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The map label for this gene is rsmB [H]

Identifier: 146337494

GI number: 146337494

Start: 354869

End: 356221

Strand: Reverse

Name: rsmB [H]

Synonym: BRADO0340

Alternate gene names: 146337494

Gene position: 356221-354869 (Counterclockwise)

Preceding gene: 146337496

Following gene: 146337493

Centisome position: 4.78

GC content: 70.21

Gene sequence:

>1353_bases
ATGCCCCCGCCGCGTTTTGCGACACCTTCCGAAGTCCCGGGCCTTGCGGCACGACGTATCGCCGCCGACGTGATCGACGG
CGTGCTGCACAAGCATCGCACGCTCGACGATCAGCTCGACGGCCCCGGCGCGCATCCCGGACTGAAGCAGTTGGCCGATC
GCGACCGTGCGCTGATGCGCCGGCTGGTTGCGACCACGCTCCGCCGCCTCGGGACGCTTGGAAACGTGCTGTCGCGGCTG
TTGGATCGCGGCATCCCGACCGACGCCCCGCGCGCGCAAAGCGCACTCTTGATCGGCGCCGCGCAGATCCTGTGGATGGA
CGTGCCCGACCACGCCGCCGTCGATCTGTCAGTGCGGCTGGTCCAGGCCGACAGGCGCGCGGCGAAGTATGCCGGCCTGG
TCAATGCGGTGCTGCGCCGTTGCGCCCGCGAGGGAGCGGCCCTGCTCGATGATGCCAAAGGCCAGCCGCTCGACATCGCG
CCCTGGCTGCTGAAGCGCTGGACCGATCATTATGGCGAGGCCACCGCCAGGGACATCGCAGCTGCGCTCGGCCAGGAGCC
GTCGCTCGACCTCACCGTCAAGTCAGACCCAGCGCAATGGGCCTCGCGGCTGCACGGCGAGGTGCTGCCAACCGGAACCG
TCCGGACGCTTCTGCATGGCTCGGTGACGGTGCTGCCAGGTTTTGCCGAGGGTCAATGGTGGGTGCAGGACGCGGCCGCC
GCGCTGCCCGCCCGCTTGTTTGGCGACGTCGCCGGCAAGTCCATGGCGGACCTCTGCGCCGCTCCCGGCGGCAAGACCGC
GCAGCTCGCCCATGGCGGCGCCCGTGTCACGGCCGTCGACAGGTCGCCGGCCCGGATGAGCCGGCTAAAGGACAACATGA
CGCGGTTGCAGCTGCCGGCTGAGACCGTGGTCACCGACGCCGCCGAATGGCAGCCCGCGGACGCCGGTGGCTTCGACGGC
ATCCTGCTCGACGCGCCCTGCACTTCGACCGGAACGATCCGCAGGCATCCCGATGTCGGCTGGCTGCGCAACGAGGCCGA
TATCGCCGCGCTGTCCGGAACGCAGGTGCGGCTGCTGACGCGGGCCGCCGGACTCCTCAAGCCGGGGGGGACCCTGGTCT
ACTGCACCTGCTCGTTGGAGCCCGAGGAGGGCGAACAGGTGGTGGACCATGTTCTCGCCGCCGGCACGGAACTTCGGCGC
GTTCCGATCGATCCGGCCGAGGTGGCGGGACTGGCCGAGCTGGTGACGCCGATGGGCGATCTGCGAACCCTGCCCTGCCA
TTTGCCGCACGCCGATCCGCGGCTAGGCGGTCTCGACGGTTTTTACGCCGCGAGGCTGGAGCGGCGGCCATGA

Upstream 100 bases:

>100_bases
TACATTCGGCATATTCCGGTGGCAGAGGACCCGCAAGCTGATGGCATGCACCCGATCGTCGCGGAACTGCCCTTCTTCTT
TTCGCGAGTAGCCTCCAGGC

Downstream 100 bases:

>100_bases
GCATGGCATCGTGGGCGCAAGGTATGGCTGCGCCGTGGCGGTAGCCGATTGAGGGCGTCGGCGTTGCGTTAGTCGTTAAA
TCCTGATTTTGCACCAGTTT

Product: putative ribosomal RNA small subunit methyltransferase B

Products: NA

Alternate protein names: 16S rRNA m5C967 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase rsmB [H]

Number of amino acids: Translated: 450; Mature: 449

Protein sequence:

>450_residues
MPPPRFATPSEVPGLAARRIAADVIDGVLHKHRTLDDQLDGPGAHPGLKQLADRDRALMRRLVATTLRRLGTLGNVLSRL
LDRGIPTDAPRAQSALLIGAAQILWMDVPDHAAVDLSVRLVQADRRAAKYAGLVNAVLRRCAREGAALLDDAKGQPLDIA
PWLLKRWTDHYGEATARDIAAALGQEPSLDLTVKSDPAQWASRLHGEVLPTGTVRTLLHGSVTVLPGFAEGQWWVQDAAA
ALPARLFGDVAGKSMADLCAAPGGKTAQLAHGGARVTAVDRSPARMSRLKDNMTRLQLPAETVVTDAAEWQPADAGGFDG
ILLDAPCTSTGTIRRHPDVGWLRNEADIAALSGTQVRLLTRAAGLLKPGGTLVYCTCSLEPEEGEQVVDHVLAAGTELRR
VPIDPAEVAGLAELVTPMGDLRTLPCHLPHADPRLGGLDGFYAARLERRP

Sequences:

>Translated_450_residues
MPPPRFATPSEVPGLAARRIAADVIDGVLHKHRTLDDQLDGPGAHPGLKQLADRDRALMRRLVATTLRRLGTLGNVLSRL
LDRGIPTDAPRAQSALLIGAAQILWMDVPDHAAVDLSVRLVQADRRAAKYAGLVNAVLRRCAREGAALLDDAKGQPLDIA
PWLLKRWTDHYGEATARDIAAALGQEPSLDLTVKSDPAQWASRLHGEVLPTGTVRTLLHGSVTVLPGFAEGQWWVQDAAA
ALPARLFGDVAGKSMADLCAAPGGKTAQLAHGGARVTAVDRSPARMSRLKDNMTRLQLPAETVVTDAAEWQPADAGGFDG
ILLDAPCTSTGTIRRHPDVGWLRNEADIAALSGTQVRLLTRAAGLLKPGGTLVYCTCSLEPEEGEQVVDHVLAAGTELRR
VPIDPAEVAGLAELVTPMGDLRTLPCHLPHADPRLGGLDGFYAARLERRP
>Mature_449_residues
PPPRFATPSEVPGLAARRIAADVIDGVLHKHRTLDDQLDGPGAHPGLKQLADRDRALMRRLVATTLRRLGTLGNVLSRLL
DRGIPTDAPRAQSALLIGAAQILWMDVPDHAAVDLSVRLVQADRRAAKYAGLVNAVLRRCAREGAALLDDAKGQPLDIAP
WLLKRWTDHYGEATARDIAAALGQEPSLDLTVKSDPAQWASRLHGEVLPTGTVRTLLHGSVTVLPGFAEGQWWVQDAAAA
LPARLFGDVAGKSMADLCAAPGGKTAQLAHGGARVTAVDRSPARMSRLKDNMTRLQLPAETVVTDAAEWQPADAGGFDGI
LLDAPCTSTGTIRRHPDVGWLRNEADIAALSGTQVRLLTRAAGLLKPGGTLVYCTCSLEPEEGEQVVDHVLAAGTELRRV
PIDPAEVAGLAELVTPMGDLRTLPCHLPHADPRLGGLDGFYAARLERRP

Specific function: Specifically methylates the cytosine at position 967 (m5C967) of 16S rRNA [H]

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Homo sapiens, GI76150625, Length=286, Percent_Identity=30.7692307692308, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI76150623, Length=286, Percent_Identity=30.7692307692308, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI32698918, Length=199, Percent_Identity=31.1557788944724, Blast_Score=86, Evalue=9e-17,
Organism=Homo sapiens, GI40316918, Length=212, Percent_Identity=32.5471698113208, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI39995082, Length=176, Percent_Identity=34.6590909090909, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI301336155, Length=176, Percent_Identity=34.6590909090909, Blast_Score=79, Evalue=1e-14,
Organism=Escherichia coli, GI2367212, Length=452, Percent_Identity=32.3008849557522, Blast_Score=173, Evalue=2e-44,
Organism=Escherichia coli, GI87081985, Length=257, Percent_Identity=31.1284046692607, Blast_Score=95, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI17536757, Length=242, Percent_Identity=30.1652892561983, Blast_Score=93, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI71998419, Length=228, Percent_Identity=30.2631578947368, Blast_Score=76, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI115534021, Length=228, Percent_Identity=30.2631578947368, Blast_Score=75, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI25143471, Length=172, Percent_Identity=33.1395348837209, Blast_Score=71, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17509941, Length=166, Percent_Identity=30.7228915662651, Blast_Score=69, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6324268, Length=240, Percent_Identity=32.0833333333333, Blast_Score=96, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6319447, Length=189, Percent_Identity=33.3333333333333, Blast_Score=82, Evalue=1e-16,
Organism=Drosophila melanogaster, GI22024126, Length=236, Percent_Identity=29.6610169491525, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI21355201, Length=179, Percent_Identity=34.0782122905028, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI21356579, Length=181, Percent_Identity=27.0718232044199, Blast_Score=72, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24668781, Length=183, Percent_Identity=31.1475409836066, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI21356513, Length=183, Percent_Identity=31.1475409836066, Blast_Score=69, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR018314
- InterPro:   IPR006027
- InterPro:   IPR004573 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu; PF01029 NusB [H]

EC number: =2.1.1.176 [H]

Molecular weight: Translated: 47961; Mature: 47830

Theoretical pI: Translated: 7.14; Mature: 7.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPPRFATPSEVPGLAARRIAADVIDGVLHKHRTLDDQLDGPGAHPGLKQLADRDRALMR
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCHHHHHHHHHHHHHHH
RLVATTLRRLGTLGNVLSRLLDRGIPTDAPRAQSALLIGAAQILWMDVPDHAAVDLSVRL
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHEEEEEEEEEECCCCCEEEHEEEE
VQADRRAAKYAGLVNAVLRRCAREGAALLDDAKGQPLDIAPWLLKRWTDHYGEATARDIA
EHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCHHHHHHHHHHHHHCCHHHHHHH
AALGQEPSLDLTVKSDPAQWASRLHGEVLPTGTVRTLLHGSVTVLPGFAEGQWWVQDAAA
HHHCCCCCEEEEECCCHHHHHHHHCCCCCCCHHHHHHHCCCEEEECCCCCCCHHHHHHHH
ALPARLFGDVAGKSMADLCAAPGGKTAQLAHGGARVTAVDRSPARMSRLKDNMTRLQLPA
HHHHHHHHHHHCCHHHHHHCCCCCCEEEEECCCEEEEEECCCHHHHHHHHHHHHHHCCCH
ETVVTDAAEWQPADAGGFDGILLDAPCTSTGTIRRHPDVGWLRNEADIAALSGTQVRLLT
HHHHHCCCCCCCCCCCCCCCEEEECCCCCCCCEEECCCCCCCCCCCCEEEECCCHHHHHH
RAAGLLKPGGTLVYCTCSLEPEEGEQVVDHVLAAGTELRRVPIDPAEVAGLAELVTPMGD
HHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHHCCCHHEECCCCHHHHHHHHHHHCCCHH
LRTLPCHLPHADPRLGGLDGFYAARLERRP
HCCCCCCCCCCCCCCCCCCHHHHHHHCCCC
>Mature Secondary Structure 
PPPRFATPSEVPGLAARRIAADVIDGVLHKHRTLDDQLDGPGAHPGLKQLADRDRALMR
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCHHHHHHHHHHHHHHH
RLVATTLRRLGTLGNVLSRLLDRGIPTDAPRAQSALLIGAAQILWMDVPDHAAVDLSVRL
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHEEEEEEEEEECCCCCEEEHEEEE
VQADRRAAKYAGLVNAVLRRCAREGAALLDDAKGQPLDIAPWLLKRWTDHYGEATARDIA
EHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCHHHHHHHHHHHHHCCHHHHHHH
AALGQEPSLDLTVKSDPAQWASRLHGEVLPTGTVRTLLHGSVTVLPGFAEGQWWVQDAAA
HHHCCCCCEEEEECCCHHHHHHHHCCCCCCCHHHHHHHCCCEEEECCCCCCCHHHHHHHH
ALPARLFGDVAGKSMADLCAAPGGKTAQLAHGGARVTAVDRSPARMSRLKDNMTRLQLPA
HHHHHHHHHHHCCHHHHHHCCCCCCEEEEECCCEEEEEECCCHHHHHHHHHHHHHHCCCH
ETVVTDAAEWQPADAGGFDGILLDAPCTSTGTIRRHPDVGWLRNEADIAALSGTQVRLLT
HHHHHCCCCCCCCCCCCCCCEEEECCCCCCCCEEECCCCCCCCCCCCEEEECCCHHHHHH
RAAGLLKPGGTLVYCTCSLEPEEGEQVVDHVLAAGTELRRVPIDPAEVAGLAELVTPMGD
HHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHHCCCHHEECCCCHHHHHHHHHHHCCCHH
LRTLPCHLPHADPRLGGLDGFYAARLERRP
HCCCCCCCCCCCCCCCCCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA