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The map label for this gene is grpE

Identifier: 146337339

GI number: 146337339

Start: 183959

End: 184579

Strand: Reverse

Name: grpE

Synonym: BRADO0171

Alternate gene names: 146337339

Gene position: 184579-183959 (Counterclockwise)

Preceding gene: 146337340

Following gene: 146337338

Centisome position: 2.48

GC content: 66.99

Gene sequence:

>621_bases
ATGACCGATCCCGACCTGCATCAGAACGATCCGGAAAACCCGGCGCAGGCCAGCGAACCCGTCGTCTCGAAGCCCTATAT
CATGCCCGACGACCCCGAGACCGGCTCGGCTGAGGCCTATGCGAAGGAAGCGGCCGAGGCGCGTGACAAGATGCTGCGCA
CGCTGGCGGAGATGGAGAACCTGCGCAAGCGGACCGCGCGCGAGGTCGCCGATGCGCGGATGTACGGCATCACCGGTTTC
GCCCGCGACGTGCTCGACATCGCCGACAATCTGCAGCGCGCGCTCGATGCCGTGCCGGCCGAGACTCGCGCCAATGCCGA
TGCGGGCCTGAAGTCGCTGATCGAGGGCGTCGAGCTCACCGAGCGCTCGCTGCTCAACACGCTGGAGAAGAACGGCGTCA
AGAAGTTTGATCCGACGGGACAGAAGTTCGATCCCAACTTCCAGCAGGCGATGTATGAGGTCCCCGATCCGTCGGTGCCA
TCCGGCACCGTGGTGCAGGTCGTGCAGGCCGGCTTCATGATCGGCGAGCGCGTGCTGCGCCCGGCGTTGGTCGGCGTCTC
CAAGGGCGGCGCCAAGGCGGCGCCCGCCGCGAGCAACGACCAGTCGAACAGCGCGGCCTGA

Upstream 100 bases:

>100_bases
CGCCGGCGCGATCGTCAGGTCGTGCTTGATTTTCGTGGTTCAAAGCACGATATCCCGCCCGACATTCCCAGAACCCGCCG
AACTTGCGAGAAGTCTGTCC

Downstream 100 bases:

>100_bases
GGCCGCTGGCTCCATCAGTTCATGCGACACGGGGCGCCCATCCAGTTCGGATGCGGCGCCCCAAGCGTTTTTGAACCTGG
GCGTGTCGTCTCCCAGGGAC

Product: heat shock protein GrpE

Products: NA

Alternate protein names: HSP-70 cofactor

Number of amino acids: Translated: 206; Mature: 205

Protein sequence:

>206_residues
MTDPDLHQNDPENPAQASEPVVSKPYIMPDDPETGSAEAYAKEAAEARDKMLRTLAEMENLRKRTAREVADARMYGITGF
ARDVLDIADNLQRALDAVPAETRANADAGLKSLIEGVELTERSLLNTLEKNGVKKFDPTGQKFDPNFQQAMYEVPDPSVP
SGTVVQVVQAGFMIGERVLRPALVGVSKGGAKAAPAASNDQSNSAA

Sequences:

>Translated_206_residues
MTDPDLHQNDPENPAQASEPVVSKPYIMPDDPETGSAEAYAKEAAEARDKMLRTLAEMENLRKRTAREVADARMYGITGF
ARDVLDIADNLQRALDAVPAETRANADAGLKSLIEGVELTERSLLNTLEKNGVKKFDPTGQKFDPNFQQAMYEVPDPSVP
SGTVVQVVQAGFMIGERVLRPALVGVSKGGAKAAPAASNDQSNSAA
>Mature_205_residues
TDPDLHQNDPENPAQASEPVVSKPYIMPDDPETGSAEAYAKEAAEARDKMLRTLAEMENLRKRTAREVADARMYGITGFA
RDVLDIADNLQRALDAVPAETRANADAGLKSLIEGVELTERSLLNTLEKNGVKKFDPTGQKFDPNFQQAMYEVPDPSVPS
GTVVQVVQAGFMIGERVLRPALVGVSKGGAKAAPAASNDQSNSAA

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family

Homologues:

Organism=Homo sapiens, GI24308295, Length=143, Percent_Identity=40.5594405594406, Blast_Score=117, Evalue=9e-27,
Organism=Homo sapiens, GI40255109, Length=148, Percent_Identity=32.4324324324324, Blast_Score=87, Evalue=8e-18,
Organism=Escherichia coli, GI1788967, Length=183, Percent_Identity=34.9726775956284, Blast_Score=103, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI17552458, Length=146, Percent_Identity=36.3013698630137, Blast_Score=108, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6324806, Length=151, Percent_Identity=39.7350993377483, Blast_Score=123, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24653432, Length=151, Percent_Identity=44.3708609271523, Blast_Score=136, Evalue=1e-32,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GRPE_BRASO (A4YJR1)

Other databases:

- EMBL:   CU234118
- RefSeq:   YP_001202387.1
- ProteinModelPortal:   A4YJR1
- SMR:   A4YJR1
- STRING:   A4YJR1
- GeneID:   5116614
- GenomeReviews:   CU234118_GR
- KEGG:   bra:BRADO0171
- eggNOG:   COG0576
- HOGENOM:   HBG732630
- OMA:   NVRRRME
- ProtClustDB:   PRK14141
- BioCyc:   BSP376:BRADO0171-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01151
- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012
- Gene3D:   G3DSA:3.90.20.20
- Gene3D:   G3DSA:2.30.22.10
- PANTHER:   PTHR21237
- PRINTS:   PR00773

Pfam domain/function: PF01025 GrpE; SSF51064 GrpE_head

EC number: NA

Molecular weight: Translated: 22097; Mature: 21966

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDPDLHQNDPENPAQASEPVVSKPYIMPDDPETGSAEAYAKEAAEARDKMLRTLAEMEN
CCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LRKRTAREVADARMYGITGFARDVLDIADNLQRALDAVPAETRANADAGLKSLIEGVELT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCHHHHHHHHHHHHHHH
ERSLLNTLEKNGVKKFDPTGQKFDPNFQQAMYEVPDPSVPSGTVVQVVQAGFMIGERVLR
HHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHH
PALVGVSKGGAKAAPAASNDQSNSAA
HHHHHCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TDPDLHQNDPENPAQASEPVVSKPYIMPDDPETGSAEAYAKEAAEARDKMLRTLAEMEN
CCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LRKRTAREVADARMYGITGFARDVLDIADNLQRALDAVPAETRANADAGLKSLIEGVELT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCHHHHHHHHHHHHHHH
ERSLLNTLEKNGVKKFDPTGQKFDPNFQQAMYEVPDPSVPSGTVVQVVQAGFMIGERVLR
HHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHH
PALVGVSKGGAKAAPAASNDQSNSAA
HHHHHCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA