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The map label for this gene is gudD [H]

Identifier: 146338635

GI number: 146338635

Start: 1674693

End: 1676048

Strand: Reverse

Name: gudD [H]

Synonym: BRADO1564

Alternate gene names: 146338635

Gene position: 1676048-1674693 (Counterclockwise)

Preceding gene: 146338636

Following gene: 146338634

Centisome position: 22.48

GC content: 67.04

Gene sequence:

>1356_bases
ATGAGTCACGAAGCCACCAGCACAGGAATCTCCGGCGCGCCGGTCGTCACCGGCATGGAGGTCGTTCCGGCCGCCGGGCG
TGACAGCATGCTTCTCAATCTCTCGGGCGCGCATGGGCCGTTCTTCACGCGCAACCTCGTCATCCTGACCGACAATTCCG
GCCATACCGGCGTCGGCGAGGTGCCCGGCGGCGAGAAGATCCGGCAGACGCTGGAGGATGCCAAGGGCTTGATCGTCGGC
CAGAGCATCGGCGCCTGCAACACCATCCTCGCAAAGATGCGCAGCACGTTCGCCGACCGCGATGCCGGCGGCCGCGGCAA
GCAGACGTTCGATCTGCGCGTGATGATCCACGCCGTCACGGCGGTCGAATCCGCTCTGCTCGACCTGCTCGGCCAGCATC
TCGGCCTGCCGGTGGCGGCGCTGCTCGGCGAGGGCCAGCAGCGCAAATCCGTCGAGGCGCTCGGCTATCTGTTCTTCGTC
GGCGACCGCAAGAAGACCGATCTTCCTTATGCCACTGGCGACGGCGAGCGTGAGGGCTGGTACACCTTGCGCCATCAGGA
AGCGATGACGCCGGAGGCCGTGGTGCGCCTCGCCGAGGCTACCCATGACGCCTACGGCTTCGCCGACTTCAAGCTCAAGG
GCGGCGTGCTCAAGGGCGAGGCCGAGATCGAGGCGGTGACCGCGATCGCCAAGCGCTTCCCGCAGGCGCGCGTGACGCTC
GATCCCAACGGCGCCTGGTCGCTGGACGAGGCGATCCGGCTATGCAAGGACATGCACGGCATCCTCGCTTATGCCGAGGA
TCCCTGCGGCGCCGAGGCCGGCTTCTCCGGCCGTGAGATCATGGCCGAATTCCGCCGCGCCACGGGCCTGCCGACCGCGA
CCAACATGATCGCCACCGACTGGCGGCAGCTCAGCCATGCGCTGCGGCTCGGCGCGGTCGACATTCCGCTCGCCGATCCC
CATTTCTGGACCATGCAGGGCTCGGTGCGCGTCGCGCAGACTTGCAAGGACAACGGCCTGACCTGGGGCTCGCACTCCAA
CAACCATTTCGACATTTCGCTCGCCATGTTCACCCATGTCGCCGCCGCCGCGCCCGGCAAGGTCACCGCGATCGACACCC
ATTGGATCTGGCAGGACGGCCAGGCGCTCACCAAACAGCCGCTGCAGATCAAGGGCGGCCGGATCGCAGTGCCCGAGCAA
CCCGGGCTCGGCGTCGAGATTGACCGCGGCGCCATCGAAAAGGCTCATGAACTTTACAAGAGCCACGGGCTCGGCGCACG
AGACGACGCCGTCGCGATGCAGTATCTGATCCCCGGCTGGACCTTCGACGACAAGCGGCCCTGCCTCGTGCGGTGA

Upstream 100 bases:

>100_bases
AGGATGAGGCCACAGCCGGGGCCTCGTGGTTCGAGACGCCCGCCACGTGGCGGGCTCCTCACCATGAGGGGAAGACGAAC
GAGAGACGAAGGAGGAGCAC

Downstream 100 bases:

>100_bases
CGGATGTAATCGGCTAGATTGTGCCAGCACAGTCGCGCAAGCCTGGAGAGTTTTGAAATGAACGCGATCCTCAAGAATTT
CATCGCCGGCGAGTGGGTCG

Product: (D)-glucarate dehydratase 1

Products: NA

Alternate protein names: GDH; GlucD [H]

Number of amino acids: Translated: 451; Mature: 450

Protein sequence:

>451_residues
MSHEATSTGISGAPVVTGMEVVPAAGRDSMLLNLSGAHGPFFTRNLVILTDNSGHTGVGEVPGGEKIRQTLEDAKGLIVG
QSIGACNTILAKMRSTFADRDAGGRGKQTFDLRVMIHAVTAVESALLDLLGQHLGLPVAALLGEGQQRKSVEALGYLFFV
GDRKKTDLPYATGDGEREGWYTLRHQEAMTPEAVVRLAEATHDAYGFADFKLKGGVLKGEAEIEAVTAIAKRFPQARVTL
DPNGAWSLDEAIRLCKDMHGILAYAEDPCGAEAGFSGREIMAEFRRATGLPTATNMIATDWRQLSHALRLGAVDIPLADP
HFWTMQGSVRVAQTCKDNGLTWGSHSNNHFDISLAMFTHVAAAAPGKVTAIDTHWIWQDGQALTKQPLQIKGGRIAVPEQ
PGLGVEIDRGAIEKAHELYKSHGLGARDDAVAMQYLIPGWTFDDKRPCLVR

Sequences:

>Translated_451_residues
MSHEATSTGISGAPVVTGMEVVPAAGRDSMLLNLSGAHGPFFTRNLVILTDNSGHTGVGEVPGGEKIRQTLEDAKGLIVG
QSIGACNTILAKMRSTFADRDAGGRGKQTFDLRVMIHAVTAVESALLDLLGQHLGLPVAALLGEGQQRKSVEALGYLFFV
GDRKKTDLPYATGDGEREGWYTLRHQEAMTPEAVVRLAEATHDAYGFADFKLKGGVLKGEAEIEAVTAIAKRFPQARVTL
DPNGAWSLDEAIRLCKDMHGILAYAEDPCGAEAGFSGREIMAEFRRATGLPTATNMIATDWRQLSHALRLGAVDIPLADP
HFWTMQGSVRVAQTCKDNGLTWGSHSNNHFDISLAMFTHVAAAAPGKVTAIDTHWIWQDGQALTKQPLQIKGGRIAVPEQ
PGLGVEIDRGAIEKAHELYKSHGLGARDDAVAMQYLIPGWTFDDKRPCLVR
>Mature_450_residues
SHEATSTGISGAPVVTGMEVVPAAGRDSMLLNLSGAHGPFFTRNLVILTDNSGHTGVGEVPGGEKIRQTLEDAKGLIVGQ
SIGACNTILAKMRSTFADRDAGGRGKQTFDLRVMIHAVTAVESALLDLLGQHLGLPVAALLGEGQQRKSVEALGYLFFVG
DRKKTDLPYATGDGEREGWYTLRHQEAMTPEAVVRLAEATHDAYGFADFKLKGGVLKGEAEIEAVTAIAKRFPQARVTLD
PNGAWSLDEAIRLCKDMHGILAYAEDPCGAEAGFSGREIMAEFRRATGLPTATNMIATDWRQLSHALRLGAVDIPLADPH
FWTMQGSVRVAQTCKDNGLTWGSHSNNHFDISLAMFTHVAAAAPGKVTAIDTHWIWQDGQALTKQPLQIKGGRIAVPEQP
GLGVEIDRGAIEKAHELYKSHGLGARDDAVAMQYLIPGWTFDDKRPCLVR

Specific function: Catalyzes the dehydration of glucarate to 5-keto-4- deoxy-D-glucarate (5-kdGluc). Also acts on L-idarate [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family. GlucD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789150, Length=439, Percent_Identity=74.2596810933941, Blast_Score=698, Evalue=0.0,
Organism=Escherichia coli, GI1789151, Length=442, Percent_Identity=64.7058823529412, Blast_Score=573, Evalue=1e-165,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017653
- InterPro:   IPR013342
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE [H]

EC number: =4.2.1.40 [H]

Molecular weight: Translated: 48520; Mature: 48389

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHEATSTGISGAPVVTGMEVVPAAGRDSMLLNLSGAHGPFFTRNLVILTDNSGHTGVGE
CCCCCCCCCCCCCCEEECCEEECCCCCCCEEEEECCCCCCEEECEEEEEECCCCCCCCCC
VPGGEKIRQTLEDAKGLIVGQSIGACNTILAKMRSTFADRDAGGRGKQTFDLRVMIHAVT
CCCHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEHHHHHH
AVESALLDLLGQHLGLPVAALLGEGQQRKSVEALGYLFFVGDRKKTDLPYATGDGEREGW
HHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHEEEEEEECCCCCCCCCCCCCCCCCCCE
YTLRHQEAMTPEAVVRLAEATHDAYGFADFKLKGGVLKGEAEIEAVTAIAKRFPQARVTL
EEEECCCCCCHHHHHHHHHHHHHHCCEEEEEEECCEEECCHHHHHHHHHHHHCCCCEEEE
DPNGAWSLDEAIRLCKDMHGILAYAEDPCGAEAGFSGREIMAEFRRATGLPTATNMIATD
CCCCCCCHHHHHHHHHHHHHHEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHH
WRQLSHALRLGAVDIPLADPHFWTMQGSVRVAQTCKDNGLTWGSHSNNHFDISLAMFTHV
HHHHHHHHHCCCEECCCCCCCEEEECCCEEEEEHHCCCCCCCCCCCCCEEEEEHHHHHHH
AAAAPGKVTAIDTHWIWQDGQALTKQPLQIKGGRIAVPEQPGLGVEIDRGAIEKAHELYK
HHCCCCCEEEEEEEEEECCCHHHHCCCEEECCCEEECCCCCCCCEEECCHHHHHHHHHHH
SHGLGARDDAVAMQYLIPGWTFDDKRPCLVR
HCCCCCCCHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SHEATSTGISGAPVVTGMEVVPAAGRDSMLLNLSGAHGPFFTRNLVILTDNSGHTGVGE
CCCCCCCCCCCCCEEECCEEECCCCCCCEEEEECCCCCCEEECEEEEEECCCCCCCCCC
VPGGEKIRQTLEDAKGLIVGQSIGACNTILAKMRSTFADRDAGGRGKQTFDLRVMIHAVT
CCCHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEHHHHHH
AVESALLDLLGQHLGLPVAALLGEGQQRKSVEALGYLFFVGDRKKTDLPYATGDGEREGW
HHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHEEEEEEECCCCCCCCCCCCCCCCCCCE
YTLRHQEAMTPEAVVRLAEATHDAYGFADFKLKGGVLKGEAEIEAVTAIAKRFPQARVTL
EEEECCCCCCHHHHHHHHHHHHHHCCEEEEEEECCEEECCHHHHHHHHHHHHCCCCEEEE
DPNGAWSLDEAIRLCKDMHGILAYAEDPCGAEAGFSGREIMAEFRRATGLPTATNMIATD
CCCCCCCHHHHHHHHHHHHHHEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHH
WRQLSHALRLGAVDIPLADPHFWTMQGSVRVAQTCKDNGLTWGSHSNNHFDISLAMFTHV
HHHHHHHHHCCCEECCCCCCCEEEECCCEEEEEHHCCCCCCCCCCCCCEEEEEHHHHHHH
AAAAPGKVTAIDTHWIWQDGQALTKQPLQIKGGRIAVPEQPGLGVEIDRGAIEKAHELYK
HHCCCCCEEEEEEEEEECCCHHHHCCCEEECCCEEECCCCCCCCEEECCHHHHHHHHHHH
SHGLGARDDAVAMQYLIPGWTFDDKRPCLVR
HCCCCCCCHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]