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The map label for this gene is maiA [H]

Identifier: 146337805

GI number: 146337805

Start: 717822

End: 718454

Strand: Direct

Name: maiA [H]

Synonym: BRADO0684

Alternate gene names: 146337805

Gene position: 717822-718454 (Clockwise)

Preceding gene: 146337803

Following gene: 146337806

Centisome position: 9.63

GC content: 66.19

Gene sequence:

>633_bases
ATGAAGCTGCACGGCTATTTCCGCAGCAGCGCATCCTATCGGGTCAGGATTGCGTTGAACCTCAAGGGGCTGACTGCCGA
GCATCTGCCGCACCACCTCAGGAAAGGCGAGCAGCGCGCGCCCGGCTACCTCGCCCTCAACCCGCAGGGTTTCGTTCCGA
CGCTCGAGGACGACTGCGGCGCTGTTCTCATCCAGTCGCTCGCCATCATCGAATGGCTCGACGAGACTCACCCGGAACCG
CCGCTGCTGCCTAAGGATCCTTTGCGCCGGGCACATATTCGCGCCTTCGCGCAGGTGCTCGCGTGCGATACCCATCCTGT
TCAGAACTTGAAAGTGCTGGCGCGGCTGCGCGAGCTCGGCCTCCCCGAGGACAAAGTCACGGCCTGGGCCGGCTGGGCCA
ATCGCGAGGGACTCGCGGCCTGTGAAGCCCTGGTCAAGCATGAGCCTGGCCCGTTCTGCTTCGGCCCGTCGCCGACGCTC
GCCGATCTCTGTCTCGTGCCCCAACTCGGCAATGCGCGACGCTTCGGCGTCGATGTCGCCGCGTTCCCGCGTCTGCTTCA
GGCCGAAGCCGCAGCCAAGGCGCTGCCGGCGTTTGCCGATGCCGCTCCGGAGCGCCAGCCCGATGCCGAGTAA

Upstream 100 bases:

>100_bases
GCGCGCCTCCCGATCAAATGTCGTTGTTGATTGACAGTATACTGTCAATTAGCCTACCGTCAATTGCAACCAGAGATATC
AACTCAGGAGGAGTGCCACG

Downstream 100 bases:

>100_bases
GCCGCCCGAGATCACGATGGACGCGGTCTATACCAAGCCCGGCTATCTGTTCCGGCGTATGCAGCAGATCGCGGTCTCCA
TCTTCGTGGAGGAATGCCGG

Product: maleylacetoacetate isomerase, MAAI

Products: NA

Alternate protein names: MAAI [H]

Number of amino acids: Translated: 210; Mature: 210

Protein sequence:

>210_residues
MKLHGYFRSSASYRVRIALNLKGLTAEHLPHHLRKGEQRAPGYLALNPQGFVPTLEDDCGAVLIQSLAIIEWLDETHPEP
PLLPKDPLRRAHIRAFAQVLACDTHPVQNLKVLARLRELGLPEDKVTAWAGWANREGLAACEALVKHEPGPFCFGPSPTL
ADLCLVPQLGNARRFGVDVAAFPRLLQAEAAAKALPAFADAAPERQPDAE

Sequences:

>Translated_210_residues
MKLHGYFRSSASYRVRIALNLKGLTAEHLPHHLRKGEQRAPGYLALNPQGFVPTLEDDCGAVLIQSLAIIEWLDETHPEP
PLLPKDPLRRAHIRAFAQVLACDTHPVQNLKVLARLRELGLPEDKVTAWAGWANREGLAACEALVKHEPGPFCFGPSPTL
ADLCLVPQLGNARRFGVDVAAFPRLLQAEAAAKALPAFADAAPERQPDAE
>Mature_210_residues
MKLHGYFRSSASYRVRIALNLKGLTAEHLPHHLRKGEQRAPGYLALNPQGFVPTLEDDCGAVLIQSLAIIEWLDETHPEP
PLLPKDPLRRAHIRAFAQVLACDTHPVQNLKVLARLRELGLPEDKVTAWAGWANREGLAACEALVKHEPGPFCFGPSPTL
ADLCLVPQLGNARRFGVDVAAFPRLLQAEAAAKALPAFADAAPERQPDAE

Specific function: Forms An Equimolar Complex With The RNA Polymerase Holoenzyme (Rnap) But Not With The Core Enzyme. It Is Synthesized Predominantly When Cells Are Exposed To Amino Acid Starvation, At Which Time It Accounts For Over 50% Of The Total Protein Synthesized. It

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GST N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI22202624, Length=209, Percent_Identity=44.4976076555024, Blast_Score=163, Evalue=9e-41,
Organism=Homo sapiens, GI22202622, Length=158, Percent_Identity=42.4050632911392, Blast_Score=119, Evalue=2e-27,
Organism=Homo sapiens, GI194440732, Length=209, Percent_Identity=34.9282296650718, Blast_Score=102, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17551302, Length=211, Percent_Identity=40.2843601895735, Blast_Score=143, Evalue=5e-35,
Organism=Caenorhabditis elegans, GI17510461, Length=210, Percent_Identity=35.2380952380952, Blast_Score=117, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI17556142, Length=199, Percent_Identity=36.1809045226131, Blast_Score=108, Evalue=1e-24,
Organism=Drosophila melanogaster, GI21355859, Length=210, Percent_Identity=41.4285714285714, Blast_Score=149, Evalue=1e-36,
Organism=Drosophila melanogaster, GI24645375, Length=210, Percent_Identity=41.4285714285714, Blast_Score=149, Evalue=1e-36,
Organism=Drosophila melanogaster, GI45553325, Length=210, Percent_Identity=41.4285714285714, Blast_Score=149, Evalue=1e-36,
Organism=Drosophila melanogaster, GI21355857, Length=210, Percent_Identity=37.1428571428571, Blast_Score=139, Evalue=1e-33,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1982 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010987
- InterPro:   IPR004045
- InterPro:   IPR017933
- InterPro:   IPR004046
- InterPro:   IPR005955
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00043 GST_C; PF02798 GST_N [H]

EC number: =5.2.1.2 [H]

Molecular weight: Translated: 22962; Mature: 22962

Theoretical pI: Translated: 7.22; Mature: 7.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLHGYFRSSASYRVRIALNLKGLTAEHLPHHLRKGEQRAPGYLALNPQGFVPTLEDDCG
CCCCCCCCCCCCEEEEEEEEECCCCHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCHHH
AVLIQSLAIIEWLDETHPEPPLLPKDPLRRAHIRAFAQVLACDTHPVQNLKVLARLRELG
HHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCC
LPEDKVTAWAGWANREGLAACEALVKHEPGPFCFGPSPTLADLCLVPQLGNARRFGVDVA
CCHHHHEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCCCHHHCCCHH
AFPRLLQAEAAAKALPAFADAAPERQPDAE
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MKLHGYFRSSASYRVRIALNLKGLTAEHLPHHLRKGEQRAPGYLALNPQGFVPTLEDDCG
CCCCCCCCCCCCEEEEEEEEECCCCHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCHHH
AVLIQSLAIIEWLDETHPEPPLLPKDPLRRAHIRAFAQVLACDTHPVQNLKVLARLRELG
HHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCC
LPEDKVTAWAGWANREGLAACEALVKHEPGPFCFGPSPTLADLCLVPQLGNARRFGVDVA
CCHHHHEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCCCHHHCCCHH
AFPRLLQAEAAAKALPAFADAAPERQPDAE
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]