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The map label for this gene is dapF [H]

Identifier: 146337529

GI number: 146337529

Start: 388384

End: 389253

Strand: Direct

Name: dapF [H]

Synonym: BRADO0383

Alternate gene names: 146337529

Gene position: 388384-389253 (Clockwise)

Preceding gene: 146337526

Following gene: 146337530

Centisome position: 5.21

GC content: 66.78

Gene sequence:

>870_bases
ATGAGCCCGCTGGCCAACCACAGCTTCGTCAAGATGAACGGGATCGGCAACGAAATCGTCGTGCTGGATCTGCGCGACGT
GAAGCATGTCGTCACGCCGGACGAGGCGCGGGCGGTTGCATCGCGCGTACCTTACGACCAGATGATGGTGCTGCAGCGGC
CACGGTTCGATGGCACCGAGGCCTTCATCCGCATCTACAACAATGACGGCTCGGAATCCGGCGCCTGCGGCAACGGCATG
CGCTGCGTGGTGAGCCAGGTGTTCGGCAAGACCGGGCAGACCAGCGCGACGTTCGAGACCCGCGCCGGCCTGTTGAACTG
CTGGCAGGGGCCGGCGCCCGATCTCTACACTGTCGACATGGGCGTGCCGAGGTTCGGCTGGCAGGACATTCCCCTGGCCG
AGGAGTTCCGCGACACCCGCTACATCGAGCTGCAGATCGGCCCGATCGACGCGCCGATCCTGCATTCGCCGTCGGTCGTC
AGCATGGGCAATCCGCACGCTGTGTTCTGGGTCGACAACGACGTCAATTCCTATGACCTCGAGCGCTTCGGGCCGCTCTT
GGAAAACCACCCGATCTTCCCGGAGCGGGCCAACATCACGCTGGCCCAGATCGTCGACCGCGACCACATCACGATGCGCA
CCTGGGAGCGCGGCGCGGGACTGACCCGGGCCTGCGGCTCGGCGGCGTGTGCGACCGCGGTCGCCGCCGCCCGGCTGAAG
CGCGCCAACCGGCTCGTGCACATGACGCTGCCGGGCGGCGAGCTGACGATCGAGTGGCGCGAGCGCGACGACCACGTGTT
GATGACGGGCACCGCGACGTTCGAGTTCGAGGGCCGGTTTGAGCCAGCGCTGTTCGCCAGCGTCGCATGA

Upstream 100 bases:

>100_bases
CGGCGGCGCGGCGTCCCATGACAGATGCGTCATGTCCCCCTGCGGACTGGTCAATGCGGCGCCTTTCGCCATATAAGGCG
GACACAATTTGGATATTTTC

Downstream 100 bases:

>100_bases
GCGTCGATGTCGTCACCTTCGGCTGCCGGCTCAACGCGTTCGAGTCCGAGCTGATCGCGCGCCATGTCGAGGCAGCGGGG
GCGGACGACACCATCGTCAT

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase [H]

Number of amino acids: Translated: 289; Mature: 288

Protein sequence:

>289_residues
MSPLANHSFVKMNGIGNEIVVLDLRDVKHVVTPDEARAVASRVPYDQMMVLQRPRFDGTEAFIRIYNNDGSESGACGNGM
RCVVSQVFGKTGQTSATFETRAGLLNCWQGPAPDLYTVDMGVPRFGWQDIPLAEEFRDTRYIELQIGPIDAPILHSPSVV
SMGNPHAVFWVDNDVNSYDLERFGPLLENHPIFPERANITLAQIVDRDHITMRTWERGAGLTRACGSAACATAVAAARLK
RANRLVHMTLPGGELTIEWRERDDHVLMTGTATFEFEGRFEPALFASVA

Sequences:

>Translated_289_residues
MSPLANHSFVKMNGIGNEIVVLDLRDVKHVVTPDEARAVASRVPYDQMMVLQRPRFDGTEAFIRIYNNDGSESGACGNGM
RCVVSQVFGKTGQTSATFETRAGLLNCWQGPAPDLYTVDMGVPRFGWQDIPLAEEFRDTRYIELQIGPIDAPILHSPSVV
SMGNPHAVFWVDNDVNSYDLERFGPLLENHPIFPERANITLAQIVDRDHITMRTWERGAGLTRACGSAACATAVAAARLK
RANRLVHMTLPGGELTIEWRERDDHVLMTGTATFEFEGRFEPALFASVA
>Mature_288_residues
SPLANHSFVKMNGIGNEIVVLDLRDVKHVVTPDEARAVASRVPYDQMMVLQRPRFDGTEAFIRIYNNDGSESGACGNGMR
CVVSQVFGKTGQTSATFETRAGLLNCWQGPAPDLYTVDMGVPRFGWQDIPLAEEFRDTRYIELQIGPIDAPILHSPSVVS
MGNPHAVFWVDNDVNSYDLERFGPLLENHPIFPERANITLAQIVDRDHITMRTWERGAGLTRACGSAACATAVAAARLKR
ANRLVHMTLPGGELTIEWRERDDHVLMTGTATFEFEGRFEPALFASVA

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family [H]

Homologues:

Organism=Escherichia coli, GI87082334, Length=276, Percent_Identity=37.6811594202899, Blast_Score=163, Evalue=1e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001653
- InterPro:   IPR018510 [H]

Pfam domain/function: PF01678 DAP_epimerase [H]

EC number: =5.1.1.7 [H]

Molecular weight: Translated: 32005; Mature: 31873

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPLANHSFVKMNGIGNEIVVLDLRDVKHVVTPDEARAVASRVPYDQMMVLQRPRFDGTE
CCCCCCCCEEEECCCCCEEEEEEECCCCEECCCHHHHHHHHHCCHHHHHHEECCCCCCCE
AFIRIYNNDGSESGACGNGMRCVVSQVFGKTGQTSATFETRAGLLNCWQGPAPDLYTVDM
EEEEEECCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCCEEEEEC
GVPRFGWQDIPLAEEFRDTRYIELQIGPIDAPILHSPSVVSMGNPHAVFWVDNDVNSYDL
CCCCCCCCCCCCHHHHCCCEEEEEEECCCCCCCCCCCCEEECCCCEEEEEEECCCCCCCH
ERFGPLLENHPIFPERANITLAQIVDRDHITMRTWERGAGLTRACGSAACATAVAAARLK
HHHCHHHHCCCCCCCCCCEEEEHHHCCCCEEEEEECCCCCHHHHHCHHHHHHHHHHHHHH
RANRLVHMTLPGGELTIEWRERDDHVLMTGTATFEFEGRFEPALFASVA
HCCCEEEEEECCCEEEEEEECCCCEEEEEEEEEEEECCCCCCCEEEECC
>Mature Secondary Structure 
SPLANHSFVKMNGIGNEIVVLDLRDVKHVVTPDEARAVASRVPYDQMMVLQRPRFDGTE
CCCCCCCEEEECCCCCEEEEEEECCCCEECCCHHHHHHHHHCCHHHHHHEECCCCCCCE
AFIRIYNNDGSESGACGNGMRCVVSQVFGKTGQTSATFETRAGLLNCWQGPAPDLYTVDM
EEEEEECCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCCEEEEEC
GVPRFGWQDIPLAEEFRDTRYIELQIGPIDAPILHSPSVVSMGNPHAVFWVDNDVNSYDL
CCCCCCCCCCCCHHHHCCCEEEEEEECCCCCCCCCCCCEEECCCCEEEEEEECCCCCCCH
ERFGPLLENHPIFPERANITLAQIVDRDHITMRTWERGAGLTRACGSAACATAVAAARLK
HHHCHHHHCCCCCCCCCCEEEEHHHCCCCEEEEEECCCCCHHHHHCHHHHHHHHHHHHHH
RANRLVHMTLPGGELTIEWRERDDHVLMTGTATFEFEGRFEPALFASVA
HCCCEEEEEECCCEEEEEEECCCCEEEEEEEEEEEECCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA