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The map label for this gene is nepI [H]

Identifier: 146300842

GI number: 146300842

Start: 3665442

End: 3666668

Strand: Direct

Name: nepI [H]

Synonym: Fjoh_3097

Alternate gene names: 146300842

Gene position: 3665442-3666668 (Clockwise)

Preceding gene: 146300840

Following gene: 146300843

Centisome position: 60.12

GC content: 41.56

Gene sequence:

>1227_bases
ATGCATCAAGAAAAAAATATAACCGCAACTGCTGAAAGTAATTATACTGACTCCTATTGGAATGCGGTATTTGCTGTTGC
ACTTGCCGTGGCAGGTTTAATAACATCCGAATTTTTGCCAGTTAGTCTGCTTACGCCTATGGCGGAAGACTTACAGATAA
GCGAAGGAGCGGCAGGTCAGGCAATTTCAATAACGGCAGCTATTGCAATGTTAAGCAGTTTGTTAATCTCATCCATCACC
AAAACTCTTGACAGACGCAGGGTTCTTTTAATTCTCTGCGTGCTGCAGATCACATCAAATCTATTGGTCTTTTGTGCGCC
TAACTTCTCTGTCTTATTGACTGGAAGAGTGCTTTTAGGCATTGGTCTGGGTGGATTTTGGGGAATGCTGGCTGCAACTG
CAATGCGTTTGGTGGCAGAGAAAGAAGTGCAAAAAGCACTTTCTATTATCTACATAGCGGTATCACTGGCTACAGTTATT
GCAGCACCAATGGGCAGTTTTTTAGGAAATTTATTTGGCTGGAGAAATGTTTTTTTAATAGCGGCAGCCTTAGGTTTCAT
TGCCTTTATTTTACAGGCCATCACTTTGCCAAAAATGCCTGTTGACAAGATAACCCCCTTAAAAACGGTAATTAAAGTAC
TGCAGCTCCCTTCTGTTAAATCAGGAATGTTTGCAACACTATTTTCCTTTATGGCTTATGCTGTCCTATTTACTTATCTA
CGACCATTCCTGGAACAAATAACCGGCGTAGGTTCGAATATTTTGTCTCTGATACTGCTAGGTTTTGGTTTGACAAATCT
GATTGGTGCCACCGTTGCCCGCCACCTTCTATTATGGGATTTATACCGTTCATTAGCCTTAATGCCCTTGCTGATGGGAA
TTTCAGCGGTGGGTTTGGTTTTATTTGGAAACATTTCAATTGTAGCCGCACTCCTGATCGGTCTATGGGGAATGGCTTTA
GGTGTTGTGCAGTTAGGCTGGACGGCGTGGCTGACTAAAACCATACCCGACGAAGCAGAAAGCGCCGGAGGAATTCAAAT
AGCAGTAATACAATTGGCTATTATGACAGGTGCAGCAAGCGGCGGTTGGGTATTTGATAATTTAGGAGCAAAAGGTGTAT
TTATCTTCAGCAGTGTTGTTGCCTTTATTGCTGCAAGTGTTGTTTTTCAATCATCATTACGAATCAAAAAATTAAAAAAA
CAAAAAAAGCCTGGCAGTACTTTATAA

Upstream 100 bases:

>100_bases
TTCATGCTAAAATACGAAATATGTGACATTATATCAGTGTTATATGTAACAAATGGAAGAAAATCGCATTATATCTTTGC
ATTATAAAAATTAAGAAAGA

Downstream 100 bases:

>100_bases
AATCTAAATGTCAAAAAGATTTAATACAAACTGCACATTAAACTAATATTAATTCAATCTCAAACATAATGAAAAAAAGA
GCCATCCTAGCTTTATTAGT

Product: major facilitator transporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 408; Mature: 408

Protein sequence:

>408_residues
MHQEKNITATAESNYTDSYWNAVFAVALAVAGLITSEFLPVSLLTPMAEDLQISEGAAGQAISITAAIAMLSSLLISSIT
KTLDRRRVLLILCVLQITSNLLVFCAPNFSVLLTGRVLLGIGLGGFWGMLAATAMRLVAEKEVQKALSIIYIAVSLATVI
AAPMGSFLGNLFGWRNVFLIAAALGFIAFILQAITLPKMPVDKITPLKTVIKVLQLPSVKSGMFATLFSFMAYAVLFTYL
RPFLEQITGVGSNILSLILLGFGLTNLIGATVARHLLLWDLYRSLALMPLLMGISAVGLVLFGNISIVAALLIGLWGMAL
GVVQLGWTAWLTKTIPDEAESAGGIQIAVIQLAIMTGAASGGWVFDNLGAKGVFIFSSVVAFIAASVVFQSSLRIKKLKK
QKKPGSTL

Sequences:

>Translated_408_residues
MHQEKNITATAESNYTDSYWNAVFAVALAVAGLITSEFLPVSLLTPMAEDLQISEGAAGQAISITAAIAMLSSLLISSIT
KTLDRRRVLLILCVLQITSNLLVFCAPNFSVLLTGRVLLGIGLGGFWGMLAATAMRLVAEKEVQKALSIIYIAVSLATVI
AAPMGSFLGNLFGWRNVFLIAAALGFIAFILQAITLPKMPVDKITPLKTVIKVLQLPSVKSGMFATLFSFMAYAVLFTYL
RPFLEQITGVGSNILSLILLGFGLTNLIGATVARHLLLWDLYRSLALMPLLMGISAVGLVLFGNISIVAALLIGLWGMAL
GVVQLGWTAWLTKTIPDEAESAGGIQIAVIQLAIMTGAASGGWVFDNLGAKGVFIFSSVVAFIAASVVFQSSLRIKKLKK
QKKPGSTL
>Mature_408_residues
MHQEKNITATAESNYTDSYWNAVFAVALAVAGLITSEFLPVSLLTPMAEDLQISEGAAGQAISITAAIAMLSSLLISSIT
KTLDRRRVLLILCVLQITSNLLVFCAPNFSVLLTGRVLLGIGLGGFWGMLAATAMRLVAEKEVQKALSIIYIAVSLATVI
AAPMGSFLGNLFGWRNVFLIAAALGFIAFILQAITLPKMPVDKITPLKTVIKVLQLPSVKSGMFATLFSFMAYAVLFTYL
RPFLEQITGVGSNILSLILLGFGLTNLIGATVARHLLLWDLYRSLALMPLLMGISAVGLVLFGNISIVAALLIGLWGMAL
GVVQLGWTAWLTKTIPDEAESAGGIQIAVIQLAIMTGAASGGWVFDNLGAKGVFIFSSVVAFIAASVVFQSSLRIKKLKK
QKKPGSTL

Specific function: Involved in the efflux of purine ribonucleosides, such as guanosine, adenosine and especially inosine. Involved in the resistance to 6-mercaptopurine [H]

COG id: COG2814

COG function: function code G; Arabinose efflux permease

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. DHA1 family. NepI (TC 2.A.1.2.26) subfamily [H]

Homologues:

Organism=Escherichia coli, GI145693198, Length=382, Percent_Identity=45.0261780104712, Blast_Score=308, Evalue=3e-85,
Organism=Escherichia coli, GI1787808, Length=230, Percent_Identity=30.4347826086957, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1787947, Length=255, Percent_Identity=29.8039215686275, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1786595, Length=247, Percent_Identity=23.8866396761134, Blast_Score=75, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 43370; Mature: 43370

Theoretical pI: Translated: 10.23; Mature: 10.23

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHQEKNITATAESNYTDSYWNAVFAVALAVAGLITSEFLPVSLLTPMAEDLQISEGAAGQ
CCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCHHHCCCCCCCCCC
AISITAAIAMLSSLLISSITKTLDRRRVLLILCVLQITSNLLVFCAPNFSVLLTGRVLLG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCEEEHHHHHHHH
IGLGGFWGMLAATAMRLVAEKEVQKALSIIYIAVSLATVIAAPMGSFLGNLFGWRNVFLI
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAALGFIAFILQAITLPKMPVDKITPLKTVIKVLQLPSVKSGMFATLFSFMAYAVLFTYL
HHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
RPFLEQITGVGSNILSLILLGFGLTNLIGATVARHLLLWDLYRSLALMPLLMGISAVGLV
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LFGNISIVAALLIGLWGMALGVVQLGWTAWLTKTIPDEAESAGGIQIAVIQLAIMTGAAS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCEEHHHHHHHHHHCCCC
GGWVFDNLGAKGVFIFSSVVAFIAASVVFQSSLRIKKLKKQKKPGSTL
CCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MHQEKNITATAESNYTDSYWNAVFAVALAVAGLITSEFLPVSLLTPMAEDLQISEGAAGQ
CCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCHHHCCCCCCCCCC
AISITAAIAMLSSLLISSITKTLDRRRVLLILCVLQITSNLLVFCAPNFSVLLTGRVLLG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCEEEHHHHHHHH
IGLGGFWGMLAATAMRLVAEKEVQKALSIIYIAVSLATVIAAPMGSFLGNLFGWRNVFLI
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAALGFIAFILQAITLPKMPVDKITPLKTVIKVLQLPSVKSGMFATLFSFMAYAVLFTYL
HHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
RPFLEQITGVGSNILSLILLGFGLTNLIGATVARHLLLWDLYRSLALMPLLMGISAVGLV
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LFGNISIVAALLIGLWGMALGVVQLGWTAWLTKTIPDEAESAGGIQIAVIQLAIMTGAAS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCEEHHHHHHHHHHCCCC
GGWVFDNLGAKGVFIFSSVVAFIAASVVFQSSLRIKKLKKQKKPGSTL
CCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA