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The map label for this gene is leuB [H]

Identifier: 146337504

GI number: 146337504

Start: 365614

End: 366726

Strand: Direct

Name: leuB [H]

Synonym: BRADO0353

Alternate gene names: 146337504

Gene position: 365614-366726 (Clockwise)

Preceding gene: 146337502

Following gene: 146337507

Centisome position: 4.9

GC content: 66.94

Gene sequence:

>1113_bases
ATGGCGACCCACAAGCTCCTGCTGCTGCCCGGCGACGGCATCGGCCCCGAAGTGATGGCCGAGGTGAAGCGGCTGATCGG
CTGGCTCAACGGCAAGGGCCTGGCCTCGTTCGAGACCGAGGACGGTTTGGTCGGCGGCGCCGCCTATGACGCGCACGGCG
CCTCGATCTCGGAAGGAGACATGGACAAGGCCAAGGCGGCCGACGCCATCATCTTCGGCGCCGTTGGCGGTCCGAAGTGG
GACGGCGTTCCCTACGAGGTGCGTCCCGAGGCCGGCCTGCTCCGCCTGCGCAAGGATCTCGGCCTGTTCGCCAACCTGCG
TCCCGCCGTCTGCTATCCGGCGCTCGCCGACGCCTCCAGCCTGAAGCGCGAGATCGTCGAGGGTCTCGACATCATGATCG
TGCGCGAGCTCACCGGAGGCGTGTATTTCGGCGAGCCGAAGACCATCACCGATCTCGGCAACGGCCAGAAGCGCGCGATC
GACACCCAGGTCTACGACACCTATGAGATCGAACGCATCGCCCGCGTCGCCTTCGACCTCGCGCGCAAGCGCAAGAACAA
GGTGACGTCGATGGAGAAGCGCAACGTGATGAAGTCGGGCGTGCTCTGGAACGAGGTCGTCACCGCGGTCCACGCGCGCG
AGTACAAGGACGTCACGCTCGAGCACCAGCTCGCCGATTCCGGCGGCATGAACCTAGTGAAATATCCCAAGATGTTCGAC
GTCATCGTCACCGACAACCTGTTCGGCGACATGCTGTCCGACATCGCCGCGATGCTGACCGGCTCGCTCGGCATGCTGCC
GTCGGCCTCGCTCGGCGAGGTCGACGCCAAGACCCACAAGCGCCGCGCGCTGTATGAGCCGGTGCACGGCTCGGCACCGG
ACATTGCAGGCCGAGGCCTCGCCAACCCGATCGCGATGCTGACCTCCTTCGGCATGGCGCTGCGCTACTCGTTCGACCTC
GGCGCGCTGGCCGACAAGCTCGACGCCGCCATCGCCGCCGTGCTGGCGAGCGGCCTGCGCACCGCCGACATCAAGGCCGA
GGGCTCGACGCCCGCGTCGACCACGCAGATGGGCGAGGCGATCATCAAGGAGCTCGAGACGCTCACGGCGTAA

Upstream 100 bases:

>100_bases
GCCGCTGCCGCCGATCCGATCGGCGGCAAGCCGGCAATCCCAAGCATGATGGTTGCCCGCGCAACGCCCGGGCATGACCA
GCCCTACGAGGACAACGACC

Downstream 100 bases:

>100_bases
TTTGCCCCATGCTCATTTGAGCCAAGCAATCTCCGCGCGGACGCTCCGCTCCCCTCCCCCTTGCGGGGAGGGCTATCGCA
TATGGCGATGCGGAGCTGTA

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH 2; Beta-IPM dehydrogenase 2; IMDH 2 [H]

Number of amino acids: Translated: 370; Mature: 369

Protein sequence:

>370_residues
MATHKLLLLPGDGIGPEVMAEVKRLIGWLNGKGLASFETEDGLVGGAAYDAHGASISEGDMDKAKAADAIIFGAVGGPKW
DGVPYEVRPEAGLLRLRKDLGLFANLRPAVCYPALADASSLKREIVEGLDIMIVRELTGGVYFGEPKTITDLGNGQKRAI
DTQVYDTYEIERIARVAFDLARKRKNKVTSMEKRNVMKSGVLWNEVVTAVHAREYKDVTLEHQLADSGGMNLVKYPKMFD
VIVTDNLFGDMLSDIAAMLTGSLGMLPSASLGEVDAKTHKRRALYEPVHGSAPDIAGRGLANPIAMLTSFGMALRYSFDL
GALADKLDAAIAAVLASGLRTADIKAEGSTPASTTQMGEAIIKELETLTA

Sequences:

>Translated_370_residues
MATHKLLLLPGDGIGPEVMAEVKRLIGWLNGKGLASFETEDGLVGGAAYDAHGASISEGDMDKAKAADAIIFGAVGGPKW
DGVPYEVRPEAGLLRLRKDLGLFANLRPAVCYPALADASSLKREIVEGLDIMIVRELTGGVYFGEPKTITDLGNGQKRAI
DTQVYDTYEIERIARVAFDLARKRKNKVTSMEKRNVMKSGVLWNEVVTAVHAREYKDVTLEHQLADSGGMNLVKYPKMFD
VIVTDNLFGDMLSDIAAMLTGSLGMLPSASLGEVDAKTHKRRALYEPVHGSAPDIAGRGLANPIAMLTSFGMALRYSFDL
GALADKLDAAIAAVLASGLRTADIKAEGSTPASTTQMGEAIIKELETLTA
>Mature_369_residues
ATHKLLLLPGDGIGPEVMAEVKRLIGWLNGKGLASFETEDGLVGGAAYDAHGASISEGDMDKAKAADAIIFGAVGGPKWD
GVPYEVRPEAGLLRLRKDLGLFANLRPAVCYPALADASSLKREIVEGLDIMIVRELTGGVYFGEPKTITDLGNGQKRAID
TQVYDTYEIERIARVAFDLARKRKNKVTSMEKRNVMKSGVLWNEVVTAVHAREYKDVTLEHQLADSGGMNLVKYPKMFDV
IVTDNLFGDMLSDIAAMLTGSLGMLPSASLGEVDAKTHKRRALYEPVHGSAPDIAGRGLANPIAMLTSFGMALRYSFDLG
ALADKLDAAIAAVLASGLRTADIKAEGSTPASTTQMGEAIIKELETLTA

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=340, Percent_Identity=33.5294117647059, Blast_Score=131, Evalue=8e-31,
Organism=Homo sapiens, GI28178821, Length=367, Percent_Identity=28.3378746594005, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI28178816, Length=315, Percent_Identity=29.5238095238095, Blast_Score=102, Evalue=4e-22,
Organism=Homo sapiens, GI4758582, Length=354, Percent_Identity=28.5310734463277, Blast_Score=102, Evalue=4e-22,
Organism=Homo sapiens, GI28178838, Length=336, Percent_Identity=29.1666666666667, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI28178819, Length=202, Percent_Identity=32.6732673267327, Blast_Score=82, Evalue=1e-15,
Organism=Escherichia coli, GI87081683, Length=364, Percent_Identity=47.8021978021978, Blast_Score=324, Evalue=7e-90,
Organism=Escherichia coli, GI1788101, Length=374, Percent_Identity=34.7593582887701, Blast_Score=174, Evalue=6e-45,
Organism=Escherichia coli, GI1787381, Length=314, Percent_Identity=23.8853503184713, Blast_Score=65, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI71986051, Length=370, Percent_Identity=30.5405405405405, Blast_Score=134, Evalue=8e-32,
Organism=Caenorhabditis elegans, GI17505779, Length=356, Percent_Identity=30.0561797752809, Blast_Score=124, Evalue=7e-29,
Organism=Caenorhabditis elegans, GI25144293, Length=373, Percent_Identity=28.4182305630027, Blast_Score=122, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI17550882, Length=367, Percent_Identity=29.700272479564, Blast_Score=112, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6319830, Length=374, Percent_Identity=39.8395721925134, Blast_Score=251, Evalue=9e-68,
Organism=Saccharomyces cerevisiae, GI6322097, Length=354, Percent_Identity=34.4632768361582, Blast_Score=166, Evalue=5e-42,
Organism=Saccharomyces cerevisiae, GI6324709, Length=370, Percent_Identity=31.8918918918919, Blast_Score=129, Evalue=8e-31,
Organism=Saccharomyces cerevisiae, GI6324291, Length=373, Percent_Identity=28.4182305630027, Blast_Score=114, Evalue=3e-26,
Organism=Drosophila melanogaster, GI24643270, Length=343, Percent_Identity=33.8192419825073, Blast_Score=140, Evalue=1e-33,
Organism=Drosophila melanogaster, GI24643268, Length=343, Percent_Identity=33.8192419825073, Blast_Score=140, Evalue=1e-33,
Organism=Drosophila melanogaster, GI24661184, Length=374, Percent_Identity=32.3529411764706, Blast_Score=121, Evalue=6e-28,
Organism=Drosophila melanogaster, GI161078635, Length=382, Percent_Identity=26.7015706806283, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI161078637, Length=382, Percent_Identity=26.7015706806283, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI161078633, Length=382, Percent_Identity=26.7015706806283, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24650122, Length=382, Percent_Identity=26.7015706806283, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI161078639, Length=378, Percent_Identity=26.7195767195767, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI281362242, Length=350, Percent_Identity=27.4285714285714, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24648872, Length=350, Percent_Identity=27.4285714285714, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI20130355, Length=356, Percent_Identity=26.4044943820225, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 39552; Mature: 39421

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATHKLLLLPGDGIGPEVMAEVKRLIGWLNGKGLASFETEDGLVGGAAYDAHGASISEGD
CCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEECCCEECCCCCCCCCCC
MDKAKAADAIIFGAVGGPKWDGVPYEVRPEAGLLRLRKDLGLFANLRPAVCYPALADASS
CHHHHHCCEEEEECCCCCCCCCCCEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHCCHHH
LKREIVEGLDIMIVRELTGGVYFGEPKTITDLGNGQKRAIDTQVYDTYEIERIARVAFDL
HHHHHHCCCCEEEEEECCCCEEECCCCEEECCCCCCHHEECHHHHHHHHHHHHHHHHHHH
ARKRKNKVTSMEKRNVMKSGVLWNEVVTAVHAREYKDVTLEHQLADSGGMNLVKYPKMFD
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCEEECCCCEE
VIVTDNLFGDMLSDIAAMLTGSLGMLPSASLGEVDAKTHKRRALYEPVHGSAPDIAGRGL
EEEECHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCC
ANPIAMLTSFGMALRYSFDLGALADKLDAAIAAVLASGLRTADIKAEGSTPASTTQMGEA
CHHHHHHHHHHHHEEEECCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHH
IIKELETLTA
HHHHHHHHCC
>Mature Secondary Structure 
ATHKLLLLPGDGIGPEVMAEVKRLIGWLNGKGLASFETEDGLVGGAAYDAHGASISEGD
CCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEECCCEECCCCCCCCCCC
MDKAKAADAIIFGAVGGPKWDGVPYEVRPEAGLLRLRKDLGLFANLRPAVCYPALADASS
CHHHHHCCEEEEECCCCCCCCCCCEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHCCHHH
LKREIVEGLDIMIVRELTGGVYFGEPKTITDLGNGQKRAIDTQVYDTYEIERIARVAFDL
HHHHHHCCCCEEEEEECCCCEEECCCCEEECCCCCCHHEECHHHHHHHHHHHHHHHHHHH
ARKRKNKVTSMEKRNVMKSGVLWNEVVTAVHAREYKDVTLEHQLADSGGMNLVKYPKMFD
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCEEECCCCEE
VIVTDNLFGDMLSDIAAMLTGSLGMLPSASLGEVDAKTHKRRALYEPVHGSAPDIAGRGL
EEEECHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCC
ANPIAMLTSFGMALRYSFDLGALADKLDAAIAAVLASGLRTADIKAEGSTPASTTQMGEA
CHHHHHHHHHHHHEEEECCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHH
IIKELETLTA
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]