Click here to switch to the map view.

The map label for this gene is tfdB [H]

Identifier: 146339295

GI number: 146339295

Start: 2419429

End: 2421183

Strand: Direct

Name: tfdB [H]

Synonym: BRADO2271

Alternate gene names: 146339295

Gene position: 2419429-2421183 (Clockwise)

Preceding gene: 146339294

Following gene: 146339296

Centisome position: 32.45

GC content: 65.64

Gene sequence:

>1755_bases
ATGGCCGACATCACGACAGACGTTCTCATCATCGGTACCGGTCCGGCCGGCTCGGCCGCGGCGGCGCTGCTGTCCACCTA
TGGCGTCGAGAACATGGTGGTCAACCGCTATCGCTGGCTCGCCAACACGCCGCGCGCCCACATCACCAACCAGCGCACGA
TGGAGGTGCTGCGCGACCTTGGCCGCGACGTCGAGAACGAAGCCTACATGTTCGCCTCGCAGCAGGACATCATGGGCGAG
AACGTGTTCTGCACCTCGCTCGCCGGCGAGGAGATCGGCCGGCTCAAGACCTGGGGCAATCACCCGCTGTCGCGCGCCGA
GCATCAGCTGTCGTCGCCGGCGAAGATGAACGATCTGCCGCAGACCTATATGGAGCCGCTGCTGTTCAAGACCGCATGCT
CGCGCGGCACGCAGGCGCGGATGTCCACGGTGTATGAGAGCCACGTTCAGGATGATCACGGCGTCACCACGACCTTGCTC
GATCGTCTGACGGGCAAGACCTTCACGGTGCGCTCGAAATATCTGATCGGCGCCGACGGCGGCAACTCGCTGGTCGCCCA
GCATGCCGGCCTCCCATTCGAGGGCAAGATGGGTGTCGGCGGCTCGATGAACATCCTGTTCAAGGCCGATCTGTCGCGCT
ACGTCGCCCACCGGCCGTCGGTGCTGTATTGGGTGGTGCAGCCGGGCGCCGATGTCGGCGGCATCGGCATGGGGCTGGTG
CGCATGGTCAGGCCGTGGAACGAATGGCTGATCGTGTGGGGCTATGACATCAACCAGCCGGCGCCGGAGGTCGACGAGGC
CTTTGCGGTGAAGGTCGCGCGGGATCTCGTCGGCGATCAGGAGCTGCAGATCGAGCTGCAGTCGGTGTCGACCTGGACCG
TCAACAACATGTATGCGACGCGGACGTCGCATGGCCGCGTATTCTGCATGGGCGATGCGATCCACCGGCATCCGCCGTCG
AATGGTCTCGGCTCGAACACCTCGATCCAGGACGCCTTCAACCTGGCCTGGAAGCTCGCTTACGTCCTCAAGGACCGCGC
CGGGCCAAAGCTGCTCGACAGCTACACGATCGAGCGCGCCCCGGTCGCCAAGCAGATCGTCACCCGCGCCAACAAGTCGA
TCGAGGAGTTCGGCCCGATCTTCCAGGCGCTAGGGCTGCTGGACTCGGTTGATCCCGTGAAGATGCAGCAGAACATGGAC
GCGCGCTGCGACCGCACCGAGCAGGCCGAGCAGCAGCGCGCGAAGATCCGCGAGACGATCGCCAGCAAGGTCTATGAGTT
CGACGCGCACGGCGTCGAGATGAACCACCGCTATCGCTCGGACGCCATCGTGACCGATGGGCAGGCCGAGCCGGCCTTCA
CCCGGGATCCCGAGCTGCATTTCCAGCAGACGACCTGGCCCGGCGCGCGGCTGCCGCATGCCTGGCTGTTCGGCAAGGAC
GGCACCAAGATCTCCACGCTCGACCTTGCCGGTCATGGCAAGTTCACGGTGCTGACCGGCATCGGCGGTGAGACCTGGGT
GCAGGCCGCCAGGAAGCTCGGCCAGGAGCTCGGCATCGAGATCGATGCGCATGTCATCGGCCCGCGCCAGGTCTGGCAGG
ATCTCGACGGCGACTGGTCGCGGTTGCGCGAGGTGCGGGACGGCGGCATCGTGCTGACTCGGCCCGATCAGCACGTCTGC
TGGCGGCGCGAGACCTTGGCTGAGGATCCGGCCGCGGAACTGCGCCGCGTGTTCAAGCAAATCCTGGCGAACTGA

Upstream 100 bases:

>100_bases
TTCGCAAGCCGGACCGGCATCCAGCTGCTGCCGGTCCGCCGCCTGCTGCGCGGCGGATCAATCGACAACTGACAAGACGA
CAAACGACAGGGAGGGCCCG

Downstream 100 bases:

>100_bases
AGCCGAAGGGGAAGACCTCATGGATGATCATGAGCAGGGCTATTTCACCGAAGAGAACTCCGCCGAGGTCGTGATCGGCC
GCAACAAGAACGCCAAGGAC

Product: 2,4-dichlorophenol 6-monooxygenase

Products: NA

Alternate protein names: 2,4-dichlorophenol hydroxylase; 2,4-DCP hydroxylase [H]

Number of amino acids: Translated: 584; Mature: 583

Protein sequence:

>584_residues
MADITTDVLIIGTGPAGSAAAALLSTYGVENMVVNRYRWLANTPRAHITNQRTMEVLRDLGRDVENEAYMFASQQDIMGE
NVFCTSLAGEEIGRLKTWGNHPLSRAEHQLSSPAKMNDLPQTYMEPLLFKTACSRGTQARMSTVYESHVQDDHGVTTTLL
DRLTGKTFTVRSKYLIGADGGNSLVAQHAGLPFEGKMGVGGSMNILFKADLSRYVAHRPSVLYWVVQPGADVGGIGMGLV
RMVRPWNEWLIVWGYDINQPAPEVDEAFAVKVARDLVGDQELQIELQSVSTWTVNNMYATRTSHGRVFCMGDAIHRHPPS
NGLGSNTSIQDAFNLAWKLAYVLKDRAGPKLLDSYTIERAPVAKQIVTRANKSIEEFGPIFQALGLLDSVDPVKMQQNMD
ARCDRTEQAEQQRAKIRETIASKVYEFDAHGVEMNHRYRSDAIVTDGQAEPAFTRDPELHFQQTTWPGARLPHAWLFGKD
GTKISTLDLAGHGKFTVLTGIGGETWVQAARKLGQELGIEIDAHVIGPRQVWQDLDGDWSRLREVRDGGIVLTRPDQHVC
WRRETLAEDPAAELRRVFKQILAN

Sequences:

>Translated_584_residues
MADITTDVLIIGTGPAGSAAAALLSTYGVENMVVNRYRWLANTPRAHITNQRTMEVLRDLGRDVENEAYMFASQQDIMGE
NVFCTSLAGEEIGRLKTWGNHPLSRAEHQLSSPAKMNDLPQTYMEPLLFKTACSRGTQARMSTVYESHVQDDHGVTTTLL
DRLTGKTFTVRSKYLIGADGGNSLVAQHAGLPFEGKMGVGGSMNILFKADLSRYVAHRPSVLYWVVQPGADVGGIGMGLV
RMVRPWNEWLIVWGYDINQPAPEVDEAFAVKVARDLVGDQELQIELQSVSTWTVNNMYATRTSHGRVFCMGDAIHRHPPS
NGLGSNTSIQDAFNLAWKLAYVLKDRAGPKLLDSYTIERAPVAKQIVTRANKSIEEFGPIFQALGLLDSVDPVKMQQNMD
ARCDRTEQAEQQRAKIRETIASKVYEFDAHGVEMNHRYRSDAIVTDGQAEPAFTRDPELHFQQTTWPGARLPHAWLFGKD
GTKISTLDLAGHGKFTVLTGIGGETWVQAARKLGQELGIEIDAHVIGPRQVWQDLDGDWSRLREVRDGGIVLTRPDQHVC
WRRETLAEDPAAELRRVFKQILAN
>Mature_583_residues
ADITTDVLIIGTGPAGSAAAALLSTYGVENMVVNRYRWLANTPRAHITNQRTMEVLRDLGRDVENEAYMFASQQDIMGEN
VFCTSLAGEEIGRLKTWGNHPLSRAEHQLSSPAKMNDLPQTYMEPLLFKTACSRGTQARMSTVYESHVQDDHGVTTTLLD
RLTGKTFTVRSKYLIGADGGNSLVAQHAGLPFEGKMGVGGSMNILFKADLSRYVAHRPSVLYWVVQPGADVGGIGMGLVR
MVRPWNEWLIVWGYDINQPAPEVDEAFAVKVARDLVGDQELQIELQSVSTWTVNNMYATRTSHGRVFCMGDAIHRHPPSN
GLGSNTSIQDAFNLAWKLAYVLKDRAGPKLLDSYTIERAPVAKQIVTRANKSIEEFGPIFQALGLLDSVDPVKMQQNMDA
RCDRTEQAEQQRAKIRETIASKVYEFDAHGVEMNHRYRSDAIVTDGQAEPAFTRDPELHFQQTTWPGARLPHAWLFGKDG
TKISTLDLAGHGKFTVLTGIGGETWVQAARKLGQELGIEIDAHVIGPRQVWQDLDGDWSRLREVRDGGIVLTRPDQHVCW
RRETLAEDPAAELRRVFKQILAN

Specific function: Transforms 2,4-dichlorophenol (2,4-DCP) into 3,5- dichlorocatechol [H]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pheA/tfdB FAD monooxygenase family [H]

Homologues:

Organism=Escherichia coli, GI1786543, Length=370, Percent_Identity=25.6756756756757, Blast_Score=80, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: =1.14.13.20 [H]

Molecular weight: Translated: 64961; Mature: 64830

Theoretical pI: Translated: 6.56; Mature: 6.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADITTDVLIIGTGPAGSAAAALLSTYGVENMVVNRYRWLANTPRAHITNQRTMEVLRDL
CCCCCCCEEEEECCCCCHHHHHHHHHHCHHHHHHHHHHHHCCCCCHHCCCHHHHHHHHHH
GRDVENEAYMFASQQDIMGENVFCTSLAGEEIGRLKTWGNHPLSRAEHQLSSPAKMNDLP
CCCCCCCCEEEECCHHHCCCCEEEEECCHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCC
QTYMEPLLFKTACSRGTQARMSTVYESHVQDDHGVTTTLLDRLTGKTFTVRSKYLIGADG
HHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCEEEEEEEEEEECCC
GNSLVAQHAGLPFEGKMGVGGSMNILFKADLSRYVAHRPSVLYWVVQPGADVGGIGMGLV
CCCHHHHHCCCCCCCCCCCCCCEEEEEECHHHHHHHCCCCEEEEEECCCCCCCHHHHHHH
RMVRPWNEWLIVWGYDINQPAPEVDEAFAVKVARDLVGDQELQIELQSVSTWTVNNMYAT
HHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEEECEEEEECCEEEE
RTSHGRVFCMGDAIHRHPPSNGLGSNTSIQDAFNLAWKLAYVLKDRAGPKLLDSYTIERA
ECCCCEEEEECCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCC
PVAKQIVTRANKSIEEFGPIFQALGLLDSVDPVKMQQNMDARCDRTEQAEQQRAKIRETI
HHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHH
ASKVYEFDAHGVEMNHRYRSDAIVTDGQAEPAFTRDPELHFQQTTWPGARLPHAWLFGKD
HHHHHHCCCCCCEECCCCCCCCEEECCCCCCCCCCCCCCCEEECCCCCCCCCCEEEECCC
GTKISTLDLAGHGKFTVLTGIGGETWVQAARKLGQELGIEIDAHVIGPRQVWQDLDGDWS
CCEEEEEEECCCCCEEEEECCCCHHHHHHHHHHHHHHCCEEEEEEECHHHHHHCCCCCHH
RLREVRDGGIVLTRPDQHVCWRRETLAEDPAAELRRVFKQILAN
HHHHHCCCCEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ADITTDVLIIGTGPAGSAAAALLSTYGVENMVVNRYRWLANTPRAHITNQRTMEVLRDL
CCCCCCEEEEECCCCCHHHHHHHHHHCHHHHHHHHHHHHCCCCCHHCCCHHHHHHHHHH
GRDVENEAYMFASQQDIMGENVFCTSLAGEEIGRLKTWGNHPLSRAEHQLSSPAKMNDLP
CCCCCCCCEEEECCHHHCCCCEEEEECCHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCC
QTYMEPLLFKTACSRGTQARMSTVYESHVQDDHGVTTTLLDRLTGKTFTVRSKYLIGADG
HHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCEEEEEEEEEEECCC
GNSLVAQHAGLPFEGKMGVGGSMNILFKADLSRYVAHRPSVLYWVVQPGADVGGIGMGLV
CCCHHHHHCCCCCCCCCCCCCCEEEEEECHHHHHHHCCCCEEEEEECCCCCCCHHHHHHH
RMVRPWNEWLIVWGYDINQPAPEVDEAFAVKVARDLVGDQELQIELQSVSTWTVNNMYAT
HHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEEECEEEEECCEEEE
RTSHGRVFCMGDAIHRHPPSNGLGSNTSIQDAFNLAWKLAYVLKDRAGPKLLDSYTIERA
ECCCCEEEEECCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCC
PVAKQIVTRANKSIEEFGPIFQALGLLDSVDPVKMQQNMDARCDRTEQAEQQRAKIRETI
HHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHH
ASKVYEFDAHGVEMNHRYRSDAIVTDGQAEPAFTRDPELHFQQTTWPGARLPHAWLFGKD
HHHHHHCCCCCCEECCCCCCCCEEECCCCCCCCCCCCCCCEEECCCCCCCCCCEEEECCC
GTKISTLDLAGHGKFTVLTGIGGETWVQAARKLGQELGIEIDAHVIGPRQVWQDLDGDWS
CCEEEEEEECCCCCEEEEECCCCHHHHHHHHHHHHHHCCEEEEEEECHHHHHHCCCCCHH
RLREVRDGGIVLTRPDQHVCWRRETLAEDPAAELRRVFKQILAN
HHHHHCCCCEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12949179; 11572451 [H]