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The map label for this gene is gcvA [H]

Identifier: 146339000

GI number: 146339000

Start: 2072423

End: 2073055

Strand: Reverse

Name: gcvA [H]

Synonym: BRADO1949

Alternate gene names: 146339000

Gene position: 2073055-2072423 (Counterclockwise)

Preceding gene: 146339002

Following gene: 146338998

Centisome position: 27.8

GC content: 65.72

Gene sequence:

>633_bases
ATGCCAGAGTGCGTGACGATGACCTACAGCCTCCCTCCCCTCAACGCCCTGCGTGCCTTCGAAGCGGCGGCGCGGCATCT
CAGCTTCAAGCTGGCCGCGCATGAGCTGCACGTGACGCCGGCCGCGATCGGCCAGCACGTCAAGGCCCTTGAACTACGCC
TCGGGGTGCGGCTCTTCGAGCGTCTGCATAAGCAACTCGTCCTCACCGAAGAGGGGCAAGACTATTTGATCGGGATCTCC
GAGGGCTTTCGCCGCATCGCTGACGCGACCCTGCACCTCAGGCCTCGCTGTGCGGTGCTGCTGCAGCTGGGCGTCCACGA
CAAGTTCGATCTGCGCTGTCTGGACCTGGAGACATTCCGGCGCACACATGCGGAGATCGGCTTGCGCGTGCTGCAGCCGG
CCGGCTTGCACGAACTGATCGAAGGAAAGATCGACGTGCTGATCAGCCGCGGCCTTGGCCATCATCCAGGCTATCGCTCG
GACAGGATCGGTGATGGCAGCGGCCTGGGACACTGGCTGATGGCGTCGGAGGGCACGGCAGACTGCCCCGAACTCGTCAG
CCTCCGCGACTGGCTGCGACGGCGATCAGCGGAGCCCCGGGCCGTGAGCCGCTCCCGCCAGCATGCAGAGTGA

Upstream 100 bases:

>100_bases
GCTCGAGCAGGCAGAGCCTAGCCGGGACCACCCGCGCGCCTCAAAGCAGAGTTTCTTCGGCTCAGCGATAGAAAATCTAT
TGCAGCCGCCTGGCCGAGCC

Downstream 100 bases:

>100_bases
AGCGCCCCGGGAAAACATCCGGGGGCTACCAATGTGCGCGATGAGCCGTAGAGCTTGTGAGCCAATTTGAGTTCAGGGCT
GAGTTTGCGACGAGGGGGCT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 210; Mature: 209

Protein sequence:

>210_residues
MPECVTMTYSLPPLNALRAFEAAARHLSFKLAAHELHVTPAAIGQHVKALELRLGVRLFERLHKQLVLTEEGQDYLIGIS
EGFRRIADATLHLRPRCAVLLQLGVHDKFDLRCLDLETFRRTHAEIGLRVLQPAGLHELIEGKIDVLISRGLGHHPGYRS
DRIGDGSGLGHWLMASEGTADCPELVSLRDWLRRRSAEPRAVSRSRQHAE

Sequences:

>Translated_210_residues
MPECVTMTYSLPPLNALRAFEAAARHLSFKLAAHELHVTPAAIGQHVKALELRLGVRLFERLHKQLVLTEEGQDYLIGIS
EGFRRIADATLHLRPRCAVLLQLGVHDKFDLRCLDLETFRRTHAEIGLRVLQPAGLHELIEGKIDVLISRGLGHHPGYRS
DRIGDGSGLGHWLMASEGTADCPELVSLRDWLRRRSAEPRAVSRSRQHAE
>Mature_209_residues
PECVTMTYSLPPLNALRAFEAAARHLSFKLAAHELHVTPAAIGQHVKALELRLGVRLFERLHKQLVLTEEGQDYLIGISE
GFRRIADATLHLRPRCAVLLQLGVHDKFDLRCLDLETFRRTHAEIGLRVLQPAGLHELIEGKIDVLISRGLGHHPGYRSD
RIGDGSGLGHWLMASEGTADCPELVSLRDWLRRRSAEPRAVSRSRQHAE

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=160, Percent_Identity=40.625, Blast_Score=109, Evalue=1e-25,
Organism=Escherichia coli, GI1786448, Length=74, Percent_Identity=48.6486486486487, Blast_Score=67, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 23561; Mature: 23429

Theoretical pI: Translated: 8.38; Mature: 8.38

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPECVTMTYSLPPLNALRAFEAAARHLSFKLAAHELHVTPAAIGQHVKALELRLGVRLFE
CCCEEEEEECCCCHHHHHHHHHHHHHHHHHEEHHHEECCHHHHHHHHHHHHHHHHHHHHH
RLHKQLVLTEEGQDYLIGISEGFRRIADATLHLRPRCAVLLQLGVHDKFDLRCLDLETFR
HHHHHEEEEECCCCEEEEHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCEEEECHHHHH
RTHAEIGLRVLQPAGLHELIEGKIDVLISRGLGHHPGYRSDRIGDGSGLGHWLMASEGTA
HHHHHHCHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCC
DCPELVSLRDWLRRRSAEPRAVSRSRQHAE
CCHHHHHHHHHHHHCCCCCHHHHHHHCCCC
>Mature Secondary Structure 
PECVTMTYSLPPLNALRAFEAAARHLSFKLAAHELHVTPAAIGQHVKALELRLGVRLFE
CCEEEEEECCCCHHHHHHHHHHHHHHHHHEEHHHEECCHHHHHHHHHHHHHHHHHHHHH
RLHKQLVLTEEGQDYLIGISEGFRRIADATLHLRPRCAVLLQLGVHDKFDLRCLDLETFR
HHHHHEEEEECCCCEEEEHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCEEEECHHHHH
RTHAEIGLRVLQPAGLHELIEGKIDVLISRGLGHHPGYRSDRIGDGSGLGHWLMASEGTA
HHHHHHCHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCC
DCPELVSLRDWLRRRSAEPRAVSRSRQHAE
CCHHHHHHHHHHHHCCCCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]