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The map label for this gene is hupB [H]

Identifier: 146301646

GI number: 146301646

Start: 4653025

End: 4654761

Strand: Direct

Name: hupB [H]

Synonym: Fjoh_3907

Alternate gene names: 146301646

Gene position: 4653025-4654761 (Clockwise)

Preceding gene: 146301645

Following gene: 146301647

Centisome position: 76.32

GC content: 39.32

Gene sequence:

>1737_bases
ATGGCAGAGAGAATAGTTGTAGACCCAATTACAAGAATAGAAGGACATTTAAGAGCCGAAGTAGAAATCTCAGACGGCAC
TATAAAAGAAGCCTTCTTATCTTCAACAATGGTAAGAGGTCTGGAGAATATTGTAAAAGACAGAAACCCTAAAGATGTCT
GGGCGTTTGTACAAAGAACTTGTGGTGTTTGTACTTCGACTCATGCTACAGCATCTGTAAGGGCGGTTGAAGATGCACTT
GGAATTGTCGTTCCTCCAAATGCAGAAATAGTCAGAAATATTATGCTGGGTGCTTTGTATTTACACGATCACGTAGTGCA
TTTTTATCATTTGCATGCTTTTGACTGGGTAGATGTTTTGAGCGGTTTAAATGCAGATCCTGTAAAAACTTCACAATTGG
CACAGTCCATTTCAAATTGGCCAAAAAGTTCGCCGGGTTATTTTTCAGATTTACAAAAACGATTAAAAAAGTTTGTTGCC
AGCGGTCAGTTAGGAATTTTTGCCAATGGTTATTGGGGACATCCGCAGATGAAATTACCTCCCGAAGCCAATTTAATGGC
TACAGCCCATTACCTTGAAGCTCTGGAATGGCAAAAGGAAATTGTAAAAGTACATGCCATTTTTGGAGGTAAAAATCCGC
ATCCAAACTTTTTAGTGGGCGGTATGGCGTGTTCTATTAATCTTGATGATGCAAGCGGTTTAAATGCAGAAAGACTTGCT
TTTGTGAGACAGCTGCTGGAAGAAGGAAAACGTTTTGTAGAACAAGTTTACCTTCCGGATGTACTTGCTATTGCTGGTTA
TTATAAAGACTGGGGCGCTATTGGAGGCTTTCATAACTTTATGACTTTTGGCGATTTTCCAACACAGGGTCATAATAATA
CCAACAATGATACTTTTAAATTTCCAGCGGGAGTTATTTTAAACAAAGACTTGACAAAAGTTCATGATTTAGATTTAAGA
GATTTAAAAACGGTAGAAGAATATGTAAACAATTCCTGGTATGATTATGAAGGAGACGGAAATTCAGGAAGACAGCCGTG
GTCTGGAGAAACAAAAATAAATTATACGGGGCCAAAACCGCCTTACACTCATTTAAATGTTGACGAAAAATACAGTTTCA
TTAAAACACCAAGATGGAAAGGCCACGCTATGGAAGTCGGTCCGCTTGCAAGAATGCTGGTTGGTTATGCATCTGGGAGA
GAAGAATTTAAAGATATCGTAGACGGTGCTTTATCAAAACTGCAGATTCCTGCCGCTGCTTTATTCTCGACTTTAGGAAG
AACTGCTGCAAGAGCCTTAGAATCGCAATTAGTAGCCAATTGGAATCTGGAGTTTTTTGATGATTTAATTAAAAACATCA
AAAACGGCGATACTAAAATGGCCAATATGGAAAAATGGGAAACCAATACCTGGCCGAAAGAATCTCAGGGAGTTGGATTG
GTAGAAGCACCGCGAGGCGCTTTGAGCCACTGGATTGTAATAAAAGATGCTAAAGTTGCTAATTATCAGCAGGTCGTGCC
GTCAACGTGGAATGCTTCTCCAAAAGATCCAAAAGGACAGCGATCTCCTTATGAAAGTACTTTATTAAATACGCCAATTG
CAAATCCAGAACTGCCTCTTGAAATTATTAGAACCATACATTCCTTTGATCCCTGCATTGCCTGTGCCGTGCATTTGTAC
GACGAAAATGGCGATATCATTAAAGAAGTAAATGACATAACTATCTGCACCGTTTAA

Upstream 100 bases:

>100_bases
CTTTCGAATGTATCTAAAAGAAAAGAATTAAATCAGAGAATTAGTCAGGGAACTGAAAACGAAAAACACTTAGAGGATTT
ATAACCTATATAGAAATAAA

Downstream 100 bases:

>100_bases
TTTAAACTGAACAGTATGCCAACAAAAACTAACGATTACAAAAGAGCTTATATCTGGCAGTTACCCGTACGAATTTTTCA
TTGGGTAAATGCCTGGGCTA

Product: nickel-dependent hydrogenase, large subunit

Products: NA

Alternate protein names: Hydrogenlyase; Membrane-bound hydrogenase large subunit [H]

Number of amino acids: Translated: 578; Mature: 577

Protein sequence:

>578_residues
MAERIVVDPITRIEGHLRAEVEISDGTIKEAFLSSTMVRGLENIVKDRNPKDVWAFVQRTCGVCTSTHATASVRAVEDAL
GIVVPPNAEIVRNIMLGALYLHDHVVHFYHLHAFDWVDVLSGLNADPVKTSQLAQSISNWPKSSPGYFSDLQKRLKKFVA
SGQLGIFANGYWGHPQMKLPPEANLMATAHYLEALEWQKEIVKVHAIFGGKNPHPNFLVGGMACSINLDDASGLNAERLA
FVRQLLEEGKRFVEQVYLPDVLAIAGYYKDWGAIGGFHNFMTFGDFPTQGHNNTNNDTFKFPAGVILNKDLTKVHDLDLR
DLKTVEEYVNNSWYDYEGDGNSGRQPWSGETKINYTGPKPPYTHLNVDEKYSFIKTPRWKGHAMEVGPLARMLVGYASGR
EEFKDIVDGALSKLQIPAAALFSTLGRTAARALESQLVANWNLEFFDDLIKNIKNGDTKMANMEKWETNTWPKESQGVGL
VEAPRGALSHWIVIKDAKVANYQQVVPSTWNASPKDPKGQRSPYESTLLNTPIANPELPLEIIRTIHSFDPCIACAVHLY
DENGDIIKEVNDITICTV

Sequences:

>Translated_578_residues
MAERIVVDPITRIEGHLRAEVEISDGTIKEAFLSSTMVRGLENIVKDRNPKDVWAFVQRTCGVCTSTHATASVRAVEDAL
GIVVPPNAEIVRNIMLGALYLHDHVVHFYHLHAFDWVDVLSGLNADPVKTSQLAQSISNWPKSSPGYFSDLQKRLKKFVA
SGQLGIFANGYWGHPQMKLPPEANLMATAHYLEALEWQKEIVKVHAIFGGKNPHPNFLVGGMACSINLDDASGLNAERLA
FVRQLLEEGKRFVEQVYLPDVLAIAGYYKDWGAIGGFHNFMTFGDFPTQGHNNTNNDTFKFPAGVILNKDLTKVHDLDLR
DLKTVEEYVNNSWYDYEGDGNSGRQPWSGETKINYTGPKPPYTHLNVDEKYSFIKTPRWKGHAMEVGPLARMLVGYASGR
EEFKDIVDGALSKLQIPAAALFSTLGRTAARALESQLVANWNLEFFDDLIKNIKNGDTKMANMEKWETNTWPKESQGVGL
VEAPRGALSHWIVIKDAKVANYQQVVPSTWNASPKDPKGQRSPYESTLLNTPIANPELPLEIIRTIHSFDPCIACAVHLY
DENGDIIKEVNDITICTV
>Mature_577_residues
AERIVVDPITRIEGHLRAEVEISDGTIKEAFLSSTMVRGLENIVKDRNPKDVWAFVQRTCGVCTSTHATASVRAVEDALG
IVVPPNAEIVRNIMLGALYLHDHVVHFYHLHAFDWVDVLSGLNADPVKTSQLAQSISNWPKSSPGYFSDLQKRLKKFVAS
GQLGIFANGYWGHPQMKLPPEANLMATAHYLEALEWQKEIVKVHAIFGGKNPHPNFLVGGMACSINLDDASGLNAERLAF
VRQLLEEGKRFVEQVYLPDVLAIAGYYKDWGAIGGFHNFMTFGDFPTQGHNNTNNDTFKFPAGVILNKDLTKVHDLDLRD
LKTVEEYVNNSWYDYEGDGNSGRQPWSGETKINYTGPKPPYTHLNVDEKYSFIKTPRWKGHAMEVGPLARMLVGYASGRE
EFKDIVDGALSKLQIPAAALFSTLGRTAARALESQLVANWNLEFFDDLIKNIKNGDTKMANMEKWETNTWPKESQGVGLV
EAPRGALSHWIVIKDAKVANYQQVVPSTWNASPKDPKGQRSPYESTLLNTPIANPELPLEIIRTIHSFDPCIACAVHLYD
ENGDIIKEVNDITICTV

Specific function: This enzyme recycles the H(2) produced by nitrogenase to increase the production of ATP and to protect nitrogenase against inhibition or damage by O(2) under carbon- or phosphate-limited conditions [H]

COG id: COG0374

COG function: function code C; Ni,Fe-hydrogenase I large subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family [H]

Homologues:

Organism=Escherichia coli, GI1787207, Length=575, Percent_Identity=53.9130434782609, Blast_Score=632, Evalue=0.0,
Organism=Escherichia coli, GI1789368, Length=583, Percent_Identity=46.6552315608919, Blast_Score=520, Evalue=1e-148,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001501
- InterPro:   IPR018194 [H]

Pfam domain/function: PF00374 NiFeSe_Hases [H]

EC number: =1.12.99.6 [H]

Molecular weight: Translated: 64457; Mature: 64326

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS00507 NI_HGENASE_L_1 ; PS00508 NI_HGENASE_L_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAERIVVDPITRIEGHLRAEVEISDGTIKEAFLSSTMVRGLENIVKDRNPKDVWAFVQRT
CCCCEEECCHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
CGVCTSTHATASVRAVEDALGIVVPPNAEIVRNIMLGALYLHDHVVHFYHLHAFDWVDVL
HCCCCCCCCHHHHHHHHHHCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGLNADPVKTSQLAQSISNWPKSSPGYFSDLQKRLKKFVASGQLGIFANGYWGHPQMKLP
CCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCC
PEANLMATAHYLEALEWQKEIVKVHAIFGGKNPHPNFLVGGMACSINLDDASGLNAERLA
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECEEEEEECCCCCCCCHHHHH
FVRQLLEEGKRFVEQVYLPDVLAIAGYYKDWGAIGGFHNFMTFGDFPTQGHNNTNNDTFK
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCEEE
FPAGVILNKDLTKVHDLDLRDLKTVEEYVNNSWYDYEGDGNSGRQPWSGETKINYTGPKP
CCCCEEECCCHHHHHCCCHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCEEEECCCCC
PYTHLNVDEKYSFIKTPRWKGHAMEVGPLARMLVGYASGREEFKDIVDGALSKLQIPAAA
CCCCCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHH
LFSTLGRTAARALESQLVANWNLEFFDDLIKNIKNGDTKMANMEKWETNTWPKESQGVGL
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCHHCCCCCCCCCCCCCCE
VEAPRGALSHWIVIKDAKVANYQQVVPSTWNASPKDPKGQRSPYESTLLNTPIANPELPL
EECCCCCHHEEEEEECCCCCCHHHHCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCH
EIIRTIHSFDPCIACAVHLYDENGDIIKEVNDITICTV
HHHHHHHCCCHHHEEEEEEECCCCCHHHHCCCEEEEEC
>Mature Secondary Structure 
AERIVVDPITRIEGHLRAEVEISDGTIKEAFLSSTMVRGLENIVKDRNPKDVWAFVQRT
CCCEEECCHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
CGVCTSTHATASVRAVEDALGIVVPPNAEIVRNIMLGALYLHDHVVHFYHLHAFDWVDVL
HCCCCCCCCHHHHHHHHHHCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGLNADPVKTSQLAQSISNWPKSSPGYFSDLQKRLKKFVASGQLGIFANGYWGHPQMKLP
CCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCC
PEANLMATAHYLEALEWQKEIVKVHAIFGGKNPHPNFLVGGMACSINLDDASGLNAERLA
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECEEEEEECCCCCCCCHHHHH
FVRQLLEEGKRFVEQVYLPDVLAIAGYYKDWGAIGGFHNFMTFGDFPTQGHNNTNNDTFK
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCEEE
FPAGVILNKDLTKVHDLDLRDLKTVEEYVNNSWYDYEGDGNSGRQPWSGETKINYTGPKP
CCCCEEECCCHHHHHCCCHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCEEEECCCCC
PYTHLNVDEKYSFIKTPRWKGHAMEVGPLARMLVGYASGREEFKDIVDGALSKLQIPAAA
CCCCCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHH
LFSTLGRTAARALESQLVANWNLEFFDDLIKNIKNGDTKMANMEKWETNTWPKESQGVGL
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCHHCCCCCCCCCCCCCCE
VEAPRGALSHWIVIKDAKVANYQQVVPSTWNASPKDPKGQRSPYESTLLNTPIANPELPL
EECCCCCHHEEEEEECCCCCCHHHHCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCH
EIIRTIHSFDPCIACAVHLYDENGDIIKEVNDITICTV
HHHHHHHCCCHHHEEEEEEECCCCCHHHHCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3054886; 12597275; 8163174 [H]