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The map label for this gene is yiaO [H]

Identifier: 146337798

GI number: 146337798

Start: 710037

End: 711017

Strand: Direct

Name: yiaO [H]

Synonym: BRADO0677

Alternate gene names: 146337798

Gene position: 710037-711017 (Clockwise)

Preceding gene: 146337797

Following gene: 146337799

Centisome position: 9.52

GC content: 64.42

Gene sequence:

>981_bases
ATGCGTAGCTCGCTTCGAGAGAGTTTGTTTGCAGGACTGATGGCGGCGGTGCTCGTCGGCGTCCATCCGGCGGCGGCATC
CCCGCGAATGGTCTCGCTGGTGTTCAACTCGCAGCCGCAAAACCCGCAGCAGATCGGCTCCGATGAGTTTCGCACCAAGC
TGCAGGCGCTTGCCGGCAAGACGCTGATCATCGACGAACGCGCCGGCAATTCGTTCGGCAGCGAAGCGGCCGTCCTGGCG
GCGACCCGCACGGGCGCGGTCGACATCGCGGTGGTGTCCGGCGGCATCGCCAGCGCCGTCGTTCCCGAACTCGGTGTCTT
CGACATCCCCTTCCTGTTCCGCGATACCGCGCACGCCAAGGCCGTCGCCACGGGACCGGTCGCCACCGCGATCGGCGCCA
AGTTTACGGACAAGGGGCTGGTGCTGCTCGCCCTGGGCAAGCAGGGCTTCCGCAATCTCACCAATTCGAAGCGGCCGGTA
CGCACGGTTGATGATGTCAAGGGTCTGAAGATCCGCGTCATCCCCAATCCCGTCTATCAGATGACTTTCAAGGCCCTGGG
CGCCGATGTCCTGCCGATGGACTTTCCCCTCGTGTACGCGGCGCTCAAGGACGGGCGCCTCGATGGTCAGGAGAACCCCG
TCGCCACGATCGCCGCCAACCGGTTCGAGGAGGTACAAAAGTATCTCACGCTGACCGGCCATTTCTTCGCGCCGATCGCC
TTCGTCGCCAATCGTGCGACGTTCGAGCAGCTCGATGCGGGTGAGCGCGAAGCGCTGGTCGCGGCTGCCAAGGCCGCGGC
GGAAGTCACGTGGCAGGCGCAGCTCGATGCCCAGAAGCTCGACGATCTGCGCAAGGGCGGCATGGACGTCATCGAGACCT
TCGATCGCAAGACCTTCGTCGATGCGGTCAAGCCGCTCGATCCCGAATTCGAGAAGCGCTTTGGCAAGGAGCTGCTCGCT
ACCATCAGGTCCACTCCGTGA

Upstream 100 bases:

>100_bases
GATTGGCAAGAACCGGCGAGCTTGTTAGGACGAGGAGCGATCTTAACATCTTAACCATATTTCGCTGCTACGAAAGTTAT
GAGAAAAGCTTGAGGATCTC

Downstream 100 bases:

>100_bases
AACAGACCGGCGATTCCATGAAACTCTCTTCGTTGATGTCCTGGATCGGGGTCCGGCCGCGGATCTTCGGCGGCTTCGCG
CTGATCCTGAGCTTTCTGGT

Product: putative TRAP-dicarboxylate transporter periplasmic protein DctP

Products: NA

Alternate protein names: 2,3-DKG-binding protein; Extracytoplasmic solute receptor protein yiaO [H]

Number of amino acids: Translated: 326; Mature: 326

Protein sequence:

>326_residues
MRSSLRESLFAGLMAAVLVGVHPAAASPRMVSLVFNSQPQNPQQIGSDEFRTKLQALAGKTLIIDERAGNSFGSEAAVLA
ATRTGAVDIAVVSGGIASAVVPELGVFDIPFLFRDTAHAKAVATGPVATAIGAKFTDKGLVLLALGKQGFRNLTNSKRPV
RTVDDVKGLKIRVIPNPVYQMTFKALGADVLPMDFPLVYAALKDGRLDGQENPVATIAANRFEEVQKYLTLTGHFFAPIA
FVANRATFEQLDAGEREALVAAAKAAAEVTWQAQLDAQKLDDLRKGGMDVIETFDRKTFVDAVKPLDPEFEKRFGKELLA
TIRSTP

Sequences:

>Translated_326_residues
MRSSLRESLFAGLMAAVLVGVHPAAASPRMVSLVFNSQPQNPQQIGSDEFRTKLQALAGKTLIIDERAGNSFGSEAAVLA
ATRTGAVDIAVVSGGIASAVVPELGVFDIPFLFRDTAHAKAVATGPVATAIGAKFTDKGLVLLALGKQGFRNLTNSKRPV
RTVDDVKGLKIRVIPNPVYQMTFKALGADVLPMDFPLVYAALKDGRLDGQENPVATIAANRFEEVQKYLTLTGHFFAPIA
FVANRATFEQLDAGEREALVAAAKAAAEVTWQAQLDAQKLDDLRKGGMDVIETFDRKTFVDAVKPLDPEFEKRFGKELLA
TIRSTP
>Mature_326_residues
MRSSLRESLFAGLMAAVLVGVHPAAASPRMVSLVFNSQPQNPQQIGSDEFRTKLQALAGKTLIIDERAGNSFGSEAAVLA
ATRTGAVDIAVVSGGIASAVVPELGVFDIPFLFRDTAHAKAVATGPVATAIGAKFTDKGLVLLALGKQGFRNLTNSKRPV
RTVDDVKGLKIRVIPNPVYQMTFKALGADVLPMDFPLVYAALKDGRLDGQENPVATIAANRFEEVQKYLTLTGHFFAPIA
FVANRATFEQLDAGEREALVAAAKAAAEVTWQAQLDAQKLDDLRKGGMDVIETFDRKTFVDAVKPLDPEFEKRFGKELLA
TIRSTP

Specific function: Part of the tripartite ATP-independent periplasmic (TRAP) transport system yiaMNO involved in the uptake of 2,3- diketo-L-gulonate. This protein specifically binds 2,3-diketo-L- gulonate. Is not able to bind either L-ascorbate or dehydroascorbate [H]

COG id: COG1638

COG function: function code G; TRAP-type C4-dicarboxylate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 7 family [H]

Homologues:

Organism=Escherichia coli, GI1790004, Length=303, Percent_Identity=33.6633663366337, Blast_Score=175, Evalue=5e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018389
- InterPro:   IPR004682 [H]

Pfam domain/function: PF03480 SBP_bac_7 [H]

EC number: NA

Molecular weight: Translated: 34952; Mature: 34952

Theoretical pI: Translated: 9.36; Mature: 9.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSSLRESLFAGLMAAVLVGVHPAAASPRMVSLVFNSQPQNPQQIGSDEFRTKLQALAGK
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCHHHCCCHHHHHHHHHHCCC
TLIIDERAGNSFGSEAAVLAATRTGAVDIAVVSGGIASAVVPELGVFDIPFLFRDTAHAK
EEEEECCCCCCCCCCCEEEEEECCCCEEEEEECCCHHHHHHCCCCCCCCCHHHHCCCCHH
AVATGPVATAIGAKFTDKGLVLLALGKQGFRNLTNSKRPVRTVDDVKGLKIRVIPNPVYQ
EEECCCHHHHHCCCCCCCCEEEEEECCHHHHHHCCCCCCCCCHHCCCCCEEEECCCHHHH
MTFKALGADVLPMDFPLVYAALKDGRLDGQENPVATIAANRFEEVQKYLTLTGHFFAPIA
HHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHH
FVANRATFEQLDAGEREALVAAAKAAAEVTWQAQLDAQKLDDLRKGGMDVIETFDRKTFV
HHHCCHHHHHHCCCCHHHHHHHHHHHHHEEEHHHCCHHHHHHHHHCCHHHHHHHHHHHHH
DAVKPLDPEFEKRFGKELLATIRSTP
HHHCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRSSLRESLFAGLMAAVLVGVHPAAASPRMVSLVFNSQPQNPQQIGSDEFRTKLQALAGK
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCHHHCCCHHHHHHHHHHCCC
TLIIDERAGNSFGSEAAVLAATRTGAVDIAVVSGGIASAVVPELGVFDIPFLFRDTAHAK
EEEEECCCCCCCCCCCEEEEEECCCCEEEEEECCCHHHHHHCCCCCCCCCHHHHCCCCHH
AVATGPVATAIGAKFTDKGLVLLALGKQGFRNLTNSKRPVRTVDDVKGLKIRVIPNPVYQ
EEECCCHHHHHCCCCCCCCEEEEEECCHHHHHHCCCCCCCCCHHCCCCCEEEECCCHHHH
MTFKALGADVLPMDFPLVYAALKDGRLDGQENPVATIAANRFEEVQKYLTLTGHFFAPIA
HHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHH
FVANRATFEQLDAGEREALVAAAKAAAEVTWQAQLDAQKLDDLRKGGMDVIETFDRKTFV
HHHCCHHHHHHCCCCHHHHHHHHHHHHHEEEHHHCCHHHHHHHHHCCHHHHHHHHHHHHH
DAVKPLDPEFEKRFGKELLATIRSTP
HHHCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503 [H]