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The map label for this gene is phaZ1 [H]

Identifier: 146337643

GI number: 146337643

Start: 514027

End: 515520

Strand: Direct

Name: phaZ1 [H]

Synonym: BRADO0509

Alternate gene names: 146337643

Gene position: 514027-515520 (Clockwise)

Preceding gene: 146337641

Following gene: 146337645

Centisome position: 6.89

GC content: 67.2

Gene sequence:

>1494_bases
TTGCCGAAAGACGAGACCGCCAAGGAGCCGCATTTGTCCCTCGCTGAAAATGTTGAATATTTGCGGCGGCTGCGGGAGCT
GAACGGCATCACCGGGTTCGGCGACTTCCATCGTCAGCTGCGACCCGCACCTGAGTGCCCGCTGGTCGAGGTCGAAGAGT
TCGGCAGCAATCCCGGCCGGCTGAAAATGTTCGCCTATGTGCCGGCGCAGCGCCAGCCGCTGCTGCCGCTCGTCGTCGTG
CTGCATGGCTGCGGGCAGGGCGCCGCGGAGTATGATCTCGGCGCCGGCTGGTCGACGCTCGCAAAACATTTCGGCTTCGC
GCTGCTGATGCCGGAGCAGCAGCGCATCAACAATCCGCAGCGCTGCTTCAACTGGTTTCAGTCCGAGGACATCACGCGCG
GTCAGGGCGAGGTCGCCTCGATCCGCGAGATGATCGCGCGCATGGTGGCCGACTGCGCCATCGATTCCAACCGCATCTTC
GTCACCGGCCTGTCGGCGGGCGGCGCGATGACGATGGCGATGCTGTCGGCGCATCCGGACCTGTTCGCGGCTGGCGCGGT
CATCGCCGGGCTGCCGTTCGGCACGGCGCGCAACATGCGCGATGCGATCCTGCAGATGCGGATGCCGCCGGCGCGTCCCG
CCGGCGAGCTCGGCGATCTCGTGCGCCGCGCCTCCCACCATCGCGGCAAATGGCCGAAACTGTCGGTGTGGCACGGCACC
GCCGACTACACCGTGCATCCGGACAATGCCGGCGAGATCGTCAAGCAATGGCTCGATCTGCATCATCTGCCGCTGGCGCC
GATGGCCGCGAACGAGGTCAACGGCTATCCGCATGAGCAGTGGTGGAACTCCGACGGCGTCACGGTGGTGGAATCCTTTA
CCATCACCAACATGGCGCATGGCACGCCGATCGGCGTCGCCGCCAACGACAAGCGCTACGGCAAGGCCGGGCCGTTCCTG
CTCGAGGCCGGCATCTCCTCGTCCTATCTGATCGCGAAATTCTTCGGCGTTACCGACTGGATCCGGCAGCCCAAGCCGGC
GGTGACCTCGCCGAGCACCAGGCTGATTCCCGAGGTGTCGCCGATCTCGGTGCTGCCGACGCTGACCAAGATGCTGCGGC
GGCCCGAGGATGCCGCCGAGCCGGTGGAGGCCAAGGCTTCGCCCGAGCCAGTGAAAACAGCCAAGCCGGCACGCGCGAAG
TCGAACCGCGCGGCGAGGCCGCCGAAGCGCAAGGTAGATGCTTCCACCGCAGAGCCCGGCGAGATCAAGGCGCTCAGCGC
AACACCTGCCGACGCGCCGGCCGAGGTGAAGCCGCCCGTTGCCAGACCGCCGGTGGCCAAAGCTGTCGACATGACGCCGG
CCGATCCGCCGCCGGTCATCGCGGTGAAGGCGCCGCCGAGTCCCGCCGAGCTGCCGCCTACTCCAAAGGCCCGACGCGTG
ATCGACGTCGCCGCCGTGATCGACCGCGCGCTGAAAGCAGCGGGGCTGAAATAG

Upstream 100 bases:

>100_bases
CGCGTTCATGCGGGCTTTTTCTTTTGCGCTGCAGCAACCATCTGGGTGATGGTGGAGTTGATCCGCGCTGACGTGAAGAC
CTGACCGCCGAAAGACGAGA

Downstream 100 bases:

>100_bases
GCTTTAGACGCGGAACATCTGAGGGACATCGGTTGATCGTCAAAGCCGTCATTCCGGGATGGTCCGAAGGACCAGACATC
GGATGCGCGATTGCGCATCT

Product: putative polyhydroxy-alkanoate/butyrate(PHA/PHB) depolymerase

Products: NA

Alternate protein names: PHA depolymerase; PHB depolymerase [H]

Number of amino acids: Translated: 497; Mature: 496

Protein sequence:

>497_residues
MPKDETAKEPHLSLAENVEYLRRLRELNGITGFGDFHRQLRPAPECPLVEVEEFGSNPGRLKMFAYVPAQRQPLLPLVVV
LHGCGQGAAEYDLGAGWSTLAKHFGFALLMPEQQRINNPQRCFNWFQSEDITRGQGEVASIREMIARMVADCAIDSNRIF
VTGLSAGGAMTMAMLSAHPDLFAAGAVIAGLPFGTARNMRDAILQMRMPPARPAGELGDLVRRASHHRGKWPKLSVWHGT
ADYTVHPDNAGEIVKQWLDLHHLPLAPMAANEVNGYPHEQWWNSDGVTVVESFTITNMAHGTPIGVAANDKRYGKAGPFL
LEAGISSSYLIAKFFGVTDWIRQPKPAVTSPSTRLIPEVSPISVLPTLTKMLRRPEDAAEPVEAKASPEPVKTAKPARAK
SNRAARPPKRKVDASTAEPGEIKALSATPADAPAEVKPPVARPPVAKAVDMTPADPPPVIAVKAPPSPAELPPTPKARRV
IDVAAVIDRALKAAGLK

Sequences:

>Translated_497_residues
MPKDETAKEPHLSLAENVEYLRRLRELNGITGFGDFHRQLRPAPECPLVEVEEFGSNPGRLKMFAYVPAQRQPLLPLVVV
LHGCGQGAAEYDLGAGWSTLAKHFGFALLMPEQQRINNPQRCFNWFQSEDITRGQGEVASIREMIARMVADCAIDSNRIF
VTGLSAGGAMTMAMLSAHPDLFAAGAVIAGLPFGTARNMRDAILQMRMPPARPAGELGDLVRRASHHRGKWPKLSVWHGT
ADYTVHPDNAGEIVKQWLDLHHLPLAPMAANEVNGYPHEQWWNSDGVTVVESFTITNMAHGTPIGVAANDKRYGKAGPFL
LEAGISSSYLIAKFFGVTDWIRQPKPAVTSPSTRLIPEVSPISVLPTLTKMLRRPEDAAEPVEAKASPEPVKTAKPARAK
SNRAARPPKRKVDASTAEPGEIKALSATPADAPAEVKPPVARPPVAKAVDMTPADPPPVIAVKAPPSPAELPPTPKARRV
IDVAAVIDRALKAAGLK
>Mature_496_residues
PKDETAKEPHLSLAENVEYLRRLRELNGITGFGDFHRQLRPAPECPLVEVEEFGSNPGRLKMFAYVPAQRQPLLPLVVVL
HGCGQGAAEYDLGAGWSTLAKHFGFALLMPEQQRINNPQRCFNWFQSEDITRGQGEVASIREMIARMVADCAIDSNRIFV
TGLSAGGAMTMAMLSAHPDLFAAGAVIAGLPFGTARNMRDAILQMRMPPARPAGELGDLVRRASHHRGKWPKLSVWHGTA
DYTVHPDNAGEIVKQWLDLHHLPLAPMAANEVNGYPHEQWWNSDGVTVVESFTITNMAHGTPIGVAANDKRYGKAGPFLL
EAGISSSYLIAKFFGVTDWIRQPKPAVTSPSTRLIPEVSPISVLPTLTKMLRRPEDAAEPVEAKASPEPVKTAKPARAKS
NRAARPPKRKVDASTAEPGEIKALSATPADAPAEVKPPVARPPVAKAVDMTPADPPPVIAVKAPPSPAELPPTPKARRVI
DVAAVIDRALKAAGLK

Specific function: Specific for poly(hydroxyalkanoic acid) consisting of monomers of four or five carbon atoms and for P- nitrophenylbutyrate as substrates [H]

COG id: COG3509

COG function: function code Q; Poly(3-hydroxybutyrate) depolymerase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Lipase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010126 [H]

Pfam domain/function: NA

EC number: 3.1.1.-

Molecular weight: Translated: 53727; Mature: 53596

Theoretical pI: Translated: 9.48; Mature: 9.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKDETAKEPHLSLAENVEYLRRLRELNGITGFGDFHRQLRPAPECPLVEVEEFGSNPGR
CCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCEEEHHHCCCCCCC
LKMFAYVPAQRQPLLPLVVVLHGCGQGAAEYDLGAGWSTLAKHFGFALLMPEQQRINNPQ
EEEEEECCCCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCEEEECCCHHHCCCHH
RCFNWFQSEDITRGQGEVASIREMIARMVADCAIDSNRIFVTGLSAGGAMTMAMLSAHPD
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHCCCC
LFAAGAVIAGLPFGTARNMRDAILQMRMPPARPAGELGDLVRRASHHRGKWPKLSVWHGT
HHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECC
ADYTVHPDNAGEIVKQWLDLHHLPLAPMAANEVNGYPHEQWWNSDGVTVVESFTITNMAH
CCCEECCCCHHHHHHHHHHHHCCCCCCCCHHHCCCCCCHHCCCCCCCEEEEEEEEHHCCC
GTPIGVAANDKRYGKAGPFLLEAGISSSYLIAKFFGVTDWIRQPKPAVTSPSTRLIPEVS
CCCEEEECCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
PISVLPTLTKMLRRPEDAAEPVEAKASPEPVKTAKPARAKSNRAARPPKRKVDASTAEPG
CHHHHHHHHHHHHCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
EIKALSATPADAPAEVKPPVARPPVAKAVDMTPADPPPVIAVKAPPSPAELPPTPKARRV
CEEEEECCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCEEEEECCCCCCCCCCCCHHHHH
IDVAAVIDRALKAAGLK
HHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PKDETAKEPHLSLAENVEYLRRLRELNGITGFGDFHRQLRPAPECPLVEVEEFGSNPGR
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCEEEHHHCCCCCCC
LKMFAYVPAQRQPLLPLVVVLHGCGQGAAEYDLGAGWSTLAKHFGFALLMPEQQRINNPQ
EEEEEECCCCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCEEEECCCHHHCCCHH
RCFNWFQSEDITRGQGEVASIREMIARMVADCAIDSNRIFVTGLSAGGAMTMAMLSAHPD
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHCCCC
LFAAGAVIAGLPFGTARNMRDAILQMRMPPARPAGELGDLVRRASHHRGKWPKLSVWHGT
HHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECC
ADYTVHPDNAGEIVKQWLDLHHLPLAPMAANEVNGYPHEQWWNSDGVTVVESFTITNMAH
CCCEECCCCHHHHHHHHHHHHCCCCCCCCHHHCCCCCCHHCCCCCCCEEEEEEEEHHCCC
GTPIGVAANDKRYGKAGPFLLEAGISSSYLIAKFFGVTDWIRQPKPAVTSPSTRLIPEVS
CCCEEEECCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
PISVLPTLTKMLRRPEDAAEPVEAKASPEPVKTAKPARAKSNRAARPPKRKVDASTAEPG
CHHHHHHHHHHHHCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
EIKALSATPADAPAEVKPPVARPPVAKAVDMTPADPPPVIAVKAPPSPAELPPTPKARRV
CEEEEECCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCEEEEECCCCCCCCCCCCHHHHH
IDVAAVIDRALKAAGLK
HHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8269961 [H]