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The map label for this gene is lcfA [H]

Identifier: 146339588

GI number: 146339588

Start: 2761204

End: 2762613

Strand: Direct

Name: lcfA [H]

Synonym: BRADO2579

Alternate gene names: 146339588

Gene position: 2761204-2762613 (Clockwise)

Preceding gene: 146339587

Following gene: 146339589

Centisome position: 37.03

GC content: 65.11

Gene sequence:

>1410_bases
ATGCTGATGCGCAACGACATCGCCTTCATCGAGGCCGCCTATGGCGCGATGCGGCTCGGCGCCTATGGCGTGCCGGTGAA
CTGGCACTTCAAGCCGGAGGAGATCGACTACATCCTGAAGGATTCGGCGACGCGGGTGCTGATCGGTCATGCCGATCTCT
TGCACCAGCTTCGCGGTCTTATTCCACCTGGCGTCACCGTCCTCAGCGTGCCGACACCGCCGGAGATCCTGTCCAACTAC
AAGATCTCTGCAGATTTGCTTCAGCCGCCTGAATTTGCCATCGAGCTGGAGAGCTGGCTCGTTCAGCACAGCCCCTACGA
AGGGCCGCAGGTGCCGCAGCCGCAGAACATGATCTACACCTCCGGCACGACCGGCCATCCCAAGGGCGTCCGCCGCAGCG
CGCCGACGGTGGAGCAGGCGGCGTCGGCCGAGCGGATGCGGGCGCTGATCTACGGGCTGAAGCCGGGCTCGCGCGCGCTG
CTGCCAGGCCCGCTGTATCATTCGGCGCCGAACGCCTTCGGCCTGCGTGCCGGCCGGCTCGGCGGCGCGCTGGTGCTGAT
GCCGCGCTTCGAGCCGGAAGAGTTTCTCTCCGTCATCCAGTCCGAGCGGATCGACACCATCTTCATGGTGCCGACCATGT
TCATCCGGCTGATGAAGCTGCCGGAGGAGGTGCGCCGCCGCTTTGTCGTCTCCTCGCTGCGTCACGTGATTCACGCCGCC
GCTCCGTGCCCGGCCGACGTCAAGCGCGCGATGATCGAGTGGTGGGGCCCCGTGATCTACGAATTCTACGGCTCGACCGA
ATCGGGCGCGGTGACCTTCGCGACCTCCGAGGACGCGCTGCGAAAGCCCGGCACCGTCGGGAAGGTCTCGCCCGGCGCCG
AGCTGCGCTTCCTCGGCGACGACGGCCGCATCCTGCCGCAGGGTGAGATCGGCGAGATCTATTCGCGTATCGCGGGCAAT
CCGGACTTCACCTATCACAACAAGCCTGAGAAGCGCGCCGAGATCGATCGCGACGGCTTCATCACGTCGGGTGATGTCGG
CTACATGGACGCCGAGGGATATGTCTTCCTGTGCGACCGCAAGCGCGACATGGTGATCTCCGGCGGCGTCAACATCTATC
CGGCCGAGATCGAGGCCGTGCTGCACGCGGTGCCCGGCGTGCATGATTGCGCCGTTTTCGGCGTTCCCGACGCCGAGTTC
GGCGAGGCGCTGATGGCGGTGGTCGAGCCGCAACCGGGCGTGGTGCTCGATACTGGCGAGATCAAGCTGCGGCTGAAGGC
CTCTTTGGCCGACTACAAGGTGCCCAGGCAAATTGAAATCCAGGCGAAATTGCCACGCGAGGATTCCGGCAAGATATTCA
AGCGGCGGCTGCGCGATCCCTATTGGGAGCAGGCGGGGCGGAAGATCTGA

Upstream 100 bases:

>100_bases
CGGCGACCGCCGGCGCAGCCATCCTGAGATCGCCGACCGCGCCGCGCGGATCGCCAGTGGTCTCGCGCAACTCGGCGTGC
GCCAAGGCGATTGCGTCTGC

Downstream 100 bases:

>100_bases
CCGTGTGCGTGCTGGTCGAGCGCATCGAATAAGGCAGGCGACGCCTCTTTCTTGATCTTGCGCTGCAATGCAGATACGGC
AGACTGACCCGTAAATGGGC

Product: long-chain-fatty-acid--CoA ligase

Products: NA

Alternate protein names: Long-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 469; Mature: 469

Protein sequence:

>469_residues
MLMRNDIAFIEAAYGAMRLGAYGVPVNWHFKPEEIDYILKDSATRVLIGHADLLHQLRGLIPPGVTVLSVPTPPEILSNY
KISADLLQPPEFAIELESWLVQHSPYEGPQVPQPQNMIYTSGTTGHPKGVRRSAPTVEQAASAERMRALIYGLKPGSRAL
LPGPLYHSAPNAFGLRAGRLGGALVLMPRFEPEEFLSVIQSERIDTIFMVPTMFIRLMKLPEEVRRRFVVSSLRHVIHAA
APCPADVKRAMIEWWGPVIYEFYGSTESGAVTFATSEDALRKPGTVGKVSPGAELRFLGDDGRILPQGEIGEIYSRIAGN
PDFTYHNKPEKRAEIDRDGFITSGDVGYMDAEGYVFLCDRKRDMVISGGVNIYPAEIEAVLHAVPGVHDCAVFGVPDAEF
GEALMAVVEPQPGVVLDTGEIKLRLKASLADYKVPRQIEIQAKLPREDSGKIFKRRLRDPYWEQAGRKI

Sequences:

>Translated_469_residues
MLMRNDIAFIEAAYGAMRLGAYGVPVNWHFKPEEIDYILKDSATRVLIGHADLLHQLRGLIPPGVTVLSVPTPPEILSNY
KISADLLQPPEFAIELESWLVQHSPYEGPQVPQPQNMIYTSGTTGHPKGVRRSAPTVEQAASAERMRALIYGLKPGSRAL
LPGPLYHSAPNAFGLRAGRLGGALVLMPRFEPEEFLSVIQSERIDTIFMVPTMFIRLMKLPEEVRRRFVVSSLRHVIHAA
APCPADVKRAMIEWWGPVIYEFYGSTESGAVTFATSEDALRKPGTVGKVSPGAELRFLGDDGRILPQGEIGEIYSRIAGN
PDFTYHNKPEKRAEIDRDGFITSGDVGYMDAEGYVFLCDRKRDMVISGGVNIYPAEIEAVLHAVPGVHDCAVFGVPDAEF
GEALMAVVEPQPGVVLDTGEIKLRLKASLADYKVPRQIEIQAKLPREDSGKIFKRRLRDPYWEQAGRKI
>Mature_469_residues
MLMRNDIAFIEAAYGAMRLGAYGVPVNWHFKPEEIDYILKDSATRVLIGHADLLHQLRGLIPPGVTVLSVPTPPEILSNY
KISADLLQPPEFAIELESWLVQHSPYEGPQVPQPQNMIYTSGTTGHPKGVRRSAPTVEQAASAERMRALIYGLKPGSRAL
LPGPLYHSAPNAFGLRAGRLGGALVLMPRFEPEEFLSVIQSERIDTIFMVPTMFIRLMKLPEEVRRRFVVSSLRHVIHAA
APCPADVKRAMIEWWGPVIYEFYGSTESGAVTFATSEDALRKPGTVGKVSPGAELRFLGDDGRILPQGEIGEIYSRIAGN
PDFTYHNKPEKRAEIDRDGFITSGDVGYMDAEGYVFLCDRKRDMVISGGVNIYPAEIEAVLHAVPGVHDCAVFGVPDAEF
GEALMAVVEPQPGVVLDTGEIKLRLKASLADYKVPRQIEIQAKLPREDSGKIFKRRLRDPYWEQAGRKI

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=491, Percent_Identity=27.9022403258656, Blast_Score=163, Evalue=4e-40,
Organism=Homo sapiens, GI187761345, Length=498, Percent_Identity=26.7068273092369, Blast_Score=142, Evalue=9e-34,
Organism=Homo sapiens, GI187761343, Length=498, Percent_Identity=26.7068273092369, Blast_Score=142, Evalue=9e-34,
Organism=Homo sapiens, GI42544132, Length=391, Percent_Identity=29.156010230179, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI38505220, Length=468, Percent_Identity=27.7777777777778, Blast_Score=139, Evalue=5e-33,
Organism=Homo sapiens, GI122937307, Length=473, Percent_Identity=28.1183932346723, Blast_Score=129, Evalue=4e-30,
Organism=Homo sapiens, GI115511026, Length=467, Percent_Identity=26.5524625267666, Blast_Score=122, Evalue=1e-27,
Organism=Homo sapiens, GI157311624, Length=477, Percent_Identity=26.2054507337526, Blast_Score=117, Evalue=3e-26,
Organism=Homo sapiens, GI157311622, Length=477, Percent_Identity=26.2054507337526, Blast_Score=117, Evalue=3e-26,
Organism=Homo sapiens, GI58082049, Length=476, Percent_Identity=26.0504201680672, Blast_Score=112, Evalue=9e-25,
Organism=Homo sapiens, GI13325057, Length=371, Percent_Identity=28.3018867924528, Blast_Score=97, Evalue=5e-20,
Organism=Homo sapiens, GI195947378, Length=285, Percent_Identity=28.0701754385965, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI13375727, Length=372, Percent_Identity=24.7311827956989, Blast_Score=79, Evalue=8e-15,
Organism=Escherichia coli, GI1788107, Length=484, Percent_Identity=28.7190082644628, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI221142682, Length=459, Percent_Identity=26.5795206971678, Blast_Score=136, Evalue=3e-33,
Organism=Escherichia coli, GI145693145, Length=452, Percent_Identity=27.4336283185841, Blast_Score=134, Evalue=2e-32,
Organism=Escherichia coli, GI1786810, Length=373, Percent_Identity=26.2734584450402, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1789201, Length=362, Percent_Identity=26.5193370165746, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1790505, Length=368, Percent_Identity=23.9130434782609, Blast_Score=69, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17560308, Length=486, Percent_Identity=29.8353909465021, Blast_Score=176, Evalue=3e-44,
Organism=Caenorhabditis elegans, GI17558820, Length=464, Percent_Identity=29.0948275862069, Blast_Score=159, Evalue=3e-39,
Organism=Caenorhabditis elegans, GI32563687, Length=454, Percent_Identity=28.6343612334802, Blast_Score=156, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI17531443, Length=490, Percent_Identity=28.1632653061224, Blast_Score=150, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI71994694, Length=358, Percent_Identity=29.3296089385475, Blast_Score=149, Evalue=4e-36,
Organism=Caenorhabditis elegans, GI71994703, Length=358, Percent_Identity=29.3296089385475, Blast_Score=149, Evalue=5e-36,
Organism=Caenorhabditis elegans, GI71994690, Length=358, Percent_Identity=29.3296089385475, Blast_Score=148, Evalue=5e-36,
Organism=Caenorhabditis elegans, GI17559526, Length=356, Percent_Identity=30.8988764044944, Blast_Score=139, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17557194, Length=474, Percent_Identity=26.7932489451477, Blast_Score=119, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI71985884, Length=392, Percent_Identity=27.0408163265306, Blast_Score=104, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17538037, Length=480, Percent_Identity=26.6666666666667, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI115534440, Length=509, Percent_Identity=25.5402750491159, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI71996755, Length=351, Percent_Identity=25.3561253561254, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17560140, Length=344, Percent_Identity=24.4186046511628, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI25141359, Length=393, Percent_Identity=23.1552162849873, Blast_Score=79, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6319699, Length=501, Percent_Identity=26.1477045908184, Blast_Score=148, Evalue=2e-36,
Organism=Drosophila melanogaster, GI21355181, Length=471, Percent_Identity=26.1146496815287, Blast_Score=141, Evalue=8e-34,
Organism=Drosophila melanogaster, GI24581924, Length=483, Percent_Identity=27.536231884058, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI18859661, Length=479, Percent_Identity=26.9311064718163, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24648260, Length=349, Percent_Identity=26.9340974212034, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI24653035, Length=352, Percent_Identity=25, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI24656500, Length=357, Percent_Identity=25.4901960784314, Blast_Score=105, Evalue=5e-23,
Organism=Drosophila melanogaster, GI161076582, Length=364, Percent_Identity=23.3516483516484, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24648257, Length=480, Percent_Identity=23.125, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24648253, Length=424, Percent_Identity=24.5283018867925, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI281365686, Length=310, Percent_Identity=25.1612903225806, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24648255, Length=427, Percent_Identity=24.3559718969555, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI20130357, Length=201, Percent_Identity=29.8507462686567, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI19922652, Length=292, Percent_Identity=29.1095890410959, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI21355225, Length=368, Percent_Identity=24.7282608695652, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI21358303, Length=309, Percent_Identity=24.9190938511327, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI21356441, Length=311, Percent_Identity=24.4372990353698, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI21356947, Length=421, Percent_Identity=23.9904988123515, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24667959, Length=362, Percent_Identity=24.585635359116, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24667955, Length=362, Percent_Identity=24.585635359116, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI62472339, Length=362, Percent_Identity=24.585635359116, Blast_Score=86, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.3 [H]

Molecular weight: Translated: 51922; Mature: 51922

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLMRNDIAFIEAAYGAMRLGAYGVPVNWHFKPEEIDYILKDSATRVLIGHADLLHQLRGL
CCCCCCHHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHCCCCCCEEEEECHHHHHHHHCC
IPPGVTVLSVPTPPEILSNYKISADLLQPPEFAIELESWLVQHSPYEGPQVPQPQNMIYT
CCCCCEEEECCCCHHHHCCCCEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEEE
SGTTGHPKGVRRSAPTVEQAASAERMRALIYGLKPGSRALLPGPLYHSAPNAFGLRAGRL
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCCCCCCCCC
GGALVLMPRFEPEEFLSVIQSERIDTIFMVPTMFIRLMKLPEEVRRRFVVSSLRHVIHAA
CCEEEEEECCCHHHHHHHHHHCCCCEEEHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHC
APCPADVKRAMIEWWGPVIYEFYGSTESGAVTFATSEDALRKPGTVGKVSPGAELRFLGD
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECHHHHHCCCCCCCCCCCCEEEEECC
DGRILPQGEIGEIYSRIAGNPDFTYHNKPEKRAEIDRDGFITSGDVGYMDAEGYVFLCDR
CCCCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHCCCCCCCEECCCCCEEECCCEEEEECC
KRDMVISGGVNIYPAEIEAVLHAVPGVHDCAVFGVPDAEFGEALMAVVEPQPGVVLDTGE
CCCEEEECCCEEEHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHCCCCCCEEEECCC
IKLRLKASLADYKVPRQIEIQAKLPREDSGKIFKRRLRDPYWEQAGRKI
EEEEEEECHHCCCCCCEEEEEEECCCCCCHHHHHHHHCCHHHHHCCCCC
>Mature Secondary Structure
MLMRNDIAFIEAAYGAMRLGAYGVPVNWHFKPEEIDYILKDSATRVLIGHADLLHQLRGL
CCCCCCHHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHCCCCCCEEEEECHHHHHHHHCC
IPPGVTVLSVPTPPEILSNYKISADLLQPPEFAIELESWLVQHSPYEGPQVPQPQNMIYT
CCCCCEEEECCCCHHHHCCCCEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEEE
SGTTGHPKGVRRSAPTVEQAASAERMRALIYGLKPGSRALLPGPLYHSAPNAFGLRAGRL
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCCCCCCCCC
GGALVLMPRFEPEEFLSVIQSERIDTIFMVPTMFIRLMKLPEEVRRRFVVSSLRHVIHAA
CCEEEEEECCCHHHHHHHHHHCCCCEEEHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHC
APCPADVKRAMIEWWGPVIYEFYGSTESGAVTFATSEDALRKPGTVGKVSPGAELRFLGD
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECHHHHHCCCCCCCCCCCCEEEEECC
DGRILPQGEIGEIYSRIAGNPDFTYHNKPEKRAEIDRDGFITSGDVGYMDAEGYVFLCDR
CCCCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHCCCCCCCEECCCCCEEECCCEEEEECC
KRDMVISGGVNIYPAEIEAVLHAVPGVHDCAVFGVPDAEFGEALMAVVEPQPGVVLDTGE
CCCEEEECCCEEEHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHCCCCCCEEEECCC
IKLRLKASLADYKVPRQIEIQAKLPREDSGKIFKRRLRDPYWEQAGRKI
EEEEEEECHHCCCCCCEEEEEEECCCCCCHHHHHHHHCCHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]