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The map label for this gene is leuD [H]

Identifier: 146337514

GI number: 146337514

Start: 375166

End: 375777

Strand: Reverse

Name: leuD [H]

Synonym: BRADO0365

Alternate gene names: 146337514

Gene position: 375777-375166 (Counterclockwise)

Preceding gene: 146337515

Following gene: 146337513

Centisome position: 5.04

GC content: 63.73

Gene sequence:

>612_bases
GTGCCCCCGATGGACAAGTTCACCACGCTGGAAGGCGTCGCCGCGCCGCTGAAGATCATCAATGTCGATACCGACATGAT
CATCCCCAAGCAGTACCTGAAGACCATCAAGCGCACCGGTCTCGGCAAGGGCCTGTTCTCGGAGCAGCGCTACAAGGACG
ACGGCAGCGAGAACCCCGATTTCGTGCTCAACCAGCCGGCCTATCGCAACGCCAAGATCCTGGTCGCCGGGGACAATTTC
GGCTGCGGCTCGAGCCGCGAGCACGCCCCCTGGGCGCTGCTCGATTTCGGCATCCGCTGCGTGATCTCGACCTCGTTCGG
CGACATCTTCTACAACAACTGCTTCAAGAACGGCATCCTGCCGATCCGTGTCTCGCAGGCCGATCTCGACAAGCTGTTCG
ACGACGCCGAGCGCGGCGCCAATGCGACGCTCACGATCGATCTCGCCGCCCAGGAGATCCGGGGCCCCGACGGCGGCACC
GTGAAGTTCGAGATCGACCCGTTCCGCAAGCACTGCCTGCTCAACGGGCTCGACGACATCGGGCTGACGCTGGAAAAGAA
GGCCTCGATCGACAGCTACGAGGCCAAGCTCGCCGAGCGCGCCTGGGCCTGA

Upstream 100 bases:

>100_bases
GGCCGCCGCCGACTGACCTGGACGGCTGGATTTTCGGGCAATTTGACTTTATCCGCCCCGCCAATCGGGCTAAACAGCGG
CCTGCCCGATACTCTCGAAG

Downstream 100 bases:

>100_bases
GCCCCCGCCTGACAGCACAGAGTTCGCGGGCCCCGGACCTCGTCCGGGGCCTTGTTTCGAGAGCATCCCGAGCACAGGTC
CGCCGTTGCCGCGCGTATTG

Product: isopropylmalate isomerase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 203; Mature: 202

Protein sequence:

>203_residues
MPPMDKFTTLEGVAAPLKIINVDTDMIIPKQYLKTIKRTGLGKGLFSEQRYKDDGSENPDFVLNQPAYRNAKILVAGDNF
GCGSSREHAPWALLDFGIRCVISTSFGDIFYNNCFKNGILPIRVSQADLDKLFDDAERGANATLTIDLAAQEIRGPDGGT
VKFEIDPFRKHCLLNGLDDIGLTLEKKASIDSYEAKLAERAWA

Sequences:

>Translated_203_residues
MPPMDKFTTLEGVAAPLKIINVDTDMIIPKQYLKTIKRTGLGKGLFSEQRYKDDGSENPDFVLNQPAYRNAKILVAGDNF
GCGSSREHAPWALLDFGIRCVISTSFGDIFYNNCFKNGILPIRVSQADLDKLFDDAERGANATLTIDLAAQEIRGPDGGT
VKFEIDPFRKHCLLNGLDDIGLTLEKKASIDSYEAKLAERAWA
>Mature_202_residues
PPMDKFTTLEGVAAPLKIINVDTDMIIPKQYLKTIKRTGLGKGLFSEQRYKDDGSENPDFVLNQPAYRNAKILVAGDNFG
CGSSREHAPWALLDFGIRCVISTSFGDIFYNNCFKNGILPIRVSQADLDKLFDDAERGANATLTIDLAAQEIRGPDGGTV
KFEIDPFRKHCLLNGLDDIGLTLEKKASIDSYEAKLAERAWA

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786258, Length=194, Percent_Identity=51.0309278350515, Blast_Score=208, Evalue=2e-55,
Organism=Saccharomyces cerevisiae, GI6321429, Length=204, Percent_Identity=59.3137254901961, Blast_Score=228, Evalue=4e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004431
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573
- InterPro:   IPR015936 [H]

Pfam domain/function: PF00694 Aconitase_C [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 22433; Mature: 22302

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPMDKFTTLEGVAAPLKIINVDTDMIIPKQYLKTIKRTGLGKGLFSEQRYKDDGSENPD
CCCCCCHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHCCCCCCCCCCHHHCCCCCCCCC
FVLNQPAYRNAKILVAGDNFGCGSSREHAPWALLDFGIRCVISTSFGDIFYNNCFKNGIL
EEECCCCCCCCEEEEECCCCCCCCCCCCCCCHHEECCEEEEEECCCCHHHHHHHHHCCCE
PIRVSQADLDKLFDDAERGANATLTIDLAAQEIRGPDGGTVKFEIDPFRKHCLLNGLDDI
EEEECHHHHHHHHHHHHCCCCEEEEEEEEHHHHCCCCCCEEEEEECHHHHHHHHCCCCCC
GLTLEKKASIDSYEAKLAERAWA
CEEEECCCCCCHHHHHHHHHCCC
>Mature Secondary Structure 
PPMDKFTTLEGVAAPLKIINVDTDMIIPKQYLKTIKRTGLGKGLFSEQRYKDDGSENPD
CCCCCHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHCCCCCCCCCCHHHCCCCCCCCC
FVLNQPAYRNAKILVAGDNFGCGSSREHAPWALLDFGIRCVISTSFGDIFYNNCFKNGIL
EEECCCCCCCCEEEEECCCCCCCCCCCCCCCHHEECCEEEEEECCCCHHHHHHHHHCCCE
PIRVSQADLDKLFDDAERGANATLTIDLAAQEIRGPDGGTVKFEIDPFRKHCLLNGLDDI
EEEECHHHHHHHHHHHHCCCCEEEEEEEEHHHHCCCCCCEEEEEECHHHHHHHHCCCCCC
GLTLEKKASIDSYEAKLAERAWA
CEEEECCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]