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The map label for this gene is hisH [H]

Identifier: 146337381

GI number: 146337381

Start: 226276

End: 226926

Strand: Direct

Name: hisH [H]

Synonym: BRADO0214

Alternate gene names: 146337381

Gene position: 226276-226926 (Clockwise)

Preceding gene: 146337380

Following gene: 146337382

Centisome position: 3.03

GC content: 64.52

Gene sequence:

>651_bases
ATGAGCGTCGCAATCATCGATTACGGCTCCGGCAACCTGCATTCCGCCGCGAAAGCGTTCGAGCGCGCCGCGCGCGGCAT
GGAAGAGCCGCAGAAGGTCGTCGTGACCCGCGATCCCGATACGGTGTATCGCGCCGACCGCATCGTGCTGCCGGGCGTCG
GCGCCTTCGCCGATTGCCGCCGCGGCCTCGATGCCGTCGACGGCATGCTCGAGGCGATGACCGAGGCCGTGCGCCACAAG
GCGCGCCCGTTCTTCGGCATCTGCGTCGGCATGCAGCTGATGGCGACGCGCGGCAAGGAGCATGTCACGACCGACGGCTT
CGACTGGGTCTCCGGCGACGTCGAGAAGATCACGCCCCGCGACGAGAGCCTGAAGATCCCGCACATGGGCTGGAATACGC
TCGACGTATTGCAGGAACATCCGGTGCTGGAGAAGCTGCCGCTCGGGCCGAAGGGTCTGCACGCCTATTTCGTGCACTCC
TATCATCTGAATGCCGTGAACGAGAACGATGTGCTGGCGCGCGCCGATTACGGCGGACCGATCACCGCCATCGTCGGCAA
GGACACCGCCATCGGCACGCAATTCCATCCCGAGAAGAGCCAGCGGTTCGGTCTCGCCTTGATTTCCAACTTCCTGAAAT
GGAAGCCGTGA

Upstream 100 bases:

>100_bases
GCGCCGCGCCGCCGACGCGTGACAATGGCAACGGCCAGCGGCCGCCGCCGCTACCCCATGCCAGCATCATTGGCTTGTTT
CCTGAACCCGGAGGTGGACG

Downstream 100 bases:

>100_bases
GCGCCGTCGTCCTGTTCGCCCCGGGGCGGGCAGGGCGCGCTCTTGAGAGTCACCAGATATGATCCTGTTTCCCGCCATCG
ATCTGAAGAACGGCCAATGC

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH [H]

Number of amino acids: Translated: 216; Mature: 215

Protein sequence:

>216_residues
MSVAIIDYGSGNLHSAAKAFERAARGMEEPQKVVVTRDPDTVYRADRIVLPGVGAFADCRRGLDAVDGMLEAMTEAVRHK
ARPFFGICVGMQLMATRGKEHVTTDGFDWVSGDVEKITPRDESLKIPHMGWNTLDVLQEHPVLEKLPLGPKGLHAYFVHS
YHLNAVNENDVLARADYGGPITAIVGKDTAIGTQFHPEKSQRFGLALISNFLKWKP

Sequences:

>Translated_216_residues
MSVAIIDYGSGNLHSAAKAFERAARGMEEPQKVVVTRDPDTVYRADRIVLPGVGAFADCRRGLDAVDGMLEAMTEAVRHK
ARPFFGICVGMQLMATRGKEHVTTDGFDWVSGDVEKITPRDESLKIPHMGWNTLDVLQEHPVLEKLPLGPKGLHAYFVHS
YHLNAVNENDVLARADYGGPITAIVGKDTAIGTQFHPEKSQRFGLALISNFLKWKP
>Mature_215_residues
SVAIIDYGSGNLHSAAKAFERAARGMEEPQKVVVTRDPDTVYRADRIVLPGVGAFADCRRGLDAVDGMLEAMTEAVRHKA
RPFFGICVGMQLMATRGKEHVTTDGFDWVSGDVEKITPRDESLKIPHMGWNTLDVLQEHPVLEKLPLGPKGLHAYFVHSY
HLNAVNENDVLARADYGGPITAIVGKDTAIGTQFHPEKSQRFGLALISNFLKWKP

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR [H]

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788334, Length=213, Percent_Identity=38.0281690140845, Blast_Score=122, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6319725, Length=223, Percent_Identity=32.7354260089686, Blast_Score=84, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 2.4.2.-

Molecular weight: Translated: 23793; Mature: 23661

Theoretical pI: Translated: 7.03; Mature: 7.03

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVAIIDYGSGNLHSAAKAFERAARGMEEPQKVVVTRDPDTVYRADRIVLPGVGAFADCR
CEEEEEECCCCCHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHCCEEEECCCCHHHHHH
RGLDAVDGMLEAMTEAVRHKARPFFGICVGMQLMATRGKEHVTTDGFDWVSGDVEKITPR
CCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHCCCC
DESLKIPHMGWNTLDVLQEHPVLEKLPLGPKGLHAYFVHSYHLNAVNENDVLARADYGGP
CCCEECCCCCCCHHHHHHHCCCHHHCCCCCCCCEEEEEEEEEECCCCCCCEEEECCCCCC
ITAIVGKDTAIGTQFHPEKSQRFGLALISNFLKWKP
EEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SVAIIDYGSGNLHSAAKAFERAARGMEEPQKVVVTRDPDTVYRADRIVLPGVGAFADCR
EEEEEECCCCCHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHCCEEEECCCCHHHHHH
RGLDAVDGMLEAMTEAVRHKARPFFGICVGMQLMATRGKEHVTTDGFDWVSGDVEKITPR
CCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHCCCC
DESLKIPHMGWNTLDVLQEHPVLEKLPLGPKGLHAYFVHSYHLNAVNENDVLARADYGGP
CCCEECCCCCCCHHHHHHHCCCHHHCCCCCCCCEEEEEEEEEECCCCCCCEEEECCCCCC
ITAIVGKDTAIGTQFHPEKSQRFGLALISNFLKWKP
EEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]