Click here to switch to the map view.

The map label for this gene is guaA

Identifier: 146340154

GI number: 146340154

Start: 3373161

End: 3374768

Strand: Direct

Name: guaA

Synonym: BRADO3169

Alternate gene names: 146340154

Gene position: 3373161-3374768 (Clockwise)

Preceding gene: 146340153

Following gene: 146340155

Centisome position: 45.24

GC content: 66.54

Gene sequence:

>1608_bases
ATGACAGCCCCAGCCAACAATACCCCCGCCGCCGCCGCCACCGACACCTCCGTGGCCGCGCTCCACGACAAGATCCTGAT
CGTCGATTTCGGCAGCCAGGTCACGCAGCTGATCGCGCGCCGGGTGCGCGAGGACGGCGTGTATTGCGAGATCGTGCCGT
TCCAGAAGGCGGAGGCCGCCTTCCGGCAGATGAACCCGAAGGCCGTGATCCTGTCCGGCGGCCCCGAATCCGTCCACGAG
GAAGGCTCGCCGCGCGCCCCGCAGCTGATCTTCGAGTCAGGCGTCCCCGTGATGGGCATCTGCTACGGCCAGATGACGAT
GGCCGCCCAGCTCGGCGGCACCGTCGAGGGCGGTCACCACCGCGAGTTCGGCCGCGCCGACGTCGAGGTGAAGACCAACA
GCCTGCTGTTCGACGGCGTCTGGCAGCCGGGTGAGCAGCACCAGGTGTGGATGAGCCATGGCGACCGCATCACCCAGATG
CCGCCGGGCTTCTCGGTCGCCGGCGTCTCGCCGAACGCGCCGTTCGCCGTGATCCAGGACGAGGCGCGCAAATATTACGG
CCTGATGTTCCACCCCGAGGTGGTGCACACACCCGACGGCGCCAAGCTGATCCGCAACTTCGTCCGCAACATCTCAGGCC
TCGGCGGCGACTGGACCATGCACGCCTTCCGCGAGGAGGAGATCAAGAAGATCCGCGCCCAGGTCGGCCAGGGCAAGGTG
ATCTGCGGTCTCTCCGGCGGCGTCGATTCCGCTGTCGCCGCGGTGCTGATCCACGAGGCGATCGGCGACCAGCTCACCTG
CGTCTTCGTCGACCACGGCCTGCTGCGCCTGAACGAGGCCGAGACCGTCGTCGACCTGTTCCGCCACCACTACAACATCC
CGCTGGTGCATGTGGACGCCTCAAAGCTCTTCCTCGGCGAGCTCGCAGGCGTCACCGACCCGGAAGCGAAGCGCAAGACC
ATCGGAAGGCTCTTCATCGACGTGTTCGAGGCCGAGGCCAAGAAGATCGGCGGCGCCGATTTCCTGGCGCAGGGCACGCT
CTATCCCGACGTGATCGAGAGCGTCTCCTTCACCGGCGGCCCCTCGGTGACGATCAAGTCGCACCACAATGTCGGCGGCC
TGCCGGCGCGCATGAACATGAAGCTGGTCGAGCCGCTGCGCGAGCTGTTCAAGGACGAGGTCCGCGTGCTCGGCCGCGAG
CTCGGCCTCCCCGACGTCTTCGTCGGCCGCCACCCGTTCCCGGGCCCCGGCCTCGCCATCCGCTGCCCCGGCGAGATCAC
GACAGACAAGCTCGACATCCTGCGCAAGGCCGACGCCATCTACATCGACGAGATCCGCAAAGCCGGCCTCTACGACACCA
TCTGGCAGGCCTTCGCCGTGCTGCTCCCCGTCAAGACCGTCGGCGTCATGGGCGACGGCCGCACCTACGACTACGTCGTC
GGCCTGCGCGCGGTCACCTCGACGGATGGCATGACGGCGGACTTCTATCCGTTTGATATGAAGTTCTTAGGGAATACGGC
GACGCGCATCATCAACGAGGTGAAGGGCGTGAACCGGGTGGTGTATGACGTGACGAGCAAGCCTCCGGGAACGATTGAGT
GGGAGTAG

Upstream 100 bases:

>100_bases
GGGCGCTGTATCTGGCAGCCGCATCGAAACGCGTCACGGCGCAGTGAATCGCCCGCTTGCAAGGGTTGCGAGTCCTCCCG
CAGCGGCGTAATTCCTTGCC

Downstream 100 bases:

>100_bases
GGTGCCCCAATGACGCTCGAATTGACGTGTCCCCCAACAAGGCTGTGCACCGCAAGATTACCGTGATCTTAACAGCCCAC
TTGACTTTTTTCGGTAGTAG

Product: GMP synthase

Products: NA

Alternate protein names: GMP synthetase; Glutamine amidotransferase

Number of amino acids: Translated: 535; Mature: 534

Protein sequence:

>535_residues
MTAPANNTPAAAATDTSVAALHDKILIVDFGSQVTQLIARRVREDGVYCEIVPFQKAEAAFRQMNPKAVILSGGPESVHE
EGSPRAPQLIFESGVPVMGICYGQMTMAAQLGGTVEGGHHREFGRADVEVKTNSLLFDGVWQPGEQHQVWMSHGDRITQM
PPGFSVAGVSPNAPFAVIQDEARKYYGLMFHPEVVHTPDGAKLIRNFVRNISGLGGDWTMHAFREEEIKKIRAQVGQGKV
ICGLSGGVDSAVAAVLIHEAIGDQLTCVFVDHGLLRLNEAETVVDLFRHHYNIPLVHVDASKLFLGELAGVTDPEAKRKT
IGRLFIDVFEAEAKKIGGADFLAQGTLYPDVIESVSFTGGPSVTIKSHHNVGGLPARMNMKLVEPLRELFKDEVRVLGRE
LGLPDVFVGRHPFPGPGLAIRCPGEITTDKLDILRKADAIYIDEIRKAGLYDTIWQAFAVLLPVKTVGVMGDGRTYDYVV
GLRAVTSTDGMTADFYPFDMKFLGNTATRIINEVKGVNRVVYDVTSKPPGTIEWE

Sequences:

>Translated_535_residues
MTAPANNTPAAAATDTSVAALHDKILIVDFGSQVTQLIARRVREDGVYCEIVPFQKAEAAFRQMNPKAVILSGGPESVHE
EGSPRAPQLIFESGVPVMGICYGQMTMAAQLGGTVEGGHHREFGRADVEVKTNSLLFDGVWQPGEQHQVWMSHGDRITQM
PPGFSVAGVSPNAPFAVIQDEARKYYGLMFHPEVVHTPDGAKLIRNFVRNISGLGGDWTMHAFREEEIKKIRAQVGQGKV
ICGLSGGVDSAVAAVLIHEAIGDQLTCVFVDHGLLRLNEAETVVDLFRHHYNIPLVHVDASKLFLGELAGVTDPEAKRKT
IGRLFIDVFEAEAKKIGGADFLAQGTLYPDVIESVSFTGGPSVTIKSHHNVGGLPARMNMKLVEPLRELFKDEVRVLGRE
LGLPDVFVGRHPFPGPGLAIRCPGEITTDKLDILRKADAIYIDEIRKAGLYDTIWQAFAVLLPVKTVGVMGDGRTYDYVV
GLRAVTSTDGMTADFYPFDMKFLGNTATRIINEVKGVNRVVYDVTSKPPGTIEWE
>Mature_534_residues
TAPANNTPAAAATDTSVAALHDKILIVDFGSQVTQLIARRVREDGVYCEIVPFQKAEAAFRQMNPKAVILSGGPESVHEE
GSPRAPQLIFESGVPVMGICYGQMTMAAQLGGTVEGGHHREFGRADVEVKTNSLLFDGVWQPGEQHQVWMSHGDRITQMP
PGFSVAGVSPNAPFAVIQDEARKYYGLMFHPEVVHTPDGAKLIRNFVRNISGLGGDWTMHAFREEEIKKIRAQVGQGKVI
CGLSGGVDSAVAAVLIHEAIGDQLTCVFVDHGLLRLNEAETVVDLFRHHYNIPLVHVDASKLFLGELAGVTDPEAKRKTI
GRLFIDVFEAEAKKIGGADFLAQGTLYPDVIESVSFTGGPSVTIKSHHNVGGLPARMNMKLVEPLRELFKDEVRVLGREL
GLPDVFVGRHPFPGPGLAIRCPGEITTDKLDILRKADAIYIDEIRKAGLYDTIWQAFAVLLPVKTVGVMGDGRTYDYVVG
LRAVTSTDGMTADFYPFDMKFLGNTATRIINEVKGVNRVVYDVTSKPPGTIEWE

Specific function: Catalyzes the synthesis of GMP from XMP

COG id: COG0519

COG function: function code F; GMP synthase, PP-ATPase domain/subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GMP-binding domain

Homologues:

Organism=Homo sapiens, GI4504035, Length=549, Percent_Identity=36.4298724954463, Blast_Score=292, Evalue=7e-79,
Organism=Escherichia coli, GI1788854, Length=517, Percent_Identity=56.6731141199226, Blast_Score=600, Evalue=1e-173,
Organism=Caenorhabditis elegans, GI71992710, Length=570, Percent_Identity=36.6666666666667, Blast_Score=292, Evalue=3e-79,
Organism=Caenorhabditis elegans, GI71992717, Length=570, Percent_Identity=36.6666666666667, Blast_Score=292, Evalue=4e-79,
Organism=Caenorhabditis elegans, GI133901714, Length=570, Percent_Identity=36.6666666666667, Blast_Score=291, Evalue=7e-79,
Organism=Saccharomyces cerevisiae, GI6323873, Length=530, Percent_Identity=52.6415094339623, Blast_Score=532, Evalue=1e-152,
Organism=Drosophila melanogaster, GI281365319, Length=550, Percent_Identity=35.8181818181818, Blast_Score=283, Evalue=3e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GUAA_BRASO (A4YSU1)

Other databases:

- EMBL:   CU234118
- RefSeq:   YP_001205202.1
- ProteinModelPortal:   A4YSU1
- STRING:   A4YSU1
- GeneID:   5117070
- GenomeReviews:   CU234118_GR
- KEGG:   bra:BRADO3169
- eggNOG:   COG0519
- HOGENOM:   HBG391775
- OMA:   MKLVEPL
- ProtClustDB:   PRK00074
- BioCyc:   BSP376:BRADO3169-MONOMER
- HAMAP:   MF_00344
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR001674
- InterPro:   IPR004739
- InterPro:   IPR022955
- InterPro:   IPR022310
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PRINTS:   PR00096
- TIGRFAMs:   TIGR00884
- TIGRFAMs:   TIGR00888

Pfam domain/function: PF00117 GATase; PF00958 GMP_synt_C; PF02540 NAD_synthase

EC number: =6.3.5.2

Molecular weight: Translated: 58292; Mature: 58160

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: PS51273 GATASE_TYPE_1

Important sites: ACT_SITE 101-101 ACT_SITE 191-191 ACT_SITE 193-193

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAPANNTPAAAATDTSVAALHDKILIVDFGSQVTQLIARRVREDGVYCEIVPFQKAEAA
CCCCCCCCCCCCCCCCHHHHHCCEEEEEECCHHHHHHHHHHHHHCCCEEEEECCHHHHHH
FRQMNPKAVILSGGPESVHEEGSPRAPQLIFESGVPVMGICYGQMTMAAQLGGTVEGGHH
HHHCCCCEEEECCCCHHHHCCCCCCCCHHHHHCCCCEEEEEHHHHHHHHHCCCCCCCCCC
REFGRADVEVKTNSLLFDGVWQPGEQHQVWMSHGDRITQMPPGFSVAGVSPNAPFAVIQD
CCCCCCEEEEEECEEEEECCCCCCCCCCHHHHCCCEEECCCCCCEEECCCCCCCEEEEEH
EARKYYGLMFHPEVVHTPDGAKLIRNFVRNISGLGGDWTMHAFREEEIKKIRAQVGQGKV
HHHHHHCEEECCCEEECCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCE
ICGLSGGVDSAVAAVLIHEAIGDQLTCVFVDHGLLRLNEAETVVDLFRHHYNIPLVHVDA
EEEECCCHHHHHHHHHHHHHHCCCEEEEEECCCEEEECHHHHHHHHHHHHCCCCEEEECH
SKLFLGELAGVTDPEAKRKTIGRLFIDVFEAEAKKIGGADFLAQGTLYPDVIESVSFTGG
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHEECCCCCHHHHHHCCCCCC
PSVTIKSHHNVGGLPARMNMKLVEPLRELFKDEVRVLGRELGLPDVFVGRHPFPGPGLAI
CEEEEECCCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCEEE
RCPGEITTDKLDILRKADAIYIDEIRKAGLYDTIWQAFAVLLPVKTVGVMGDGRTYDYVV
ECCCCCCHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHEEEECCCCCEEEEE
GLRAVTSTDGMTADFYPFDMKFLGNTATRIINEVKGVNRVVYDVTSKPPGTIEWE
EEEEEECCCCCEECCCCCCHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCC
>Mature Secondary Structure 
TAPANNTPAAAATDTSVAALHDKILIVDFGSQVTQLIARRVREDGVYCEIVPFQKAEAA
CCCCCCCCCCCCCCCHHHHHCCEEEEEECCHHHHHHHHHHHHHCCCEEEEECCHHHHHH
FRQMNPKAVILSGGPESVHEEGSPRAPQLIFESGVPVMGICYGQMTMAAQLGGTVEGGHH
HHHCCCCEEEECCCCHHHHCCCCCCCCHHHHHCCCCEEEEEHHHHHHHHHCCCCCCCCCC
REFGRADVEVKTNSLLFDGVWQPGEQHQVWMSHGDRITQMPPGFSVAGVSPNAPFAVIQD
CCCCCCEEEEEECEEEEECCCCCCCCCCHHHHCCCEEECCCCCCEEECCCCCCCEEEEEH
EARKYYGLMFHPEVVHTPDGAKLIRNFVRNISGLGGDWTMHAFREEEIKKIRAQVGQGKV
HHHHHHCEEECCCEEECCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCE
ICGLSGGVDSAVAAVLIHEAIGDQLTCVFVDHGLLRLNEAETVVDLFRHHYNIPLVHVDA
EEEECCCHHHHHHHHHHHHHHCCCEEEEEECCCEEEECHHHHHHHHHHHHCCCCEEEECH
SKLFLGELAGVTDPEAKRKTIGRLFIDVFEAEAKKIGGADFLAQGTLYPDVIESVSFTGG
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHEECCCCCHHHHHHCCCCCC
PSVTIKSHHNVGGLPARMNMKLVEPLRELFKDEVRVLGRELGLPDVFVGRHPFPGPGLAI
CEEEEECCCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCEEE
RCPGEITTDKLDILRKADAIYIDEIRKAGLYDTIWQAFAVLLPVKTVGVMGDGRTYDYVV
ECCCCCCHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHEEEECCCCCEEEEE
GLRAVTSTDGMTADFYPFDMKFLGNTATRIINEVKGVNRVVYDVTSKPPGTIEWE
EEEEEECCCCCEECCCCCCHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA