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The map label for this gene is arsB [H]

Identifier: 146340221

GI number: 146340221

Start: 3442304

End: 3443560

Strand: Reverse

Name: arsB [H]

Synonym: BRADO3240

Alternate gene names: 146340221

Gene position: 3443560-3442304 (Counterclockwise)

Preceding gene: 146340222

Following gene: 146340219

Centisome position: 46.18

GC content: 67.94

Gene sequence:

>1257_bases
ATGCTCGCACACCCGACCTGGCTCACCTACGCCATCATCATCACCGCCACCGCCGGCGTCATTATTCGCCCCTTCCGGCT
GCCCGAAGCAATCTGGGCGGTGGCCGGCGCGGTCCTGCTCGTCGCGCTCGGTCTGCTGCCTTGGACGGACGCGCTCGCCG
GCATCCGCAAGGGCATCGACGTCTATCTGTTCCTGATCGGCATGATGCTGATCGCCGAGCTCGCGCGCCGCGAAGGTCTG
TTCGATTATCTGGCCGCCTATGCGGTGGAGCATGCGCGCGGCTCGCCGCAGCTGCTGTTCCTGCTGGTCTATGCGGTCGG
CACCCTGGTCACCGTGCTGCTCTCCAACGACGCCACCGCGATCGTGCTGACGCCCGCCGTCTATGCCGCGACGCGCGCCG
CCGGCGCCTCGCCGCTGCCTTACCTCTACGTCTGCGCCTTCATCGCCAACGCGGCGAGCTTCGTGCTGCCAATCTCAAAC
CCGGCCAACCTCGTGGTGTTCGGCGAGCGCATGCCGCATCTGACCGAATGGCTGCGCCAGTTCACCCTGCCCTCGATTGC
GGCCATCGCCCTCACCTATGTGGCCTTGTGGCTGACCCAGCGCCGTGCGCTAAGCGACGAGACGCTGCGCACTGACGCCG
ACAAGCCCCATCTGAGCCGCGAGGGCCGGCTCGCGGCGATGGGCATCATCGCGATTGCCGCCGTGCTGCTGACGGCGTCC
GCGCTCGACGTGCAGCTGGGCCTGCCGACCTTCGTCTGCGGCGTGGTCACGGCGGCCGCGATCCTGCTGGTCAGCCGGCA
GTCGCCCTGGCCGATCCTCAAGGGCGTGTCCTGGGGCGTGCTGCCGCTGGTCGGCGGGCTGTTCGTGATGGTGGAGGGAT
TGGGCCGCCTTGGTGCGATCGGCCAGCTCAGCGCCTGGCTGCATCAGGGCGTCAGTCAGTCACCGGCGGGAACCGCCTGG
GCTGCCGGCCTGATCACGGCGGTCGCCGACAACATCGCCAACAATCTGCCGGTCGGGCTCGTCGCAGGCTCGGTCGCGGC
GAGCGATCACCTGCCGCAAGAAGTCATCCGCTCGATCCTGATCGGGGTCGATCTCGGGCCGAACATCTCGGTCACGGGCT
CGCTCGCGACCATCCTGTGGCTGACGGCGCTGCGGCGCGAAAAGATCGAGGTGTCGGCCTGGCAATTTCTCAAGATCGGA
CTCGTGGCAACGCCGCCAGCGCTGATTGCAGCACTAGCGGCGGCCATTCAAGGGTAG

Upstream 100 bases:

>100_bases
CTGCTGCTGCCCGAGACCGGCGGAGCGGAGGCCGCGCATCCGGACGCCGCCGCCGCGCCCGCTATCCCCGCCGAATGATT
TGCTCTAACGATCCCCGATC

Downstream 100 bases:

>100_bases
AGCGTGCGCGGTGATCAGGCAGCCGCCTCGTAGTTGACCTCGCTCTCGGCGACCTTGGTCAGGAGATCGTCGGTCTTCTT
CTCCTCGTTCAGGGTCTGGT

Product: putative arsenite permease

Products: ADP; phosphate; arsenite [Periplasm] [C]

Alternate protein names: Arsenic efflux pump protein [H]

Number of amino acids: Translated: 418; Mature: 418

Protein sequence:

>418_residues
MLAHPTWLTYAIIITATAGVIIRPFRLPEAIWAVAGAVLLVALGLLPWTDALAGIRKGIDVYLFLIGMMLIAELARREGL
FDYLAAYAVEHARGSPQLLFLLVYAVGTLVTVLLSNDATAIVLTPAVYAATRAAGASPLPYLYVCAFIANAASFVLPISN
PANLVVFGERMPHLTEWLRQFTLPSIAAIALTYVALWLTQRRALSDETLRTDADKPHLSREGRLAAMGIIAIAAVLLTAS
ALDVQLGLPTFVCGVVTAAAILLVSRQSPWPILKGVSWGVLPLVGGLFVMVEGLGRLGAIGQLSAWLHQGVSQSPAGTAW
AAGLITAVADNIANNLPVGLVAGSVAASDHLPQEVIRSILIGVDLGPNISVTGSLATILWLTALRREKIEVSAWQFLKIG
LVATPPALIAALAAAIQG

Sequences:

>Translated_418_residues
MLAHPTWLTYAIIITATAGVIIRPFRLPEAIWAVAGAVLLVALGLLPWTDALAGIRKGIDVYLFLIGMMLIAELARREGL
FDYLAAYAVEHARGSPQLLFLLVYAVGTLVTVLLSNDATAIVLTPAVYAATRAAGASPLPYLYVCAFIANAASFVLPISN
PANLVVFGERMPHLTEWLRQFTLPSIAAIALTYVALWLTQRRALSDETLRTDADKPHLSREGRLAAMGIIAIAAVLLTAS
ALDVQLGLPTFVCGVVTAAAILLVSRQSPWPILKGVSWGVLPLVGGLFVMVEGLGRLGAIGQLSAWLHQGVSQSPAGTAW
AAGLITAVADNIANNLPVGLVAGSVAASDHLPQEVIRSILIGVDLGPNISVTGSLATILWLTALRREKIEVSAWQFLKIG
LVATPPALIAALAAAIQG
>Mature_418_residues
MLAHPTWLTYAIIITATAGVIIRPFRLPEAIWAVAGAVLLVALGLLPWTDALAGIRKGIDVYLFLIGMMLIAELARREGL
FDYLAAYAVEHARGSPQLLFLLVYAVGTLVTVLLSNDATAIVLTPAVYAATRAAGASPLPYLYVCAFIANAASFVLPISN
PANLVVFGERMPHLTEWLRQFTLPSIAAIALTYVALWLTQRRALSDETLRTDADKPHLSREGRLAAMGIIAIAAVLLTAS
ALDVQLGLPTFVCGVVTAAAILLVSRQSPWPILKGVSWGVLPLVGGLFVMVEGLGRLGAIGQLSAWLHQGVSQSPAGTAW
AAGLITAVADNIANNLPVGLVAGSVAASDHLPQEVIRSILIGVDLGPNISVTGSLATILWLTALRREKIEVSAWQFLKIG
LVATPPALIAALAAAIQG

Specific function: Involved in arsenical resistance. Thought to form the channel of an arsenite pump [H]

COG id: COG1055

COG function: function code P; Na+/H+ antiporter NhaD and related arsenite permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ArsB family [H]

Homologues:

Organism=Escherichia coli, GI87082277, Length=369, Percent_Identity=28.4552845528455, Blast_Score=107, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000802 [H]

Pfam domain/function: PF02040 ArsB [H]

EC number: NA

Molecular weight: Translated: 43950; Mature: 43950

Theoretical pI: Translated: 8.22; Mature: 8.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAHPTWLTYAIIITATAGVIIRPFRLPEAIWAVAGAVLLVALGLLPWTDALAGIRKGID
CCCCCHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
VYLFLIGMMLIAELARREGLFDYLAAYAVEHARGSPQLLFLLVYAVGTLVTVLLSNDATA
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCE
IVLTPAVYAATRAAGASPLPYLYVCAFIANAASFVLPISNPANLVVFGERMPHLTEWLRQ
EEECHHHHHHHHHCCCCCCHHHHHHHHHHHHHHEEEECCCCCCEEEECCCCCHHHHHHHH
FTLPSIAAIALTYVALWLTQRRALSDETLRTDADKPHLSREGRLAAMGIIAIAAVLLTAS
HCCHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
ALDVQLGLPTFVCGVVTAAAILLVSRQSPWPILKGVSWGVLPLVGGLFVMVEGLGRLGAI
HHHEECCCHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHCCCH
GQLSAWLHQGVSQSPAGTAWAAGLITAVADNIANNLPVGLVAGSVAASDHLPQEVIRSIL
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHCCCHHHCCCCHHHHHHHHH
IGVDLGPNISVTGSLATILWLTALRREKIEVSAWQFLKIGLVATPPALIAALAAAIQG
HCCCCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MLAHPTWLTYAIIITATAGVIIRPFRLPEAIWAVAGAVLLVALGLLPWTDALAGIRKGID
CCCCCHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
VYLFLIGMMLIAELARREGLFDYLAAYAVEHARGSPQLLFLLVYAVGTLVTVLLSNDATA
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCE
IVLTPAVYAATRAAGASPLPYLYVCAFIANAASFVLPISNPANLVVFGERMPHLTEWLRQ
EEECHHHHHHHHHCCCCCCHHHHHHHHHHHHHHEEEECCCCCCEEEECCCCCHHHHHHHH
FTLPSIAAIALTYVALWLTQRRALSDETLRTDADKPHLSREGRLAAMGIIAIAAVLLTAS
HCCHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
ALDVQLGLPTFVCGVVTAAAILLVSRQSPWPILKGVSWGVLPLVGGLFVMVEGLGRLGAI
HHHEECCCHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHCCCH
GQLSAWLHQGVSQSPAGTAWAAGLITAVADNIANNLPVGLVAGSVAASDHLPQEVIRSIL
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHCCCHHHCCCCHHHHHHHHH
IGVDLGPNISVTGSLATILWLTALRREKIEVSAWQFLKIGLVATPPALIAALAAAIQG
HCCCCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; arsenite [Cytoplasm]; H2O [C]

Specific reaction: ATP + arsenite [Cytoplasm] + H2O = ADP + phosphate + arsenite [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12950922 [H]