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The map label for this gene is menD

Identifier: 146300531

GI number: 146300531

Start: 3313652

End: 3315316

Strand: Direct

Name: menD

Synonym: Fjoh_2781

Alternate gene names: 146300531

Gene position: 3313652-3315316 (Clockwise)

Preceding gene: 146300528

Following gene: 146300532

Centisome position: 54.35

GC content: 33.39

Gene sequence:

>1665_bases
ATGATTTACCCCAAAATAGCGCTTGCACAAAGCATTATCGAAATTTGTTCAGCAAAAGGAATTTACAATATAATAATTTC
TCCAGGTTCGCGAAACGCACCTTTGACTATTGGTTTCGCACAAAATCCAAATTTTAAATGTTACAGTATTGCCGATGAAC
GATGCGCTGCATTTTTTGCTTTAGGAATTGCACAGCAAACCCAAAAGCCAGCTGCAGTAGTTTGTACATCCGGATCTGCA
TTGCTAAACTATTATCCGGCTGTGGCCGAAGCTTTTTACAGTCAGATACCTTTAATTGTAATTTCAGCAGACCGCCCGCA
GAGTAAAATTGATATTGGAGACGGACAAACCATTCGTCAGGAAAATGTCTTTTTAAATCATTCTGTTTTTAATGCCAATT
TAACCGAAGATGCTTCTGTAGAAAATGATTTAAAAATTAATAAAGCTATAGAAACCGCTATTCTTCAAAAAGGTCCGGTT
CATATTAATGCACCTTTTGAAGAACCTTTATACCAAACAGTTTCAGAACTTTCGGTTGAGCCTAAAATTACCAATCTGGA
AGAATTCCTCGAAACAAAAATCATCGAAAACGAAGATGAAATTGTTTCAATCTGGAACTCATCCAAAAGAAAATTAATCC
TTATTGGAGGAATAAACGAAGCCAATTCGATCGATGATGCCGTTTTAGAAAACTTTGCCAAAGATCCGTCTATTGTGGTA
CTTACAGAAACAACATCAAATCTGCATCACCCAAGTTTTATTAACAGCATCGACAGCTTAATTACACCTTTTGATGATTC
AGATTTTAAAGAATTGGAGCCGGAAGTCTTAATTACTTTTGGAGGTATGATAGTATCAAAACGAATTAAAGCTTTTCTGC
GAAAATATAAACCTGTACATCACTGGCATATAGATACATTACGTGCTTATGATACATTCAATGCTTTAAGTAAACATTTT
GTAATGGAACCCAATGATTTTTTCAAAAATCTGCTTCCAAAAACAGAATTTGTTACCAGTAAATATTTTTCAAAAATTGA
TAAAATTTACGATTTAAGAAAAATCAAAAAGAAGCAATATTTGTCTAAAATTGGTTTCTCTGATTTTAAAGTATTTGAAA
AAGTAATTGAATCACTTCCAAAAAACAGTCAGCTGCAAATCAGTAACAGTTCAGCCATTCGATATGCACAATTAATTGAA
ATTGATCCTTCAATAGAGGTTTTCTGTAACAGAGGAACAAGCGGTATAGACGGAAGTACTTCGACTGCTGTTGGTGCAGC
TGTTGGAAGTGGTAAACCAAATGTATTTATTACCGGAGATATCAGCTTTTTGTACGACAGCAATGCTTTATGGAACTCTT
ATATACCTAAGAATTTCAAAATTATATTGATTAATAATGGAGGAGGGGGAATCTTTAGAATTTTGCCTGGGCATCAGGAA
AAACCTGTTTTTAATACTTATTTTGAAACTTCGCATCATTTAACAGCAGAGCATTTGGCTAAAATGTACCAATGCAGTTA
TTTTAAAGCAGATGATTTATCTTCTTTAAATGAAAATTTAGAATTACTATACAATTCTAATGAAGCACCCGGAATTTTAG
AAGTCTTTACCCCAACTTTTGAAAATGATGTTATTCTAAAACAATACTTTAAAGAGCTTACATAA

Upstream 100 bases:

>100_bases
TAATATATAAGTTAAATGAGACACGAAGTTTGTAACTTTGTAATTGCAAAAGGAAATTTTTAAGTACTAAATTTTCTCAA
AATATTATACACCATTTAGA

Downstream 100 bases:

>100_bases
TTATTTGTAAGTTAAATATTGTTAAAAATTTGTTTCTGCTTCTAATATTATTTAGATTTGGTTTTATATAACCAATAATT
AAATTTATATATGAGCAAAA

Product: 2-oxoglutarate decarboxylase

Products: NA

Alternate protein names: SEPHCHC synthase; Menaquinone biosynthesis protein menD

Number of amino acids: Translated: 554; Mature: 554

Protein sequence:

>554_residues
MIYPKIALAQSIIEICSAKGIYNIIISPGSRNAPLTIGFAQNPNFKCYSIADERCAAFFALGIAQQTQKPAAVVCTSGSA
LLNYYPAVAEAFYSQIPLIVISADRPQSKIDIGDGQTIRQENVFLNHSVFNANLTEDASVENDLKINKAIETAILQKGPV
HINAPFEEPLYQTVSELSVEPKITNLEEFLETKIIENEDEIVSIWNSSKRKLILIGGINEANSIDDAVLENFAKDPSIVV
LTETTSNLHHPSFINSIDSLITPFDDSDFKELEPEVLITFGGMIVSKRIKAFLRKYKPVHHWHIDTLRAYDTFNALSKHF
VMEPNDFFKNLLPKTEFVTSKYFSKIDKIYDLRKIKKKQYLSKIGFSDFKVFEKVIESLPKNSQLQISNSSAIRYAQLIE
IDPSIEVFCNRGTSGIDGSTSTAVGAAVGSGKPNVFITGDISFLYDSNALWNSYIPKNFKIILINNGGGGIFRILPGHQE
KPVFNTYFETSHHLTAEHLAKMYQCSYFKADDLSSLNENLELLYNSNEAPGILEVFTPTFENDVILKQYFKELT

Sequences:

>Translated_554_residues
MIYPKIALAQSIIEICSAKGIYNIIISPGSRNAPLTIGFAQNPNFKCYSIADERCAAFFALGIAQQTQKPAAVVCTSGSA
LLNYYPAVAEAFYSQIPLIVISADRPQSKIDIGDGQTIRQENVFLNHSVFNANLTEDASVENDLKINKAIETAILQKGPV
HINAPFEEPLYQTVSELSVEPKITNLEEFLETKIIENEDEIVSIWNSSKRKLILIGGINEANSIDDAVLENFAKDPSIVV
LTETTSNLHHPSFINSIDSLITPFDDSDFKELEPEVLITFGGMIVSKRIKAFLRKYKPVHHWHIDTLRAYDTFNALSKHF
VMEPNDFFKNLLPKTEFVTSKYFSKIDKIYDLRKIKKKQYLSKIGFSDFKVFEKVIESLPKNSQLQISNSSAIRYAQLIE
IDPSIEVFCNRGTSGIDGSTSTAVGAAVGSGKPNVFITGDISFLYDSNALWNSYIPKNFKIILINNGGGGIFRILPGHQE
KPVFNTYFETSHHLTAEHLAKMYQCSYFKADDLSSLNENLELLYNSNEAPGILEVFTPTFENDVILKQYFKELT
>Mature_554_residues
MIYPKIALAQSIIEICSAKGIYNIIISPGSRNAPLTIGFAQNPNFKCYSIADERCAAFFALGIAQQTQKPAAVVCTSGSA
LLNYYPAVAEAFYSQIPLIVISADRPQSKIDIGDGQTIRQENVFLNHSVFNANLTEDASVENDLKINKAIETAILQKGPV
HINAPFEEPLYQTVSELSVEPKITNLEEFLETKIIENEDEIVSIWNSSKRKLILIGGINEANSIDDAVLENFAKDPSIVV
LTETTSNLHHPSFINSIDSLITPFDDSDFKELEPEVLITFGGMIVSKRIKAFLRKYKPVHHWHIDTLRAYDTFNALSKHF
VMEPNDFFKNLLPKTEFVTSKYFSKIDKIYDLRKIKKKQYLSKIGFSDFKVFEKVIESLPKNSQLQISNSSAIRYAQLIE
IDPSIEVFCNRGTSGIDGSTSTAVGAAVGSGKPNVFITGDISFLYDSNALWNSYIPKNFKIILINNGGGGIFRILPGHQE
KPVFNTYFETSHHLTAEHLAKMYQCSYFKADDLSSLNENLELLYNSNEAPGILEVFTPTFENDVILKQYFKELT

Specific function: Catalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-ca

COG id: COG1165

COG function: function code H; 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family. MenD subfamily

Homologues:

Organism=Escherichia coli, GI1788599, Length=524, Percent_Identity=28.8167938931298, Blast_Score=200, Evalue=2e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MEND_FLAJ1 (A5FG67)

Other databases:

- EMBL:   CP000685
- RefSeq:   YP_001195122.1
- ProteinModelPortal:   A5FG67
- STRING:   A5FG67
- GeneID:   5093226
- GenomeReviews:   CP000685_GR
- KEGG:   fjo:Fjoh_2781
- eggNOG:   COG1165
- HOGENOM:   HBG443204
- OMA:   FYHDLNG
- ProtClustDB:   CLSK824047
- BioCyc:   FJOH376686:FJOH_2781-MONOMER
- HAMAP:   MF_01659
- InterPro:   IPR004433
- InterPro:   IPR012001
- InterPro:   IPR011766
- TIGRFAMs:   TIGR00173

Pfam domain/function: PF02775 TPP_enzyme_C; PF02776 TPP_enzyme_N

EC number: =2.2.1.9

Molecular weight: Translated: 62026; Mature: 62026

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYPKIALAQSIIEICSAKGIYNIIISPGSRNAPLTIGFAQNPNFKCYSIADERCAAFFA
CCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEEECCCCCEEEEEECHHHHHHHHH
LGIAQQTQKPAAVVCTSGSALLNYYPAVAEAFYSQIPLIVISADRPQSKIDIGDGQTIRQ
HHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCEEECCCCCEEEC
ENVFLNHSVFNANLTEDASVENDLKINKAIETAILQKGPVHINAPFEEPLYQTVSELSVE
CCEEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHCCCC
PKITNLEEFLETKIIENEDEIVSIWNSSKRKLILIGGINEANSIDDAVLENFAKDPSIVV
CCCCCHHHHHHHHHCCCCCCEEEEECCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCEEE
LTETTSNLHHPSFINSIDSLITPFDDSDFKELEPEVLITFGGMIVSKRIKAFLRKYKPVH
EECCCCCCCCCHHHHHHHHHCCCCCCCCHHHCCCCEEEEECHHHHHHHHHHHHHHCCCCE
HWHIDTLRAYDTFNALSKHFVMEPNDFFKNLLPKTEFVTSKYFSKIDKIYDLRKIKKKQY
EEECHHHHHHHHHHHHHHHEECCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LSKIGFSDFKVFEKVIESLPKNSQLQISNSSAIRYAQLIEIDPSIEVFCNRGTSGIDGST
HHHCCCHHHHHHHHHHHHCCCCCEEEECCCCCEEEEEEEEECCCEEEEECCCCCCCCCCC
STAVGAAVGSGKPNVFITGDISFLYDSNALWNSYIPKNFKIILINNGGGGIFRILPGHQE
CHHHHHHCCCCCCCEEEEEEEEEEECCCCCCCCCCCCCEEEEEEECCCCEEEEECCCCCC
KPVFNTYFETSHHLTAEHLAKMYQCSYFKADDLSSLNENLELLYNSNEAPGILEVFTPTF
CCCCHHHHCCCCCCCHHHHHHHHHCCCCCCCCHHHHCCCEEEEECCCCCCCEEEEECCCC
ENDVILKQYFKELT
CCCHHHHHHHHHCC
>Mature Secondary Structure
MIYPKIALAQSIIEICSAKGIYNIIISPGSRNAPLTIGFAQNPNFKCYSIADERCAAFFA
CCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEEECCCCCEEEEEECHHHHHHHHH
LGIAQQTQKPAAVVCTSGSALLNYYPAVAEAFYSQIPLIVISADRPQSKIDIGDGQTIRQ
HHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCEEECCCCCEEEC
ENVFLNHSVFNANLTEDASVENDLKINKAIETAILQKGPVHINAPFEEPLYQTVSELSVE
CCEEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHCCCC
PKITNLEEFLETKIIENEDEIVSIWNSSKRKLILIGGINEANSIDDAVLENFAKDPSIVV
CCCCCHHHHHHHHHCCCCCCEEEEECCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCEEE
LTETTSNLHHPSFINSIDSLITPFDDSDFKELEPEVLITFGGMIVSKRIKAFLRKYKPVH
EECCCCCCCCCHHHHHHHHHCCCCCCCCHHHCCCCEEEEECHHHHHHHHHHHHHHCCCCE
HWHIDTLRAYDTFNALSKHFVMEPNDFFKNLLPKTEFVTSKYFSKIDKIYDLRKIKKKQY
EEECHHHHHHHHHHHHHHHEECCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LSKIGFSDFKVFEKVIESLPKNSQLQISNSSAIRYAQLIEIDPSIEVFCNRGTSGIDGST
HHHCCCHHHHHHHHHHHHCCCCCEEEECCCCCEEEEEEEEECCCEEEEECCCCCCCCCCC
STAVGAAVGSGKPNVFITGDISFLYDSNALWNSYIPKNFKIILINNGGGGIFRILPGHQE
CHHHHHHCCCCCCCEEEEEEEEEEECCCCCCCCCCCCCEEEEEEECCCCEEEEECCCCCC
KPVFNTYFETSHHLTAEHLAKMYQCSYFKADDLSSLNENLELLYNSNEAPGILEVFTPTF
CCCCHHHHCCCCCCCHHHHHHHHHCCCCCCCCHHHHCCCEEEEECCCCCCCEEEEECCCC
ENDVILKQYFKELT
CCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA