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The map label for this gene is thcD [H]

Identifier: 146339502

GI number: 146339502

Start: 2659378

End: 2660613

Strand: Direct

Name: thcD [H]

Synonym: BRADO2491

Alternate gene names: 146339502

Gene position: 2659378-2660613 (Clockwise)

Preceding gene: 146339500

Following gene: 146339505

Centisome position: 35.66

GC content: 65.86

Gene sequence:

>1236_bases
ATGGGGCAGGGTGCTGTGACTAAGAGACCGGTGGTGATCGTGGGTGCGGGGCATGCGGGCTTTCAGCTCGCGACCTCGTT
GCGTCAGGCAGGCTTTGTCGATCCCATTCATCTGATCAACGACGAGCCGCATCTGCCGTATCAGCGCCCGCCTTTGTCGA
AGGCCTATCTCAAGGGTACCGGCGGCCCCGAGACGCTGATGTTTCGGCCGCAGAAGTTTTACGCCGACCAGGCCGTCGAT
CTGGTCTATGACCGCGCCGTGGCGGTGCAGCGTGAGCCGCGCAAGGTGCTGCTGGCCTCCGGCAAGACCCTGGACTATGG
CCACCTCGTGTTCGCGACCGGCGCGCGCAACCGACTGCTCGATATTCCCAACGCCAATCTTCCGGCGGTTCGTTACCTCC
GCATCCTCGATGACAGCGAAGCGTTGCGCAGCCTGCTTGGCGACGCCAAGCGCGTCGTCGTGATCGGCGCCGGCTTCATC
GGTCTTGAATTCGCTGCCACCGCGCGCATCAAGGGACTCGAGGTCGACGTGCTCGAACTCGGCGCGCGGGTGATGGCGCG
GGCGGTGACGGCGGAAATCTCCGAGTACTTCCAGAAGCAGCATGCCGATGCCGGCGTACGCATCCATCTCGGCGTGCAGT
CCACCAGCATCGAGGCCGACGGCAACAAGGTCATCGGCGTCAGCCTCAGCGACGGTCGGCATATTCCCGCCGATCTCGTC
GTGGTCGGCGTCGGCGTGCTGCCGAATGTCGAGCTCGCGGCGGAGGCCGGATTGCAGGTCGCCTCCGGCATCGTCGTCGA
CGAATATCTCCTGACCAGCGATCCGCACATCTCGGCGATCGGCGACTGCGCGCTGTTCGCCAGCCCGCGCTTCGGCGGGA
CCCTGCGGCTGGAGTCGGTACAGAACGCGACCGACCATGCCCGCTGCGTCGCCGCGCGGCTCACGGGAGACGTCAAGCCG
TACGATGGCCAGCCCTGGTTCTGGAGCGACCAGGCCAATGACAAGCTCCAGATCGCCGGGCTCACCACGGGCTATGACCA
GGTCGTGCTGCGCGGGGATCCGGCGCAAAAGGCATTCTCCGCATTCTGCTACAAAGAGGGCCGGCTGGTCGGCATCGAAT
CCGTCAACCGCGCCGGTGATCACATGTTCGGCCGCAAGCTGCTCGCCGCGGGCGGCACGATCGATCCGGCCGAGGCCGCC
GATCCGAGCTTCGACCTGAAGAGCGCGCTCGGCTAG

Upstream 100 bases:

>100_bases
GGTGACAATGCCGGGGAAGTCTGCGACAACCCGGTTCCGCTGGCATGACGGGAGGTAAACCCGGAGCAGACGAAGCGCGC
GAACACGGGATACGGACAGA

Downstream 100 bases:

>100_bases
AGCAGCGCGCGCACCTCCTCTGCTGTGGGCATCGATGGGCCGGCGCCCATCCGCTGCACGGAGATCGACGCGGCGACATT
GGCGTAGGCGATCGCATCCG

Product: putative ferredoxin--NAD(+) reductase

Products: oxidized ferredoxin; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 411; Mature: 410

Protein sequence:

>411_residues
MGQGAVTKRPVVIVGAGHAGFQLATSLRQAGFVDPIHLINDEPHLPYQRPPLSKAYLKGTGGPETLMFRPQKFYADQAVD
LVYDRAVAVQREPRKVLLASGKTLDYGHLVFATGARNRLLDIPNANLPAVRYLRILDDSEALRSLLGDAKRVVVIGAGFI
GLEFAATARIKGLEVDVLELGARVMARAVTAEISEYFQKQHADAGVRIHLGVQSTSIEADGNKVIGVSLSDGRHIPADLV
VVGVGVLPNVELAAEAGLQVASGIVVDEYLLTSDPHISAIGDCALFASPRFGGTLRLESVQNATDHARCVAARLTGDVKP
YDGQPWFWSDQANDKLQIAGLTTGYDQVVLRGDPAQKAFSAFCYKEGRLVGIESVNRAGDHMFGRKLLAAGGTIDPAEAA
DPSFDLKSALG

Sequences:

>Translated_411_residues
MGQGAVTKRPVVIVGAGHAGFQLATSLRQAGFVDPIHLINDEPHLPYQRPPLSKAYLKGTGGPETLMFRPQKFYADQAVD
LVYDRAVAVQREPRKVLLASGKTLDYGHLVFATGARNRLLDIPNANLPAVRYLRILDDSEALRSLLGDAKRVVVIGAGFI
GLEFAATARIKGLEVDVLELGARVMARAVTAEISEYFQKQHADAGVRIHLGVQSTSIEADGNKVIGVSLSDGRHIPADLV
VVGVGVLPNVELAAEAGLQVASGIVVDEYLLTSDPHISAIGDCALFASPRFGGTLRLESVQNATDHARCVAARLTGDVKP
YDGQPWFWSDQANDKLQIAGLTTGYDQVVLRGDPAQKAFSAFCYKEGRLVGIESVNRAGDHMFGRKLLAAGGTIDPAEAA
DPSFDLKSALG
>Mature_410_residues
GQGAVTKRPVVIVGAGHAGFQLATSLRQAGFVDPIHLINDEPHLPYQRPPLSKAYLKGTGGPETLMFRPQKFYADQAVDL
VYDRAVAVQREPRKVLLASGKTLDYGHLVFATGARNRLLDIPNANLPAVRYLRILDDSEALRSLLGDAKRVVVIGAGFIG
LEFAATARIKGLEVDVLELGARVMARAVTAEISEYFQKQHADAGVRIHLGVQSTSIEADGNKVIGVSLSDGRHIPADLVV
VGVGVLPNVELAAEAGLQVASGIVVDEYLLTSDPHISAIGDCALFASPRFGGTLRLESVQNATDHARCVAARLTGDVKPY
DGQPWFWSDQANDKLQIAGLTTGYDQVVLRGDPAQKAFSAFCYKEGRLVGIESVNRAGDHMFGRKLLAAGGTIDPAEAAD
PSFDLKSALG

Specific function: The degradation of the thiocarbamate herbicide EPTC by cytochrome CYP116 (thcB) requires the participation of a flavoprotein, rhodocoxin reductase, and an iron-sulfur protein, rhodocoxin, to mediate the transfer of electrons from NADH to P450 for oxygen a

COG id: COG0446

COG function: function code R; Uncharacterized NAD(FAD)-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI21389617, Length=370, Percent_Identity=28.9189189189189, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI226437568, Length=370, Percent_Identity=28.9189189189189, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI65787454, Length=370, Percent_Identity=28.9189189189189, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI4757732, Length=335, Percent_Identity=28.3582089552239, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI22202629, Length=335, Percent_Identity=28.3582089552239, Blast_Score=106, Evalue=4e-23,
Organism=Homo sapiens, GI22202631, Length=170, Percent_Identity=31.1764705882353, Blast_Score=66, Evalue=7e-11,
Organism=Escherichia coli, GI1788892, Length=406, Percent_Identity=37.4384236453202, Blast_Score=209, Evalue=2e-55,
Organism=Escherichia coli, GI1789065, Length=274, Percent_Identity=29.1970802919708, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI1789765, Length=294, Percent_Identity=29.2517006802721, Blast_Score=104, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17559934, Length=410, Percent_Identity=26.3414634146341, Blast_Score=132, Evalue=4e-31,
Organism=Drosophila melanogaster, GI24639257, Length=408, Percent_Identity=27.2058823529412, Blast_Score=112, Evalue=3e-25,
Organism=Drosophila melanogaster, GI281359715, Length=408, Percent_Identity=27.2058823529412, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI281359713, Length=408, Percent_Identity=27.2058823529412, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI24639250, Length=408, Percent_Identity=27.2058823529412, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI18543267, Length=408, Percent_Identity=27.2058823529412, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI24639252, Length=408, Percent_Identity=27.2058823529412, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI24581020, Length=360, Percent_Identity=26.9444444444444, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI28573993, Length=360, Percent_Identity=26.9444444444444, Blast_Score=109, Evalue=4e-24,
Organism=Drosophila melanogaster, GI24585130, Length=407, Percent_Identity=22.8501228501229, Blast_Score=98, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR004099
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.18.1.3

Molecular weight: Translated: 43838; Mature: 43706

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGQGAVTKRPVVIVGAGHAGFQLATSLRQAGFVDPIHLINDEPHLPYQRPPLSKAYLKGT
CCCCCCCCCCEEEEECCCCHHHHHHHHHHCCCCCCHHEECCCCCCCCCCCCCCHHEEECC
GGPETLMFRPQKFYADQAVDLVYDRAVAVQREPRKVLLASGKTLDYGHLVFATGARNRLL
CCCCEEEECCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCEECCCEEEEEECCCCCEE
DIPNANLPAVRYLRILDDSEALRSLLGDAKRVVVIGAGFIGLEFAATARIKGLEVDVLEL
ECCCCCCCCEEEEEEECCHHHHHHHHCCCCEEEEEECCHHHEEEEEEEEECCCEEHHHHH
GARVMARAVTAEISEYFQKQHADAGVRIHLGVQSTSIEADGNKVIGVSLSDGRHIPADLV
HHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEEEECCCCEEEEEEECCCCCCCCEEE
VVGVGVLPNVELAAEAGLQVASGIVVDEYLLTSDPHISAIGDCALFASPRFGGTLRLESV
EEECCCCCCCHHHHHCCCHHHCCEEEEHEEECCCCCCCEECCEEEEECCCCCCEEEECCC
QNATDHARCVAARLTGDVKPYDGQPWFWSDQANDKLQIAGLTTGYDQVVLRGDPAQKAFS
CCCCHHHHHEEEECCCCCCCCCCCCEEECCCCCCEEEEEEEECCCCEEEEECCCHHHHHH
AFCYKEGRLVGIESVNRAGDHMFGRKLLAAGGTIDPAEAADPSFDLKSALG
HHHHCCCCEEEHHHHCCCCHHHHHHHHHHCCCCCCCHHCCCCCCCHHHHCC
>Mature Secondary Structure 
GQGAVTKRPVVIVGAGHAGFQLATSLRQAGFVDPIHLINDEPHLPYQRPPLSKAYLKGT
CCCCCCCCCEEEEECCCCHHHHHHHHHHCCCCCCHHEECCCCCCCCCCCCCCHHEEECC
GGPETLMFRPQKFYADQAVDLVYDRAVAVQREPRKVLLASGKTLDYGHLVFATGARNRLL
CCCCEEEECCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCEECCCEEEEEECCCCCEE
DIPNANLPAVRYLRILDDSEALRSLLGDAKRVVVIGAGFIGLEFAATARIKGLEVDVLEL
ECCCCCCCCEEEEEEECCHHHHHHHHCCCCEEEEEECCHHHEEEEEEEEECCCEEHHHHH
GARVMARAVTAEISEYFQKQHADAGVRIHLGVQSTSIEADGNKVIGVSLSDGRHIPADLV
HHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEEEECCCCEEEEEEECCCCCCCCEEE
VVGVGVLPNVELAAEAGLQVASGIVVDEYLLTSDPHISAIGDCALFASPRFGGTLRLESV
EEECCCCCCCHHHHHCCCHHHCCEEEEHEEECCCCCCCEECCEEEEECCCCCCEEEECCC
QNATDHARCVAARLTGDVKPYDGQPWFWSDQANDKLQIAGLTTGYDQVVLRGDPAQKAFS
CCCCHHHHHEEEECCCCCCCCCCCCEEECCCCCCEEEEEEEECCCCEEEEECCCHHHHHH
AFCYKEGRLVGIESVNRAGDHMFGRKLLAAGGTIDPAEAADPSFDLKSALG
HHHHCCCCEEEHHHHCCCCHHHHHHHHHHCCCCCCCHHCCCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced ferredoxin; NAD+

Specific reaction: reduced ferredoxin + NAD+ = oxidized ferredoxin + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7836301 [H]