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The map label for this gene is mepA [H]

Identifier: 146337844

GI number: 146337844

Start: 762700

End: 763641

Strand: Reverse

Name: mepA [H]

Synonym: BRADO0725

Alternate gene names: 146337844

Gene position: 763641-762700 (Counterclockwise)

Preceding gene: 146337846

Following gene: 146337843

Centisome position: 10.24

GC content: 69.43

Gene sequence:

>942_bases
ATGCACGTCCGCCTGATTCTGATCGCCGTCATGAGTTCGGCCCTCGTCGCCGCCATGGCACCGGCGCATGCGCAGGACAA
AGGGAGCGTCGAGCCGCAGGCCCTGCCGCCGTTGGCCCATCCGGATGCTCCCGACACCCCGGCCAAGGAGTTGTTCGCTC
GCCGGGTCCTGCCCTCGGCGCAGCCGACCAAGGTCATCGGCACCTATAACCGCGGTTGCATCGCCGGCGCCGAGCGGATG
CCGATCAATGGCGACGACTGGCAGGTGATGCGGCTGTCCAGGAACCGCAATTTCGGCCATCCGCTGCTGATCGCGCTGCT
CAAGCGACTCGCCGCCCGGGCGCACAAGGACGCCGGCTGGCCCGGCATCCTGGTCGGCGACATCGGCCAGCCGCGCGGCG
GACCGGCGCTGAGCGGTCATGCCAGCCACCAGATCGGGCTCGACGCAGACATCTGGCTGACGCCGATGCCCGAGCACCGG
CTGTCACGCAACGAGCGCGAGGACATGTCGGCCGTGATGATGGTCCGGCCCGACCGCCTCGACGTCGACCCGCGCGTGTT
TACGCCGGGACATCTCGCCGTGATCCGCGCCGCGGCCAGGGAGCCGTCGGTGCAACGCATCTTCGTCAACGCCGCGATCA
AGAAGGCGCTGTGCCGCGACGCCAAGGGCGATCGCGGCTGGCTGACGAAGGTGCGGCCGTGGTGGGGTCATGACTACCAC
TTCCACATCCGCATGATGTGTCCGCCCGGCAATCCCGATTGTCACGGCCAGACCGACCAGGAGGGCGGCGACGGCTGCGG
CGACCTCGACTACTGGTTCTCCGACGCGGTGCTGCATCCGCCGCCGCCGAAGGAGCCGCCGAAGCCGCCGCAGCCGATCA
CCATGGCGCAGCTGCCACCGGCCTGCCGCGCGGTACTGCACGCGCCGGATGCCAAGAATTGA

Upstream 100 bases:

>100_bases
ATTTGATCGGTGTTGGGCGGGTTCCACCAATCCGCACGCAGGGTTACTCGCTGCTTAAGATCGTCCTCCTTTGCACGCGT
ACAACGGCTAGACTGGTGTC

Downstream 100 bases:

>100_bases
GGCGCAAGCTCTGACGCGATCGCGTTTCGCGGCGGCGCCGACATGCGCTAGGATGTCCGGGCTCGCGCCGTAAGCGCGGC
ACTCGAACGGGATGACGGTC

Product: penicillin-insensitive murein endopeptidase

Products: Split Peptidoglycan Strands [C]

Alternate protein names: NA

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MHVRLILIAVMSSALVAAMAPAHAQDKGSVEPQALPPLAHPDAPDTPAKELFARRVLPSAQPTKVIGTYNRGCIAGAERM
PINGDDWQVMRLSRNRNFGHPLLIALLKRLAARAHKDAGWPGILVGDIGQPRGGPALSGHASHQIGLDADIWLTPMPEHR
LSRNEREDMSAVMMVRPDRLDVDPRVFTPGHLAVIRAAAREPSVQRIFVNAAIKKALCRDAKGDRGWLTKVRPWWGHDYH
FHIRMMCPPGNPDCHGQTDQEGGDGCGDLDYWFSDAVLHPPPPKEPPKPPQPITMAQLPPACRAVLHAPDAKN

Sequences:

>Translated_313_residues
MHVRLILIAVMSSALVAAMAPAHAQDKGSVEPQALPPLAHPDAPDTPAKELFARRVLPSAQPTKVIGTYNRGCIAGAERM
PINGDDWQVMRLSRNRNFGHPLLIALLKRLAARAHKDAGWPGILVGDIGQPRGGPALSGHASHQIGLDADIWLTPMPEHR
LSRNEREDMSAVMMVRPDRLDVDPRVFTPGHLAVIRAAAREPSVQRIFVNAAIKKALCRDAKGDRGWLTKVRPWWGHDYH
FHIRMMCPPGNPDCHGQTDQEGGDGCGDLDYWFSDAVLHPPPPKEPPKPPQPITMAQLPPACRAVLHAPDAKN
>Mature_313_residues
MHVRLILIAVMSSALVAAMAPAHAQDKGSVEPQALPPLAHPDAPDTPAKELFARRVLPSAQPTKVIGTYNRGCIAGAERM
PINGDDWQVMRLSRNRNFGHPLLIALLKRLAARAHKDAGWPGILVGDIGQPRGGPALSGHASHQIGLDADIWLTPMPEHR
LSRNEREDMSAVMMVRPDRLDVDPRVFTPGHLAVIRAAAREPSVQRIFVNAAIKKALCRDAKGDRGWLTKVRPWWGHDYH
FHIRMMCPPGNPDCHGQTDQEGGDGCGDLDYWFSDAVLHPPPPKEPPKPPQPITMAQLPPACRAVLHAPDAKN

Specific function: Involved in the removal of murein from the sacculus. May also facilitate integration of nascent murein strands into the sacculus by cleaving the peptide bonds between neighboring strands in mature murein [H]

COG id: COG3770

COG function: function code M; Murein endopeptidase

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M74 family [H]

Homologues:

Organism=Escherichia coli, GI1788668, Length=244, Percent_Identity=40.9836065573771, Blast_Score=184, Evalue=1e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009045
- InterPro:   IPR005073 [H]

Pfam domain/function: PF03411 Peptidase_M74 [H]

EC number: 3.4.99.- [C]

Molecular weight: Translated: 34276; Mature: 34276

Theoretical pI: Translated: 8.84; Mature: 8.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHVRLILIAVMSSALVAAMAPAHAQDKGSVEPQALPPLAHPDAPDTPAKELFARRVLPSA
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCC
QPTKVIGTYNRGCIAGAERMPINGDDWQVMRLSRNRNFGHPLLIALLKRLAARAHKDAGW
CCCEEEECCCCCEECCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCC
PGILVGDIGQPRGGPALSGHASHQIGLDADIWLTPMPEHRLSRNEREDMSAVMMVRPDRL
CEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEECCCCHHHHCCCCHHHHHEEEEECCCCC
DVDPRVFTPGHLAVIRAAAREPSVQRIFVNAAIKKALCRDAKGDRGWLTKVRPWWGHDYH
CCCCCEECCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCEE
FHIRMMCPPGNPDCHGQTDQEGGDGCGDLDYWFSDAVLHPPPPKEPPKPPQPITMAQLPP
EEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHCCEECCCCCCCCCCCCCCEECCCCCH
ACRAVLHAPDAKN
HHHHHHCCCCCCC
>Mature Secondary Structure
MHVRLILIAVMSSALVAAMAPAHAQDKGSVEPQALPPLAHPDAPDTPAKELFARRVLPSA
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCC
QPTKVIGTYNRGCIAGAERMPINGDDWQVMRLSRNRNFGHPLLIALLKRLAARAHKDAGW
CCCEEEECCCCCEECCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCC
PGILVGDIGQPRGGPALSGHASHQIGLDADIWLTPMPEHRLSRNEREDMSAVMMVRPDRL
CEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEECCCCHHHHCCCCHHHHHEEEEECCCCC
DVDPRVFTPGHLAVIRAAAREPSVQRIFVNAAIKKALCRDAKGDRGWLTKVRPWWGHDYH
CCCCCEECCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCEE
FHIRMMCPPGNPDCHGQTDQEGGDGCGDLDYWFSDAVLHPPPPKEPPKPPQPITMAQLPP
EEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHCCEECCCCCCCCCCCCCCEECCCCCH
ACRAVLHAPDAKN
HHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: The D-Alanyl-Gamma-Meso-2,6-Diamino- Pimelyl Peptide Bond [C]

Specific reaction: Split The D-Alanyl-Gamma-Meso-2,6-Diamino- Pimelyl Peptide Bond Connecting Neighboring Peptidoglycan Strands. [C]

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Endopeptidases of unknown catalytic mechanism [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]