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The map label for this gene is czcB [H]

Identifier: 146300482

GI number: 146300482

Start: 3256566

End: 3257651

Strand: Reverse

Name: czcB [H]

Synonym: Fjoh_2732

Alternate gene names: 146300482

Gene position: 3257651-3256566 (Counterclockwise)

Preceding gene: 146300483

Following gene: 146300481

Centisome position: 53.43

GC content: 32.23

Gene sequence:

>1086_bases
ATGAAACATACACTAATTATAGGAATTGCAATAGTAAGTTTATCACTTGCCAGCTGCAAAAAGGAAGTAGAAAATCCTCA
AACCAATACTTCTTTCGCCTTAAGCGATAATATGCTGAAAACTACTACTACGGCAGATGCGAAAACCCAGACAGTGAAAA
ATGAGCTGAGTTTTTATGGAAAAATTACTGCCGATAATAATAAATTAATCGATGTTTATCCGCTTGTGGGCGGAAACGTG
ATTAAAGTAAATGTTGAACTTGGAGATTATGTAAAAAAAGGCCAGGTATTAGCAACCATAAAAAGTACTGATGTCGCTGA
TTTTGAAAAACAATCTATCGATGCAAAAAATGATTTGCTTGTTGCAAAAAACAAACTAAAAGTTGCTCAGGAATTATACG
ACGGAAAATTAAATTCTGAAAGCGATGTTCTTGAAGCAAAATCTGAAGTAAACAAAGCACAGTCACAGCTAAGCAAAATT
CAGGAAACTTATAAAATTTACAATATTAAAGCCGGTTCTATTTACGAAGTAACGGCGCCAATAAGTGGTTTTGTTATTCA
AAAAAGCATTAATCAGGATATGCTTTTACGTTCTGATCGTTCTGAAAATATTTTTGACATTGCAGAAATCAGCGAAGTTT
GGGCAATGGCCAATATTAATGAAATCGACATTAATAAAATAAAACTTGGAATTGATGCCAGCGTAACTACTCTAAGTTAC
CCTGATAAAGTTTTTAAAGGAAAAGTAGATAAAATCTTCAACGTAATTGATCCCGAAACCAAAGCAATGCAGGCGCGTAT
TAAACTTCAAAACCCTGGTTTTTTATTAAAACCGGATATGAATGCTAATATCAAATTATCCTATAATGAGAATGAATCAA
TGATTGCAATTCCAAGCAATGCCATTGTTTTTGATAAAAGTAAAAATTTCGTCATGATCTTTAAAGATCGTCATAACATT
GAAACAAGACAAGTTGAAGTTTACCGAGTTGTAGGTGATACAACTTATATTTCAAGTGGTTTAAAGGAAAATGAAAAAGT
TATTACCAACAATCAATTATTTATTTATCGCGCTTTAAACGATTAA

Upstream 100 bases:

>100_bases
GGCTAATATTCAGGTTAATGAATTGAAGAAAAAATTGGCTTTATCCGGCGAAGAATTAAATACTACTATCAACAAGGATT
TATTTTAAAACTAAATAATA

Downstream 100 bases:

>100_bases
AAGATGAACAAATTTATACGAAATATAATTGCTTTTTCGCTTAAGAATAAAGCATTTACCTTTTTCTGGGTAGGAATATT
GGCTGTTGCAGGATTTATTT

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 361; Mature: 361

Protein sequence:

>361_residues
MKHTLIIGIAIVSLSLASCKKEVENPQTNTSFALSDNMLKTTTTADAKTQTVKNELSFYGKITADNNKLIDVYPLVGGNV
IKVNVELGDYVKKGQVLATIKSTDVADFEKQSIDAKNDLLVAKNKLKVAQELYDGKLNSESDVLEAKSEVNKAQSQLSKI
QETYKIYNIKAGSIYEVTAPISGFVIQKSINQDMLLRSDRSENIFDIAEISEVWAMANINEIDINKIKLGIDASVTTLSY
PDKVFKGKVDKIFNVIDPETKAMQARIKLQNPGFLLKPDMNANIKLSYNENESMIAIPSNAIVFDKSKNFVMIFKDRHNI
ETRQVEVYRVVGDTTYISSGLKENEKVITNNQLFIYRALND

Sequences:

>Translated_361_residues
MKHTLIIGIAIVSLSLASCKKEVENPQTNTSFALSDNMLKTTTTADAKTQTVKNELSFYGKITADNNKLIDVYPLVGGNV
IKVNVELGDYVKKGQVLATIKSTDVADFEKQSIDAKNDLLVAKNKLKVAQELYDGKLNSESDVLEAKSEVNKAQSQLSKI
QETYKIYNIKAGSIYEVTAPISGFVIQKSINQDMLLRSDRSENIFDIAEISEVWAMANINEIDINKIKLGIDASVTTLSY
PDKVFKGKVDKIFNVIDPETKAMQARIKLQNPGFLLKPDMNANIKLSYNENESMIAIPSNAIVFDKSKNFVMIFKDRHNI
ETRQVEVYRVVGDTTYISSGLKENEKVITNNQLFIYRALND
>Mature_361_residues
MKHTLIIGIAIVSLSLASCKKEVENPQTNTSFALSDNMLKTTTTADAKTQTVKNELSFYGKITADNNKLIDVYPLVGGNV
IKVNVELGDYVKKGQVLATIKSTDVADFEKQSIDAKNDLLVAKNKLKVAQELYDGKLNSESDVLEAKSEVNKAQSQLSKI
QETYKIYNIKAGSIYEVTAPISGFVIQKSINQDMLLRSDRSENIFDIAEISEVWAMANINEIDINKIKLGIDASVTTLSY
PDKVFKGKVDKIFNVIDPETKAMQARIKLQNPGFLLKPDMNANIKLSYNENESMIAIPSNAIVFDKSKNFVMIFKDRHNI
ETRQVEVYRVVGDTTYISSGLKENEKVITNNQLFIYRALND

Specific function: CzcA and CzcB together would act in zinc efflux nearly as effectively as the complete czc efflux system (CzcABC). The CzcB protein is thought to funnel zinc cations to the CzcA transport protein [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1786787, Length=298, Percent_Identity=23.8255033557047, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI87081796, Length=288, Percent_Identity=25.3472222222222, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005695
- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 40326; Mature: 40326

Theoretical pI: Translated: 7.49; Mature: 7.49

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHTLIIGIAIVSLSLASCKKEVENPQTNTSFALSDNMLKTTTTADAKTQTVKNELSFYG
CCCEEEEEHHHHHHHHHHHHHHHCCCCCCCEEEEECCEEEEEECCCCHHHHHHHHHEEEE
KITADNNKLIDVYPLVGGNVIKVNVELGDYVKKGQVLATIKSTDVADFEKQSIDAKNDLL
EEEECCCEEEEEEEECCCEEEEEEEEECCHHCCCEEEEEEECCCCCCHHHHCCCCCCCEE
VAKNKLKVAQELYDGKLNSESDVLEAKSEVNKAQSQLSKIQETYKIYNIKAGSIYEVTAP
EEHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCEEEEECC
ISGFVIQKSINQDMLLRSDRSENIFDIAEISEVWAMANINEIDINKIKLGIDASVTTLSY
CCCEEEEECCCCCCEEECCCCCCCEEHHHHHHHHHHCCCCEEECEEEEEECCCEEEEECC
PDKVFKGKVDKIFNVIDPETKAMQARIKLQNPGFLLKPDMNANIKLSYNENESMIAIPSN
CHHHHCCCHHHHHHHCCCCCCEEEEEEEECCCCEEECCCCCCCEEEEECCCCCEEEECCC
AIVFDKSKNFVMIFKDRHNIETRQVEVYRVVGDTTYISSGLKENEKVITNNQLFIYRALN
EEEEECCCCEEEEEECCCCCCEEEEEEEEEECCCHHHHCCCCCCCEEEECCEEEEEEECC
D
C
>Mature Secondary Structure
MKHTLIIGIAIVSLSLASCKKEVENPQTNTSFALSDNMLKTTTTADAKTQTVKNELSFYG
CCCEEEEEHHHHHHHHHHHHHHHCCCCCCCEEEEECCEEEEEECCCCHHHHHHHHHEEEE
KITADNNKLIDVYPLVGGNVIKVNVELGDYVKKGQVLATIKSTDVADFEKQSIDAKNDLL
EEEECCCEEEEEEEECCCEEEEEEEEECCHHCCCEEEEEEECCCCCCHHHHCCCCCCCEE
VAKNKLKVAQELYDGKLNSESDVLEAKSEVNKAQSQLSKIQETYKIYNIKAGSIYEVTAP
EEHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCEEEEECC
ISGFVIQKSINQDMLLRSDRSENIFDIAEISEVWAMANINEIDINKIKLGIDASVTTLSY
CCCEEEEECCCCCCEEECCCCCCCEEHHHHHHHHHHCCCCEEECEEEEEECCCEEEEECC
PDKVFKGKVDKIFNVIDPETKAMQARIKLQNPGFLLKPDMNANIKLSYNENESMIAIPSN
CHHHHCCCHHHHHHHCCCCCCEEEEEEEECCCCEEECCCCCCCEEEEECCCCCEEEECCC
AIVFDKSKNFVMIFKDRHNIETRQVEVYRVVGDTTYISSGLKENEKVITNNQLFIYRALN
EEEEECCCCEEEEEECCCCCCEEEEEEEEEECCCHHHHCCCCCCCEEEECCEEEEEEECC
D
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8829543 [H]