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The map label for this gene is ygbJ [H]

Identifier: 146339560

GI number: 146339560

Start: 2724532

End: 2725443

Strand: Direct

Name: ygbJ [H]

Synonym: BRADO2551

Alternate gene names: 146339560

Gene position: 2724532-2725443 (Clockwise)

Preceding gene: 146339557

Following gene: 146339561

Centisome position: 36.54

GC content: 68.42

Gene sequence:

>912_bases
ATGTCGTCATCGCAATTGAACGTCGCCGTGATCGGGCTCGGCTCGATGGGCTATGGCATGGCGACCTCGCTCAAGCGCGC
CGGCTTCGTGGTGACCGGATGCGACGTCTCGGCGGATGCGGTGAAGCGCTTCGTCGCCGATGGCGGCAAGGAGGCTGACA
GCCCGGCCGAGGCCGCCGCGGGCGCCGACATCGTCGTGAGCGTCGTGGTCAATGCGGCGCAGACCGAAGCCATTCTGTTC
GGCCCTGGCGGCGCCGCGGAGACCATGCCGAAGGACTCGGTGTTCGTCTCGTCCGCGACCATGGACCCGGACGTGGCCCG
GCGTCTCGCCAAGCAGTTGGAGGCGACCGGCCGGCATTATCTCGACGCGCCGATCTCGGGTGGCGCGCAGCGCGCGGCGC
AGGGCGAACTGACGATCCTGGCCTCGGGCAGCGCGGCCGCGTTTGCCAAGGCGCGGCCTGCGCTCGATGCGATGGCGGCG
AAGCTCTACGAGCTCGGCGATGCCGCGGGCCAGGGGGCCGCGTTCAAGATGATCAACCAGCTGCTTGCCGGCGTGCACAT
CGCCGCCGCCAGCGAAGCGATCGCGTTCGCAGCCAAGCAGGGCCTCGATATCAGGAAGGTCTACGAGGTCATCACCGCCT
CGGCCGGCAATTCCTGGATGTTCGAAAACCGCATGCCGCATGTGCTCGACGGCGACTACACGCCGCGCAGCGCGGTCGAG
ATCTTCGTCAAGGACCTCGGCATCATCCAGGACATGGCGCGCAGTCATCGCTTCCCCGTGCCGGTCTCGGCCGCCGCGCT
GCAGATGTTCCTGATGACGGCAGCCGCCGGCATGGGCCGCGACGACGACGCCTCGGTGGCGCGCATGTATGCCCAGGTCA
CCGGCACCACGCTGCCCGGCTCATCCAAGTAA

Upstream 100 bases:

>100_bases
CGAGCTTTGTCTCACGCTTTGGCAGCGCGGCCAAGCCGTGTTACCCAGGGACGAGCCGGGTTAACCGGACAGGTCGGGAA
ACGCATCGAGGATTTGCTGC

Downstream 100 bases:

>100_bases
CAGCACGAGTCCTCGCATCATGCCTCGTTTCGCCGCCAATCTCTCGATGATGTTCACCGAGCATCCCTTCCTCGATCGCT
TCGCGGCCGCCGCCAAGGCC

Product: dehydrogenase NAD(P)-binding domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 303; Mature: 302

Protein sequence:

>303_residues
MSSSQLNVAVIGLGSMGYGMATSLKRAGFVVTGCDVSADAVKRFVADGGKEADSPAEAAAGADIVVSVVVNAAQTEAILF
GPGGAAETMPKDSVFVSSATMDPDVARRLAKQLEATGRHYLDAPISGGAQRAAQGELTILASGSAAAFAKARPALDAMAA
KLYELGDAAGQGAAFKMINQLLAGVHIAAASEAIAFAAKQGLDIRKVYEVITASAGNSWMFENRMPHVLDGDYTPRSAVE
IFVKDLGIIQDMARSHRFPVPVSAAALQMFLMTAAAGMGRDDDASVARMYAQVTGTTLPGSSK

Sequences:

>Translated_303_residues
MSSSQLNVAVIGLGSMGYGMATSLKRAGFVVTGCDVSADAVKRFVADGGKEADSPAEAAAGADIVVSVVVNAAQTEAILF
GPGGAAETMPKDSVFVSSATMDPDVARRLAKQLEATGRHYLDAPISGGAQRAAQGELTILASGSAAAFAKARPALDAMAA
KLYELGDAAGQGAAFKMINQLLAGVHIAAASEAIAFAAKQGLDIRKVYEVITASAGNSWMFENRMPHVLDGDYTPRSAVE
IFVKDLGIIQDMARSHRFPVPVSAAALQMFLMTAAAGMGRDDDASVARMYAQVTGTTLPGSSK
>Mature_302_residues
SSSQLNVAVIGLGSMGYGMATSLKRAGFVVTGCDVSADAVKRFVADGGKEADSPAEAAAGADIVVSVVVNAAQTEAILFG
PGGAAETMPKDSVFVSSATMDPDVARRLAKQLEATGRHYLDAPISGGAQRAAQGELTILASGSAAAFAKARPALDAMAAK
LYELGDAAGQGAAFKMINQLLAGVHIAAASEAIAFAAKQGLDIRKVYEVITASAGNSWMFENRMPHVLDGDYTPRSAVEI
FVKDLGIIQDMARSHRFPVPVSAAALQMFLMTAAAGMGRDDDASVARMYAQVTGTTLPGSSK

Specific function: Unknown

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308751, Length=291, Percent_Identity=32.3024054982818, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI40556376, Length=283, Percent_Identity=24.7349823321555, Blast_Score=114, Evalue=1e-25,
Organism=Escherichia coli, GI1789092, Length=302, Percent_Identity=46.3576158940397, Blast_Score=248, Evalue=5e-67,
Organism=Escherichia coli, GI145693186, Length=288, Percent_Identity=28.4722222222222, Blast_Score=137, Evalue=6e-34,
Organism=Escherichia coli, GI1790315, Length=280, Percent_Identity=31.0714285714286, Blast_Score=135, Evalue=2e-33,
Organism=Escherichia coli, GI1786719, Length=285, Percent_Identity=28.0701754385965, Blast_Score=122, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI17557316, Length=272, Percent_Identity=29.4117647058824, Blast_Score=109, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24655230, Length=283, Percent_Identity=30.3886925795053, Blast_Score=141, Evalue=5e-34,
Organism=Drosophila melanogaster, GI19922568, Length=283, Percent_Identity=30.3886925795053, Blast_Score=141, Evalue=5e-34,
Organism=Drosophila melanogaster, GI28574115, Length=284, Percent_Identity=23.943661971831, Blast_Score=107, Evalue=9e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: 1.1.-.- [C]

Molecular weight: Translated: 31120; Mature: 30989

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: PS00895 3_HYDROXYISOBUT_DH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSSQLNVAVIGLGSMGYGMATSLKRAGFVVTGCDVSADAVKRFVADGGKEADSPAEAAA
CCCCCEEEEEEEECCCCCHHHHHHHHCCEEEECCCCCHHHHHHHHHCCCCCCCCCHHHHC
GADIVVSVVVNAAQTEAILFGPGGAAETMPKDSVFVSSATMDPDVARRLAKQLEATGRHY
CHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCEE
LDAPISGGAQRAAQGELTILASGSAAAFAKARPALDAMAAKLYELGDAAGQGAAFKMINQ
ECCCCCCCHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
LLAGVHIAAASEAIAFAAKQGLDIRKVYEVITASAGNSWMFENRMPHVLDGDYTPRSAVE
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCHHHHH
IFVKDLGIIQDMARSHRFPVPVSAAALQMFLMTAAAGMGRDDDASVARMYAQVTGTTLPG
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCC
SSK
CCC
>Mature Secondary Structure 
SSSQLNVAVIGLGSMGYGMATSLKRAGFVVTGCDVSADAVKRFVADGGKEADSPAEAAA
CCCCEEEEEEEECCCCCHHHHHHHHCCEEEECCCCCHHHHHHHHHCCCCCCCCCHHHHC
GADIVVSVVVNAAQTEAILFGPGGAAETMPKDSVFVSSATMDPDVARRLAKQLEATGRHY
CHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCEE
LDAPISGGAQRAAQGELTILASGSAAAFAKARPALDAMAAKLYELGDAAGQGAAFKMINQ
ECCCCCCCHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
LLAGVHIAAASEAIAFAAKQGLDIRKVYEVITASAGNSWMFENRMPHVLDGDYTPRSAVE
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCHHHHH
IFVKDLGIIQDMARSHRFPVPVSAAALQMFLMTAAAGMGRDDDASVARMYAQVTGTTLPG
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCC
SSK
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]