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The map label for this gene is livM [H]

Identifier: 146339294

GI number: 146339294

Start: 2418339

End: 2419400

Strand: Direct

Name: livM [H]

Synonym: BRADO2270

Alternate gene names: 146339294

Gene position: 2418339-2419400 (Clockwise)

Preceding gene: 146339293

Following gene: 146339295

Centisome position: 32.43

GC content: 67.04

Gene sequence:

>1062_bases
ATGCACAGACGATCCATCCCCTGGCGTGTCGAGACGAGGACCAAGGCCTCGATTGTGTTTGCCGTGATCGCGGCGCTCAT
CGTCGTGGCCGGCGTGATCGCGCCGGCCTATCTGCCGCGCTCGACCTTGCAGGACCTGTTCTTCATCCTGACCATGCTGG
TGCTGGCGCAGAGCTGGAATCTGCTCGCGGGCTATGCCGGTCTCGTCTCCGTCGGCCAGCAGGCCTTCGTCGGCCTCGGC
GCCTACGCGATGTTCGCGGGCGTCGTGCTGCTGAAGCTCGATCCGCTGGTCTCAGTCCTGCTCGGCGGCGCCGCCGCCGC
ACTGCTCGCCATTCCCGCCGGCTTTTTCGCCTTCCGTCTGCAGGGCGCTTACTTCGCGATCGGCACCTGGGTGGTCGCGG
AGATCGTGCGACTGCTCGTCGCGCAGTGGAAGACGCTCGGCGGCGGCACTGGCACCTCGCTGCCGGGCGCGGCGACTCGC
GCGATGTTCGGCACCGATGTCGTGCAGCGTCTGCTCGGCGTGCGGCCGGCGCAGGCGGTCGACATCATCTGCTACTGGCT
GGCGCTGCTGCTTGCGGTCGCGACGATCTTCACGGTGTATCGCCTGTTGCGCTCGCGCCATGGTCTCGCGCTCGCCTCCG
TGCGCGACAATCAGGAGGCGGCGCGCGCCATCGGTGTCGATGCGCGGCGGCTGAAGGCGATGGTCTATCTGACCAGCGCC
GCGCTGACCGGGCTCGTCGGCGCGCTGATCTATGTGCAGAAGGCGCGGATCTCGCCGGACGCGGCGTTCTCGGTGATCGA
CTGGACGGCCTACGTCATCTTCATCGTGGTGATCGGCGGCATCGGCACCATCGAGGGGCCGATCGTCGGCGTCCTGATCT
TCTTCGGCCTGCAGAAGCTGCTGGCCGATTTCGGCTCATTGTATCTGCTCGCGCTCGGCCTGATCGGTATCGTCATCATG
CTGTTCGCGCCGCAAGGGTTATGGGGCCTGTTCGCAAGCCGGACCGGCATCCAGCTGCTGCCGGTCCGCCGCCTGCTGCG
CGGCGGATCAATCGACAACTGA

Upstream 100 bases:

>100_bases
CGGAATGGCAGATCCTGGCCGGTCACATCGCGTTCGTGCTGGTGCTGCTGGTCCGTCCGCGCGGCTTCTTCCCGCGCGCG
GTCGATTGAGGGGCAGGGCG

Downstream 100 bases:

>100_bases
CAAGACGACAAACGACAGGGAGGGCCCGATGGCCGACATCACGACAGACGTTCTCATCATCGGTACCGGTCCGGCCGGCT
CGGCCGCGGCGGCGCTGCTG

Product: putative ABC transporter permease

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: LIV-I protein M [H]

Number of amino acids: Translated: 353; Mature: 353

Protein sequence:

>353_residues
MHRRSIPWRVETRTKASIVFAVIAALIVVAGVIAPAYLPRSTLQDLFFILTMLVLAQSWNLLAGYAGLVSVGQQAFVGLG
AYAMFAGVVLLKLDPLVSVLLGGAAAALLAIPAGFFAFRLQGAYFAIGTWVVAEIVRLLVAQWKTLGGGTGTSLPGAATR
AMFGTDVVQRLLGVRPAQAVDIICYWLALLLAVATIFTVYRLLRSRHGLALASVRDNQEAARAIGVDARRLKAMVYLTSA
ALTGLVGALIYVQKARISPDAAFSVIDWTAYVIFIVVIGGIGTIEGPIVGVLIFFGLQKLLADFGSLYLLALGLIGIVIM
LFAPQGLWGLFASRTGIQLLPVRRLLRGGSIDN

Sequences:

>Translated_353_residues
MHRRSIPWRVETRTKASIVFAVIAALIVVAGVIAPAYLPRSTLQDLFFILTMLVLAQSWNLLAGYAGLVSVGQQAFVGLG
AYAMFAGVVLLKLDPLVSVLLGGAAAALLAIPAGFFAFRLQGAYFAIGTWVVAEIVRLLVAQWKTLGGGTGTSLPGAATR
AMFGTDVVQRLLGVRPAQAVDIICYWLALLLAVATIFTVYRLLRSRHGLALASVRDNQEAARAIGVDARRLKAMVYLTSA
ALTGLVGALIYVQKARISPDAAFSVIDWTAYVIFIVVIGGIGTIEGPIVGVLIFFGLQKLLADFGSLYLLALGLIGIVIM
LFAPQGLWGLFASRTGIQLLPVRRLLRGGSIDN
>Mature_353_residues
MHRRSIPWRVETRTKASIVFAVIAALIVVAGVIAPAYLPRSTLQDLFFILTMLVLAQSWNLLAGYAGLVSVGQQAFVGLG
AYAMFAGVVLLKLDPLVSVLLGGAAAALLAIPAGFFAFRLQGAYFAIGTWVVAEIVRLLVAQWKTLGGGTGTSLPGAATR
AMFGTDVVQRLLGVRPAQAVDIICYWLALLLAVATIFTVYRLLRSRHGLALASVRDNQEAARAIGVDARRLKAMVYLTSA
ALTGLVGALIYVQKARISPDAAFSVIDWTAYVIFIVVIGGIGTIEGPIVGVLIFFGLQKLLADFGSLYLLALGLIGIVIM
LFAPQGLWGLFASRTGIQLLPVRRLLRGGSIDN

Specific function: Part of the binding-protein-dependent transport system for branched-chain amino acids. Probably responsible for the translocation of the substrates across the membrane [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=310, Percent_Identity=30.6451612903226, Blast_Score=94, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 37537; Mature: 37537

Theoretical pI: Translated: 10.83; Mature: 10.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHRRSIPWRVETRTKASIVFAVIAALIVVAGVIAPAYLPRSTLQDLFFILTMLVLAQSWN
CCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCH
LLAGYAGLVSVGQQAFVGLGAYAMFAGVVLLKLDPLVSVLLGGAAAALLAIPAGFFAFRL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QGAYFAIGTWVVAEIVRLLVAQWKTLGGGTGTSLPGAATRAMFGTDVVQRLLGVRPAQAV
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHH
DIICYWLALLLAVATIFTVYRLLRSRHGLALASVRDNQEAARAIGVDARRLKAMVYLTSA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHCCHHHHHHHHHHHHHH
ALTGLVGALIYVQKARISPDAAFSVIDWTAYVIFIVVIGGIGTIEGPIVGVLIFFGLQKL
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
LADFGSLYLLALGLIGIVIMLFAPQGLWGLFASRTGIQLLPVRRLLRGGSIDN
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MHRRSIPWRVETRTKASIVFAVIAALIVVAGVIAPAYLPRSTLQDLFFILTMLVLAQSWN
CCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCH
LLAGYAGLVSVGQQAFVGLGAYAMFAGVVLLKLDPLVSVLLGGAAAALLAIPAGFFAFRL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QGAYFAIGTWVVAEIVRLLVAQWKTLGGGTGTSLPGAATRAMFGTDVVQRLLGVRPAQAV
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHH
DIICYWLALLLAVATIFTVYRLLRSRHGLALASVRDNQEAARAIGVDARRLKAMVYLTSA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHCCHHHHHHHHHHHHHH
ALTGLVGALIYVQKARISPDAAFSVIDWTAYVIFIVVIGGIGTIEGPIVGVLIFFGLQKL
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
LADFGSLYLLALGLIGIVIMLFAPQGLWGLFASRTGIQLLPVRRLLRGGSIDN
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2195019; 8041620; 9278503 [H]