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The map label for this gene is rpoN [H]

Identifier: 146337286

GI number: 146337286

Start: 126924

End: 128564

Strand: Reverse

Name: rpoN [H]

Synonym: BRADO0113

Alternate gene names: 146337286

Gene position: 128564-126924 (Counterclockwise)

Preceding gene: 146337287

Following gene: 146337285

Centisome position: 1.72

GC content: 65.39

Gene sequence:

>1641_bases
ATGGCGTTGACGCAGAGACTAGAATTCCGCCAGTCACAGTCGCTGGTGATGACGCCGCAGCTGATGCAGGCGATCAAGCT
GCTGCAGCTGTCCAACCTCGACCTCTCCACGTTCGTCGAAGAAGAGCTCGAGCGCAATCCGCTGCTCGAGCGCGCGAACG
ATGGCCCGGAAGCGCCGGTCGCCGGCGAGCAGCAGATGGCCGAGCGCGGCGAGTTCTCCGAAGGCGGCGAAGCCAGCGGC
GATGATTTTGGCGATGGCGCGGGCAGCAGCGGCGGCGAGAGTTACGAGAGCGCGCCCGAGGACTGGATGAGCCGCGATCT
CGGCTCGCGCACGGAGATCGAACAGACGCTCGATACCGGGCTCGACAACGTGTTCTCCGAGGAGCCGGCGGAGGCCGCGG
CGCGCAATGCGCAGGACGCCGCGCCGACCACCTACACGGAATGGGGCGGCGGCGCCTCCGGCGACGAAGACTACAACCTC
GAAGCCTTCGTCGCGGCGGAAACCACGCTGTCGGATCATCTCGCCGAGCAGGCCGCGGTCGCCTTCGTCTCGCCGGCCGA
TCGCATGATCGGCCAGTACCTGATCGATCTCGTCGACGAGGCCGGCTATCTGCCGGTCGATCTCGGCCAGGCCGCCGACC
GTCTCGGCGCCGAGCAGGCCGATGTCGATGCGGTGCTCGGCGTGCTGCAGACCTTCGATCCGCCCGGCATCTGCGCGCGC
AATCTGAGTGAGTGTCTTGCGATCCAGCTGCGCGAGCTCGATCGCTACGATCCGGCGATGCAGGCTCTGATCGAGCACCT
CGACTTGTTGGCGAAGCGCGATTTCGCCGCACTTCGCAGGCTGTGCGGCGTCGACGACGAGGACCTCGTCGACATGATCG
GCGAGATCCGCAGGCTCGATCCGAAGCCCGGCCTCAAATTCGGCACCACGCGCACGCAGACCATGGTGCCCGACGTCTAT
GTGCGTCCGGGCCCGGATGGCGGCTGGCTGGTCGAGCTCAACAGCGACACGCTGCCGCGCGTCCTGGTGAACCAGGTCTA
TTACTCCGAGCTGTCGAAGACGATCCGCAAGGACGGCGACAAATCCTACTTCACCGATTGCCTGCAGAACGCGACGTGGC
TGGTGCGCGCGCTCGACCAGCGCGCGCGCACGATCCTGAAGGTCGCGACCGAGATCGTGCGGCAGCAGGACGGCTTCTTC
ACTCATGGCGTCGCACATCTCCGGCCCTTGAACCTGAAGGCCGTTGCGGATGCGATCCAGATGCACGAATCGACGGTGTC
GCGCGTCACCGCCAACAAATACATGGCGACCAATCGCGGAACCTTCGAGCTGAAATATTTCTTCACCGCTTCGATCGCCT
CGGCCGATGGCGGCGAGGCGCATTCAGCCGAAGCAGTGCGGCATCACATCAAGCAACTGATCGATGCGGAAGATCCCTCC
GCAATTCTCTCCGACGATACCATCGTCGAGAAGCTGCGCGCTGCCGGCATCGACATCGCCCGCCGCACGGTCGCGAAATA
CCGGGAGGCGATGCGGATTCCGTCGTCGGTCCAGCGCCGCCGCGATAAGCAGAGCATGCTGGCCCATGCCCTGTCGTCGC
CTGCCAGCGCCGACCGCACCCGGGATACCGCGTCAGTTTGA

Upstream 100 bases:

>100_bases
AGCGCGCCATACTCCGTTTTTCTTATTCGTCAAGCCGTGTACATCGTCCGCAGATTAGGATAAGCAAGAATTGGACCAGT
AAGGGAATGTTCTTGCTCCC

Downstream 100 bases:

>100_bases
CCGCTACGGTCAGTTTGACCGTGCCCCGCGATTGCGCCTTAACCAAATAGGCTTAGTGTCATACTCGGTTCACATGAATC
GAGGCGACACATGACCCTGC

Product: RNA polymerase factor sigma-54

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 546; Mature: 545

Protein sequence:

>546_residues
MALTQRLEFRQSQSLVMTPQLMQAIKLLQLSNLDLSTFVEEELERNPLLERANDGPEAPVAGEQQMAERGEFSEGGEASG
DDFGDGAGSSGGESYESAPEDWMSRDLGSRTEIEQTLDTGLDNVFSEEPAEAAARNAQDAAPTTYTEWGGGASGDEDYNL
EAFVAAETTLSDHLAEQAAVAFVSPADRMIGQYLIDLVDEAGYLPVDLGQAADRLGAEQADVDAVLGVLQTFDPPGICAR
NLSECLAIQLRELDRYDPAMQALIEHLDLLAKRDFAALRRLCGVDDEDLVDMIGEIRRLDPKPGLKFGTTRTQTMVPDVY
VRPGPDGGWLVELNSDTLPRVLVNQVYYSELSKTIRKDGDKSYFTDCLQNATWLVRALDQRARTILKVATEIVRQQDGFF
THGVAHLRPLNLKAVADAIQMHESTVSRVTANKYMATNRGTFELKYFFTASIASADGGEAHSAEAVRHHIKQLIDAEDPS
AILSDDTIVEKLRAAGIDIARRTVAKYREAMRIPSSVQRRRDKQSMLAHALSSPASADRTRDTASV

Sequences:

>Translated_546_residues
MALTQRLEFRQSQSLVMTPQLMQAIKLLQLSNLDLSTFVEEELERNPLLERANDGPEAPVAGEQQMAERGEFSEGGEASG
DDFGDGAGSSGGESYESAPEDWMSRDLGSRTEIEQTLDTGLDNVFSEEPAEAAARNAQDAAPTTYTEWGGGASGDEDYNL
EAFVAAETTLSDHLAEQAAVAFVSPADRMIGQYLIDLVDEAGYLPVDLGQAADRLGAEQADVDAVLGVLQTFDPPGICAR
NLSECLAIQLRELDRYDPAMQALIEHLDLLAKRDFAALRRLCGVDDEDLVDMIGEIRRLDPKPGLKFGTTRTQTMVPDVY
VRPGPDGGWLVELNSDTLPRVLVNQVYYSELSKTIRKDGDKSYFTDCLQNATWLVRALDQRARTILKVATEIVRQQDGFF
THGVAHLRPLNLKAVADAIQMHESTVSRVTANKYMATNRGTFELKYFFTASIASADGGEAHSAEAVRHHIKQLIDAEDPS
AILSDDTIVEKLRAAGIDIARRTVAKYREAMRIPSSVQRRRDKQSMLAHALSSPASADRTRDTASV
>Mature_545_residues
ALTQRLEFRQSQSLVMTPQLMQAIKLLQLSNLDLSTFVEEELERNPLLERANDGPEAPVAGEQQMAERGEFSEGGEASGD
DFGDGAGSSGGESYESAPEDWMSRDLGSRTEIEQTLDTGLDNVFSEEPAEAAARNAQDAAPTTYTEWGGGASGDEDYNLE
AFVAAETTLSDHLAEQAAVAFVSPADRMIGQYLIDLVDEAGYLPVDLGQAADRLGAEQADVDAVLGVLQTFDPPGICARN
LSECLAIQLRELDRYDPAMQALIEHLDLLAKRDFAALRRLCGVDDEDLVDMIGEIRRLDPKPGLKFGTTRTQTMVPDVYV
RPGPDGGWLVELNSDTLPRVLVNQVYYSELSKTIRKDGDKSYFTDCLQNATWLVRALDQRARTILKVATEIVRQQDGFFT
HGVAHLRPLNLKAVADAIQMHESTVSRVTANKYMATNRGTFELKYFFTASIASADGGEAHSAEAVRHHIKQLIDAEDPSA
ILSDDTIVEKLRAAGIDIARRTVAKYREAMRIPSSVQRRRDKQSMLAHALSSPASADRTRDTASV

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of the nitrogen fixation genes [H]

COG id: COG1508

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma54 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-54 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789594, Length=528, Percent_Identity=37.8787878787879, Blast_Score=328, Evalue=5e-91,

Paralogues:

None

Copy number: 70 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000394
- InterPro:   IPR007046
- InterPro:   IPR007634 [H]

Pfam domain/function: PF00309 Sigma54_AID; PF04963 Sigma54_CBD; PF04552 Sigma54_DBD [H]

EC number: NA

Molecular weight: Translated: 59858; Mature: 59727

Theoretical pI: Translated: 4.39; Mature: 4.39

Prosite motif: PS00717 SIGMA54_1 ; PS00718 SIGMA54_2 ; PS50044 SIGMA54_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALTQRLEFRQSQSLVMTPQLMQAIKLLQLSNLDLSTFVEEELERNPLLERANDGPEAPV
CCCHHHHHHHHCCCEEECHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHCCCCCCCCCC
AGEQQMAERGEFSEGGEASGDDFGDGAGSSGGESYESAPEDWMSRDLGSRTEIEQTLDTG
CCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHH
LDNVFSEEPAEAAARNAQDAAPTTYTEWGGGASGDEDYNLEAFVAAETTLSDHLAEQAAV
HHHHHCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHH
AFVSPADRMIGQYLIDLVDEAGYLPVDLGQAADRLGAEQADVDAVLGVLQTFDPPGICAR
HHCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHH
NLSECLAIQLRELDRYDPAMQALIEHLDLLAKRDFAALRRLCGVDDEDLVDMIGEIRRLD
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCC
PKPGLKFGTTRTQTMVPDVYVRPGPDGGWLVELNSDTLPRVLVNQVYYSELSKTIRKDGD
CCCCCCCCCCCCCEECCCEEEEECCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCC
KSYFTDCLQNATWLVRALDQRARTILKVATEIVRQQDGFFTHGVAHLRPLNLKAVADAIQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCHHHHCCCCHHHHHHHHH
MHESTVSRVTANKYMATNRGTFELKYFFTASIASADGGEAHSAEAVRHHIKQLIDAEDPS
HHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCC
AILSDDTIVEKLRAAGIDIARRTVAKYREAMRIPSSVQRRRDKQSMLAHALSSPASADRT
HHHCCHHHHHHHHHHCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCC
RDTASV
CCCCCC
>Mature Secondary Structure 
ALTQRLEFRQSQSLVMTPQLMQAIKLLQLSNLDLSTFVEEELERNPLLERANDGPEAPV
CCHHHHHHHHCCCEEECHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHCCCCCCCCCC
AGEQQMAERGEFSEGGEASGDDFGDGAGSSGGESYESAPEDWMSRDLGSRTEIEQTLDTG
CCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHH
LDNVFSEEPAEAAARNAQDAAPTTYTEWGGGASGDEDYNLEAFVAAETTLSDHLAEQAAV
HHHHHCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHH
AFVSPADRMIGQYLIDLVDEAGYLPVDLGQAADRLGAEQADVDAVLGVLQTFDPPGICAR
HHCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHH
NLSECLAIQLRELDRYDPAMQALIEHLDLLAKRDFAALRRLCGVDDEDLVDMIGEIRRLD
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCC
PKPGLKFGTTRTQTMVPDVYVRPGPDGGWLVELNSDTLPRVLVNQVYYSELSKTIRKDGD
CCCCCCCCCCCCCEECCCEEEEECCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCC
KSYFTDCLQNATWLVRALDQRARTILKVATEIVRQQDGFFTHGVAHLRPLNLKAVADAIQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCHHHHCCCCHHHHHHHHH
MHESTVSRVTANKYMATNRGTFELKYFFTASIASADGGEAHSAEAVRHHIKQLIDAEDPS
HHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCC
AILSDDTIVEKLRAAGIDIARRTVAKYREAMRIPSSVQRRRDKQSMLAHALSSPASADRT
HHHCCHHHHHHHHHHCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCC
RDTASV
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1991712; 12597275 [H]