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The map label for this gene is cysL [H]

Identifier: 146300634

GI number: 146300634

Start: 3423992

End: 3424888

Strand: Reverse

Name: cysL [H]

Synonym: Fjoh_2885

Alternate gene names: 146300634

Gene position: 3424888-3423992 (Counterclockwise)

Preceding gene: 146300635

Following gene: 146300633

Centisome position: 56.17

GC content: 31.77

Gene sequence:

>897_bases
ATGGATTTCAGGCTGAAAGTATTTTATACCGTTGCGCTGCGTCTTAATTTCACTAAGGCGGCTACTGAATTGTATATTAC
ACAGCCAGCAGTTTCAAAACATATTCAGGAACTCGAAGAAACCTATAAAACAAAACTTTTTGAACGAAACGGTTCTAAAA
TCGCGTTAACTCCAGCTGGAGAAATTTTGCTAAAGCATACAAAAGAAATTTTCGAAATTTACCGCGAAATAGATTTTGAA
ATGAGTTCCTTTATCAGCGAACGTCAAGGATTACTGAGATTAGGAGCAAGTACAACCATTTCGCAATATATTATTTCTCC
AGTTTTGGCCCGTTTTCATCAAAAGCAGAAAGATATAAAAGTCAATTTGTTAAACGGAAATACAGAACAAATTGAAAATG
CTTTAATTAATAAAGAAATCGAAATTGGAATTGTCGAAGGGCAGTCAAAAAATCAATCTATCAAATACATTCCCTTTTTA
AAAGACGAATTAGTTTTAGTATGCAGTAGTAAAAATTCGTTTGTAAAACAAAATGAAATTTCAGTAAACGATTTAAAATC
AATGAAATTCATTACTCGTGAACGCGGATCTGGAACACTTGAAGTTATAGAATTTGCACTTAAAAATGTCGGTTTAAAAT
TTAGCGACCTGCAGATTGAAATGCAGTTAGGAAGTACAGAAAGTATAAAATCGTATCTTCTCAATTCAGATTGTTTTGCC
TTTATGTCTATTCATGCCGTAAGCAAAGAATTGAAAAACAAAGAATTAATTGTTCTGGATGTTGAGAAATTATCCATAGA
AAGGTACTTTTATATTATCACTTTACTAGGAAAATCTGATCCTTTGTCAGAATTGTTTATTCAAAACATTGCGAATCACT
ATAACTTGAAGTTATAG

Upstream 100 bases:

>100_bases
TTTGAGTATCGACCTGAACTTTTGTTGTAAATTTTGAAATTATTTTATTTTTAAAAGAAATACAGAGAAAAGAAGATTAC
GAATTTAAAAAAATATAATT

Downstream 100 bases:

>100_bases
TCAATTGTATTTTACGATTGGCGATTATCATTTAATCGTTGGAACTTTGCAGGGTAATTATCAATTATCCTATTTTGAAA
ACAAAACAACAATCCGCATC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: CysJI operon transcriptional activator [H]

Number of amino acids: Translated: 298; Mature: 298

Protein sequence:

>298_residues
MDFRLKVFYTVALRLNFTKAATELYITQPAVSKHIQELEETYKTKLFERNGSKIALTPAGEILLKHTKEIFEIYREIDFE
MSSFISERQGLLRLGASTTISQYIISPVLARFHQKQKDIKVNLLNGNTEQIENALINKEIEIGIVEGQSKNQSIKYIPFL
KDELVLVCSSKNSFVKQNEISVNDLKSMKFITRERGSGTLEVIEFALKNVGLKFSDLQIEMQLGSTESIKSYLLNSDCFA
FMSIHAVSKELKNKELIVLDVEKLSIERYFYIITLLGKSDPLSELFIQNIANHYNLKL

Sequences:

>Translated_298_residues
MDFRLKVFYTVALRLNFTKAATELYITQPAVSKHIQELEETYKTKLFERNGSKIALTPAGEILLKHTKEIFEIYREIDFE
MSSFISERQGLLRLGASTTISQYIISPVLARFHQKQKDIKVNLLNGNTEQIENALINKEIEIGIVEGQSKNQSIKYIPFL
KDELVLVCSSKNSFVKQNEISVNDLKSMKFITRERGSGTLEVIEFALKNVGLKFSDLQIEMQLGSTESIKSYLLNSDCFA
FMSIHAVSKELKNKELIVLDVEKLSIERYFYIITLLGKSDPLSELFIQNIANHYNLKL
>Mature_298_residues
MDFRLKVFYTVALRLNFTKAATELYITQPAVSKHIQELEETYKTKLFERNGSKIALTPAGEILLKHTKEIFEIYREIDFE
MSSFISERQGLLRLGASTTISQYIISPVLARFHQKQKDIKVNLLNGNTEQIENALINKEIEIGIVEGQSKNQSIKYIPFL
KDELVLVCSSKNSFVKQNEISVNDLKSMKFITRERGSGTLEVIEFALKNVGLKFSDLQIEMQLGSTESIKSYLLNSDCFA
FMSIHAVSKELKNKELIVLDVEKLSIERYFYIITLLGKSDPLSELFIQNIANHYNLKL

Specific function: Transcriptional activator of the cysJI operon which is involved in sulfur assimilation. Also negatively regulates its own transcription [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788481, Length=270, Percent_Identity=28.1481481481481, Blast_Score=116, Evalue=1e-27,
Organism=Escherichia coli, GI157672245, Length=205, Percent_Identity=27.8048780487805, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI145693105, Length=210, Percent_Identity=28.0952380952381, Blast_Score=95, Evalue=6e-21,
Organism=Escherichia coli, GI87082132, Length=298, Percent_Identity=28.1879194630872, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1788887, Length=206, Percent_Identity=29.126213592233, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI1788748, Length=215, Percent_Identity=23.7209302325581, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1787601, Length=141, Percent_Identity=31.2056737588652, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI87081904, Length=274, Percent_Identity=23.7226277372263, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1788296, Length=220, Percent_Identity=23.6363636363636, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1790399, Length=302, Percent_Identity=20.5298013245033, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1786401, Length=281, Percent_Identity=22.0640569395018, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1787879, Length=197, Percent_Identity=22.8426395939086, Blast_Score=68, Evalue=9e-13,
Organism=Escherichia coli, GI1790262, Length=283, Percent_Identity=22.9681978798587, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1789506, Length=267, Percent_Identity=23.2209737827715, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI2367136, Length=141, Percent_Identity=29.7872340425532, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1786448, Length=167, Percent_Identity=26.9461077844311, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1787806, Length=168, Percent_Identity=24.4047619047619, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1789639, Length=190, Percent_Identity=25.7894736842105, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1789173, Length=165, Percent_Identity=27.8787878787879, Blast_Score=61, Evalue=8e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34164; Mature: 34164

Theoretical pI: Translated: 8.18; Mature: 8.18

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFRLKVFYTVALRLNFTKAATELYITQPAVSKHIQELEETYKTKLFERNGSKIALTPAG
CCEEEEEEEEEEEEEEEHHHHEEEEEECHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHH
EILLKHTKEIFEIYREIDFEMSSFISERQGLLRLGASTTISQYIISPVLARFHQKQKDIK
HHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCEE
VNLLNGNTEQIENALINKEIEIGIVEGQSKNQSIKYIPFLKDELVLVCSSKNSFVKQNEI
EEEECCCHHHHHHHHCCCCEEEEEEECCCCCCCEEEECCCCCCEEEEECCCCCCEECCCC
SVNDLKSMKFITRERGSGTLEVIEFALKNVGLKFSDLQIEMQLGSTESIKSYLLNSDCFA
CHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCEEEEEEEEEECCCHHHHHHHHCCCCHHH
FMSIHAVSKELKNKELIVLDVEKLSIERYFYIITLLGKSDPLSELFIQNIANHYNLKL
HHHHHHHHHHHCCCCEEEEEHHHHHHEEEEEEEEEECCCCCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MDFRLKVFYTVALRLNFTKAATELYITQPAVSKHIQELEETYKTKLFERNGSKIALTPAG
CCEEEEEEEEEEEEEEEHHHHEEEEEECHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHH
EILLKHTKEIFEIYREIDFEMSSFISERQGLLRLGASTTISQYIISPVLARFHQKQKDIK
HHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCEE
VNLLNGNTEQIENALINKEIEIGIVEGQSKNQSIKYIPFLKDELVLVCSSKNSFVKQNEI
EEEECCCHHHHHHHHCCCCEEEEEEECCCCCCCEEEECCCCCCEEEEECCCCCCEECCCC
SVNDLKSMKFITRERGSGTLEVIEFALKNVGLKFSDLQIEMQLGSTESIKSYLLNSDCFA
CHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCEEEEEEEEEECCCHHHHHHHHCCCCHHH
FMSIHAVSKELKNKELIVLDVEKLSIERYFYIITLLGKSDPLSELFIQNIANHYNLKL
HHHHHHHHHHHCCCCEEEEEHHHHHHEEEEEEEEEECCCCCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377 [H]