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The map label for this gene is alkB [H]

Identifier: 146339598

GI number: 146339598

Start: 2774348

End: 2775007

Strand: Direct

Name: alkB [H]

Synonym: BRADO2590

Alternate gene names: 146339598

Gene position: 2774348-2775007 (Clockwise)

Preceding gene: 146339597

Following gene: 146339604

Centisome position: 37.21

GC content: 69.85

Gene sequence:

>660_bases
GTGAGGCGGATGACGGCGGATTTGTTTGATGGTCTTGATGCCGGGCCTGCGCGCGAGCTGCTTGCGCCGGGCGCGCTGCT
GCTGCGCGGCTTCGCGCGGCCACTTGAGACCGAGCTGCTCGCGGCGATCGAGATGATCACGGCGCAGGCGCCGTTCCGCC
GCATGGTCACGCCGGGCGGCCACCAGATGTCGGTGGCGATGACCAATTGCGGCGCGCTCGGCTGGGTGACGGATCGCAGC
GGCTATCGTTACGATGCCGTCGATCCGGAGAGCGGACAGCCCTGGCCGGCGATGCCGCCGCTGTTCCGTCAGATCGCCGA
GAATGCCGCGCGCGAGGCTGGCTTTCCAGGTTTCGCGCCCGACGCCTGCCTGATCAACCGCTACGAGCCCGGCGCAAAAA
TGTCGCTGCATCAGGACCGCGACGAGCGCGACGTCGGCGCGCCGATCGTCTCGGTGTCGCTGGGGCTGCCCGCGACGTTC
CTGTTCGGCGGCCTGAGGCGCACCGACAAGACGCAGCGTTATCGGTTGGTTCATGGCGACGTCGTCGTCTGGGGCGGGCC
GGCGCGGCTCGCGTTTCACGGCGTCGCGCCGCTCGCCGACGGCGAGCATGCGCGGCTTGGCCGCCGGCGTATCAACCTGA
CCTTTCGCCGCGCGGGTTGA

Upstream 100 bases:

>100_bases
AGGGCACGCGCGGCAGCTACCGCGTCACCATGCGCCACGGCGTCAGCCGGCTGCGCGCCGGCCGGCGGCATACGGTCGGC
GTGATCTTTCACGATGCGAA

Downstream 100 bases:

>100_bases
TCAGGCCGGCTTGGCCGGATGAATGAAGGCCCAGGGCGAGACTTCGCTGTCGCGCGGCACCTCGATGTCGATCAGATACG
GCCCGCCATGCGCGAGTGCG

Product: alkylated DNA repair protein AlkB

Products: NA

Alternate protein names: Alkylated DNA repair protein AlkB [H]

Number of amino acids: Translated: 219; Mature: 219

Protein sequence:

>219_residues
MRRMTADLFDGLDAGPARELLAPGALLLRGFARPLETELLAAIEMITAQAPFRRMVTPGGHQMSVAMTNCGALGWVTDRS
GYRYDAVDPESGQPWPAMPPLFRQIAENAAREAGFPGFAPDACLINRYEPGAKMSLHQDRDERDVGAPIVSVSLGLPATF
LFGGLRRTDKTQRYRLVHGDVVVWGGPARLAFHGVAPLADGEHARLGRRRINLTFRRAG

Sequences:

>Translated_219_residues
MRRMTADLFDGLDAGPARELLAPGALLLRGFARPLETELLAAIEMITAQAPFRRMVTPGGHQMSVAMTNCGALGWVTDRS
GYRYDAVDPESGQPWPAMPPLFRQIAENAAREAGFPGFAPDACLINRYEPGAKMSLHQDRDERDVGAPIVSVSLGLPATF
LFGGLRRTDKTQRYRLVHGDVVVWGGPARLAFHGVAPLADGEHARLGRRRINLTFRRAG
>Mature_219_residues
MRRMTADLFDGLDAGPARELLAPGALLLRGFARPLETELLAAIEMITAQAPFRRMVTPGGHQMSVAMTNCGALGWVTDRS
GYRYDAVDPESGQPWPAMPPLFRQIAENAAREAGFPGFAPDACLINRYEPGAKMSLHQDRDERDVGAPIVSVSLGLPATF
LFGGLRRTDKTQRYRLVHGDVVVWGGPARLAFHGVAPLADGEHARLGRRRINLTFRRAG

Specific function: Dioxygenase that repairs alkylated DNA and RNA containing 3-methylcytosine or 1-methyladenine by oxidative demethylation. Has highest activity towards 3-methylcytosine. Has lower activity towards alkylated DNA containing ethenoadenine, and no detectable a

COG id: COG3145

COG function: function code L; Alkylated DNA repair protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Fe2OG dioxygenase domain [H]

Homologues:

Organism=Homo sapiens, GI87298840, Length=123, Percent_Identity=31.7073170731707, Blast_Score=66, Evalue=2e-11,
Organism=Escherichia coli, GI1788541, Length=213, Percent_Identity=59.1549295774648, Blast_Score=254, Evalue=3e-69,
Organism=Drosophila melanogaster, GI45555401, Length=131, Percent_Identity=32.0610687022901, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004574 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 23903; Mature: 23903

Theoretical pI: Translated: 9.65; Mature: 9.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRMTADLFDGLDAGPARELLAPGALLLRGFARPLETELLAAIEMITAQAPFRRMVTPGG
CCCCCHHHHCCCCCCCHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHCCCCC
HQMSVAMTNCGALGWVTDRSGYRYDAVDPESGQPWPAMPPLFRQIAENAAREAGFPGFAP
CEEEEEEECCCEEEEEECCCCCEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCC
DACLINRYEPGAKMSLHQDRDERDVGAPIVSVSLGLPATFLFGGLRRTDKTQRYRLVHGD
CHHEEECCCCCCCCCCCCCCCCHHHCCCEEEEECCCCHHHHHCCHHCCCCCCEEEEEECC
VVVWGGPARLAFHGVAPLADGEHARLGRRRINLTFRRAG
EEEECCCHHEEEECCCCCCCCCHHHHCCEEEEEEEEECC
>Mature Secondary Structure
MRRMTADLFDGLDAGPARELLAPGALLLRGFARPLETELLAAIEMITAQAPFRRMVTPGG
CCCCCHHHHCCCCCCCHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHCCCCC
HQMSVAMTNCGALGWVTDRSGYRYDAVDPESGQPWPAMPPLFRQIAENAAREAGFPGFAP
CEEEEEEECCCEEEEEECCCCCEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCC
DACLINRYEPGAKMSLHQDRDERDVGAPIVSVSLGLPATFLFGGLRRTDKTQRYRLVHGD
CHHEEECCCCCCCCCCCCCCCCHHHCCCEEEEECCCCHHHHHCCHHCCCCCCEEEEEECC
VVVWGGPARLAFHGVAPLADGEHARLGRRRINLTFRRAG
EEEECCCHHEEEECCCCCCCCCHHHHCCEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3536913; 9097040; 9278503; 3887409; 7928996 [H]