BacMap

Definition	Bradyrhizobium sp. ORS278 chromosome, complete genome.
Accession	NC_009445
Length	7,456,587

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The map label for this gene is dnaJ [H]

Identifier: 146337332

GI number: 146337332

Start: 174885

End: 176015

Strand: Reverse

Name: dnaJ [H]

Synonym: BRADO0163

Alternate gene names: 146337332

Gene position: 176015-174885 (Counterclockwise)

Preceding gene: 146337333

Following gene: 146337331

Centisome position: 2.36

GC content: 68.26

Gene sequence:

>1131_bases
ATGTCCACCAAGCGCTGCTACTACGAGACCCTGGAAGTCGAGCGCGACGCCGACGAGACCAAGCTGAAGTCGGCCTTCCG
CAAGCTCGCCATGAAATGGCATCCGGACAAGAATCCGGGCGATGCCTCCAGCGAGGTCAAGTTCAAGGAGATCAACGAGG
CCTATGAGGTGCTGCGCGACGCCGACAAGCGCGCCGCCTATGACCGCTATGGCCATGCCGCGTTTGAGCAGGGCGCCGGC
GGTGGCCCGCACGGCTTCGGTGCCGGCTTCGCGTCGTCTTTCTCCGACATTTTCGAAGACCTGTTCGGCATGGCCGGGCA
GCGCCGCGGCGGCACCGGCCGCGAGCGCGGCGCCGACCTGCGCTACAACATGGAAATCACGCTGGAGGAGGCCTTCCTCG
GCAAGACCGCGCAGATCGAGATCCCGGTCTCGGTGACCTGCGAGCCCTGTTCCGGCACCGGCGCCAAGGCCGGCACCAAG
CCGAAGACCTGCGCCATGTGCGGCGGCGCCGGCCGCGTCCGCCAGGCGCAAGGCTTCTTCACGCTGGAGCGCACCTGTCC
CGGCTGCCATGGCCGCGGCCAGATGATCGAGGATCCCTGCCCGTCCTGCTCCGGCTCCGGCCGGGTGACGCGCGACCGCA
CGCTGTCGGTGAACATTCCGCAGGGCGTCGAGGACGGCACCCGCATCCGTCTCGCCGGCGAGGGCGAGGCCGGCCTGCGC
GGCGGTCCGCCGGGCGACCTCTACATCTTCCTGTCGCTCACCCCGCACGAGTTCTTTCAGCGCGACGGCGCCGACCTGCA
TTGCCGGGTGCCGATCTCGATGGTCACCGCGGCGCTCGGCGGCGAGTTCGAGGTGCCGACGATCGACAACGGCAAGACCA
AGGTCAAGATTCCCGCCGGTGCGCAATCCGGCCGCCGCTTCCGCATTGCCGCCAAGGGCATGCCGGTGCTGCGCTCGCGC
CAGACCGGCGACATGTACGTCCAGATCGTGGTCGAGACGCCGCAGAACCTCACCAAGAAGCAGCAGGAGCTCCTGGCGGA
GTTCGAGAAGCTGTCGTCCGGGGCGACCCAGCCGGAGGCCGCCGGCTTCTTCACCAAAGTGAAGGACTTCTTCGGCAAGG
CCGCGAGCTGA

Upstream 100 bases:

>100_bases
CGCTGTCAGCGTCTCGTTGGATGGGGGTCATTTTCATTGAACCGGCGCGCGGCAAGCGCGCGAGCCGTTGAGGTCTGACG
ATGTTCGGGCGGGTTGATTG

Downstream 100 bases:

>100_bases
GCGGTGCGCCCGCTCGGCAGTCATGCCGAGCGTCGTGCGATTGCTGCGATTTTGCTGCCCTGCACATGCAAAAAATTATC
AAATTCAACGGTATGAGCTC

Product: chaperone protein DnaJ

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 376; Mature: 375

Protein sequence:

>376_residues
MSTKRCYYETLEVERDADETKLKSAFRKLAMKWHPDKNPGDASSEVKFKEINEAYEVLRDADKRAAYDRYGHAAFEQGAG
GGPHGFGAGFASSFSDIFEDLFGMAGQRRGGTGRERGADLRYNMEITLEEAFLGKTAQIEIPVSVTCEPCSGTGAKAGTK
PKTCAMCGGAGRVRQAQGFFTLERTCPGCHGRGQMIEDPCPSCSGSGRVTRDRTLSVNIPQGVEDGTRIRLAGEGEAGLR
GGPPGDLYIFLSLTPHEFFQRDGADLHCRVPISMVTAALGGEFEVPTIDNGKTKVKIPAGAQSGRRFRIAAKGMPVLRSR
QTGDMYVQIVVETPQNLTKKQQELLAEFEKLSSGATQPEAAGFFTKVKDFFGKAAS

Sequences:

>Translated_376_residues
MSTKRCYYETLEVERDADETKLKSAFRKLAMKWHPDKNPGDASSEVKFKEINEAYEVLRDADKRAAYDRYGHAAFEQGAG
GGPHGFGAGFASSFSDIFEDLFGMAGQRRGGTGRERGADLRYNMEITLEEAFLGKTAQIEIPVSVTCEPCSGTGAKAGTK
PKTCAMCGGAGRVRQAQGFFTLERTCPGCHGRGQMIEDPCPSCSGSGRVTRDRTLSVNIPQGVEDGTRIRLAGEGEAGLR
GGPPGDLYIFLSLTPHEFFQRDGADLHCRVPISMVTAALGGEFEVPTIDNGKTKVKIPAGAQSGRRFRIAAKGMPVLRSR
QTGDMYVQIVVETPQNLTKKQQELLAEFEKLSSGATQPEAAGFFTKVKDFFGKAAS
>Mature_375_residues
STKRCYYETLEVERDADETKLKSAFRKLAMKWHPDKNPGDASSEVKFKEINEAYEVLRDADKRAAYDRYGHAAFEQGAGG
GPHGFGAGFASSFSDIFEDLFGMAGQRRGGTGRERGADLRYNMEITLEEAFLGKTAQIEIPVSVTCEPCSGTGAKAGTKP
KTCAMCGGAGRVRQAQGFFTLERTCPGCHGRGQMIEDPCPSCSGSGRVTRDRTLSVNIPQGVEDGTRIRLAGEGEAGLRG
GPPGDLYIFLSLTPHEFFQRDGADLHCRVPISMVTAALGGEFEVPTIDNGKTKVKIPAGAQSGRRFRIAAKGMPVLRSRQ
TGDMYVQIVVETPQNLTKKQQELLAEFEKLSSGATQPEAAGFFTKVKDFFGKAAS

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins and by disaggregating proteins, also in an autonomous, dnaK-independent fashion. Unfolded proteins bind initially to dnaJ; upon

COG id: COG0484

COG function: function code O; DnaJ-class molecular chaperone with C-terminal Zn finger domain

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 J domain [H]

Homologues:

Organism=Homo sapiens, GI205360838, Length=347, Percent_Identity=34.5821325648415, Blast_Score=211, Evalue=9e-55,
Organism=Homo sapiens, GI205360840, Length=347, Percent_Identity=34.5821325648415, Blast_Score=211, Evalue=1e-54,
Organism=Homo sapiens, GI5031741, Length=360, Percent_Identity=35.8333333333333, Blast_Score=175, Evalue=6e-44,
Organism=Homo sapiens, GI4504511, Length=349, Percent_Identity=34.6704871060172, Blast_Score=169, Evalue=3e-42,
Organism=Homo sapiens, GI194328760, Length=343, Percent_Identity=33.8192419825073, Blast_Score=164, Evalue=9e-41,
Organism=Homo sapiens, GI194328758, Length=343, Percent_Identity=33.8192419825073, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI7706495, Length=368, Percent_Identity=31.25, Blast_Score=145, Evalue=8e-35,
Organism=Homo sapiens, GI194328762, Length=312, Percent_Identity=31.7307692307692, Blast_Score=132, Evalue=4e-31,
Organism=Homo sapiens, GI6631085, Length=361, Percent_Identity=28.5318559556787, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI5453690, Length=373, Percent_Identity=26.2734584450402, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI9558755, Length=114, Percent_Identity=45.6140350877193, Blast_Score=97, Evalue=4e-20,
Organism=Homo sapiens, GI47777312, Length=103, Percent_Identity=50.4854368932039, Blast_Score=93, Evalue=4e-19,
Organism=Homo sapiens, GI39204547, Length=369, Percent_Identity=22.7642276422764, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI27151736, Length=107, Percent_Identity=44.8598130841121, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI88501736, Length=107, Percent_Identity=44.8598130841121, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI17388799, Length=103, Percent_Identity=49.5145631067961, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI4885495, Length=103, Percent_Identity=49.5145631067961, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI23503241, Length=103, Percent_Identity=43.6893203883495, Blast_Score=82, Evalue=8e-16,
Organism=Homo sapiens, GI21553335, Length=102, Percent_Identity=43.1372549019608, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI24308127, Length=79, Percent_Identity=44.3037974683544, Blast_Score=76, Evalue=5e-14,
Organism=Homo sapiens, GI201862518, Length=123, Percent_Identity=39.0243902439024, Blast_Score=76, Evalue=6e-14,
Organism=Homo sapiens, GI194306642, Length=105, Percent_Identity=43.8095238095238, Blast_Score=76, Evalue=6e-14,
Organism=Homo sapiens, GI194306640, Length=105, Percent_Identity=43.8095238095238, Blast_Score=76, Evalue=6e-14,
Organism=Homo sapiens, GI56549115, Length=123, Percent_Identity=39.0243902439024, Blast_Score=76, Evalue=6e-14,
Organism=Homo sapiens, GI201862587, Length=123, Percent_Identity=39.0243902439024, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI68077168, Length=78, Percent_Identity=50, Blast_Score=75, Evalue=8e-14,
Organism=Homo sapiens, GI68077166, Length=78, Percent_Identity=50, Blast_Score=75, Evalue=9e-14,
Organism=Homo sapiens, GI29126218, Length=70, Percent_Identity=47.1428571428571, Blast_Score=73, Evalue=5e-13,
Organism=Homo sapiens, GI45504382, Length=64, Percent_Identity=48.4375, Blast_Score=72, Evalue=8e-13,
Organism=Homo sapiens, GI22749377, Length=102, Percent_Identity=43.1372549019608, Blast_Score=70, Evalue=4e-12,
Organism=Homo sapiens, GI56687498, Length=66, Percent_Identity=48.4848484848485, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI72534740, Length=110, Percent_Identity=39.0909090909091, Blast_Score=67, Evalue=3e-11,
Organism=Escherichia coli, GI1786197, Length=376, Percent_Identity=52.1276595744681, Blast_Score=380, Evalue=1e-107,
Organism=Escherichia coli, GI1787235, Length=332, Percent_Identity=32.2289156626506, Blast_Score=153, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI17553098, Length=368, Percent_Identity=34.2391304347826, Blast_Score=189, Evalue=3e-48,
Organism=Caenorhabditis elegans, GI17553096, Length=368, Percent_Identity=34.2391304347826, Blast_Score=188, Evalue=3e-48,
Organism=Caenorhabditis elegans, GI17507263, Length=352, Percent_Identity=32.6704545454545, Blast_Score=144, Evalue=6e-35,
Organism=Caenorhabditis elegans, GI212645553, Length=359, Percent_Identity=32.3119777158774, Blast_Score=124, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI17563890, Length=227, Percent_Identity=29.0748898678414, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI115532378, Length=94, Percent_Identity=43.6170212765958, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17554874, Length=99, Percent_Identity=42.4242424242424, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17554644, Length=65, Percent_Identity=50.7692307692308, Blast_Score=75, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI86563276, Length=103, Percent_Identity=42.7184466019417, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17534355, Length=73, Percent_Identity=43.8356164383562, Blast_Score=71, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17538236, Length=110, Percent_Identity=37.2727272727273, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6324265, Length=352, Percent_Identity=34.0909090909091, Blast_Score=182, Evalue=6e-47,
Organism=Saccharomyces cerevisiae, GI6323119, Length=393, Percent_Identity=29.5165394402036, Blast_Score=139, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6321094, Length=403, Percent_Identity=32.258064516129, Blast_Score=138, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6323870, Length=358, Percent_Identity=31.0055865921788, Blast_Score=135, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6324252, Length=445, Percent_Identity=26.9662921348315, Blast_Score=107, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6323813, Length=133, Percent_Identity=37.593984962406, Blast_Score=75, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6322194, Length=92, Percent_Identity=41.304347826087, Blast_Score=70, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6324321, Length=67, Percent_Identity=50.7462686567164, Blast_Score=68, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6324103, Length=68, Percent_Identity=50, Blast_Score=65, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6320888, Length=99, Percent_Identity=38.3838383838384, Blast_Score=64, Evalue=3e-11,
Organism=Drosophila melanogaster, GI45552813, Length=370, Percent_Identity=30, Blast_Score=166, Evalue=4e-41,
Organism=Drosophila melanogaster, GI45549272, Length=370, Percent_Identity=30, Blast_Score=165, Evalue=4e-41,
Organism=Drosophila melanogaster, GI45552811, Length=370, Percent_Identity=30, Blast_Score=164, Evalue=7e-41,
Organism=Drosophila melanogaster, GI24646562, Length=348, Percent_Identity=35.3448275862069, Blast_Score=164, Evalue=8e-41,
Organism=Drosophila melanogaster, GI24646560, Length=348, Percent_Identity=35.3448275862069, Blast_Score=164, Evalue=8e-41,
Organism=Drosophila melanogaster, GI24646558, Length=348, Percent_Identity=35.3448275862069, Blast_Score=164, Evalue=8e-41,
Organism=Drosophila melanogaster, GI24646556, Length=348, Percent_Identity=35.3448275862069, Blast_Score=164, Evalue=8e-41,
Organism=Drosophila melanogaster, GI21357547, Length=348, Percent_Identity=35.3448275862069, Blast_Score=164, Evalue=8e-41,
Organism=Drosophila melanogaster, GI20129487, Length=338, Percent_Identity=34.0236686390533, Blast_Score=157, Evalue=1e-38,
Organism=Drosophila melanogaster, GI24584014, Length=338, Percent_Identity=34.0236686390533, Blast_Score=157, Evalue=1e-38,
Organism=Drosophila melanogaster, GI19920464, Length=351, Percent_Identity=32.1937321937322, Blast_Score=142, Evalue=3e-34,
Organism=Drosophila melanogaster, GI85726398, Length=363, Percent_Identity=27.5482093663912, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI28574949, Length=365, Percent_Identity=25.4794520547945, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI18858081, Length=355, Percent_Identity=27.0422535211268, Blast_Score=99, Evalue=6e-21,
Organism=Drosophila melanogaster, GI24762799, Length=133, Percent_Identity=35.3383458646617, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24654066, Length=66, Percent_Identity=53.030303030303, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24654070, Length=66, Percent_Identity=53.030303030303, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24654068, Length=66, Percent_Identity=53.030303030303, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24654072, Length=66, Percent_Identity=53.030303030303, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI161077138, Length=63, Percent_Identity=53.968253968254, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24658562, Length=67, Percent_Identity=50.7462686567164, Blast_Score=72, Evalue=9e-13,
Organism=Drosophila melanogaster, GI24658555, Length=67, Percent_Identity=50.7462686567164, Blast_Score=72, Evalue=9e-13,
Organism=Drosophila melanogaster, GI17737735, Length=64, Percent_Identity=46.875, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI28574723, Length=64, Percent_Identity=46.875, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24668523, Length=64, Percent_Identity=46.875, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012724
- InterPro:   IPR002939
- InterPro:   IPR001623
- InterPro:   IPR018253
- InterPro:   IPR008971
- InterPro:   IPR015609
- InterPro:   IPR003095
- InterPro:   IPR001305 [H]

Pfam domain/function: PF00226 DnaJ; PF01556 DnaJ_C; PF00684 DnaJ_CXXCXGXG [H]

EC number: NA

Molecular weight: Translated: 40677; Mature: 40546

Theoretical pI: Translated: 8.09; Mature: 8.09

Prosite motif: PS00636 DNAJ_1 ; PS50076 DNAJ_2 ; PS00637 DNAJ_CXXCXGXG

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTKRCYYETLEVERDADETKLKSAFRKLAMKWHPDKNPGDASSEVKFKEINEAYEVLRD
CCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHEECCCCCCCCCCCCCHHHHHHHHHHHHHH
ADKRAAYDRYGHAAFEQGAGGGPHGFGAGFASSFSDIFEDLFGMAGQRRGGTGRERGADL
HHHHHHHHHHCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCE
RYNMEITLEEAFLGKTAQIEIPVSVTCEPCSGTGAKAGTKPKTCAMCGGAGRVRQAQGFF
EEEEEEEEEHHHCCCCEEEEEEEEEEECCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCE
TLERTCPGCHGRGQMIEDPCPSCSGSGRVTRDRTLSVNIPQGVEDGTRIRLAGEGEAGLR
EEECCCCCCCCCCCCCCCCCCCCCCCCCEECCCEEEEECCCCCCCCCEEEEECCCCCCCC
GGPPGDLYIFLSLTPHEFFQRDGADLHCRVPISMVTAALGGEFEVPTIDNGKTKVKIPAG
CCCCCCEEEEEEECHHHHHHCCCCCEEEECCHHHHHHHHCCCEECCCCCCCCEEEEECCC
AQSGRRFRIAAKGMPVLRSRQTGDMYVQIVVETPQNLTKKQQELLAEFEKLSSGATQPEA
CCCCCEEEEEECCCCHHHCCCCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCHH
AGFFTKVKDFFGKAAS
HHHHHHHHHHHCCCCC
>Mature Secondary Structure 
STKRCYYETLEVERDADETKLKSAFRKLAMKWHPDKNPGDASSEVKFKEINEAYEVLRD
CCCHHHHHHHHCCCCCHHHHHHHHHHHHHHEECCCCCCCCCCCCCHHHHHHHHHHHHHH
ADKRAAYDRYGHAAFEQGAGGGPHGFGAGFASSFSDIFEDLFGMAGQRRGGTGRERGADL
HHHHHHHHHHCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCE
RYNMEITLEEAFLGKTAQIEIPVSVTCEPCSGTGAKAGTKPKTCAMCGGAGRVRQAQGFF
EEEEEEEEEHHHCCCCEEEEEEEEEEECCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCE
TLERTCPGCHGRGQMIEDPCPSCSGSGRVTRDRTLSVNIPQGVEDGTRIRLAGEGEAGLR
EEECCCCCCCCCCCCCCCCCCCCCCCCCEECCCEEEEECCCCCCCCCEEEEECCCCCCCC
GGPPGDLYIFLSLTPHEFFQRDGADLHCRVPISMVTAALGGEFEVPTIDNGKTKVKIPAG
CCCCCCEEEEEEECHHHHHHCCCCCEEEECCHHHHHHHHCCCEECCCCCCCCEEEEECCC
AQSGRRFRIAAKGMPVLRSRQTGDMYVQIVVETPQNLTKKQQELLAEFEKLSSGATQPEA
CCCCCEEEEEECCCCHHHCCCCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCHH
AGFFTKVKDFFGKAAS
HHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA