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The map label for this gene is citZ [H]

Identifier: 146337742

GI number: 146337742

Start: 638837

End: 640078

Strand: Direct

Name: citZ [H]

Synonym: BRADO0614

Alternate gene names: 146337742

Gene position: 638837-640078 (Clockwise)

Preceding gene: 146337740

Following gene: 146337744

Centisome position: 8.57

GC content: 68.12

Gene sequence:

>1242_bases
TTGATTAAGCTGATCAATATGAAAAAAGACAGCGGCCTTTACCTATCGGCCCGTGAGGCCTCCGCCGAACTCGCGATCAC
GCCCGCGACGCTGTATGCCTATGTGAGCCGCGGCTTGGTCCGGTCCGAGCCGTCGCCAGATTCGCGCAGCAACCGCTATC
GCGCCGAGGACATCCGGGCGTTGAAGGAGCGGCGTGCGCCCGCGCCGGAGCCGCGAGCCTTCCGCAGTTTTGATGCCGAT
CTGCCGGTGTTCGACTCTGCGGTGTCGACGATCACCGAAGCCGGTCCGATCTACCGCGGCGTGAACTGCGTCGAGCTGGC
GGAGCGGGACTCGCTGGAGCATGCGGCGACCCTGCTGTGGGACGTCACGGCACTCGATCCGTTCGCGCCCGAGAATATGC
CGCACATCTCGGACGACATGCGCCTCATTGCCGAGGCGGCACGGCGCGCCGCGCCGATTGACCGCGCCATCGCAGTGCTG
GCGCTGGCGACAAACGCGGATCCCGCCGCGTTCAACCGTGCTCCCGACGGACGCGCCCTGATCGGCGCGCGCATCGTTCG
GCTCGTGGTCGCGACCATGCTGAATGCGCCGCCTTCTGCGGAGGCGCTCCATGTCCAAATCGCCAAAACCTGGGCGCCAG
ACAACAAGCATGCGCCCGACCTGATTCGCCGGACCCTCGTGCTGCTCGCCGACCACGAGCTGAACGCTTCGACCTTCACC
GTCCGCTGCGCCGCATCGACGGGCCTCAGCCTCTATGACGCCGTGATCGCCGGCCTCGCCGCCCTGAAGGGGCCGCAGCA
TGGCGGCGCCGGCGTGCTGGCATCGCAACTGGTGAAGACGCTGATCGATCGCGACGTCGCGCCGCTGATCCGTGAACGCG
TTGCGCTGGGCGAGCGCTTCGCCGGCTTCGGACACGGCGTCTACAAGCGCGGCGATCCGCGCGCCATCTCGTTGCTGGAG
GCGCTGACGCGCGCCGGCGCGCCACGCAAGTTCACCCGCGAGGTGCCCGAGCGGATCGCGGAGGCGACCGGCGAGTTCGT
CAACATCGACTATGCGCTAGCCGTGCTGGTGCACAGCCTGCGGCTGCCGGCCGGCAGCGAGCTGGCGCTATTCGCGATGG
CGCGCTGCGTCGGCTGGATCGCGCATGCCTGCGAGCAATTGCAGTTCGGCCGCCTAATCCGGCCACGCGCACGCTACACC
GGACCGATCCCGGGACGCGCCGCCAGCCTTGAAACGCTGTAG

Upstream 100 bases:

>100_bases
GAGGCCGATCGGGGACTTCGGAAGCTGAATGGTCATGGCGCGATCTCCTGCGTGAATGGACACGCCGGTAAGATTCGTCC
TGTCGACAAATTGATCAATC

Downstream 100 bases:

>100_bases
CGGCGCGGCCCCGCCTATTCGGCAGAGCCAGTCGCCAGCTGCGTCTCGCGCCGCTTCCGCACCATCCGCAGCACCAGCCA
GACCACGATCGTGCCGGCGA

Product: putative citrate synthase

Products: NA

Alternate protein names: Citrate synthase II [H]

Number of amino acids: Translated: 413; Mature: 413

Protein sequence:

>413_residues
MIKLINMKKDSGLYLSAREASAELAITPATLYAYVSRGLVRSEPSPDSRSNRYRAEDIRALKERRAPAPEPRAFRSFDAD
LPVFDSAVSTITEAGPIYRGVNCVELAERDSLEHAATLLWDVTALDPFAPENMPHISDDMRLIAEAARRAAPIDRAIAVL
ALATNADPAAFNRAPDGRALIGARIVRLVVATMLNAPPSAEALHVQIAKTWAPDNKHAPDLIRRTLVLLADHELNASTFT
VRCAASTGLSLYDAVIAGLAALKGPQHGGAGVLASQLVKTLIDRDVAPLIRERVALGERFAGFGHGVYKRGDPRAISLLE
ALTRAGAPRKFTREVPERIAEATGEFVNIDYALAVLVHSLRLPAGSELALFAMARCVGWIAHACEQLQFGRLIRPRARYT
GPIPGRAASLETL

Sequences:

>Translated_413_residues
MIKLINMKKDSGLYLSAREASAELAITPATLYAYVSRGLVRSEPSPDSRSNRYRAEDIRALKERRAPAPEPRAFRSFDAD
LPVFDSAVSTITEAGPIYRGVNCVELAERDSLEHAATLLWDVTALDPFAPENMPHISDDMRLIAEAARRAAPIDRAIAVL
ALATNADPAAFNRAPDGRALIGARIVRLVVATMLNAPPSAEALHVQIAKTWAPDNKHAPDLIRRTLVLLADHELNASTFT
VRCAASTGLSLYDAVIAGLAALKGPQHGGAGVLASQLVKTLIDRDVAPLIRERVALGERFAGFGHGVYKRGDPRAISLLE
ALTRAGAPRKFTREVPERIAEATGEFVNIDYALAVLVHSLRLPAGSELALFAMARCVGWIAHACEQLQFGRLIRPRARYT
GPIPGRAASLETL
>Mature_413_residues
MIKLINMKKDSGLYLSAREASAELAITPATLYAYVSRGLVRSEPSPDSRSNRYRAEDIRALKERRAPAPEPRAFRSFDAD
LPVFDSAVSTITEAGPIYRGVNCVELAERDSLEHAATLLWDVTALDPFAPENMPHISDDMRLIAEAARRAAPIDRAIAVL
ALATNADPAAFNRAPDGRALIGARIVRLVVATMLNAPPSAEALHVQIAKTWAPDNKHAPDLIRRTLVLLADHELNASTFT
VRCAASTGLSLYDAVIAGLAALKGPQHGGAGVLASQLVKTLIDRDVAPLIRERVALGERFAGFGHGVYKRGDPRAISLLE
ALTRAGAPRKFTREVPERIAEATGEFVNIDYALAVLVHSLRLPAGSELALFAMARCVGWIAHACEQLQFGRLIRPRARYT
GPIPGRAASLETL

Specific function: Might regulate the synthesis and function of enzymes involved in later enzymatic steps of Krebs cycle. Loss in activity results in sporulation defect [H]

COG id: COG0372

COG function: function code C; Citrate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the citrate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786527, Length=186, Percent_Identity=38.7096774193548, Blast_Score=119, Evalue=4e-28,
Organism=Escherichia coli, GI1786939, Length=197, Percent_Identity=36.5482233502538, Blast_Score=105, Evalue=6e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011278
- InterPro:   IPR016142
- InterPro:   IPR016143
- InterPro:   IPR002020
- InterPro:   IPR016141
- InterPro:   IPR019810 [H]

Pfam domain/function: PF00285 Citrate_synt [H]

EC number: =2.3.3.1 [H]

Molecular weight: Translated: 44618; Mature: 44618

Theoretical pI: Translated: 9.11; Mature: 9.11

Prosite motif: PS00480 CITRATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKLINMKKDSGLYLSAREASAELAITPATLYAYVSRGLVRSEPSPDSRSNRYRAEDIRA
CEEEEEECCCCCCEEEECCCCCEEEECHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHH
LKERRAPAPEPRAFRSFDADLPVFDSAVSTITEAGPIYRGVNCVELAERDSLEHAATLLW
HHHHCCCCCCCHHHHCCCCCCCHHHHHHHHHHHCCCHHCCCCHHHHHHHCCHHHHHHHHH
DVTALDPFAPENMPHISDDMRLIAEAARRAAPIDRAIAVLALATNADPAAFNRAPDGRAL
HHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHEECCCCHHHCCCCCCCHHH
IGARIVRLVVATMLNAPPSAEALHVQIAKTWAPDNKHAPDLIRRTLVLLADHELNASTFT
HHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCEEE
VRCAASTGLSLYDAVIAGLAALKGPQHGGAGVLASQLVKTLIDRDVAPLIRERVALGERF
EEEECCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
AGFGHGVYKRGDPRAISLLEALTRAGAPRKFTREVPERIAEATGEFVNIDYALAVLVHSL
HHCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHH
RLPAGSELALFAMARCVGWIAHACEQLQFGRLIRPRARYTGPIPGRAASLETL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MIKLINMKKDSGLYLSAREASAELAITPATLYAYVSRGLVRSEPSPDSRSNRYRAEDIRA
CEEEEEECCCCCCEEEECCCCCEEEECHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHH
LKERRAPAPEPRAFRSFDADLPVFDSAVSTITEAGPIYRGVNCVELAERDSLEHAATLLW
HHHHCCCCCCCHHHHCCCCCCCHHHHHHHHHHHCCCHHCCCCHHHHHHHCCHHHHHHHHH
DVTALDPFAPENMPHISDDMRLIAEAARRAAPIDRAIAVLALATNADPAAFNRAPDGRAL
HHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHEECCCCHHHCCCCCCCHHH
IGARIVRLVVATMLNAPPSAEALHVQIAKTWAPDNKHAPDLIRRTLVLLADHELNASTFT
HHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCEEE
VRCAASTGLSLYDAVIAGLAALKGPQHGGAGVLASQLVKTLIDRDVAPLIRERVALGERF
EEEECCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
AGFGHGVYKRGDPRAISLLEALTRAGAPRKFTREVPERIAEATGEFVNIDYALAVLVHSL
HHCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHH
RLPAGSELALFAMARCVGWIAHACEQLQFGRLIRPRARYTGPIPGRAASLETL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8045898; 9387221; 9384377 [H]