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The map label for this gene is glnH [H]

Identifier: 146338611

GI number: 146338611

Start: 1646592

End: 1647482

Strand: Reverse

Name: glnH [H]

Synonym: BRADO1539

Alternate gene names: 146338611

Gene position: 1647482-1646592 (Counterclockwise)

Preceding gene: 146338618

Following gene: 146338610

Centisome position: 22.09

GC content: 63.08

Gene sequence:

>891_bases
ATGCAGACAAGGCTATTCACTGGAGCGCGGCGCGCGTGGCTTGCCGCACTCGGCGCCATCATCGCGGCAGGACTTCTCGC
GCCGATCGACGCGCAGGCCGCAACGCCCGCGGGCTGTGCGGCCCTGGCGGAGAAATATCCGGATTGGAAGGGCAAGACTC
TGGTCAACGCCATCAACCCGCACACGCCGGGCTATGAGTCGATCGACCCGAAGGATCCAAGCAAATATGTCGGCTTCGAC
ATCGATCTCGGCGAAGCCATCGGCGACTGCCTCGGCTTCAAGCTCACCTACAAGCCGGTGACCTTCGCCGCGCTGCTGAC
CACGCTGGCGAGCGGCCAGGCCGACATCGTGATCTCCGATATCTACGCCACCAAGGAGCGCGCCAAGGCCGCCGACTTCA
TCACCTATTCCAAGGTGTTCGACGGCGTACTGGTCGCCAAGGGCAACCCGAAGGGCATCAATGGCATCAACATGTCGATG
TGCGGCGCGGCCGCGGCCGAGAACACCGGCTATGTCGAGGTGCCGCTGATCCAGGAGCTCGGGCCCAAGTGCAAAGAGGC
GGGCAAGCCGGAGCCGACACTTCAGCTCTACGACAACAACGCCAACTGCATTCAGGCCATCCTGGCCGGCCGCGCCGACA
CCTATGTCAACGACGTCAACACGGTCGACCAGGCGGTGAAGGCCTATCCGGACAAGCTGGAGAAGGCGATCGCCGTGACC
ATCCCCTATTCGGTCGGCATTGCCGTGCCCAAGGACAAGCCCAAGTTCCGCGATGCCGTGATGGCCGCACTGATCGAGGT
GCAGAAGGCCGGCATCCACCAGGAGCTGTTGAAGAAGTGGGAGCTCGATCCGGCGAACTTCAAGGAGCCTGATATCCTGA
CGGCGGACTGA

Upstream 100 bases:

>100_bases
CGCGGGCGCGCACCTAAGCTTCGTCCGAGCTTGGACCGGGGCGTCGGCACCCATGCACCGCCGGACCAGCGCGACATCAT
CGTTCGAAGGAGCTGGCAAC

Downstream 100 bases:

>100_bases
TAGGCCTGCGGTTCCAAAAGCGAAGCTGCTGCCGCAGCGACGGTGCGCTCCCCTCCCCCTTGCGGGGAGGGGTCGGGGGT
GGGGTCCACAGCGGCAGTCT

Product: ABC transporter substrate-binding protein

Products: ADP; phosphate; L-glutamine [Cytoplasm] [C]

Alternate protein names: GlnBP [H]

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MQTRLFTGARRAWLAALGAIIAAGLLAPIDAQAATPAGCAALAEKYPDWKGKTLVNAINPHTPGYESIDPKDPSKYVGFD
IDLGEAIGDCLGFKLTYKPVTFAALLTTLASGQADIVISDIYATKERAKAADFITYSKVFDGVLVAKGNPKGINGINMSM
CGAAAAENTGYVEVPLIQELGPKCKEAGKPEPTLQLYDNNANCIQAILAGRADTYVNDVNTVDQAVKAYPDKLEKAIAVT
IPYSVGIAVPKDKPKFRDAVMAALIEVQKAGIHQELLKKWELDPANFKEPDILTAD

Sequences:

>Translated_296_residues
MQTRLFTGARRAWLAALGAIIAAGLLAPIDAQAATPAGCAALAEKYPDWKGKTLVNAINPHTPGYESIDPKDPSKYVGFD
IDLGEAIGDCLGFKLTYKPVTFAALLTTLASGQADIVISDIYATKERAKAADFITYSKVFDGVLVAKGNPKGINGINMSM
CGAAAAENTGYVEVPLIQELGPKCKEAGKPEPTLQLYDNNANCIQAILAGRADTYVNDVNTVDQAVKAYPDKLEKAIAVT
IPYSVGIAVPKDKPKFRDAVMAALIEVQKAGIHQELLKKWELDPANFKEPDILTAD
>Mature_296_residues
MQTRLFTGARRAWLAALGAIIAAGLLAPIDAQAATPAGCAALAEKYPDWKGKTLVNAINPHTPGYESIDPKDPSKYVGFD
IDLGEAIGDCLGFKLTYKPVTFAALLTTLASGQADIVISDIYATKERAKAADFITYSKVFDGVLVAKGNPKGINGINMSM
CGAAAAENTGYVEVPLIQELGPKCKEAGKPEPTLQLYDNNANCIQAILAGRADTYVNDVNTVDQAVKAYPDKLEKAIAVT
IPYSVGIAVPKDKPKFRDAVMAALIEVQKAGIHQELLKKWELDPANFKEPDILTAD

Specific function: Involved in a glutamine-transport system glnHPQ [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787031, Length=213, Percent_Identity=29.1079812206573, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: 1440 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 460 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 31553; Mature: 31553

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTRLFTGARRAWLAALGAIIAAGLLAPIDAQAATPAGCAALAEKYPDWKGKTLVNAINP
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCEEEEECCC
HTPGYESIDPKDPSKYVGFDIDLGEAIGDCLGFKLTYKPVTFAALLTTLASGQADIVISD
CCCCCCCCCCCCCCCCEEEECCHHHHHHHHHCCEEEECHHHHHHHHHHHHCCCCCEEEEH
IYATKERAKAADFITYSKVFDGVLVAKGNPKGINGINMSMCGAAAAENTGYVEVPLIQEL
HHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCHHHHCCHHCCCCCEEEECHHHHC
GPKCKEAGKPEPTLQLYDNNANCIQAILAGRADTYVNDVNTVDQAVKAYPDKLEKAIAVT
CHHHHHCCCCCCEEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCHHHHHCEEEEE
IPYSVGIAVPKDKPKFRDAVMAALIEVQKAGIHQELLKKWELDPANFKEPDILTAD
ECCEEEEECCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCEECC
>Mature Secondary Structure
MQTRLFTGARRAWLAALGAIIAAGLLAPIDAQAATPAGCAALAEKYPDWKGKTLVNAINP
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCEEEEECCC
HTPGYESIDPKDPSKYVGFDIDLGEAIGDCLGFKLTYKPVTFAALLTTLASGQADIVISD
CCCCCCCCCCCCCCCCEEEECCHHHHHHHHHCCEEEECHHHHHHHHHHHHCCCCCEEEEH
IYATKERAKAADFITYSKVFDGVLVAKGNPKGINGINMSMCGAAAAENTGYVEVPLIQEL
HHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCHHHHCCHHCCCCCEEEECHHHHC
GPKCKEAGKPEPTLQLYDNNANCIQAILAGRADTYVNDVNTVDQAVKAYPDKLEKAIAVT
CHHHHHCCCCCCEEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCHHHHHCEEEEE
IPYSVGIAVPKDKPKFRDAVMAALIEVQKAGIHQELLKKWELDPANFKEPDILTAD
ECCEEEEECCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamine [Periplasm]; H2O [C]

Specific reaction: ATP + L-glutamine [Periplasm] + H2O = ADP + phosphate + L-glutamine [Cytoplasm] [C]

General reaction: Translocates substrate across the membrane [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]