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The map label for this gene is pckA [H]

Identifier: 146337858

GI number: 146337858

Start: 780390

End: 782003

Strand: Direct

Name: pckA [H]

Synonym: BRADO0741

Alternate gene names: 146337858

Gene position: 780390-782003 (Clockwise)

Preceding gene: 146337857

Following gene: 146337861

Centisome position: 10.47

GC content: 64.56

Gene sequence:

>1614_bases
GTGCAAGAGACGGGTATTCGCAACGGTGCCTTCGGTGCCGACAAGTTCGGCTTAAAAAATCTCAAGCAGTTGCATTGGAA
TCTCGGCGCGCCCCAGCTCTATCAGTATTCGCTGGCGGCGGGCGAGGCCACGCTGTCTGCCGACGGAGCGCTGTGCGCGG
ATACGGGCGAATTCACCGGTCGCAGCCCGAAGGACAAGTTCACCGTGCGCGATGCGTCCACCGAGAAGATGTGGTGGGCG
GGCAATCAGTCGATTACGCCGGAGCAGTTCGAGGCGCTGTATCAGGACTTCATCAAGCACGCCGAAGGCAAGACGCTGTT
CGCGCAGGACCTGTATGGCGGCGCCGATCCGACTTTCCGCATCAAGACCCGCGTCTTCACCGAGCTCGCCTGGCATTCGC
TGTTCATTCGCACGCTTCTCATCCGCCCGGAGACAATCGAGCTCGACACCTTCGTGCCGGAGCTGACGATCATCGACATG
CCGAGCTTCCGCGCCGATCCGATGCGGCACGGCGTGCGCTCGCAGAACGTGGTCGCGATCGATTTCGCCCGCAAGATCGT
GCTGATCGGCGGATCGTATTACGCCGGCGAGATGAAGAAGAGCGTGTTCACCACGCTCAACTACTACCTGCCCGCGCGCG
GTGTAATGCCGATGCACTGCTCGGCCAATGTCGGCCCCAAGGGCGACACCGCGATTTTCTTCGGCCTGTCCGGCACCGGC
AAGACGACGCTGTCGGCCGATCCCAACCGCACGCTGATCGGTGACGACGAGCACGGCTGGGGTCCGTCCGGCGTGTTCAA
CTTCGAGGGCGGCTGCTACGCCAAATGCATCAAGCTCTCGCAGGAGGCCGAGCCGCAGATCTTCGCCGCCTCGAACCGTT
TTGGTGCGGTGCTGGAGAATGTGGTGCTCGACGAGGACAGCCGCGTTCCCGATTTCGACGACGGCTCCAAAACCGAGAAC
ACCCGCTCGGCCTATCCGCTCGACTACATCCCGAACGCCTCGCGCACCGGACGCGCGCCGCATCCGAAGAACGTCGTGAT
GCTCGCCGCCGACGCCTTCGGCGTGCTGCCGCCGATCGCAAAACTGTCGCCGGCGCAGGCGATGTATCACTTCCTGTCCG
GCTACACCGCCAAGGTCGCCGGCACCGAGCGCGGCCTCGGCTCCGAGCCGCAGCCGGAGTTCTCGACCTGCTTCGGCTCG
CCGTTCCTGCCGCTGGATCCCTCCGTCTACGGCAACATGCTGCGGCAGCTGATCGCCGACCACAATGTCGACTGCTGGCT
GGTCAACACCGGCTGGACCGGCGGCAAATACGGCACCGGCTCGCGCATGCCGATCAAGGTGACGCGCGCGCTGTTGACCG
CGGCACTCGACGGCTCGCTTCGCAACGTCGAGTTCCGCACCGACAAATATTTCGGCTTCGCGGTGCCGACGGCGCTGCCG
GGCGTGCCGAGCGAGATCCTCGATCCGGTCAACACCTGGAAGGACAAGGTCGAGTTCGACAAGACCGCCCGCGCGCTCGT
CGGCATGTTCCAGAAGAACTTCGCCAAGTTCGAGGCCCAGGTCGACGCCGATGTCCGCGCCGCCGCGCCCGACGTGAAGC
TCGCGGCGGAGTAG

Upstream 100 bases:

>100_bases
TCGCGACCGCGCTTGGGTAAGGCGATGTAAACGGTAACGGTCACCGCGCGCTGCGCCACGAATTTCGCGCGATCGCTGCG
GGCTCTCAGGAGGGATTTCG

Downstream 100 bases:

>100_bases
GGCTGCAAGCTCAAGGGTCAGCCGCGCTGCGGCTGCCCCTTCGTTTTCGCGCCAAAGCGCGGAGCAGCCGAAGGTCGCGC
CAAACCCACCGCCGTCGTCC

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase [H]

Number of amino acids: Translated: 537; Mature: 537

Protein sequence:

>537_residues
MQETGIRNGAFGADKFGLKNLKQLHWNLGAPQLYQYSLAAGEATLSADGALCADTGEFTGRSPKDKFTVRDASTEKMWWA
GNQSITPEQFEALYQDFIKHAEGKTLFAQDLYGGADPTFRIKTRVFTELAWHSLFIRTLLIRPETIELDTFVPELTIIDM
PSFRADPMRHGVRSQNVVAIDFARKIVLIGGSYYAGEMKKSVFTTLNYYLPARGVMPMHCSANVGPKGDTAIFFGLSGTG
KTTLSADPNRTLIGDDEHGWGPSGVFNFEGGCYAKCIKLSQEAEPQIFAASNRFGAVLENVVLDEDSRVPDFDDGSKTEN
TRSAYPLDYIPNASRTGRAPHPKNVVMLAADAFGVLPPIAKLSPAQAMYHFLSGYTAKVAGTERGLGSEPQPEFSTCFGS
PFLPLDPSVYGNMLRQLIADHNVDCWLVNTGWTGGKYGTGSRMPIKVTRALLTAALDGSLRNVEFRTDKYFGFAVPTALP
GVPSEILDPVNTWKDKVEFDKTARALVGMFQKNFAKFEAQVDADVRAAAPDVKLAAE

Sequences:

>Translated_537_residues
MQETGIRNGAFGADKFGLKNLKQLHWNLGAPQLYQYSLAAGEATLSADGALCADTGEFTGRSPKDKFTVRDASTEKMWWA
GNQSITPEQFEALYQDFIKHAEGKTLFAQDLYGGADPTFRIKTRVFTELAWHSLFIRTLLIRPETIELDTFVPELTIIDM
PSFRADPMRHGVRSQNVVAIDFARKIVLIGGSYYAGEMKKSVFTTLNYYLPARGVMPMHCSANVGPKGDTAIFFGLSGTG
KTTLSADPNRTLIGDDEHGWGPSGVFNFEGGCYAKCIKLSQEAEPQIFAASNRFGAVLENVVLDEDSRVPDFDDGSKTEN
TRSAYPLDYIPNASRTGRAPHPKNVVMLAADAFGVLPPIAKLSPAQAMYHFLSGYTAKVAGTERGLGSEPQPEFSTCFGS
PFLPLDPSVYGNMLRQLIADHNVDCWLVNTGWTGGKYGTGSRMPIKVTRALLTAALDGSLRNVEFRTDKYFGFAVPTALP
GVPSEILDPVNTWKDKVEFDKTARALVGMFQKNFAKFEAQVDADVRAAAPDVKLAAE
>Mature_537_residues
MQETGIRNGAFGADKFGLKNLKQLHWNLGAPQLYQYSLAAGEATLSADGALCADTGEFTGRSPKDKFTVRDASTEKMWWA
GNQSITPEQFEALYQDFIKHAEGKTLFAQDLYGGADPTFRIKTRVFTELAWHSLFIRTLLIRPETIELDTFVPELTIIDM
PSFRADPMRHGVRSQNVVAIDFARKIVLIGGSYYAGEMKKSVFTTLNYYLPARGVMPMHCSANVGPKGDTAIFFGLSGTG
KTTLSADPNRTLIGDDEHGWGPSGVFNFEGGCYAKCIKLSQEAEPQIFAASNRFGAVLENVVLDEDSRVPDFDDGSKTEN
TRSAYPLDYIPNASRTGRAPHPKNVVMLAADAFGVLPPIAKLSPAQAMYHFLSGYTAKVAGTERGLGSEPQPEFSTCFGS
PFLPLDPSVYGNMLRQLIADHNVDCWLVNTGWTGGKYGTGSRMPIKVTRALLTAALDGSLRNVEFRTDKYFGFAVPTALP
GVPSEILDPVNTWKDKVEFDKTARALVGMFQKNFAKFEAQVDADVRAAAPDVKLAAE

Specific function: Rate-limiting gluconeogenic enzyme. [C]

COG id: COG1866

COG function: function code C; Phosphoenolpyruvate carboxykinase (ATP)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [ATP] family [H]

Homologues:

Organism=Escherichia coli, GI1789807, Length=539, Percent_Identity=46.3821892393321, Blast_Score=468, Evalue=1e-133,
Organism=Saccharomyces cerevisiae, GI6322950, Length=515, Percent_Identity=46.0194174757282, Blast_Score=447, Evalue=1e-126,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001272
- InterPro:   IPR013035
- InterPro:   IPR008210
- InterPro:   IPR015994 [H]

Pfam domain/function: PF01293 PEPCK_ATP [H]

EC number: =4.1.1.49 [H]

Molecular weight: Translated: 58743; Mature: 58743

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: PS00532 PEPCK_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQETGIRNGAFGADKFGLKNLKQLHWNLGAPQLYQYSLAAGEATLSADGALCADTGEFTG
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHEEEEHHCCCEEECCCCCEEECCCCCCC
RSPKDKFTVRDASTEKMWWAGNQSITPEQFEALYQDFIKHAEGKTLFAQDLYGGADPTFR
CCCCCCEEEECCCCCCEEECCCCCCCHHHHHHHHHHHHHHCCCCEEEHHHHCCCCCCCEE
IKTRVFTELAWHSLFIRTLLIRPETIELDTFVPELTIIDMPSFRADPMRHGVRSQNVVAI
EHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCEEEEECCCCCCCHHHCCCCCCCEEEE
DFARKIVLIGGSYYAGEMKKSVFTTLNYYLPARGVMPMHCSANVGPKGDTAIFFGLSGTG
EECCEEEEECCCEEHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCEEEEEEECCCC
KTTLSADPNRTLIGDDEHGWGPSGVFNFEGGCYAKCIKLSQEAEPQIFAASNRFGAVLEN
CEEECCCCCCCEECCCCCCCCCCCEEECCCCHHHHHHHHCCCCCCCEEEECCHHHHHHHH
VVLDEDSRVPDFDDGSKTENTRSAYPLDYIPNASRTGRAPHPKNVVMLAADAFGVLPPIA
HHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECHHCCCCCHH
KLSPAQAMYHFLSGYTAKVAGTERGLGSEPQPEFSTCFGSPFLPLDPSVYGNMLRQLIAD
HCCHHHHHHHHHCCCEEEECCCCCCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHC
HNVDCWLVNTGWTGGKYGTGSRMPIKVTRALLTAALDGSLRNVEFRTDKYFGFAVPTALP
CCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCEEEEECCCCCC
GVPSEILDPVNTWKDKVEFDKTARALVGMFQKNFAKFEAQVDADVRAAAPDVKLAAE
CCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCEEEECC
>Mature Secondary Structure
MQETGIRNGAFGADKFGLKNLKQLHWNLGAPQLYQYSLAAGEATLSADGALCADTGEFTG
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHEEEEHHCCCEEECCCCCEEECCCCCCC
RSPKDKFTVRDASTEKMWWAGNQSITPEQFEALYQDFIKHAEGKTLFAQDLYGGADPTFR
CCCCCCEEEECCCCCCEEECCCCCCCHHHHHHHHHHHHHHCCCCEEEHHHHCCCCCCCEE
IKTRVFTELAWHSLFIRTLLIRPETIELDTFVPELTIIDMPSFRADPMRHGVRSQNVVAI
EHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCEEEEECCCCCCCHHHCCCCCCCEEEE
DFARKIVLIGGSYYAGEMKKSVFTTLNYYLPARGVMPMHCSANVGPKGDTAIFFGLSGTG
EECCEEEEECCCEEHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCEEEEEEECCCC
KTTLSADPNRTLIGDDEHGWGPSGVFNFEGGCYAKCIKLSQEAEPQIFAASNRFGAVLEN
CEEECCCCCCCEECCCCCCCCCCCEEECCCCHHHHHHHHCCCCCCCEEEECCHHHHHHHH
VVLDEDSRVPDFDDGSKTENTRSAYPLDYIPNASRTGRAPHPKNVVMLAADAFGVLPPIA
HHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECHHCCCCCHH
KLSPAQAMYHFLSGYTAKVAGTERGLGSEPQPEFSTCFGSPFLPLDPSVYGNMLRQLIAD
HCCHHHHHHHHHCCCEEEECCCCCCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHC
HNVDCWLVNTGWTGGKYGTGSRMPIKVTRALLTAALDGSLRNVEFRTDKYFGFAVPTALP
CCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCEEEEECCCCCC
GVPSEILDPVNTWKDKVEFDKTARALVGMFQKNFAKFEAQVDADVRAAAPDVKLAAE
CCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]