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The map label for this gene is citA [H]

Identifier: 146337741

GI number: 146337741

Start: 637684

End: 638799

Strand: Reverse

Name: citA [H]

Synonym: BRADO0613

Alternate gene names: 146337741

Gene position: 638799-637684 (Counterclockwise)

Preceding gene: 146337743

Following gene: 146337739

Centisome position: 8.57

GC content: 69.98

Gene sequence:

>1116_bases
GTGTCCATTCACGCAGGAGATCGCGCCATGACCATTCAGCTTCCGAAGTCCCCGATCGGCCTCGACGGCATTCCCGCCGC
CGAGACCGTGCTCAGCCATGTCGACGGCCAGCAGGGCGAGCTGATCATTGCAGGCCACCGCGTAACCGACCTCGCCGCCG
GAACCGGCTTCGAGGGGGTGACCGCACGGCTCTGGAGCGCGGCGACCAGACGCAGCCTCAGCGAGGCCGACGTCCGCGTC
ACCCTCGGCGTGGCACGCCAGCGCGCCTTCGCGCGGCTGCCCGAGCTGCTCGCTGCGACGACGGGCCTGTCGATCATCGA
CGGCTTCCGCGCCGCCATCGCCGCGCTCCGGCCCGAGCCGGGCCTCGACGACGAAGCCACCATCGTCGGCGCCTGTCCCG
TCATCGCCGGCGCCCTGGTCCAGCGCGCCCGCGGCGCGCAGCCGATCGCGCCGAATCCTGACGTGAGCCATGCCTCCGAC
ACCCTGCGCATGGTCCGCGGCGATACCCCTGCCGCTGGCGAGGTGGCGGCGCTCGAAACCTATCTCGTGACCGTCAGCGA
CCACGGCATGAACGCCTCGACCTTTGCCGCCCGCGTCGTCGCCTCCACCCAAGCCGACCTGTTCGCCTCGATCACCGCCG
GCTATTGCGCGCTGACGGGCCCGCTGCATGGCGGGGCGCCGGAGCCGGTGTTGGAGATGCTGGATGCGATCGGGACCCGG
GAGAACATCAAGCCGTGGGTGGATGCGGCGTTATCGCGCGGGGAGCGGCTGATGGGGTTTGGGCATCGGATCTATCAGGT
GCGGGATCCCCGGGCCGATGTGCTCAAGGGCGCGATCAAGCGGCTGGCGGGGAGCGGGGCGGATCTGCCGTTCGCGAGCG
AGGTGGAGGCTTATATCCGGCAGGCGCTGCGGCAGAAGAATCCGGAGCGGGTGTTGGACACCAATGTCGAGTTCTTCACC
GCCATCCTGCTCGATGCGCTGAAGATTCCGCGCCAGGCGTTCACGCCGATCTTCGCGGTGGCCCGCGCCGCGGGCTGGAC
CGCTCATGCCCGCGAGCAGCAGCGGACGGGTCGGCTGATCCGGCCAAGCTCGGCCTATATCGGGCCGAAGCCCTAA

Upstream 100 bases:

>100_bases
GGAGGCCTCACGGGCCGATAGGTAAAGGCCGCTGTCTTTTTTCATATTGATCAGCTTAATCAAGATTGATCAATTTGTCG
ACAGGACGAATCTTACCGGC

Downstream 100 bases:

>100_bases
ACACCCAGAATGCGACGCGCGGCAGCCTCGCTGCCGCGTGTCAGCCCTTCACCACCGTCTTCAGGTAGTTGTAGGCCTTG
ATGATCTCGATCAGGCGATC

Product: citrate synthase 2

Products: NA

Alternate protein names: Citrate synthase I [H]

Number of amino acids: Translated: 371; Mature: 370

Protein sequence:

>371_residues
MSIHAGDRAMTIQLPKSPIGLDGIPAAETVLSHVDGQQGELIIAGHRVTDLAAGTGFEGVTARLWSAATRRSLSEADVRV
TLGVARQRAFARLPELLAATTGLSIIDGFRAAIAALRPEPGLDDEATIVGACPVIAGALVQRARGAQPIAPNPDVSHASD
TLRMVRGDTPAAGEVAALETYLVTVSDHGMNASTFAARVVASTQADLFASITAGYCALTGPLHGGAPEPVLEMLDAIGTR
ENIKPWVDAALSRGERLMGFGHRIYQVRDPRADVLKGAIKRLAGSGADLPFASEVEAYIRQALRQKNPERVLDTNVEFFT
AILLDALKIPRQAFTPIFAVARAAGWTAHAREQQRTGRLIRPSSAYIGPKP

Sequences:

>Translated_371_residues
MSIHAGDRAMTIQLPKSPIGLDGIPAAETVLSHVDGQQGELIIAGHRVTDLAAGTGFEGVTARLWSAATRRSLSEADVRV
TLGVARQRAFARLPELLAATTGLSIIDGFRAAIAALRPEPGLDDEATIVGACPVIAGALVQRARGAQPIAPNPDVSHASD
TLRMVRGDTPAAGEVAALETYLVTVSDHGMNASTFAARVVASTQADLFASITAGYCALTGPLHGGAPEPVLEMLDAIGTR
ENIKPWVDAALSRGERLMGFGHRIYQVRDPRADVLKGAIKRLAGSGADLPFASEVEAYIRQALRQKNPERVLDTNVEFFT
AILLDALKIPRQAFTPIFAVARAAGWTAHAREQQRTGRLIRPSSAYIGPKP
>Mature_370_residues
SIHAGDRAMTIQLPKSPIGLDGIPAAETVLSHVDGQQGELIIAGHRVTDLAAGTGFEGVTARLWSAATRRSLSEADVRVT
LGVARQRAFARLPELLAATTGLSIIDGFRAAIAALRPEPGLDDEATIVGACPVIAGALVQRARGAQPIAPNPDVSHASDT
LRMVRGDTPAAGEVAALETYLVTVSDHGMNASTFAARVVASTQADLFASITAGYCALTGPLHGGAPEPVLEMLDAIGTRE
NIKPWVDAALSRGERLMGFGHRIYQVRDPRADVLKGAIKRLAGSGADLPFASEVEAYIRQALRQKNPERVLDTNVEFFTA
ILLDALKIPRQAFTPIFAVARAAGWTAHAREQQRTGRLIRPSSAYIGPKP

Specific function: Might regulate the synthesis and function of enzymes involved in later enzymatic steps of Krebs cycle. Loss in activity results in sporulation defect [H]

COG id: COG0372

COG function: function code C; Citrate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the citrate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786527, Length=377, Percent_Identity=28.6472148541114, Blast_Score=151, Evalue=7e-38,
Organism=Escherichia coli, GI1786939, Length=376, Percent_Identity=30.0531914893617, Blast_Score=146, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI17555174, Length=270, Percent_Identity=26.6666666666667, Blast_Score=75, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6319850, Length=258, Percent_Identity=25.5813953488372, Blast_Score=64, Evalue=6e-11,
Organism=Saccharomyces cerevisiae, GI6324328, Length=238, Percent_Identity=25.2100840336134, Blast_Score=64, Evalue=6e-11,
Organism=Drosophila melanogaster, GI21356863, Length=236, Percent_Identity=25.8474576271186, Blast_Score=71, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016142
- InterPro:   IPR016143
- InterPro:   IPR002020
- InterPro:   IPR016141
- InterPro:   IPR019810 [H]

Pfam domain/function: PF00285 Citrate_synt [H]

EC number: =2.3.3.1 [H]

Molecular weight: Translated: 39234; Mature: 39103

Theoretical pI: Translated: 8.26; Mature: 8.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIHAGDRAMTIQLPKSPIGLDGIPAAETVLSHVDGQQGELIIAGHRVTDLAAGTGFEGV
CCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHCCCCCCCEEEECCCHHHHHCCCCCCHH
TARLWSAATRRSLSEADVRVTLGVARQRAFARLPELLAATTGLSIIDGFRAAIAALRPEP
HHHHHHHHHHHCHHHCCEEEEHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCC
GLDDEATIVGACPVIAGALVQRARGAQPIAPNPDVSHASDTLRMVRGDTPAAGEVAALET
CCCCCCEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCHHEEEE
YLVTVSDHGMNASTFAARVVASTQADLFASITAGYCALTGPLHGGAPEPVLEMLDAIGTR
EEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCHHEEECCCCCCCCHHHHHHHHHHCCC
ENIKPWVDAALSRGERLMGFGHRIYQVRDPRADVLKGAIKRLAGSGADLPFASEVEAYIR
CCCHHHHHHHHHCCCHHHHCCHHEEEECCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
QALRQKNPERVLDTNVEFFTAILLDALKIPRQAFTPIFAVARAAGWTAHAREQQRTGRLI
HHHHCCCCHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCEE
RPSSAYIGPKP
CCCCCCCCCCC
>Mature Secondary Structure 
SIHAGDRAMTIQLPKSPIGLDGIPAAETVLSHVDGQQGELIIAGHRVTDLAAGTGFEGV
CCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHCCCCCCCEEEECCCHHHHHCCCCCCHH
TARLWSAATRRSLSEADVRVTLGVARQRAFARLPELLAATTGLSIIDGFRAAIAALRPEP
HHHHHHHHHHHCHHHCCEEEEHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCC
GLDDEATIVGACPVIAGALVQRARGAQPIAPNPDVSHASDTLRMVRGDTPAAGEVAALET
CCCCCCEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCHHEEEE
YLVTVSDHGMNASTFAARVVASTQADLFASITAGYCALTGPLHGGAPEPVLEMLDAIGTR
EEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCHHEEECCCCCCCCHHHHHHHHHHCCC
ENIKPWVDAALSRGERLMGFGHRIYQVRDPRADVLKGAIKRLAGSGADLPFASEVEAYIR
CCCHHHHHHHHHCCCHHHHCCHHEEEECCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
QALRQKNPERVLDTNVEFFTAILLDALKIPRQAFTPIFAVARAAGWTAHAREQQRTGRLI
HHHHCCCCHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCEE
RPSSAYIGPKP
CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8045898; 9579061; 9384377 [H]