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The map label for this gene is ppa [H]

Identifier: 146337435

GI number: 146337435

Start: 288708

End: 289244

Strand: Direct

Name: ppa [H]

Synonym: BRADO0275

Alternate gene names: 146337435

Gene position: 288708-289244 (Clockwise)

Preceding gene: 146337434

Following gene: 146337436

Centisome position: 3.87

GC content: 64.06

Gene sequence:

>537_bases
ATGCGCATCGACGCCATTCCGATCGGGAAAGATCCGCCGCGCGAGGTCAATGTCGTCATCGAGGTCCCGGTCGGCGGCGA
GCCGATCAAGTACGAGCTCGACAAGGAAGCTGGCACGCTGTTCGTCGACCGCTTCCTCTATACGGCGATGCGCTACCCCG
GCAATTACGGCTTCATTCCGCACACGCTGTCGAACGACGGCGACCCCTGCGACGTGCTGGTGGCCAACACCCGCGCGATC
GTGCCCGGCGCGGTTATGAGCGTGCGCCCGGTCGGCGTGCTGCTGATGGAGGACGAGGCCGGCGGCGATGAGAAGATCAT
CGCGGTGCCGTCCTCCAAGCTGACCCAGCGCTATGACAAGGTTCGCACCTATTCGGACCTGCCGGACATCACGCTGCAGC
AGATCCAGCACTTCTACGAGCACTACAAGGATCTCGAGCCCGGCAAATGGGTGAAGGTGCTGCGCTGGGGCAATGCCGAC
GACGCCCATCAGCTGATCGCCGAAGGCATCGCCCGCGCCAAGGCGGCGAAGAAGTAG

Upstream 100 bases:

>100_bases
GCGACTGCGAATGCAAGCGCTCATCTCGCGAGCGTCTGACCCGCAAGGGTCAAATCCTCAAGGGCCAAATCCTCAAACGT
TCAAGTTCCACGGAGCATCC

Downstream 100 bases:

>100_bases
GGGTCGTTCGCGCGCCGCGCCTCGCGGTTCGCGGCGATGGAGTTTCGATCCCGCCCCGATGCGGTTTCACGGCGCCGTAG
GTCTGATCTGCGGCGCCGTC

Product: inorganic pyrophosphatase

Products: NA

Alternate protein names: Pyrophosphate phospho-hydrolase; PPase [H]

Number of amino acids: Translated: 178; Mature: 178

Protein sequence:

>178_residues
MRIDAIPIGKDPPREVNVVIEVPVGGEPIKYELDKEAGTLFVDRFLYTAMRYPGNYGFIPHTLSNDGDPCDVLVANTRAI
VPGAVMSVRPVGVLLMEDEAGGDEKIIAVPSSKLTQRYDKVRTYSDLPDITLQQIQHFYEHYKDLEPGKWVKVLRWGNAD
DAHQLIAEGIARAKAAKK

Sequences:

>Translated_178_residues
MRIDAIPIGKDPPREVNVVIEVPVGGEPIKYELDKEAGTLFVDRFLYTAMRYPGNYGFIPHTLSNDGDPCDVLVANTRAI
VPGAVMSVRPVGVLLMEDEAGGDEKIIAVPSSKLTQRYDKVRTYSDLPDITLQQIQHFYEHYKDLEPGKWVKVLRWGNAD
DAHQLIAEGIARAKAAKK
>Mature_178_residues
MRIDAIPIGKDPPREVNVVIEVPVGGEPIKYELDKEAGTLFVDRFLYTAMRYPGNYGFIPHTLSNDGDPCDVLVANTRAI
VPGAVMSVRPVGVLLMEDEAGGDEKIIAVPSSKLTQRYDKVRTYSDLPDITLQQIQHFYEHYKDLEPGKWVKVLRWGNAD
DAHQLIAEGIARAKAAKK

Specific function: Unknown

COG id: COG0221

COG function: function code C; Inorganic pyrophosphatase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PPase family [H]

Homologues:

Organism=Escherichia coli, GI1790673, Length=174, Percent_Identity=56.8965517241379, Blast_Score=206, Evalue=1e-54,

Paralogues:

None

Copy number: 5480 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 200 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008162 [H]

Pfam domain/function: PF00719 Pyrophosphatase [H]

EC number: =3.6.1.1 [H]

Molecular weight: Translated: 19817; Mature: 19817

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIDAIPIGKDPPREVNVVIEVPVGGEPIKYELDKEAGTLFVDRFLYTAMRYPGNYGFIP
CEECCCCCCCCCCCCEEEEEEECCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCC
HTLSNDGDPCDVLVANTRAIVPGAVMSVRPVGVLLMEDEAGGDEKIIAVPSSKLTQRYDK
CCCCCCCCCEEEEEECCCEECCCCCEEECCEEEEEEECCCCCCCEEEECCCHHHHHHHHH
VRTYSDLPDITLQQIQHFYEHYKDLEPGKWVKVLRWGNADDAHQLIAEGIARAKAAKK
HHHHCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRIDAIPIGKDPPREVNVVIEVPVGGEPIKYELDKEAGTLFVDRFLYTAMRYPGNYGFIP
CEECCCCCCCCCCCCEEEEEEECCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCC
HTLSNDGDPCDVLVANTRAIVPGAVMSVRPVGVLLMEDEAGGDEKIIAVPSSKLTQRYDK
CCCCCCCCCEEEEEECCCEECCCCCEEECCEEEEEEECCCCCCCEEEECCCHHHHHHHHH
VRTYSDLPDITLQQIQHFYEHYKDLEPGKWVKVLRWGNADDAHQLIAEGIARAKAAKK
HHHHCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]