Click here to switch to the map view.

The map label for this gene is dppA [H]

Identifier: 146338809

GI number: 146338809

Start: 1866415

End: 1868013

Strand: Reverse

Name: dppA [H]

Synonym: BRADO1743

Alternate gene names: 146338809

Gene position: 1868013-1866415 (Counterclockwise)

Preceding gene: 146338810

Following gene: 146338808

Centisome position: 25.05

GC content: 61.16

Gene sequence:

>1599_bases
GTGAACAGATATCACCTGCTCGTTGCCGTCTGCGCCTCGGCGCTGCTGGCAAGTCCGTCGGCCTTTGCGAAGACCCTCGT
GTTCTGCTCGGAAGGCAGCCCGGAGAACTTTTATCCCGGCGTGAACACGACCGGAACGTCTTTCGACGGCAACGAGCCGA
TCTACAACCGGATCGTCGAGTTCGAGCGCGGCGGCACCCAGGTGGTGCCGGGCCTTGCCGAAAGCTGGACGATCTCGCCG
GACGGAACCACCTATACGTTCAATCTGCGCAAGGGCGTGAAGTGGCATTCGACCTCGAAATCGTTCAAGCCGACGCGCGA
CTTCAACGCCGACGACATTCTGTTCTCGATCGAGCGGCAGTGGAAGGAGGACAATCCCTACTTCAAGGTGACCAGCCCAA
ACCATTCCTACTTCGGCGACATGGCGATGCCGAAACTGCTCAAGTCGGTCGAGAAGGTCGACGACTACACGATCAGGATC
ACCCTTAACCAGCCGGAAGCCCCGTTCCTCGCGGATCTCGCGATGCCCTTCGCGGCGATCCAGTCGAAGGAATATGCCGA
CGCGATGTTGAAGGCCGGCACGCCCGAGAAGATCGACCAGGATCCGATCGGCACCGGTCCGTTCTACCTGGTGCAGTACC
AAAAGGACGCGGTCATCCGCTACAAGGCGTTCCCGCAATTCTGGGGCGGCAAGGCCAAGATCGACGACCTCGTCTATGCC
ATCACGCCGGATTCCTCGGTTCGCTGGGCCAAGCTGCAGAAGGGCGAATGCGATGTGATGCCTTATCCGAACCCCGCCGA
CATCGATGCCATCCGCAAGGATCCGAACGTGACGGTGCTCGAGCAGCCCGGCCTCAACGTCGGCTACCTCGCCTTCAACA
CCCAGAAGAAGCCGTTTGACGACGTGCGCGTACGCAAGGCGTTGAGCATGGCGATCGACAAGAAGGCGATCATCGACGCC
GTCTATCTGTCGACCGGCATCGCGGCGAAGAACCCGATCCCGCCGTCGATGTGGTCCTACAACGACTCCGTCAAGGACGA
TCCGTATGATCCCGAGGCGGCCAAGAAACTGCTCGCGGAGGCCGGATTTCCGAATGGCCTCGAGACCGATCTGTGGGCCA
TGCCGGTGCAGCGTCCCTACAATCCGAACGCCAAGCGCATCGCGGAGCTGATGCAGGCCGACCTCGCCAAGATCGGCGTC
AAGGCCGAGATCAAGTCGTTCGAATGGGGCGAATACCGCAAGCGCATGCAGGCCGGCGAGCACCAGATGGGCATGCTCGG
CTGGACCGGCGACAATGGCGACCCGGATAATTTCCTGCACACCCTGCTCGGCTGCGCGTCGGCCCAATCCGGCGGATCCA
ACGTCGCCAAGTTCTGCTACAAGCCGTTCGATGATCTGGTCTTGAAGGCCAAGACGGTCAGCGACCAGACCGAGCGCGCC
AAGCTTTACGGCGAGGCGCAGGTGGTGTTCAAGGAACAGGCGCCATGGTTCACTATCGCGCATGCGGTGCAGTTGAAGCC
GGTCAGCAAGCGCGTGATCGACTTCAAGCTGTCGCCGTTCGGACGGCACAGCTTCTATGGCGTCGACGTCAAGGACTAG

Upstream 100 bases:

>100_bases
GGTGCGTTGACAGCGGCAATACCCGATAGCAAGGTCGGCCAGACGCGGCGATGCAGCCCGTGGCGCATGCGCGTTGCCGT
TCTCACCTGGAGTTCCAACC

Downstream 100 bases:

>100_bases
CGGACGACCCGCGAGGAGTGCCAGGAGCCGCTCTTGGCGTTCCGCCGTCGCGCGGCTTCCCGGCATGGCCACTTGACGAC
CTCCGACTAACTTCTTCTTG

Product: periplasmic dipeptide ABC transporter dipeptide-binding protein

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: Dipeptide-binding protein; DBP [H]

Number of amino acids: Translated: 532; Mature: 532

Protein sequence:

>532_residues
MNRYHLLVAVCASALLASPSAFAKTLVFCSEGSPENFYPGVNTTGTSFDGNEPIYNRIVEFERGGTQVVPGLAESWTISP
DGTTYTFNLRKGVKWHSTSKSFKPTRDFNADDILFSIERQWKEDNPYFKVTSPNHSYFGDMAMPKLLKSVEKVDDYTIRI
TLNQPEAPFLADLAMPFAAIQSKEYADAMLKAGTPEKIDQDPIGTGPFYLVQYQKDAVIRYKAFPQFWGGKAKIDDLVYA
ITPDSSVRWAKLQKGECDVMPYPNPADIDAIRKDPNVTVLEQPGLNVGYLAFNTQKKPFDDVRVRKALSMAIDKKAIIDA
VYLSTGIAAKNPIPPSMWSYNDSVKDDPYDPEAAKKLLAEAGFPNGLETDLWAMPVQRPYNPNAKRIAELMQADLAKIGV
KAEIKSFEWGEYRKRMQAGEHQMGMLGWTGDNGDPDNFLHTLLGCASAQSGGSNVAKFCYKPFDDLVLKAKTVSDQTERA
KLYGEAQVVFKEQAPWFTIAHAVQLKPVSKRVIDFKLSPFGRHSFYGVDVKD

Sequences:

>Translated_532_residues
MNRYHLLVAVCASALLASPSAFAKTLVFCSEGSPENFYPGVNTTGTSFDGNEPIYNRIVEFERGGTQVVPGLAESWTISP
DGTTYTFNLRKGVKWHSTSKSFKPTRDFNADDILFSIERQWKEDNPYFKVTSPNHSYFGDMAMPKLLKSVEKVDDYTIRI
TLNQPEAPFLADLAMPFAAIQSKEYADAMLKAGTPEKIDQDPIGTGPFYLVQYQKDAVIRYKAFPQFWGGKAKIDDLVYA
ITPDSSVRWAKLQKGECDVMPYPNPADIDAIRKDPNVTVLEQPGLNVGYLAFNTQKKPFDDVRVRKALSMAIDKKAIIDA
VYLSTGIAAKNPIPPSMWSYNDSVKDDPYDPEAAKKLLAEAGFPNGLETDLWAMPVQRPYNPNAKRIAELMQADLAKIGV
KAEIKSFEWGEYRKRMQAGEHQMGMLGWTGDNGDPDNFLHTLLGCASAQSGGSNVAKFCYKPFDDLVLKAKTVSDQTERA
KLYGEAQVVFKEQAPWFTIAHAVQLKPVSKRVIDFKLSPFGRHSFYGVDVKD
>Mature_532_residues
MNRYHLLVAVCASALLASPSAFAKTLVFCSEGSPENFYPGVNTTGTSFDGNEPIYNRIVEFERGGTQVVPGLAESWTISP
DGTTYTFNLRKGVKWHSTSKSFKPTRDFNADDILFSIERQWKEDNPYFKVTSPNHSYFGDMAMPKLLKSVEKVDDYTIRI
TLNQPEAPFLADLAMPFAAIQSKEYADAMLKAGTPEKIDQDPIGTGPFYLVQYQKDAVIRYKAFPQFWGGKAKIDDLVYA
ITPDSSVRWAKLQKGECDVMPYPNPADIDAIRKDPNVTVLEQPGLNVGYLAFNTQKKPFDDVRVRKALSMAIDKKAIIDA
VYLSTGIAAKNPIPPSMWSYNDSVKDDPYDPEAAKKLLAEAGFPNGLETDLWAMPVQRPYNPNAKRIAELMQADLAKIGV
KAEIKSFEWGEYRKRMQAGEHQMGMLGWTGDNGDPDNFLHTLLGCASAQSGGSNVAKFCYKPFDDLVLKAKTVSDQTERA
KLYGEAQVVFKEQAPWFTIAHAVQLKPVSKRVIDFKLSPFGRHSFYGVDVKD

Specific function: Dipeptide-binding protein of a transport system that can be subject to osmotic shock. DppA is also required for peptide chemotaxis [H]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789966, Length=527, Percent_Identity=58.6337760910816, Blast_Score=664, Evalue=0.0,
Organism=Escherichia coli, GI1787551, Length=504, Percent_Identity=34.1269841269841, Blast_Score=318, Evalue=4e-88,
Organism=Escherichia coli, GI1787052, Length=549, Percent_Identity=28.7795992714025, Blast_Score=209, Evalue=3e-55,
Organism=Escherichia coli, GI1787762, Length=532, Percent_Identity=25.9398496240602, Blast_Score=154, Evalue=1e-38,
Organism=Escherichia coli, GI1789887, Length=530, Percent_Identity=26.7924528301887, Blast_Score=133, Evalue=3e-32,
Organism=Escherichia coli, GI1789397, Length=473, Percent_Identity=26.0042283298097, Blast_Score=122, Evalue=6e-29,
Organism=Escherichia coli, GI1787495, Length=444, Percent_Identity=26.8018018018018, Blast_Score=118, Evalue=9e-28,
Organism=Escherichia coli, GI87081878, Length=457, Percent_Identity=26.2582056892779, Blast_Score=95, Evalue=1e-20,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2980 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 59353; Mature: 59353

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: PS01040 SBP_BACTERIAL_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRYHLLVAVCASALLASPSAFAKTLVFCSEGSPENFYPGVNTTGTSFDGNEPIYNRIVE
CCCEEEHHHHHHHHHHCCCHHHHEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
FERGGTQVVPGLAESWTISPDGTTYTFNLRKGVKWHSTSKSFKPTRDFNADDILFSIERQ
HCCCCCEECCCCCCCEEECCCCCEEEEECCCCCEECCCCCCCCCCCCCCCCCEEEEEEHH
WKEDNPYFKVTSPNHSYFGDMAMPKLLKSVEKVDDYTIRITLNQPEAPFLADLAMPFAAI
CCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHH
QSKEYADAMLKAGTPEKIDQDPIGTGPFYLVQYQKDAVIRYKAFPQFWGGKAKIDDLVYA
HCHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCEEEEEECCHHHCCCCEEECCEEEE
ITPDSSVRWAKLQKGECDVMPYPNPADIDAIRKDPNVTVLEQPGLNVGYLAFNTQKKPFD
ECCCCCCEEEEECCCCCCCCCCCCCCCCHHHCCCCCEEEEECCCCEEEEEEEECCCCCHH
DVRVRKALSMAIDKKAIIDAVYLSTGIAAKNPIPPSMWSYNDSVKDDPYDPEAAKKLLAE
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
AGFPNGLETDLWAMPVQRPYNPNAKRIAELMQADLAKIGVKAEIKSFEWGEYRKRMQAGE
CCCCCCCCCCCEECCCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHCCC
HQMGMLGWTGDNGDPDNFLHTLLGCASAQSGGSNVAKFCYKPFDDLVLKAKTVSDQTERA
CCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHCCHHHHHEEEHHCCCHHHHH
KLYGEAQVVFKEQAPWFTIAHAVQLKPVSKRVIDFKLSPFGRHSFYGVDVKD
HHCCCEEEEEECCCCEEEEEEEEEECHHHCEEEEEECCCCCCCCEEEEEECC
>Mature Secondary Structure
MNRYHLLVAVCASALLASPSAFAKTLVFCSEGSPENFYPGVNTTGTSFDGNEPIYNRIVE
CCCEEEHHHHHHHHHHCCCHHHHEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
FERGGTQVVPGLAESWTISPDGTTYTFNLRKGVKWHSTSKSFKPTRDFNADDILFSIERQ
HCCCCCEECCCCCCCEEECCCCCEEEEECCCCCEECCCCCCCCCCCCCCCCCEEEEEEHH
WKEDNPYFKVTSPNHSYFGDMAMPKLLKSVEKVDDYTIRITLNQPEAPFLADLAMPFAAI
CCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHH
QSKEYADAMLKAGTPEKIDQDPIGTGPFYLVQYQKDAVIRYKAFPQFWGGKAKIDDLVYA
HCHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCEEEEEECCHHHCCCCEEECCEEEE
ITPDSSVRWAKLQKGECDVMPYPNPADIDAIRKDPNVTVLEQPGLNVGYLAFNTQKKPFD
ECCCCCCEEEEECCCCCCCCCCCCCCCCHHHCCCCCEEEEECCCCEEEEEEEECCCCCHH
DVRVRKALSMAIDKKAIIDAVYLSTGIAAKNPIPPSMWSYNDSVKDDPYDPEAAKKLLAE
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
AGFPNGLETDLWAMPVQRPYNPNAKRIAELMQADLAKIGVKAEIKSFEWGEYRKRMQAGE
CCCCCCCCCCCEECCCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHCCC
HQMGMLGWTGDNGDPDNFLHTLLGCASAQSGGSNVAKFCYKPFDDLVLKAKTVSDQTERA
CCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHCCHHHHHEEEHHCCCHHHHH
KLYGEAQVVFKEQAPWFTIAHAVQLKPVSKRVIDFKLSPFGRHSFYGVDVKD
HHCCCEEEEEECCCCEEEEEEEEEECHHHCEEEEEECCCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1702779; 1956284; 7536291; 8041620; 9278503; 9298646; 9600841; 8563629; 8527431 [H]