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The map label for this gene is dapE [H]

Identifier: 146337436

GI number: 146337436

Start: 289439

End: 290851

Strand: Direct

Name: dapE [H]

Synonym: BRADO0276

Alternate gene names: 146337436

Gene position: 289439-290851 (Clockwise)

Preceding gene: 146337435

Following gene: 146337445

Centisome position: 3.88

GC content: 66.53

Gene sequence:

>1413_bases
GTGCTCGTCAACACGTTTCGCCAGGGATCCCGGCAGCTCGAGGTGGCAGCGCTGCCGAAGGCCAGCGTCGATCAAGCGGC
CGCCGCGCAGCGGCTCGCGGAGGCGGTTCGTTTTCGCACGGTCTCGAGCCCCGATGATCCCGAGCTGAACGCCGAGGCTT
TCGCGGCGCTCAGAGCGCATATCGAAGCAAGCTTTCCGGCCTTCCATACGGCCGCGGCTCAGGAGGTCGTGGCCGGGCAC
AGCCTGCTGTACACGTGGCGAGGCAGCGACACGTCGGCGAAGCCGATCGCACTTCTCGCCCATCAGGACGTGGTGCCGAT
CGCGCCAGGCACCGAGCCCGATTGGGCCGTGCCGCCCTTTGCCGGTGTCATCAAGGAGGGCTTCGTCTGGGGCCGCGGCG
CCTGGGACGACAAGGGCAATCTGTATGCGATGCTCGAGGCGGCCGAAGCGCTGATCAAGGCCGGCTTCAAGCCGAGGCGC
ACGATCTATTTCGCCTTCGGCCACGACGAGGAGGTCGGCGGCGTCAGAGGCGCCAAGGCCATGTCGGCTATCCTCGCGGC
TCGCAACGTTCGACTGGACTTCGTCATCGATGAGGGCCTCCTGATCTCGGAGGGCGGCATCAAGGGCCTCGACAAGCCTG
CCGCGCTGGTCGGTGTCGCCGAGAAGGGCTATGCGAGCCTGGTGCTGACCGCCAAGGCCACCCCCGGCCATTCCTCGATG
CCGCCGCGGGACACGGCGATCGGCATGATGAGCGCGGCACTGGCCAAGCTCGAGGCCAACCGGCTGCCGATGCGAATCGA
CGGCACGGTCGGCGAGATGTTCGCCGCGCTGGCGCCGGAAATGAGCGGTATCAACCGCGCCGTGCTGTCCAATCTCTGGC
TGACCAAGCCGCTGCTGTTCAGCGAGTTCGCCAAGAGCGGACCGGCCGAGGCGATGGTGCGCACGACGACCGCGCTGACC
ATCTTCAACGCCGGCGACAAGGACAATGTGCTCCCGGGCAACGCCTCAGCCACCGTGAATTTCCGACTGCTGCCAGGTGA
CACCGAGGCTGGGATCATCGATCACATCAGGCAGACCGTGGCCAACGACCGCATCTCGATCTCAGCCTTGCGCGGCAACC
GCGAGCCGCCGCTGGTCACCTCGACCGCCAGCCCGGCCTATCAGCTCGTCAACCGCACCATTCGCGAGATCTTCAATGAT
GCGGTGGTGGCGCCCGGCCTGATGATCGCCGGCACCGATTCGAGCCACTATGCGGGCATCGCCGACAGCATCTTCCGCTT
CTCGCCGCTGCGCGCGACGACGGAGGATCTCAAGCGCTTTCACGGCACCAATGAGCGGCTGTCGGTCGAAGGCTATGGCG
ACATGATCCGCTTCTATCGGCGGCTGCTGGAGACAGCCGCTGGTCCGGCCTAG

Upstream 100 bases:

>100_bases
GCCGTCTTGTTTGGGAGCTTCTGCGATGCGCCGGCTGCTCAGGATCGTTCGAGCCGTTGTCCTTCTCATCCTCGCGGTGG
GCGCGGCGCTGCTTGCGGTC

Downstream 100 bases:

>100_bases
ACCGAAGGCTTCGGCAACCCGGCGATGGCGCAGGCCGCGCGCAGCGTGTTCACCAGCAGGCAGGCCACCGTCATCTGGCC
GACGCCGCCCGGCACCGGCG

Product: hypothetical protein

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase [H]

Number of amino acids: Translated: 470; Mature: 470

Protein sequence:

>470_residues
MLVNTFRQGSRQLEVAALPKASVDQAAAAQRLAEAVRFRTVSSPDDPELNAEAFAALRAHIEASFPAFHTAAAQEVVAGH
SLLYTWRGSDTSAKPIALLAHQDVVPIAPGTEPDWAVPPFAGVIKEGFVWGRGAWDDKGNLYAMLEAAEALIKAGFKPRR
TIYFAFGHDEEVGGVRGAKAMSAILAARNVRLDFVIDEGLLISEGGIKGLDKPAALVGVAEKGYASLVLTAKATPGHSSM
PPRDTAIGMMSAALAKLEANRLPMRIDGTVGEMFAALAPEMSGINRAVLSNLWLTKPLLFSEFAKSGPAEAMVRTTTALT
IFNAGDKDNVLPGNASATVNFRLLPGDTEAGIIDHIRQTVANDRISISALRGNREPPLVTSTASPAYQLVNRTIREIFND
AVVAPGLMIAGTDSSHYAGIADSIFRFSPLRATTEDLKRFHGTNERLSVEGYGDMIRFYRRLLETAAGPA

Sequences:

>Translated_470_residues
MLVNTFRQGSRQLEVAALPKASVDQAAAAQRLAEAVRFRTVSSPDDPELNAEAFAALRAHIEASFPAFHTAAAQEVVAGH
SLLYTWRGSDTSAKPIALLAHQDVVPIAPGTEPDWAVPPFAGVIKEGFVWGRGAWDDKGNLYAMLEAAEALIKAGFKPRR
TIYFAFGHDEEVGGVRGAKAMSAILAARNVRLDFVIDEGLLISEGGIKGLDKPAALVGVAEKGYASLVLTAKATPGHSSM
PPRDTAIGMMSAALAKLEANRLPMRIDGTVGEMFAALAPEMSGINRAVLSNLWLTKPLLFSEFAKSGPAEAMVRTTTALT
IFNAGDKDNVLPGNASATVNFRLLPGDTEAGIIDHIRQTVANDRISISALRGNREPPLVTSTASPAYQLVNRTIREIFND
AVVAPGLMIAGTDSSHYAGIADSIFRFSPLRATTEDLKRFHGTNERLSVEGYGDMIRFYRRLLETAAGPA
>Mature_470_residues
MLVNTFRQGSRQLEVAALPKASVDQAAAAQRLAEAVRFRTVSSPDDPELNAEAFAALRAHIEASFPAFHTAAAQEVVAGH
SLLYTWRGSDTSAKPIALLAHQDVVPIAPGTEPDWAVPPFAGVIKEGFVWGRGAWDDKGNLYAMLEAAEALIKAGFKPRR
TIYFAFGHDEEVGGVRGAKAMSAILAARNVRLDFVIDEGLLISEGGIKGLDKPAALVGVAEKGYASLVLTAKATPGHSSM
PPRDTAIGMMSAALAKLEANRLPMRIDGTVGEMFAALAPEMSGINRAVLSNLWLTKPLLFSEFAKSGPAEAMVRTTTALT
IFNAGDKDNVLPGNASATVNFRLLPGDTEAGIIDHIRQTVANDRISISALRGNREPPLVTSTASPAYQLVNRTIREIFND
AVVAPGLMIAGTDSSHYAGIADSIFRFSPLRATTEDLKRFHGTNERLSVEGYGDMIRFYRRLLETAAGPA

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily [H]

Homologues:

Organism=Homo sapiens, GI269846968, Length=449, Percent_Identity=38.9755011135858, Blast_Score=296, Evalue=3e-80,
Organism=Homo sapiens, GI4501901, Length=395, Percent_Identity=25.0632911392405, Blast_Score=84, Evalue=3e-16,
Organism=Escherichia coli, GI1788816, Length=386, Percent_Identity=23.8341968911917, Blast_Score=79, Evalue=8e-16,
Organism=Saccharomyces cerevisiae, GI6322289, Length=493, Percent_Identity=30.2231237322515, Blast_Score=201, Evalue=1e-52,
Organism=Drosophila melanogaster, GI24649206, Length=391, Percent_Identity=25.0639386189258, Blast_Score=83, Evalue=5e-16,
Organism=Drosophila melanogaster, GI21356353, Length=392, Percent_Identity=23.469387755102, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24649212, Length=209, Percent_Identity=32.0574162679426, Blast_Score=79, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24649210, Length=417, Percent_Identity=24.4604316546763, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24649202, Length=392, Percent_Identity=26.530612244898, Blast_Score=75, Evalue=8e-14,
Organism=Drosophila melanogaster, GI24649204, Length=392, Percent_Identity=26.530612244898, Blast_Score=75, Evalue=8e-14,
Organism=Drosophila melanogaster, GI24649208, Length=390, Percent_Identity=25.1282051282051, Blast_Score=74, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001261
- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.18 [H]

Molecular weight: Translated: 50189; Mature: 50189

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: PS00758 ARGE_DAPE_CPG2_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLVNTFRQGSRQLEVAALPKASVDQAAAAQRLAEAVRFRTVSSPDDPELNAEAFAALRAH
CCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
IEASFPAFHTAAAQEVVAGHSLLYTWRGSDTSAKPIALLAHQDVVPIAPGTEPDWAVPPF
HHCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCEEEEECCCEEECCCCCCCCCCCCCH
AGVIKEGFVWGRGAWDDKGNLYAMLEAAEALIKAGFKPRRTIYFAFGHDEEVGGVRGAKA
HHHHHHCCEECCCCCCCCCCEEEHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCHHHH
MSAILAARNVRLDFVIDEGLLISEGGIKGLDKPAALVGVAEKGYASLVLTAKATPGHSSM
HHHHHHHCCCEEEEEECCCEEEECCCCCCCCCCHHEEEECCCCCEEEEEEEECCCCCCCC
PPRDTAIGMMSAALAKLEANRLPMRIDGTVGEMFAALAPEMSGINRAVLSNLWLTKPLLF
CCCHHHHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHCCCCCCCHHHHHHHHHHCCHHHH
SEFAKSGPAEAMVRTTTALTIFNAGDKDNVLPGNASATVNFRLLPGDTEAGIIDHIRQTV
HHHHCCCCHHHHEEEEEEEEEEECCCCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHH
ANDRISISALRGNREPPLVTSTASPAYQLVNRTIREIFNDAVVAPGLMIAGTDSSHYAGI
CCCCEEEEEECCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCH
ADSIFRFSPLRATTEDLKRFHGTNERLSVEGYGDMIRFYRRLLETAAGPA
HHHHHHCCCCCCCHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLVNTFRQGSRQLEVAALPKASVDQAAAAQRLAEAVRFRTVSSPDDPELNAEAFAALRAH
CCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
IEASFPAFHTAAAQEVVAGHSLLYTWRGSDTSAKPIALLAHQDVVPIAPGTEPDWAVPPF
HHCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCEEEEECCCEEECCCCCCCCCCCCCH
AGVIKEGFVWGRGAWDDKGNLYAMLEAAEALIKAGFKPRRTIYFAFGHDEEVGGVRGAKA
HHHHHHCCEECCCCCCCCCCEEEHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCHHHH
MSAILAARNVRLDFVIDEGLLISEGGIKGLDKPAALVGVAEKGYASLVLTAKATPGHSSM
HHHHHHHCCCEEEEEECCCEEEECCCCCCCCCCHHEEEECCCCCEEEEEEEECCCCCCCC
PPRDTAIGMMSAALAKLEANRLPMRIDGTVGEMFAALAPEMSGINRAVLSNLWLTKPLLF
CCCHHHHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHCCCCCCCHHHHHHHHHHCCHHHH
SEFAKSGPAEAMVRTTTALTIFNAGDKDNVLPGNASATVNFRLLPGDTEAGIIDHIRQTV
HHHHCCCCHHHHEEEEEEEEEEECCCCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHH
ANDRISISALRGNREPPLVTSTASPAYQLVNRTIREIFNDAVVAPGLMIAGTDSSHYAGI
CCCCEEEEEECCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCH
ADSIFRFSPLRATTEDLKRFHGTNERLSVEGYGDMIRFYRRLLETAAGPA
HHHHHHCCCCCCCHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14528314 [H]