percent_Identity=31.3807531380753, Blast_Score=82, Evalue=1e-16,
Organism=Drosophila melanogaster, GI21357273, Length=249, Percent_Identity=34.9397590361446, Blast_Score=132, Evalue=2e-31,
Organism=Drosophila melanogaster, GI21358017, Length=231, Percent_Identity=32.034632034632, Blast_Score=97, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530 [H]

Pfam domain/function: PF00398 RrnaAD [H]

EC number: =2.1.1.182 [H]

Molecular weight: Translated: 31480; Mature: 31349

Theoretical pI: Translated: 9.42; Mature: 9.42

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAIDDLPPLREVIREHQLSARKALGQNFLLDLNLTARIARAAGPLEDATVVEIGPGPGG
CCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHCCCCCCCEEEEECCCCCH
LTRALLALGARHVIAVEHDERAIPALRTIADRYPGRLEIVYTDARTFDVRPYLGSTKAKI
HHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHCCCEEEEEEECCEEEECCCCCCCCCEEE
VANLPYNIATHLLIGWLSAEPWPPWYEMMVLMFQREVAERIVATENEEAYGRLGVLANWR
EEECCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCEEECCC
CETKILFDISPSAFVPPPKVTSSVVRLVPRPAPEPCDRRALEQVAAAAFGQRRKMLRQSL
CCEEEEEEECCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
KSLPADPARLAAAAGIDPTRRAETIPVSGFVAMARELTNSRNDNTT
HHCCCCHHHHHHHCCCCHHHCCCCCCHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SAIDDLPPLREVIREHQLSARKALGQNFLLDLNLTARIARAAGPLEDATVVEIGPGPGG
CCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHCCCCCCCEEEEECCCCCH
LTRALLALGARHVIAVEHDERAIPALRTIADRYPGRLEIVYTDARTFDVRPYLGSTKAKI
HHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHCCCEEEEEEECCEEEECCCCCCCCCEEE
VANLPYNIATHLLIGWLSAEPWPPWYEMMVLMFQREVAERIVATENEEAYGRLGVLANWR
EEECCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCEEECCC
CETKILFDISPSAFVPPPKVTSSVVRLVPRPAPEPCDRRALEQVAAAAFGQRRKMLRQSL
CCEEEEEEECCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
KSLPADPARLAAAAGIDPTRRAETIPVSGFVAMARELTNSRNDNTT
HHCCCCHHHHHHHCCCCHHHCCCCCCHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA