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The map label for this gene is pleD [H]

Identifier: 146337355

GI number: 146337355

Start: 199382

End: 200368

Strand: Reverse

Name: pleD [H]

Synonym: BRADO0188

Alternate gene names: 146337355

Gene position: 200368-199382 (Counterclockwise)

Preceding gene: 146337359

Following gene: 146337353

Centisome position: 2.69

GC content: 64.13

Gene sequence:

>987_bases
ATGGCCCAACAGCGCGTACCACCGACGCCTAACAACTTCCATCTGTGGTTCAAATACTCCCTCGGCTCCGCGTCCGACCT
CAAGCGCGCCGTCGACATCCTCGTCAGCAACAAGCGCAAGTTCGACAAGACCACCAACCAGGCGCTGTTCGACACCTATA
TCGGCCAGCAGGGCACGGAAGCGGCCGTCGTCCATAAAGTGTCGCAGCAGCTGCATGGCGTGATGGACTCCGCCAAGAGC
TATTTGACGGCCGCGATCGCCGACAACCGCACACACATGGCCGCGATCGACGGCGTCGCCGAGCGCAGCGAGCAGGGCGT
TGATCCGAAGATCCTGATCGAAAATCTCGTCAGCGAGCTGGCCCAGGCGACCGCGCGTGCCGCGAAGCTGGAAGCGAGCT
TTGCCGAGAAGTCGCGCGAGCTCGACACCATCCGCGACTCGCTCAACAAGTCGGAGCAGCGTGCGCGCACCGATACGCTC
ACCGGTCTGCCGAACCGCCGCGCGCTCGAGGAGTTCTTCCGTCATGCGCAGATCGAGGCGATGGAGCGCGACGAGCCGCT
CAGCATTCTCCTGATCGACGTCGACCACTTCAAGAAGTTCAACGACAATTATGGTCACGGCGTCGGCGACCAGGTGCTGC
GCCTCGTCGCCAAGGCGCTGCGCGAGCGCGTGCGCGAGAAGGATCTTCCCGCCCGCTATGGCGGCGAGGAGCTCATCGCG
GTGCTGCCGGAGACCGATCTCGAAGGCTGCACGGGAATGGCAGAGCGCATCCGGCGCACCATTGCCGACTGTCAGTTCAC
CCGCCGCTCGACCGGCGAGGTCCTGCCGCAGATCACGGTCTCGATCGGCGTTGGCCAGTTCCAGTTCGGCGAGTCGATGG
CCGATCTGATCGATCGCTGCGATCGCGCGCTGTATCTGGCCAAGAAGCTCGGCCGCAATCGCGTGGTGACCGAGCTCGAG
CTCGATCGCAAGCTGGCCGTTGGCTGA

Upstream 100 bases:

>100_bases
CCGCCCGCATCTGGGGTCACTCCACATCGTGTCCGGCGCTACGTTCGACGAGCTTGACTTCGAGTACGCGACGACTGTCG
CGGAAAAGGCCATGAAATCC

Downstream 100 bases:

>100_bases
CCGGCTTTCGCGGTGCCGGCTCAGCCGGCGCGTGCGACGTCACTGTCAGCCTCCAGGACATCGCCGAAACCATGCGCGAC
GACCGTGTTGCGACCACGAT

Product: GGDEF domain-containing protein

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 328; Mature: 327

Protein sequence:

>328_residues
MAQQRVPPTPNNFHLWFKYSLGSASDLKRAVDILVSNKRKFDKTTNQALFDTYIGQQGTEAAVVHKVSQQLHGVMDSAKS
YLTAAIADNRTHMAAIDGVAERSEQGVDPKILIENLVSELAQATARAAKLEASFAEKSRELDTIRDSLNKSEQRARTDTL
TGLPNRRALEEFFRHAQIEAMERDEPLSILLIDVDHFKKFNDNYGHGVGDQVLRLVAKALRERVREKDLPARYGGEELIA
VLPETDLEGCTGMAERIRRTIADCQFTRRSTGEVLPQITVSIGVGQFQFGESMADLIDRCDRALYLAKKLGRNRVVTELE
LDRKLAVG

Sequences:

>Translated_328_residues
MAQQRVPPTPNNFHLWFKYSLGSASDLKRAVDILVSNKRKFDKTTNQALFDTYIGQQGTEAAVVHKVSQQLHGVMDSAKS
YLTAAIADNRTHMAAIDGVAERSEQGVDPKILIENLVSELAQATARAAKLEASFAEKSRELDTIRDSLNKSEQRARTDTL
TGLPNRRALEEFFRHAQIEAMERDEPLSILLIDVDHFKKFNDNYGHGVGDQVLRLVAKALRERVREKDLPARYGGEELIA
VLPETDLEGCTGMAERIRRTIADCQFTRRSTGEVLPQITVSIGVGQFQFGESMADLIDRCDRALYLAKKLGRNRVVTELE
LDRKLAVG
>Mature_327_residues
AQQRVPPTPNNFHLWFKYSLGSASDLKRAVDILVSNKRKFDKTTNQALFDTYIGQQGTEAAVVHKVSQQLHGVMDSAKSY
LTAAIADNRTHMAAIDGVAERSEQGVDPKILIENLVSELAQATARAAKLEASFAEKSRELDTIRDSLNKSEQRARTDTLT
GLPNRRALEEFFRHAQIEAMERDEPLSILLIDVDHFKKFNDNYGHGVGDQVLRLVAKALRERVREKDLPARYGGEELIAV
LPETDLEGCTGMAERIRRTIADCQFTRRSTGEVLPQITVSIGVGQFQFGESMADLIDRCDRALYLAKKLGRNRVVTELEL
DRKLAVG

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI87081881, Length=165, Percent_Identity=41.8181818181818, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI145693134, Length=158, Percent_Identity=41.7721518987342, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI1787262, Length=181, Percent_Identity=37.5690607734807, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1787816, Length=165, Percent_Identity=38.7878787878788, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI1787802, Length=205, Percent_Identity=32.6829268292683, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI87082007, Length=170, Percent_Identity=37.0588235294118, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1786584, Length=160, Percent_Identity=31.25, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1787541, Length=160, Percent_Identity=35, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1788381, Length=172, Percent_Identity=30.8139534883721, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1788085, Length=199, Percent_Identity=28.1407035175879, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI87081977, Length=134, Percent_Identity=31.3432835820896, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI87081974, Length=160, Percent_Identity=25.625, Blast_Score=65, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 36695; Mature: 36564

Theoretical pI: Translated: 7.20; Mature: 7.20

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQQRVPPTPNNFHLWFKYSLGSASDLKRAVDILVSNKRKFDKTTNQALFDTYIGQQGTE
CCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCH
AAVVHKVSQQLHGVMDSAKSYLTAAIADNRTHMAAIDGVAERSEQGVDPKILIENLVSEL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
AQATARAAKLEASFAEKSRELDTIRDSLNKSEQRARTDTLTGLPNRRALEEFFRHAQIEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
MERDEPLSILLIDVDHFKKFNDNYGHGVGDQVLRLVAKALRERVREKDLPARYGGEELIA
HCCCCCCEEEEEEHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEE
VLPETDLEGCTGMAERIRRTIADCQFTRRSTGEVLPQITVSIGVGQFQFGESMADLIDRC
ECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHH
DRALYLAKKLGRNRVVTELELDRKLAVG
HHHHHHHHHHCCCCCCHHHHHCHHHCCC
>Mature Secondary Structure 
AQQRVPPTPNNFHLWFKYSLGSASDLKRAVDILVSNKRKFDKTTNQALFDTYIGQQGTE
CCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCH
AAVVHKVSQQLHGVMDSAKSYLTAAIADNRTHMAAIDGVAERSEQGVDPKILIENLVSEL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
AQATARAAKLEASFAEKSRELDTIRDSLNKSEQRARTDTLTGLPNRRALEEFFRHAQIEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
MERDEPLSILLIDVDHFKKFNDNYGHGVGDQVLRLVAKALRERVREKDLPARYGGEELIA
HCCCCCCEEEEEEHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEE
VLPETDLEGCTGMAERIRRTIADCQFTRRSTGEVLPQITVSIGVGQFQFGESMADLIDRC
ECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHH
DRALYLAKKLGRNRVVTELELDRKLAVG
HHHHHHHHHHCCCCCCHHHHHCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA