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The map label for this gene is hupS [H]

Identifier: 146301645

GI number: 146301645

Start: 4651881

End: 4653008

Strand: Direct

Name: hupS [H]

Synonym: Fjoh_3906

Alternate gene names: 146301645

Gene position: 4651881-4653008 (Clockwise)

Preceding gene: 146301639

Following gene: 146301646

Centisome position: 76.3

GC content: 40.96

Gene sequence:

>1128_bases
ATGAAAGATGAAATAAAAGTAAATGAAACCTATTATAACAGCATTGAAAGACAAGGTTATAGCAGGAGAGATTTTTTAAA
ATTTGTTGCTTACATCGGCGCCTATATGGGCGTGCAGAGTTCTGCAATTGGTCAGATTACCAAAGCGCTCGAAAACACCC
CCCGTCTCCCGGTAATCTGGGAACATTTTCAGGAATGCACCTGCTGCAGTGAGTCTTTCATACGATCAGACCATCCTATT
GTTGCCGATATTATTTTAGACAAAATCTCTTTAGATTACACTTTAACACTTATGGCCGCTTCTGGTCATCAGGCCGAAGC
TGCGAAAAAAGCAACTATGGACAAATACAAAGGAGAATACATTCTTTGTGTTGAAGGTTCTGTACCTATGGGAGCTGACG
GAAATTACTGCTGTATAGGCGGAAGAAGTGCTATTGATATTCTAAAAGAATCTGCTGCAGGAGCAAAAGCTATTATTGCC
TGGGGAAGCTGTGCAACAACAGGCTGTGTGCAGGCTGCAAAACCAAATCCTACAGGAGCTGTTCCTATCAATAAAATTAT
TACCGATAAACCTATTATAAATGTTCCGGGCTGTCCGCCAATTGGTGAAGTTATGGCCGGAATTATTGTACACGTTGTCG
CATTTGGAAAACTGCCTGAATTAGACAGTTCTGGTCGTCCAAAAGCTTTTTATTCTAAAAGAGTTCACGACAGCTGTTAT
CGCAGACCTTATTTTGATGCAGGATTATTTGCTGAAAACTTTGATGACGAAAATGCTAAAAAAGGATACTGCCTATACAA
AGTGGGCTGTAAAGGGCCAAGTACTTACAATGCATGCGGTAATATGAAATGGAACGGCGGTGTGAGTTATCCTATTCAAT
CCGGACATGGATGTATTGGCTGCAGTGCCAAAGATTTTTGGGATGCTGGAAGCTTTTACTCAAGAGATACTTCTATAAAT
GCAGGAAGCATCGAGGCCAACGCCGATTTACTGGGCAAAGTTGCTCTTGGTGGTGTTGCCGCAGGAATTGGAGCTCACGC
GATTCTTTCGAATGTATCTAAAAGAAAAGAATTAAATCAGAGAATTAGTCAGGGAACTGAAAACGAAAAACACTTAGAGG
ATTTATAA

Upstream 100 bases:

>100_bases
AAATGTTTAAATAGTTTTGAAAATAGATTCAGTAAAAAGTTCCTCATGCTTTTAGGAACTTTTTTAAATAAACTAAGGCT
TTCTTTAAACCTATTTTAGC

Downstream 100 bases:

>100_bases
CCTATATAGAAATAAAATGGCAGAGAGAATAGTTGTAGACCCAATTACAAGAATAGAAGGACATTTAAGAGCCGAAGTAG
AAATCTCAGACGGCACTATA

Product: hydrogenase (NiFe) small subunit HydA

Products: NA

Alternate protein names: Hydrogenlyase; Membrane-bound hydrogenase small subunit [H]

Number of amino acids: Translated: 375; Mature: 375

Protein sequence:

>375_residues
MKDEIKVNETYYNSIERQGYSRRDFLKFVAYIGAYMGVQSSAIGQITKALENTPRLPVIWEHFQECTCCSESFIRSDHPI
VADIILDKISLDYTLTLMAASGHQAEAAKKATMDKYKGEYILCVEGSVPMGADGNYCCIGGRSAIDILKESAAGAKAIIA
WGSCATTGCVQAAKPNPTGAVPINKIITDKPIINVPGCPPIGEVMAGIIVHVVAFGKLPELDSSGRPKAFYSKRVHDSCY
RRPYFDAGLFAENFDDENAKKGYCLYKVGCKGPSTYNACGNMKWNGGVSYPIQSGHGCIGCSAKDFWDAGSFYSRDTSIN
AGSIEANADLLGKVALGGVAAGIGAHAILSNVSKRKELNQRISQGTENEKHLEDL

Sequences:

>Translated_375_residues
MKDEIKVNETYYNSIERQGYSRRDFLKFVAYIGAYMGVQSSAIGQITKALENTPRLPVIWEHFQECTCCSESFIRSDHPI
VADIILDKISLDYTLTLMAASGHQAEAAKKATMDKYKGEYILCVEGSVPMGADGNYCCIGGRSAIDILKESAAGAKAIIA
WGSCATTGCVQAAKPNPTGAVPINKIITDKPIINVPGCPPIGEVMAGIIVHVVAFGKLPELDSSGRPKAFYSKRVHDSCY
RRPYFDAGLFAENFDDENAKKGYCLYKVGCKGPSTYNACGNMKWNGGVSYPIQSGHGCIGCSAKDFWDAGSFYSRDTSIN
AGSIEANADLLGKVALGGVAAGIGAHAILSNVSKRKELNQRISQGTENEKHLEDL
>Mature_375_residues
MKDEIKVNETYYNSIERQGYSRRDFLKFVAYIGAYMGVQSSAIGQITKALENTPRLPVIWEHFQECTCCSESFIRSDHPI
VADIILDKISLDYTLTLMAASGHQAEAAKKATMDKYKGEYILCVEGSVPMGADGNYCCIGGRSAIDILKESAAGAKAIIA
WGSCATTGCVQAAKPNPTGAVPINKIITDKPIINVPGCPPIGEVMAGIIVHVVAFGKLPELDSSGRPKAFYSKRVHDSCY
RRPYFDAGLFAENFDDENAKKGYCLYKVGCKGPSTYNACGNMKWNGGVSYPIQSGHGCIGCSAKDFWDAGSFYSRDTSIN
AGSIEANADLLGKVALGGVAAGIGAHAILSNVSKRKELNQRISQGTENEKHLEDL

Specific function: This enzyme recycles the H(2) produced by nitrogenase to increase the production of ATP and to protect nitrogenase against inhibition or damage by O(2) under carbon- or phosphate-limited conditions [H]

COG id: COG1740

COG function: function code C; Ni,Fe-hydrogenase I small subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase small subunit family [H]

Homologues:

Organism=Escherichia coli, GI1787206, Length=366, Percent_Identity=55.1912568306011, Blast_Score=404, Evalue=1e-114,
Organism=Escherichia coli, GI1789371, Length=292, Percent_Identity=44.5205479452055, Blast_Score=265, Evalue=4e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR001821
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: =1.12.99.6 [H]

Molecular weight: Translated: 40401; Mature: 40401

Theoretical pI: Translated: 7.33; Mature: 7.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
4.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDEIKVNETYYNSIERQGYSRRDFLKFVAYIGAYMGVQSSAIGQITKALENTPRLPVIW
CCCCEECCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHH
EHFQECTCCSESFIRSDHPIVADIILDKISLDYTLTLMAASGHQAEAAKKATMDKYKGEY
HHHHHHHHHHHHHHCCCCCEEHHHHHHHHCCCEEEEEEEECCCHHHHHHHHHHHHHCCCE
ILCVEGSVPMGADGNYCCIGGRSAIDILKESAAGAKAIIAWGSCATTGCVQAAKPNPTGA
EEEECCCCCCCCCCCEEEECCCHHHHHHHHHCCCCEEEEEECCCCCHHHCCCCCCCCCCC
VPINKIITDKPIINVPGCPPIGEVMAGIIVHVVAFGKLPELDSSGRPKAFYSKRVHDSCY
CCHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHH
RRPYFDAGLFAENFDDENAKKGYCLYKVGCKGPSTYNACGNMKWNGGVSYPIQSGHGCIG
CCCCCCCCCHHCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEE
CSAKDFWDAGSFYSRDTSINAGSIEANADLLGKVALGGVAAGIGAHAILSNVSKRKELNQ
CCCHHHCCCCCCCCCCCCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RISQGTENEKHLEDL
HHHCCCCCCHHHHCC
>Mature Secondary Structure
MKDEIKVNETYYNSIERQGYSRRDFLKFVAYIGAYMGVQSSAIGQITKALENTPRLPVIW
CCCCEECCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHH
EHFQECTCCSESFIRSDHPIVADIILDKISLDYTLTLMAASGHQAEAAKKATMDKYKGEY
HHHHHHHHHHHHHHCCCCCEEHHHHHHHHCCCEEEEEEEECCCHHHHHHHHHHHHHCCCE
ILCVEGSVPMGADGNYCCIGGRSAIDILKESAAGAKAIIAWGSCATTGCVQAAKPNPTGA
EEEECCCCCCCCCCCEEEECCCHHHHHHHHHCCCCEEEEEECCCCCHHHCCCCCCCCCCC
VPINKIITDKPIINVPGCPPIGEVMAGIIVHVVAFGKLPELDSSGRPKAFYSKRVHDSCY
CCHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHH
RRPYFDAGLFAENFDDENAKKGYCLYKVGCKGPSTYNACGNMKWNGGVSYPIQSGHGCIG
CCCCCCCCCHHCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEE
CSAKDFWDAGSFYSRDTSINAGSIEANADLLGKVALGGVAAGIGAHAILSNVSKRKELNQ
CCCHHHCCCCCCCCCCCCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RISQGTENEKHLEDL
HHHCCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1294332 [H]