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The map label for this gene is gpsA

Identifier: 146337460

GI number: 146337460

Start: 317176

End: 318159

Strand: Direct

Name: gpsA

Synonym: BRADO0303

Alternate gene names: 146337460

Gene position: 317176-318159 (Clockwise)

Preceding gene: 146337459

Following gene: 146337461

Centisome position: 4.25

GC content: 71.85

Gene sequence:

>984_bases
GTGTCGCATCGCTTTCAAACGATCGCCGTCGTCGGCGCCGGTGCGTGGGGCACAGCGCTTGCGGCCGTGGCCGCGCGCGC
CGGCCGCAACGTGACGCTCTATGCCCGCGACGCCGATCACGCCGTTCGCATCGCGACAGCGCGTGAGAATCCCCGTCTGC
CCGGCATTCCGCTCGCCGACAGCATCACCGTCACTACCGACCTCGCCCGGGCCGCCCGCGCCGACGCCATCCTGATCGTC
GTTCCCGCGCAGCATCTGCGCGGTGCCGTCATGCATCTCGCGCCTCTGCTTGCGCCCGGCACGCCGCTTGTCGCCTGCGC
CAAGGGCATCGAGCACAGCACTCACAAATTCATGACGGAGGTGATCGCCGAGGCTGCGCCACGTGCAACGCCGGCGATCC
TGTCCGGCCCCAGCTTCGCCGACGACGTCGCGCGCGGACTGCCGACTGCGGTCACGCTCGCCGCGCCTGACGAGGCGCTT
GCCTCGGCGCTGGTGCAGGCGCTGGGCTCGTCGACCTTCCGGCCCTATCACTCGACCGACGTGCGCGGCGTCGAGATCGG
TGGTGCCGCCAAGAACGTGCTGGCGATCGCCGCCGGCATCGTCTCAGGGCGCGATCTCGGCGCCTCCGCGCTGGCGGCGC
TGACCACGCGCGGCTTTGCCGAACTGGTGCGGCTCGGCCGCGCCTATGGCGCCCGCAGCGAGACGTTGACCGGCCTGTCC
GGCCTCGGCGACCTGATTCTCACCTGCGCCGGTCCGCAATCGCGCAATTTCGCCGCCGGCCTCGCGCTCGGACGCGGCGA
GCCGCTGCCGGCCGGCAAGCTCGCCGAAGGCCAATACACCGCGCCGGTGCTGGTCGAACTCGCGGCGTCGCGCGGCGTCG
AGATGCCGGTGGCGCAGGCGGTGGCCGCGATCCTGTCCGGCACCGTGACGATCGACGCCGCGATCGAGGCGCTGATGTTG
CGGCCGTTCAAGGCGGAGGAGTGA

Upstream 100 bases:

>100_bases
CCCCACGCGTGGCGCCTCTCCGCGCGATCGGTGGGTGGGGCTGCCCCTCACCCCAACCCTCTCCCCGTAAGAACGGGCAG
AGGGAGCACTGAGGCCGCCC

Downstream 100 bases:

>100_bases
GGCGGACCGCTGGGCAAAAGCGCGATGTCTTCGCTCTAAACTGTTGACCCGGCGATTGCCGTCTTCTTTGATCCGCCCGC
CGAGCAAGCCCGTCTTCCGC

Product: NAD(P)H-dependent glycerol-3-phosphate dehydrogenase

Products: NA

Alternate protein names: NAD(P)H-dependent glycerol-3-phosphate dehydrogenase

Number of amino acids: Translated: 327; Mature: 326

Protein sequence:

>327_residues
MSHRFQTIAVVGAGAWGTALAAVAARAGRNVTLYARDADHAVRIATARENPRLPGIPLADSITVTTDLARAARADAILIV
VPAQHLRGAVMHLAPLLAPGTPLVACAKGIEHSTHKFMTEVIAEAAPRATPAILSGPSFADDVARGLPTAVTLAAPDEAL
ASALVQALGSSTFRPYHSTDVRGVEIGGAAKNVLAIAAGIVSGRDLGASALAALTTRGFAELVRLGRAYGARSETLTGLS
GLGDLILTCAGPQSRNFAAGLALGRGEPLPAGKLAEGQYTAPVLVELAASRGVEMPVAQAVAAILSGTVTIDAAIEALML
RPFKAEE

Sequences:

>Translated_327_residues
MSHRFQTIAVVGAGAWGTALAAVAARAGRNVTLYARDADHAVRIATARENPRLPGIPLADSITVTTDLARAARADAILIV
VPAQHLRGAVMHLAPLLAPGTPLVACAKGIEHSTHKFMTEVIAEAAPRATPAILSGPSFADDVARGLPTAVTLAAPDEAL
ASALVQALGSSTFRPYHSTDVRGVEIGGAAKNVLAIAAGIVSGRDLGASALAALTTRGFAELVRLGRAYGARSETLTGLS
GLGDLILTCAGPQSRNFAAGLALGRGEPLPAGKLAEGQYTAPVLVELAASRGVEMPVAQAVAAILSGTVTIDAAIEALML
RPFKAEE
>Mature_326_residues
SHRFQTIAVVGAGAWGTALAAVAARAGRNVTLYARDADHAVRIATARENPRLPGIPLADSITVTTDLARAARADAILIVV
PAQHLRGAVMHLAPLLAPGTPLVACAKGIEHSTHKFMTEVIAEAAPRATPAILSGPSFADDVARGLPTAVTLAAPDEALA
SALVQALGSSTFRPYHSTDVRGVEIGGAAKNVLAIAAGIVSGRDLGASALAALTTRGFAELVRLGRAYGARSETLTGLSG
LGDLILTCAGPQSRNFAAGLALGRGEPLPAGKLAEGQYTAPVLVELAASRGVEMPVAQAVAAILSGTVTIDAAIEALMLR
PFKAEE

Specific function: De novo phospholipid biosynthesis; glycerol-3 phosphate formation. [C]

COG id: COG0240

COG function: function code C; Glycerol-3-phosphate dehydrogenase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD-dependent glycerol-3-phosphate dehydrogenase family

Homologues:

Organism=Homo sapiens, GI33695088, Length=345, Percent_Identity=26.9565217391304, Blast_Score=98, Evalue=9e-21,
Organism=Homo sapiens, GI24307999, Length=345, Percent_Identity=23.4782608695652, Blast_Score=79, Evalue=5e-15,
Organism=Escherichia coli, GI1790037, Length=315, Percent_Identity=41.9047619047619, Blast_Score=218, Evalue=5e-58,
Organism=Caenorhabditis elegans, GI17507425, Length=336, Percent_Identity=27.0833333333333, Blast_Score=97, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI32564399, Length=349, Percent_Identity=25.7879656160458, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI32564403, Length=358, Percent_Identity=25.9776536312849, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI193210136, Length=358, Percent_Identity=25.9776536312849, Blast_Score=90, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6324513, Length=352, Percent_Identity=26.9886363636364, Blast_Score=96, Evalue=6e-21,
Organism=Saccharomyces cerevisiae, GI6320181, Length=345, Percent_Identity=27.536231884058, Blast_Score=88, Evalue=2e-18,
Organism=Drosophila melanogaster, GI17136204, Length=344, Percent_Identity=25, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI17136202, Length=344, Percent_Identity=25, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI17136200, Length=344, Percent_Identity=25, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI22026922, Length=344, Percent_Identity=23.8372093023256, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI281362270, Length=337, Percent_Identity=25.2225519287834, Blast_Score=78, Evalue=7e-15,
Organism=Drosophila melanogaster, GI45551945, Length=246, Percent_Identity=27.2357723577236, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24648969, Length=237, Percent_Identity=26.5822784810127, Blast_Score=65, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GPDA_BRASO (A4YK34)

Other databases:

- EMBL:   CU234118
- RefSeq:   YP_001202508.1
- ProteinModelPortal:   A4YK34
- SMR:   A4YK34
- STRING:   A4YK34
- GeneID:   5116706
- GenomeReviews:   CU234118_GR
- KEGG:   bra:BRADO0303
- eggNOG:   COG0240
- HOGENOM:   HBG586392
- OMA:   AKGIEHG
- ProtClustDB:   PRK00094
- BioCyc:   BSP376:BRADO0303-MONOMER
- HAMAP:   MF_00394
- InterPro:   IPR008927
- InterPro:   IPR013328
- InterPro:   IPR006168
- InterPro:   IPR006109
- InterPro:   IPR011128
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720
- Gene3D:   G3DSA:1.10.1040.10
- PANTHER:   PTHR11728
- PIRSF:   PIRSF000114
- PRINTS:   PR00077

Pfam domain/function: PF07479 NAD_Gly3P_dh_C; PF01210 NAD_Gly3P_dh_N; SSF48179 6DGDH_C_like

EC number: =1.1.1.94

Molecular weight: Translated: 33199; Mature: 33067

Theoretical pI: Translated: 8.26; Mature: 8.26

Prosite motif: PS00957 NAD_G3PDH

Important sites: ACT_SITE 191-191 BINDING 108-108 BINDING 108-108 BINDING 140-140 BINDING 255-255 BINDING 276-276

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHRFQTIAVVGAGAWGTALAAVAARAGRNVTLYARDADHAVRIATARENPRLPGIPLAD
CCCCEEEEEEEECCHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEECCCCCCCCCCCCCC
SITVTTDLARAARADAILIVVPAQHLRGAVMHLAPLLAPGTPLVACAKGIEHSTHKFMTE
CEEHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHCCCCCHHHHHHHCCHHHHHHHHH
VIAEAAPRATPAILSGPSFADDVARGLPTAVTLAAPDEALASALVQALGSSTFRPYHSTD
HHHHHCCCCCCHHCCCCCHHHHHHHCCCCEEEEECCHHHHHHHHHHHHCCCCCCCCCCCC
VRGVEIGGAAKNVLAIAAGIVSGRDLGASALAALTTRGFAELVRLGRAYGARSETLTGLS
CCEEEECCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
GLGDLILTCAGPQSRNFAAGLALGRGEPLPAGKLAEGQYTAPVLVELAASRGVEMPVAQA
HHCCEEEEECCCCCCCCHHEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHH
VAAILSGTVTIDAAIEALMLRPFKAEE
HHHHHHCCCHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SHRFQTIAVVGAGAWGTALAAVAARAGRNVTLYARDADHAVRIATARENPRLPGIPLAD
CCCEEEEEEEECCHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEECCCCCCCCCCCCCC
SITVTTDLARAARADAILIVVPAQHLRGAVMHLAPLLAPGTPLVACAKGIEHSTHKFMTE
CEEHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHCCCCCHHHHHHHCCHHHHHHHHH
VIAEAAPRATPAILSGPSFADDVARGLPTAVTLAAPDEALASALVQALGSSTFRPYHSTD
HHHHHCCCCCCHHCCCCCHHHHHHHCCCCEEEEECCHHHHHHHHHHHHCCCCCCCCCCCC
VRGVEIGGAAKNVLAIAAGIVSGRDLGASALAALTTRGFAELVRLGRAYGARSETLTGLS
CCEEEECCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
GLGDLILTCAGPQSRNFAAGLALGRGEPLPAGKLAEGQYTAPVLVELAASRGVEMPVAQA
HHCCEEEEECCCCCCCCHHEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHH
VAAILSGTVTIDAAIEALMLRPFKAEE
HHHHHHCCCHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA