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The map label for this gene is dnaN [H]

Identifier: 146300470

GI number: 146300470

Start: 3242147

End: 3243265

Strand: Reverse

Name: dnaN [H]

Synonym: Fjoh_2720

Alternate gene names: 146300470

Gene position: 3243265-3242147 (Counterclockwise)

Preceding gene: 146300471

Following gene: 146300468

Centisome position: 53.2

GC content: 34.32

Gene sequence:

>1119_bases
ATGAAATTTATAGTATCGAGTTCGTACTTATTAAAACAATTACAAGTATTAGGAAGCGTTATTAACAGTAATAATACACT
TCCTATTTTAGATAACTTTTTATTTGAACTAGACAATAATGAGTTGACAGTTTCGGCTTCAGACCTTGAAACTACAATGT
CTGCTACATTATCAATCGATTCTACAAGTAAAGGAAGCGTTGCTGTACCGGCAAAACTTTTACTTGAAATTTTAAAGACA
TTTCCAGAGCAGCCTTTAACTTTTACAGTTGAAGACAATAATACAGTCGAAATCAGCTCTAATTCAGGAAAATATGCATT
AGCGTATGCTGCCGGAGAAGAATTCCCTAAGTCTGTAAATCTGGAAGATCCGTCTGTAACTTTAGTTCCTGCTGATGTAT
TGGCAACTGCGGTAAGTAAAACCATTTTTGCTGCCGGAAACGATGATTTACGTCCGGTAATGTCTGGAGTTTTCTTCCAG
TTCTCTCCAGAAGGTTTAACTTTTGTTGCTACAGATGCTCATAAATTAGTAAAATATGCACGTGCAGATGTAAAAGCATC
TCAGGTTGCTGATTTTATTATGCCGAAGAAACCTTTAAATATCTTAAAAAGTATTTTAGGAAGTTCTGATGCGGAAGTAA
AAATTGAATACAACGATTCAAATGCGACTTTCTCATTTGACAATTATATCTTAATGTGTCGTTTAATCGACGGAAAATAC
CCTAATTATGAAGCGGTGATTCCTAAAGAAAATCCAAACAAATTATTAATCGATCGTTCTTTATTTTTAAGCTCTGTTCG
TCGTGTTGCGATTTTCTCAAACAAAACTACACACCAAATTCGTTTAAAAATTGCAGGAGCTGAATTAAATGTTTCTGCTG
AAGATATCGATTACTCAAATAAAGCAGAAGAAAGACTAACTTGTGATTATCAAGGTGATGATCTTCAAATTGGTTTTAAC
TCTCGTTTCTTAATCGAAATGCTGACAAACCTTCAATCTGATGTGATTATGTTAGAGATGTCATTACCTAACAGAGCCGG
GATTTTAACTCCTGCTGATGGCTTAGAAGAAGGTGAAACAGTAACAATGTTGGTAATGCCCGTAATGTTAAATAGTTAA

Upstream 100 bases:

>100_bases
TAAAAAAATTGCGAAACTAAGAATGTTTACGATATATTTGTAAAACCTTACTTAGTTACAGAAAAACTGAGAAAAAGAGC
AAAAAATAAAACAAAAGACG

Downstream 100 bases:

>100_bases
CATTAAAGTTTCTTTATGTTACAGGGATTTTTTTTAATTTGAAATTAAAGACATATCATTGTAAAAAAAATATCGCTATT
AACCAACCATTTTTTATACC

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 372; Mature: 372

Protein sequence:

>372_residues
MKFIVSSSYLLKQLQVLGSVINSNNTLPILDNFLFELDNNELTVSASDLETTMSATLSIDSTSKGSVAVPAKLLLEILKT
FPEQPLTFTVEDNNTVEISSNSGKYALAYAAGEEFPKSVNLEDPSVTLVPADVLATAVSKTIFAAGNDDLRPVMSGVFFQ
FSPEGLTFVATDAHKLVKYARADVKASQVADFIMPKKPLNILKSILGSSDAEVKIEYNDSNATFSFDNYILMCRLIDGKY
PNYEAVIPKENPNKLLIDRSLFLSSVRRVAIFSNKTTHQIRLKIAGAELNVSAEDIDYSNKAEERLTCDYQGDDLQIGFN
SRFLIEMLTNLQSDVIMLEMSLPNRAGILTPADGLEEGETVTMLVMPVMLNS

Sequences:

>Translated_372_residues
MKFIVSSSYLLKQLQVLGSVINSNNTLPILDNFLFELDNNELTVSASDLETTMSATLSIDSTSKGSVAVPAKLLLEILKT
FPEQPLTFTVEDNNTVEISSNSGKYALAYAAGEEFPKSVNLEDPSVTLVPADVLATAVSKTIFAAGNDDLRPVMSGVFFQ
FSPEGLTFVATDAHKLVKYARADVKASQVADFIMPKKPLNILKSILGSSDAEVKIEYNDSNATFSFDNYILMCRLIDGKY
PNYEAVIPKENPNKLLIDRSLFLSSVRRVAIFSNKTTHQIRLKIAGAELNVSAEDIDYSNKAEERLTCDYQGDDLQIGFN
SRFLIEMLTNLQSDVIMLEMSLPNRAGILTPADGLEEGETVTMLVMPVMLNS
>Mature_372_residues
MKFIVSSSYLLKQLQVLGSVINSNNTLPILDNFLFELDNNELTVSASDLETTMSATLSIDSTSKGSVAVPAKLLLEILKT
FPEQPLTFTVEDNNTVEISSNSGKYALAYAAGEEFPKSVNLEDPSVTLVPADVLATAVSKTIFAAGNDDLRPVMSGVFFQ
FSPEGLTFVATDAHKLVKYARADVKASQVADFIMPKKPLNILKSILGSSDAEVKIEYNDSNATFSFDNYILMCRLIDGKY
PNYEAVIPKENPNKLLIDRSLFLSSVRRVAIFSNKTTHQIRLKIAGAELNVSAEDIDYSNKAEERLTCDYQGDDLQIGFN
SRFLIEMLTNLQSDVIMLEMSLPNRAGILTPADGLEEGETVTMLVMPVMLNS

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=343, Percent_Identity=26.2390670553936, Blast_Score=149, Evalue=3e-37,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 40842; Mature: 40842

Theoretical pI: Translated: 4.35; Mature: 4.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFIVSSSYLLKQLQVLGSVINSNNTLPILDNFLFELDNNELTVSASDLETTMSATLSID
CEEEECHHHHHHHHHHHHHHHCCCCCEEEHEEEEEEECCCEEEEEHHHHHEEEEEEEEEC
STSKGSVAVPAKLLLEILKTFPEQPLTFTVEDNNTVEISSNSGKYALAYAAGEEFPKSVN
CCCCCCEECCHHHHHHHHHHCCCCCCEEEECCCCEEEEECCCCCEEEEEECCCCCCCCCC
LEDPSVTLVPADVLATAVSKTIFAAGNDDLRPVMSGVFFQFSPEGLTFVATDAHKLVKYA
CCCCCEEEEEHHHHHHHHHHHEEECCCCCHHHHHHCEEEEECCCCEEEEECCHHHHHHHH
RADVKASQVADFIMPKKPLNILKSILGSSDAEVKIEYNDSNATFSFDNYILMCRLIDGKY
HHCCCHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEEECCCCCEEEECCEEEEEEEECCCC
PNYEAVIPKENPNKLLIDRSLFLSSVRRVAIFSNKTTHQIRLKIAGAELNVSAEDIDYSN
CCEEEEECCCCCCEEEEEHHHHHHHHHEEEEECCCCCEEEEEEEECCEEECCHHCCCCCC
KAEERLTCDYQGDDLQIGFNSRFLIEMLTNLQSDVIMLEMSLPNRAGILTPADGLEEGET
CCCCCEEEEECCCEEEEECCCHHHHHHHHCCCCCEEEEEECCCCCCCEECCCCCCCCCCE
VTMLVMPVMLNS
EEEEEEEEHHCC
>Mature Secondary Structure
MKFIVSSSYLLKQLQVLGSVINSNNTLPILDNFLFELDNNELTVSASDLETTMSATLSID
CEEEECHHHHHHHHHHHHHHHCCCCCEEEHEEEEEEECCCEEEEEHHHHHEEEEEEEEEC
STSKGSVAVPAKLLLEILKTFPEQPLTFTVEDNNTVEISSNSGKYALAYAAGEEFPKSVN
CCCCCCEECCHHHHHHHHHHCCCCCCEEEECCCCEEEEECCCCCEEEEEECCCCCCCCCC
LEDPSVTLVPADVLATAVSKTIFAAGNDDLRPVMSGVFFQFSPEGLTFVATDAHKLVKYA
CCCCCEEEEEHHHHHHHHHHHEEECCCCCHHHHHHCEEEEECCCCEEEEECCHHHHHHHH
RADVKASQVADFIMPKKPLNILKSILGSSDAEVKIEYNDSNATFSFDNYILMCRLIDGKY
HHCCCHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEEECCCCCEEEECCEEEEEEEECCCC
PNYEAVIPKENPNKLLIDRSLFLSSVRRVAIFSNKTTHQIRLKIAGAELNVSAEDIDYSN
CCEEEEECCCCCCEEEEEHHHHHHHHHEEEEECCCCCEEEEEEEECCEEECCHHCCCCCC
KAEERLTCDYQGDDLQIGFNSRFLIEMLTNLQSDVIMLEMSLPNRAGILTPADGLEEGET
CCCCCEEEEECCCEEEEECCCHHHHHHHHCCCCCEEEEEECCCCCCCEECCCCCCCCCCE
VTMLVMPVMLNS
EEEEEEEEHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]